id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-301755-fxfsr9bj	Wang, F.-I.	Sequence analysis of the spike protein gene of murine coronavirus variants: Study of genetic sites affecting neuropathogenicity	1992-02-29		.txt	text/plain	5594	290	54	authors: Wang, F.-I.; Fleming, John O.; Lai, Michael M.C. title: Sequence analysis of the spike protein gene of murine coronavirus variants: Study of genetic sites affecting neuropathogenicity To understand the molecular basis of MHV neuropathogenesis, we studied the spike protein gene sequences of several neutralization-resistant variants of the JHM strain of MHV, which were selected with monoclonal antibodies (MAbs) specific for the S protein. We found that variant 2.2-V-1, which was selected with MAb J.2.2 and primarily caused demyelination, had a single point mutation at nucleotide (NT) 3340, as compared to the parental JHM virus, which predominantly caused encephalitis. The viruses used in this study included several JHM variants which were selected for their resistance to neutralizing MAbs specific for the S protein (Fleming et a/., 1983) .	./cache/cord-301755-fxfsr9bj.txt	./txt/cord-301755-fxfsr9bj.txt