id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-289991-wx4rsr4g	Bhowmick, Rahul	Rotavirus infection induces G1 to S phase transition in MA104 cells via Ca(+2)/Calmodulin pathway	2014-03-21		.txt	text/plain	7167	362	52	To assess whether RV modulates expression of CDK inhibitors to regulate cell cycle, whole cell lysates or total RNA of MA104 cells infected with either SA11 (3 moi) or mock infected were subjected to either immunoblotting or real time PCR with p15, p21, p27 specific antibodies or primers, respectively. This results in its inability to bind to and prevent nuclear translocation of E2F ( Fig. 2A) , since E2F in nucleus can activate transcription of several downstream genes like thymidine kinase, thymidine synthase and dihydrofolate reductase (Fig. 2C) , which promote cell cycle progression and DNA replication, nuclear accumulation of E2F by RV during early infection facilitates G 1 /S restriction point modulation in favor of RV replication. CaMKI has been shown to induce G 1 to S phase transition (Skelding et al., 2011) , concurrently activation of CaMKI (phospho CaMKI) was also observed during early RV infection (2-6 hpi) which correlated with increased CaM expression and accumulation of cells in S phase (Fig. 4A) .	./cache/cord-289991-wx4rsr4g.txt	./txt/cord-289991-wx4rsr4g.txt