id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-279924-09uwhxs9	Plaisted, Warren C.	T cell mediated suppression of neurotropic coronavirus replication in neural precursor cells	2014-01-01		.txt	text/plain	5580	280	48	In this study, we demonstrate that cultured murine NPCs are infected by the neurotropic JHM strain of mouse hepatitis virus (JHMV), which induces acute encephalomyelitis and chronic demyelination when injected intracranially into immunocompetent mice. We co-cultured virus-specific CTLs at diminishing effectorto-target (E:T) ratios with NPCs pulsed with the immunodominant CD8 peptide specific for JHMV spike (S) glycoprotein spanning amino acids 510-518 (S510-518), and treated with IFN-γ to induce MHC class I expression. To confirm the role of IFN-γ as the major cytokine contributing to suppression of JHMV replication in infected NPC cultures, monoclonal antibody blockade against the IFN-γ receptor was performed on NPCs before and during treatment with virusspecific CD4þ T cell enriched media. Impaired expression of MHC class II following IFN-γ-treatment of infected NPCs may be a mechanism employed to subvert detection by infiltrating virus-specific CD4þ T cells.	./cache/cord-279924-09uwhxs9.txt	./txt/cord-279924-09uwhxs9.txt