id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-310218-fky0cm5e	Yoo, Dongwan	The S2 subunit of the spike glycoprotein of bovine coronavirus mediates membrane fusion in insect cells	1991-01-31		.txt	text/plain	2384	124	50	Abstract The hemagglutinin/esterase (HE), spike precursor (S) and the S1 and S2 subunits of the spike precursor protein of bovine coronavirus were expressed in Spodoptera frugiperda (Sf9) cells, and the cell-fusing activity of each recombinant glycoprotein was examined. The hemagglutinin/esterase (HE), spike precursor (S) and the Sl and S2 subunits of the spike precursor protein of bovine coronavirus were expressed in Spodopfera frugperda (Sf9) cells, and the cell-fusing activity of each recombinant glycoprotein was examined. Thus, in order to identify the viral membrane glycoprotein which induces cell fusion by BCV, we expressed the HE, the S, and the Sl and S2 subunits of the S glycoprotein using recombinant baculoviruses, and examined the cell-fusing activity of each recombinant polypeptide. Therefore, it is clear that proteolytic cleavage is required to induce the fusion activity of both the recombinant S polypeptide in insect cells and the authentic S polypeptide produced in BCV-infected cells (14) .	./cache/cord-310218-fky0cm5e.txt	./txt/cord-310218-fky0cm5e.txt