id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-307396-u6v6bxwj	Liao, Y.	Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein	2006-06-05		.txt	text/plain	6781	326	51	In a recent study, we demonstrated that the SARS-CoV E protein could obviously enhance the membrane permeability of bacterial cells to onitrophenyl-β-D-galactopyranoside and hygromycin B, suggesting that the protein may function as a viroporin (Liao et al., 2004) . Western blotting analysis of cells expressing wild type and most mutant constructs showed specific detection of three species migrating at the range of molecular masses from 14 to 18 kDa under reducing conditions and representing three isoforms of the E protein (Fig. 3a) . The E protein from coronavirus MHV and IBV was previously shown to undergo modification by palmitoylation HeLa cells expressing the Flag-tagged SARS-CoV and IBV E proteins, respectively, were harvested at 12 h posttransfection, broken by 20 stokes with a Dounce cell homogenizer, and fractionated into cytosol (C) and membrane (M) fractions after removal of cell debris and nuclei. Expression of the untagged SARS-CoV E protein in the same cell type also shows very similar Golgi localization patterns as the Flag-tagged protein (Fig. 8a , panels G-I).	./cache/cord-307396-u6v6bxwj.txt	./txt/cord-307396-u6v6bxwj.txt