id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-303238-us3dybue	Kanjanahaluethai, Amornrat	Membrane Topology of Murine Coronavirus Replicase Nonstructural Protein 3	2007-05-01		.txt	text/plain	4789	247	50	The papain-like protease (PLpro) encoded by the coronavirus that causes severe acute respiratory syndrome (SARS-CoV) processes three sites in the replicase polyprotein (Harcourt et al., 2004) , and has recently been shown to have de-ubiquitinating activity (Barretto et al., 2005; Lindner et al., 2005) . To extend these studies of membrane association of coronavirus replicase products, we analyzed the amino acid sequence of MHV-JHM nsp3 (from glycine-833 to glycine-2840) for probability of transmembrane helices using the five different programs designed to search for putative membrane-spanning sequences: Phobius, TMHMM, HMMTOP, SOSUI and TMpred (Fig. 2) . In contrast, when CMMs were added to the mixture, protein products that included all or part of nsp3-TM (PLP2-2485, -2390 and -2258) were detected predominantly in the pelleted fraction, consistent with membrane association (Fig. 3B ).	./cache/cord-303238-us3dybue.txt	./txt/cord-303238-us3dybue.txt