id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-300470-vgd1ol2z	Conradie, Andelé M.	Establishment of different plasmid only-based reverse genetics systems for the recovery of African horse sickness virus	2016-09-19		.txt	text/plain	7888	366	46	In both of the above reverse genetics systems, recovery of infectious AHSV-4 from plasmid DNA relies on the expression of T7 RNA polymerase within cells transfected with the AHSV-4 cDNA plasmids. The initial AHSV-4 reverse genetics system developed here consists of 10 plasmids, each containing a full-length cDNA copy of single AHSV-4 genome segments flanked by T7 RNA polymerase promoter and HDV ribozyme sequences. However, the same reassortant virus was also recovered successfully by combining the four AHSV-4 dual vectors with two single reverse genetics plasmids, which contained cDNA copies of the S5 and S6 genome segments, respectively. To this end, we have cloned a T7 RNA polymerase expression cassette onto the genetic backbone of the pJAD-S2-S6 dual reverse vector and subsequently demonstrated that AHSV-4 could be recovered in BSR and L929 cells following transfection of the cells with the modified 5-plasmid set.	./cache/cord-300470-vgd1ol2z.txt	./txt/cord-300470-vgd1ol2z.txt