id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-262574-gu0930s3	Slagle, Betty L.	Identification and characterization of a mouse mammary tumor virus protein uniquely expressed on the surface of BALB/cV mammary tumor cells	1985-05-31		.txt	text/plain	7912	407	53	The BALB/cV subline was used to study the molecular basis of potential virus-host interactions involving cell surface-expressed MMTV proteins. Cell surface iodination identified virus-specific proteins expressed on BALB/cv primary mammary tumor cells grown in culture. Adsorption experiments have demonstrated that the anti-gp52/gp36 serum reacts specifically with MMTV glycoproteins and envelope-related precursors and does not react with normal cell proteins of BALB/c mammary tissue (Slagle et aQ, 1985) . Primary cultures of a BALB/cV tumor were analyzed for cell surface expression of viral proteins using the same iodination procedure. Primary cultures of BALB/cV tumor cells were starved for 2 hr in methionine-free media and were then metabolically labeled for 1 hr with r5S]Met. Intact cell monolayers were rinsed with cold TBS, placed on ice, and reacted with specific antisera to detect 35S-labeled MMTV proteins expressed on the cell surface. DISCUSSION This report describes a thorough analysis of the expression of MMTV-specific proteins on the surface of BALB/cV mammary tumor cells.	./cache/cord-262574-gu0930s3.txt	./txt/cord-262574-gu0930s3.txt