id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-291192-wm2eyaam	Becares, Martina	Antigenic structures stably expressed by recombinant TGEV-derived vectors	2014-08-09		.txt	text/plain	9535	526	49	Transmissible gastroenteritis virus (TGEV) was engineered to express porcine reproductive and respiratory syndrome virus (PRRSV) small protein domains, as a strategy to improve heterologous gene stability. Recombinant TGEV (rTGEV) vectors have been engineered for dicistronic expression of heterologous genes, such as porcine reproductive and respiratory syndrome virus (PRRSV) GP5 and M proteins (Cruz et al., 2010) , or rotavirus VP2 and VP6, in which formation of rotavirus virus like particles (VLPs) in the cytoplasm of rTGEV infected cells was observed (Enjuanes et al., 2007) . Therefore, heterologous gene size reduction is a promising strategy to achieve stable expression in TGEV-derived vectors and in general in CoVs. This effect could be due to a decrease of the probability of non-homologous recombination in shorter sequences, or to the elimination of protein domains toxically affecting the host cell or rTGEV life cycle.	./cache/cord-291192-wm2eyaam.txt	./txt/cord-291192-wm2eyaam.txt