id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-268416-8hw80qx8	Grunewald, Matthew E.	The coronavirus nucleocapsid protein is ADP-ribosylated	2018-04-01		.txt	text/plain	4795	263	50	While screening for ADP-ribosylated proteins during coronavirus (CoV) infection, we detected a ~55 kDa ADP-ribosylated protein in mouse hepatitis virus (MHV)-infected cells and in virions, which we identified as the viral nucleocapsid (N) protein. To identify potential targets of the CoV macrodomain, we analyzed infected cells for changes in ADP-ribosylation patterns utilizing antibodies specific for ADPr. We focused on cells infected with a murine CoV, mouse hepatitis virus (MHV). If the macrodomain was indeed removing the ADPr from the N protein, we would expect to see increased N protein ADP-ribosylation in cells infected with mutant virus compared to wild-type infected cells. To determine if N protein expressed in the absence of CoV infection could be ADP-ribosylated in cell culture, we transduced Vero cells with VEEV replicon particles (VRPs) encoding the MERS-CoV N protein or control GFP at different MOIs .	./cache/cord-268416-8hw80qx8.txt	./txt/cord-268416-8hw80qx8.txt