id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-290883-r2744fb3	TORRES, JUAN M.	Induction of Antibodies Protecting against Transmissible Gastroenteritis Coronavirus (TGEV) by Recombinant Adenovirus Expressing TGEV Spike Protein	1995-11-30		.txt	text/plain	7299	390	54	The antisera induced in swine by the Ad5 recombinants expressing the amino-terminal 26% of the spike protein (containing sites C and B) or the full-length spike protein, when mixed with a lethal dose of virus prior to administration to susceptible piglets, delayed or completely prevented the induction of symptoms of disease, respectively. Alternatively, S gene ces are determined rather than titers since in the first fragments were removed from the original plasmid or procedure virus-antibody mixtures are evaluated in the from pSV2X3-TS vectors without SV-40 Pr signal, or withplaque assay without further dilution of the antibody, proout both Pr and polyadenylation sequences, using the viding highly reproducible results and information about restriction endonucleases indicated in Fig. 1 . Infectious Ad-TS recombinants expressing S protein fragments were generated by cotransfecting 293 cells with pFG144K3-TS or pAB14-TS (which carry S gene sequences from TGEV and pFG173 plasmids).	./cache/cord-290883-r2744fb3.txt	./txt/cord-290883-r2744fb3.txt