id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-281820-oltqsd6n	Watanabe, Rie	Heparan sulfate is a binding molecule but not a receptor for CEACAM1-independent infection of murine coronavirus	2007-09-15		.txt	text/plain	3700	183	58	A highly neurovirulent mouse hepatitis virus (MHV) JHMV strain (wt) with receptor (MHVR)-independent infection activity and its low-virulent mutant srr7 without such activity were found to attach to MHVR-negative, non-permissive BHK cells. To identify the molecule that interacts with JHMV, we focused on heparan sulfate (HS) since it works as a receptor of a mutant MHV-rec1 that infects in an MHVR-independent fashion. To evaluate the infectivity of the attached virus, 50 nM of soMHVR was added to the culture of BHK cells inoculated with wt JHMV and srr7 and those cells were further incubated for 14 h at 37°C. Binding of JHMV to HS on the cell surface HS is the major glycosaminoglycan (GAG) found on most cells and was recently reported as an entry receptor for MHV-BHK, a strain that has an extended host range and infects MHVR-negative cells (de Haan et al., 2005) .	./cache/cord-281820-oltqsd6n.txt	./txt/cord-281820-oltqsd6n.txt