id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-260177-xu0elmak	Collins, Arlene R.	Monoclonal antibodies to murine hepatitis virus-4 (strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion	1982-06-30		.txt	text/plain	4156	232	46	title: Monoclonal antibodies to murine hepatitis virus-4 (strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion Abstract Hybridoma cell lines producing monoclonal antibodies to the JHM strain of mouse hepatitis virus-4 (MHV-4) were established. A third set of monoclonal antibodies reacted both in the cytoplasm and on the surface of infected cells and precipitated the 25,000d viral glycoprotein (GP-5) and its precursor VP-6 (23,000d). MHV-4 polypeptide specificities of monoclonal antibodies were determined by immune precipitation of [35S]methionine-labeled viral proteins from infected L-241 cells. Only incubation with monoclonal antibodies to GP-1 resulted in inhibition in plaque and syncytium number (Fig. 5) , indicating involvement of that polypeptide in cell to cell spread of infection by fusion. When we examined our collection of monoclonal antibodies for the ability to inhibit spread of infection, only anti-GP-1 was effective, suggesting that the virion peplomer contains the active site for cell-cell co % fusion.	./cache/cord-260177-xu0elmak.txt	./txt/cord-260177-xu0elmak.txt