id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-309919-sm5o0g1c	Eichwald, Catherine	Mammalian orthoreovirus core protein μ2 reorganizes host microtubule-organizing center components.	2020-08-04		.txt	text/plain	1170	81	46	In infected cells, the MRV μ2 protein forms punctae in the enlarged region of the filamentous VFs that are co-localized with γ-tubulin and resistant to nocodazole treatment, and permitted microtubule (MT)-extension, features common to MT-organizing centers (MTOCs). Using a previously established reconstituted VF model, we addressed the functions of MT-components and MTOCs concerning their roles in the formation of filamentous VFs. Indeed, the MTOC markers γ-tubulin and centrin were redistributed within the VF-like structures (VFLS) in a μ2-dependent manner. Here, specific µ2 punctae observed in filamentous VFs are investigated concerning their 89 ability to co-localize with other reovirus proteins and host elements. Our study shows that µ2 90 punctae in VFs co-localize with γ-tubulin, are resistant to nocodazole, and permit MT 91 emergence, common features for MTOCs. Moreover, using the VFLS model, we found that 92 specific µ2/µNS ratios that support filamentous morphology relocalize γ-tubulin and centrin to 93 foci within the VFLS.	./cache/cord-309919-sm5o0g1c.txt	./txt/cord-309919-sm5o0g1c.txt