id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-281237-asnpuami	Garten, Wolfgang	Inhibition of proteolytic activation of influenza virus hemagglutinin by specific peptidyl chloroalkyl ketones	1989-09-30		.txt	text/plain	4043	208	47	The effect of peptidyl chloroalkyl ketones on the activation of the fowl plague virus hemagglutinin by the proteases specific for paired basic residues has been investigated. When virions containing uncleaved hemagglutinin were incubated with lysates of uninfected cells, cleavage was completely inhibited by peptidyl chloroalkyl ketones containing paired basic residues at a concentration of 1 mM. When dibasic peptidyl chloroalkyl ketones were added to infected cell cultures, cleavage of hemagglutinin and multiple cycles of virus replication were inhibited at 10 mM. the hemagglutinins of the pathogenic avian influenza viruses have a cleavage site consisting of several basic amino acids which can be cleaved by endoproteases present in many cells (for review see Klenk and Rott, 1988) . Table 2 shows an experiment in which a cell lysate has been first incubated with AKR-CMK as an inhibitor and subsequently with chromogenic substrates containing also two basic residues at the cleavage site.	./cache/cord-281237-asnpuami.txt	./txt/cord-281237-asnpuami.txt