id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-273246-4s54jrww	Niu, Shengniao	An infectious RNA with a hepta-adenosine stretch responsible for programmed −1 ribosomal frameshift derived from a full-length cDNA clone of Hibiscus latent Singapore virus	2014-01-20		.txt	text/plain	4213	213	61	title: An infectious RNA with a hepta-adenosine stretch responsible for programmed −1 ribosomal frameshift derived from a full-length cDNA clone of Hibiscus latent Singapore virus In HLSV, a À 1 PRF product, 108 kDa protein, was still detected using transcripts derived from BspHI digested pHLSV-7A, in which the far downstream HLSV sequence was cut off (Fig. 2C , fourth lane from the right). We report here the construction of HLSV biologically active full-length cDNA clones and the identification of a heptaadenosine stretch in HLSV genome as a slippery sequence responsible for À 1 PRF, independent of its downstream pseudoknot structure and the far downstream RNA sequence in vitro. Since monotonous runs of nucleotides can also cause À 2 or þ 1 PRF (Brierley et al., 1992) , we noticed that in the in vitro translation products using HLSV-8A transcripts, a 78 kDa protein was detected (Fig. 2C , first lane from the right).	./cache/cord-273246-4s54jrww.txt	./txt/cord-273246-4s54jrww.txt