id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-296075-8axbkyyz	Castro, Raymond F.	Differential Antigen Recognition by T Cells from the Spleen and Central Nervous System of Coronavirus-Infected Mice	1996-08-01		.txt	text/plain	1733	88	53	Abstract CD8+cytotoxic T lymphocytes (CTLs) isolated from the central nervous system (CNS) of C57Bl/6 mice acutely infected with mouse hepatitis virus, strain JHM (MHV-JHM), and analyzed in a directex vivocytotoxicity assay recognize two epitopes (H-2Dband H-2Kb-restricted encompassing amino acids 510–518 and 598–605, respectively) within the surface (S) glycoprotein. In this report, the preferential recognition of the H-2Db-restricted epitope is confirmed using splenocytes stimulatedin vitrowith either MHV-JHM-infected MC57 cells or with a cell line expressing the S protein and analyzed in secondary CTL assays. To determine whether these results represent a difference in epitope recognition between the spleen and CNS, secondary CTL assays were performed using spleen cells coated with peptides encompassing the CTL epitopes as stimulators. These assays using peptide-coated splenocytes as stimulators revealed comparable CTL precursor/effector frequencies for the H-2K b -(average 1/1291, range 1/927-1/1542 spleen cells) and H-2D b -restricted (average 1/987, range 1/380-1/1323 spleen cells) epitopes in these mice.	./cache/cord-296075-8axbkyyz.txt	./txt/cord-296075-8axbkyyz.txt