id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-287620-vuvgi8xx	Butler, Noah	Murine encephalitis caused by HCoV-OC43, a human coronavirus with broad species specificity, is partly immune-mediated	2006-04-10		.txt	text/plain	6665	344	56	HCoV-OC43, harvested from a patient with an upper respiratory tract infection, was originally isolated after passage in human embryonic tracheal organ cultures; this virus caused neurological disease after only one passage in suckling mice and encephalitis within 2-4 passages (McIntosh et al., 1967) (termed HCoV-OC43 NV ). For example, Talbot and co-workers showed, using the mouse-adapted virus after passage in tissue culture cells (termed HCoV-OC43 QUE herein;) that mice infected intranasally with 10 4 -10 5 TCID 50 developed encephalitis if inoculated 8 days but not 21 days postnatally (Jacomy and Talbot, 2003) . HCoV-OC43 QUE was passaged 5-6 times in tissue culture prior to use in mice (personal communication, Dr. Pierre Talbot, INRS-Institut Armand-Frappier, Laval, Quebec) and consequently may be less virulent than virus isolated directly from infected suckling mouse brains. As shown in Fig. 5 , immunocytochemical staining for viral antigen revealed that the tissue culture-adapted virus infected hamster, pig, human, mouse, monkey and cat cells, but not FRT rat epithelium cells.	./cache/cord-287620-vuvgi8xx.txt	./txt/cord-287620-vuvgi8xx.txt