id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-298934-vtrfqozl	Makino, Shinji	Primary structure and translation of a defective interfering rna of murine coronavirus	1988-10-31		.txt	text/plain	5188	298	57	The detection of such RNA intermediates in MHV-infected cells (Baric et a/., 1985 (Baric et a/., , 1987 suggests that coronavirus genomic RNA synthesis involves a discontinuous and nonprocessive mechanism, which may account for the high frequency of recombination via a copy choice mechanism. Since previous oligonucleotide fingerprinting analysis suggested that DlssE RNA contains the leader sequence and the 5' end region of genomic sequence (Makino et al., 1985) cDNA clones were screened by colony hybridization using 5' end-labeled, leader-specific 72-mer, and two cDNA clones F82 and C96, which correspond to the 5' end of genomic RNA of MHV-JHM . Possible secondary structure at the DI RNA rearrangment sites Sequence analysis revealed that DlssE RNA consisted of three noncontiguous regions of MHV-JHM genomic RNA. Furthermore, as previously described for the standard MHV-JHM, the sequence surrounding the junction of leader RNA and the remaining 5'-end genomic sequence also contains a stable secondary structure (Soe eta/., 1987) .	./cache/cord-298934-vtrfqozl.txt	./txt/cord-298934-vtrfqozl.txt