id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-290282-oxyzndsj	Ortego, Javier	Transmissible gastroenteritis coronavirus gene 7 is not essential but influences in vivo virus replication and virulence	2003-03-30		.txt	text/plain	4287	215	53	Transmissible gastroenteritis coronavirus (TGEV) contains eight overlapping genes that are expressed from a 3′-coterminal nested set of leader-containing mRNAs. To facilitate the genetic manipulation of the viral genome, genes were separated by duplication of transcription regulating sequences (TRSs) and introduction of unique restriction endonuclease sites at the 5′ end of each gene using an infectious cDNA clone. All the rTGEV viruses conserved the modifications engineered in the cDNAs (data not shown), indicating that the ORF separation and the insertion of unique endonuclease restriction sites between genes were stably maintained in the rTGEV genomes. Interestingly, analysis of viral growth in the gut of infected piglets showed a 100-to 5000-fold reduction of recombinant viruses containing one or more restriction sites in relation to the rTGEV-wt virus ( Fig. 5D and E) .	./cache/cord-290282-oxyzndsj.txt	./txt/cord-290282-oxyzndsj.txt