id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-267014-3vi7pgvr	Vennema, H.	Genomic organization and expression of the 3′ end of the canine and feline enteric coronaviruses	1992-11-30		.txt	text/plain	3543	210	59	Abstract The genomic organization at the 3′ end of canine coronavirus (CCV) and feline enteric coronavirus (FECV) was determined by sequence analysis and compared to that of feline infectious peritonitis virus (FIPV) and transmissible gastroenteritis virus (TGEV) of swine. Amplification of cDNA was performed by the polymerase chain reaction (PCR) as described (Kawasaki and Wang, 1989) , after the addition of synthetic oligonucleotide 178, 5'-GATGACACACAGGlTGAG-3', which is identical to the carboxyl-terminus of the nucleocapsid (N) protein gene of FIPV (nucleotides 1945-l 962; Vennema et a/., 1991) . The nucleotide sequences flanking the ORFs 6a and 6b of FIPV and CCV and ORF 7 of TGEV were aligned to design primers for cDNA synthesis and polymerase chain reaction (PCR) amplification. The recombinant expression products were compared to the proteins produced in CCV-, FECV-, and FIPV-infected cells, which were analyzed similarly (Fig. 6) .	./cache/cord-267014-3vi7pgvr.txt	./txt/cord-267014-3vi7pgvr.txt