id author title date pages extension mime words sentences flesch summary cache txt cord-296416-q0rsfzgw LAVI, EHUD Syncytia Formation Induced by Coronavirus Infection Is Associated with Fragmentation and Rearrangement of the Golgi Apparatus 1996-07-15 .txt text/plain 4766 232 50 In many 3-5 hr after infection, individual elements of the GA, cells the secretion blocker Brefeldin A (BFA) reversibly which are associated initially with separate microtubuleredistributes membranes and enzymes of the GA back organizing centers in perinuclear areas of fused cells, into the RER, but does not inhibit endocytosis (Doms et congregate in the center of syncytia and form an exal., 1989; Lippincott-Schwartz et al., 1989; Johnston et al., tended network of nondisrupted intact Golgi complexes 1994). In this study, we used organ-Immunohistochemistry elle-specific antibodies, immunohistochemistry, and transmission electron microscopy to examine the fate of Cells, grown on poly-D-lysine-treated coverslips, were the GA during cell fusion and syncytia formation in mouse fixed with 2% paraformaldehyde for 20 min at room tem-L-2 cells infected with MHV-A59 and fusion defective perature, washed three times in PBS, then incubated for mutants. ./cache/cord-296416-q0rsfzgw.txt ./txt/cord-296416-q0rsfzgw.txt