id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-284707-72vx11aq	Leibowitz, Julian L.	Synthesis of virus-specific RNA in permeabilized murine coronavirus-infected cells	1988-09-30		.txt	text/plain	5498	316	53	For mouse hepatitis virus (MHV), one of the most extensively studied coronaviruses, there are seven species of MHV-specific mRNA present in infected cells, the largest of which is indistinguishable from virion RNA (Leibowitz et a/., 1981; Lai et a/., 1981; Spaan et a/., 1981) . In this paper we report the characteristics of a permeabilized cell system and demonstrate that it incorporates ribonucle-otide triphosphates into RNA molecules which appear identical to the virus-specific mRNAs synthesized in MHV-infected cells. Protease inhibitors and RNase inhibitors have both been reported to increase the RNA-dependent RNA polymerase activity present in extracts of West Nile virus-infected cells (Grun and Brinton, 1986) . The kinetics of the development of the MHV-specific RNA polymerase activity over the course of infection was determined in permeabilized cells. In this work we report the development and characterization of a permeabilized cell system for assaying MHV-specific RNA polymerase activity.	./cache/cord-284707-72vx11aq.txt	./txt/cord-284707-72vx11aq.txt