id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-286060-92lazxd7	Stohlman, Stephen A.	Synthesis and subcellular localization of the murine coronavirus nucleocapsid protein	1983-10-30		.txt	text/plain	2012	114	55	Studies on the intracellular synthesis of MHV proteins by pulse-chase experiments have shown that the nucleocapsid protein, pp60, is a primary gene product (3, 12) . In addition, two-dimensional nonequilibrium isoelectric focusing of infected cell lysates indicated that the nucleocapsid protein was composed of multiple heterogeneously charged species which are homogeneous in size (1). In examining the kinetics of the appearance of JHMV proteins in infected DBT cells, we noted that the region of the gel which contains the pp60 protein also contained another protein of slightly lower molecular weight (Fig. 1A) . When infected cells were pulse-labeled with [%]methionine for 2 min and then chased with excess unlabeled methionine (20 m&Q for various lengths of time, only ~57 was detected within the pulse interval (Fig. 1B) .	./cache/cord-286060-92lazxd7.txt	./txt/cord-286060-92lazxd7.txt