id author title date pages extension mime words sentences flesch summary cache txt cord-264331-uvi8ucz4 Singh, Shailbala Avian influenza viral nucleocapsid and hemagglutinin proteins induce chicken CD8(+) memory T lymphocytes 2010-04-10 .txt text/plain 5972 295 51 Following ex vivo co-culture with MHC matched B19/B19 APCs infected with H5N9 virus, memory responses were detected in T cell preparations obtained from all chickens receiving plasmids expressing either AIV protein by 3 weeks p.i. Since neither supernatants from the T cells cultured with uninfected APCs nor T cells from PBS inoculated birds cultured with infected MHC matched B19/B19 birds produced IFNγ (data not shown), the memory T lymphocyte activity was considered AIV specific. In order to evaluate the memory T lymphocyte responses of chickens inoculated with infectious AIV, chicks with the B19/B19 haplotype were inoculated with the low pathogenic H5N9/Turkey/Wis/68 strain and blood was collected at 5 weeks p.i. The ex vivo activation of T lymphocytes by AIV infected APCs was determined by the indirect IFNγ assay (Fig. 5) . ./cache/cord-264331-uvi8ucz4.txt ./txt/cord-264331-uvi8ucz4.txt