id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-289152-w5ynbewh	Lee, Sang-Myeong	Porcine arterivirus activates the NF-κB pathway through IκB degradation	2005-11-10		.txt	text/plain	8015	424	46	In porcine reproductive and respiratory syndrome virus (PRRSV)-infected cells, NF-κB activation was characterized by translocation of NF-κB from the cytoplasm to the nucleus, increased DNA binding activity, and NF-κB-regulated gene expression. Taken together, these results demonstrated that PRRSV induces nuclear translocation of NF-nB followed by increased DNA binding activity of NF-nB both in MARC-145 cells and PAM cultures. To determine if NF-nB activation by PRRSV was dependent on InBa degradation in MARC-145 cells, the NF-nB pathway was blocked by using an adenovirus vector expressing a dominant negative form of InBa (Ad-InBaDN) which lacks both constitutive (Barroga et al., 1995) and inducible (Brown et al., 1995) phosphorylation sites. This study showed that PRRSV infection resulted in increased nuclear translocation of NF-nB and increased DNA binding activity both in MARC-145 cells and PAM cultures.	./cache/cord-289152-w5ynbewh.txt	./txt/cord-289152-w5ynbewh.txt