id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-285869-jwflooop	Clementz, Mark A.	Mutation in murine coronavirus replication protein nsp4 alters assembly of double membrane vesicles	2008-05-01		.txt	text/plain	6966	380	56	Here, we studied the role of a putative replicase anchor, nonstructural protein 4 (nsp4), in the assembly of murine coronavirus DMVs. We used reverse genetics to generate infectious clone viruses (icv) with an alanine substitution at nsp4 glycosylation site N176 or N237, or an asparagine to threonine substitution (nsp4-N258T), which is proposed to confer a temperature sensitive phenotype. Coronaviruses, such as mouse hepatitis virus (MHV) and severe acute respiratory syndrome coronavirus (SARS-CoV) that causes severe respiratory illness in humans (Peiris et al., 2004; Stadler et al., 2003) , generate double membrane vesicles (DMVs), which are the sites of viral RNA synthesis (Baker and Denison, 2008; Goldsmith et al., 2004; Gosert et al., 2002; Snijder et al., 2006) . However, at the non-permissive temperature, DMV assembly and mitochondria morphology are disrupted in Alb ts6 icv-infected cells and viral replicase proteins partially localize with mitochondria.	./cache/cord-285869-jwflooop.txt	./txt/cord-285869-jwflooop.txt