id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-303497-s3zs1oxf	Breuning, Astrid	Characterization of a cold-sensitive (cs) recombinant between two influenza a strains	1983-10-15		.txt	text/plain	3003	166	60	Recombinants between fowl plague virus (FPV) and these strains were obtained by double infection of chick embryo cells either with specific ts mutants of FPV or FPV wild-type and the other prototype strains, and extended plaque purifications as described by Scholtissek et aL (1976) and Rott et aL (1979) . When plaque tests were performed on chick embryo cells at 37 or 40" with recombinants between FPV and other prototype influenza virus strains it was found that the plaque morphology of most of these recombinants was similar to that of FPV. In a single-cycle multiplication experiment (infection at a multiplicity of lo-50 PFU per cell) at 33", the yield of infectious 113/Ho was very low when compared with the multiplication at 37" or with the adapted strain or FPV (Fig. 2) . These results indicate that a step before virus maturation is impeded at 33' in 113/Ho-infected cells, since labeling of viral proteins is already slowed down at this temperature.	./cache/cord-303497-s3zs1oxf.txt	./txt/cord-303497-s3zs1oxf.txt