id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-284581-fl2nt4ak	Kleine-Weber, Hannah	Spike proteins of novel MERS-coronavirus isolates from North- and West-African dromedary camels mediate robust viral entry into human target cells	2019-07-19		.txt	text/plain	3195	190	58	title: Spike proteins of novel MERS-coronavirus isolates from Northand West-African dromedary camels mediate robust viral entry into human target cells A recent study showed that MERS-CoV found in North/West(Morocco) and West-African (Burkina Faso and Nigeria) dromedary camels are genetically distinct from Arabian viruses and have reduced replicative capacity in human cells, potentially due to amino acid changes in one or more viral proteins. Here, we show that the spike (S) proteins of the prototypic Arabian MERS-CoV strain, human betacoronavirus 2c EMC/2012, and the above stated African MERS-CoV variants do not appreciably differ in expression, DPP4 binding and ability to drive entry into target cells. We employed a previously described vesicular stomatitis virus (VSV)-based pseudotyping system to study MERS-S-driven host cell entry (Kleine-Weber et al., 2018 known to adequately model key aspects of the coronavirus entry process. Host cell entry driven by the S proteins of North/West-and West-African MERS-CoV isolates from dromedary camels is robust.	./cache/cord-284581-fl2nt4ak.txt	./txt/cord-284581-fl2nt4ak.txt