id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-302486-z36hcvrx	Cobo, Fernando	Diagnostic approaches for viruses and prions in stem cell banks	2006-03-30		.txt	text/plain	7276	347	41	Viral and prion contamination of cell cultures and "feeder" cells, which is a common risk in all biotechnological products derived from the cell lines, is the most challenging and potentially serious outcome to address, due to the difficulty involved in virus and prion detection and the potential to cause serious disease in recipients of these cell products. The use of bovine fetal serum in stem cell cultures requires an urgent need for a risk assessment for Transmissible Spongiform Encephalopathies (TSEs) by means of a sensitive and specific test in all products derived from ruminants (U.S. Food and Drugs Administration, 1999; Directive 2004/C 24/ 03). This panel of tests should necessarily include reverse transcriptase detection as a general test for retroviruses, electron microscopy that can detect different kinds of viral particles and characterize many unknown isolates present in cell cultures and molecular techniques like PCR (conventional or real-time) and RT-PCR tests to include all the viruses that we know pose a risk to the product.	./cache/cord-302486-z36hcvrx.txt	./txt/cord-302486-z36hcvrx.txt