id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-315069-xo4mbxei	Knorr, D. A.	De novo generation of defective interfering RNAs of tomato bushy stunt virus by high multiplicity passage	1991-03-31		.txt	text/plain	5134	250	52	Abstract Defective interfering (DI) RNAs were generated de novo in each of 12 independent isolates of tomato bushy stunt virus (TBSV) upon serial passage at high multiplicities of infection (m.o.i.) in plants, but not in any of 4 additional isolates after 11 serial passages at low m.o.i. The DI RNAs were detected in RNA isolated from virus particles and in 2.3 M LiCl-soluble RNA fractions isolated from inoculated leaves. On each host species, six isolates were passed at high m.o.i. and two control isolates were passed at low m.o.i. In these hosts, DI-free TBSV induces lethal necrosis within 7-l 0 days postinoculation (d.p.i.), except in the presence of DI RNAs which are associated with attenuation and the establishment of persistent infections (Hillman et a/., 1987) . The small RNAs which developed in each of the high m.o.i. isolates hybridized to both 5'-and 3'-proximal TBSV genomic sequences, but generally were larger (-600 bases) than the previously characterized DI 1 RNA species (-400 bases).	./cache/cord-315069-xo4mbxei.txt	./txt/cord-315069-xo4mbxei.txt