id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-299976-36r794ow	O’Brien, Amornrat	Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines	2018-12-01		.txt	text/plain	6070	306	56	FCoVs are typically grouped into two biotypes (or pathotypes), which have been classified as feline enteric coronavirus (FECV) and feline infectious peritonitis virus (FIPV), based on tissue tropism, disease progression, and genetic markers (reviewed in Kipar and Meli, 2014; Pedersen, 2014 Pedersen, , 2009 , although the range of disease signs and clinical outcomes are likely to extend beyond these two basic definitions. As part of this study, we characterized three feline cell lines-two from the American Type Culture Collection (ATCC) and one from Cornell University-and evaluated the replication kinetics, efficiency of plaque formation, and responsiveness of these cells to interferon (IFN) in order to identify the optimal cell culture conditions for type I FIPV Black. After observing the rapid and uniform development of CPE and release of virus into cell supernatants during infection of AK-D and Fcwf-4 CU cells, we reasoned that these cells would be employable in a standardized plaque assay to consistently determine FIPV Black titer.	./cache/cord-299976-36r794ow.txt	./txt/cord-299976-36r794ow.txt