id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-264359-m9j3pcj1	Vrijsen, R.	Resolution of the major poliovirus capsid polypeptides into doublets	1978-05-15		.txt	text/plain	3862	223	57	The VPl-VP3 group of poliovirion polypeptides can be resolved into multiple components by normal polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulfate (SDS) (Vanden Berghe and Boeye, 1972) ; however, resolution into six components is achieved more easily and reproducibly in the presence of a pH gradient. In summary, fine resolution of the poliovirus capsid polypeptides was achieved by pH gradient electrophoresis and the results were independent of (i) the virus purification or (ii) protein extraction procedures, (iii) the state of maturation (procapsid or mature virion), and (iv) the serotype (type 1 or 2). DISCUSSION The improved resolution of poliovirus capsid polypeptides by pH gradient electrophoresis reported by Vrijsen and Boeye (1978) was confirmed and shown to be independent of the serotype and of the methods used for virus purification and protein extraction.	./cache/cord-264359-m9j3pcj1.txt	./txt/cord-264359-m9j3pcj1.txt