id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-302972-imtttzvr	Feldmann, H.	Glycosylation and oligomerization of the spike protein of marburg virus	1991-05-31		.txt	text/plain	1917	116	56	As shown in Fig. 1 B, the electrophoretic mobility of GP was slightly enhanced after incubation with endoglycosidase H (lane 4), and a distinct further increase was obtained by treatment with endoglycosidase F (lane 3) indicating that GP contains AI-glycans of the oligomannosidic, but mainly of the complex type. Glycohydrolase treatments were performed at 37" overnight after denaturing of the proteins by boiling in buffer containing 0.1% SDS, 0.5% octylglucoside, 0.5% P-mercaptoethanol, 50 mM sodium acetate, pH 7.0, and 5 mM EDTA. Growth of virus, labeling, and cross-linking were performed as described in the legend of Fig. 1 and as in A above. To further analyze the composition of the GP complexes, purified [35S]methionine-labeled virus was subjected to solubilization by nonionic detergent and sedimentation on sucrose density gradients, and the polypeptides present in the fractions obtained from the gradients were assayed by polyacrylamide gel electrophoresis under denaturing and reducing conditions. A. Virus non-cross-linked and treated with @-mercaptoethanol (5%, 10 min, 96") prior to gradient centrifugation.	./cache/cord-302972-imtttzvr.txt	./txt/cord-302972-imtttzvr.txt