id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-313091-ksrxsdpp	Shirato, Kazuya	Wild-type human coronaviruses prefer cell-surface TMPRSS2 to endosomal cathepsins for cell entry	2017-12-06		.txt	text/plain	4021	217	58	Studies using the ATCC isolate suggest that HCoV-229E enters cells via the late endosome using cathepsin L to cleave S protein, although it can enter cells via the cell surface or early endosome in the presence of transmembrane protease serine 2 (TMPRSS2) or trypsin (Bertram et al., 2013; Kawase et al., 2009) . In the present study, we found that field isolates of HCoV-OC43 and HCoV-HKU1 could be isolated using HBTE-ALI cell culture, and we then used these clinical isolates to assess whether the mode of virus entry found in HCoV-229E was also in play in other HCoVs. For isolation of HCoVs, nasal swabs were collected from outpatients who showed respiratory infection as a cardinal symptom when assessed at a hospital in Tokyo, Japan. To evaluate the entry routes of clinical isolates of HCoVs, viruses were inoculated onto HBTE-ALI in the presence of EST or camostat (10 μM) and the amounts virus that entered were estimated by detecting subgenomic mRNAs using real-time RT-PCR (Fig. 2) .	./cache/cord-313091-ksrxsdpp.txt	./txt/cord-313091-ksrxsdpp.txt