id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-294056-7e477y1x	La Monica, Nicola	Coronavirus mRNA synthesis: Identification of novel transcription initiation signals which are differentially regulated by different leader sequences	1992-05-31		.txt	text/plain	3341	173	58	Previously, we have identified a transcription initiation site (for mRNA 2-1), which is more efficiently transcribed by viruses containing two copies of UCUAA sequence in the leader RNA than by those with three copies. To understand the mechanism of MHV mRNA transcription, we have attempted to determine whether the differential regulation of transcription initiation by leader RNA containing different UCUAA copy numbers is unique to mRNA 2-l. The analysis of several other recombinant viruses with different genome structures suggested that mRNA 3-l was synthesized only by viruses with a leader RNA containing three copies of UCUAA and gene 3 sequence derived from A59 but not JHM (10, 13). Using various primers representing sequences of different regions of gene 1 of the JHM strain of MHV (2) and a second primer representing the 5'-end of the leader RNA, we detected several PCR products which represented mRNAs initiated from various sites.	./cache/cord-294056-7e477y1x.txt	./txt/cord-294056-7e477y1x.txt