id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-315158-f6msh8od	Taguchi, Fumihiro	Comparison of six different murine coronavirus jhm variants by monoclonal antibodies against the e2 glycoprotein	1989-03-31		.txt	text/plain	1620	86	58	Abstract We have examined six different JHMV variants, sp-4 (recloned wt JHMV), cl-2, CNSV, DL, DS, and JHM-X, in terms of the sizes of the mRNA3 and E2 glycoprotein as well as their reactivity to a panel of monoclonal antibodies to the E2 glycoprotein. Recently, we have shown that the highly virulent variant viruses with larger E2 glycoproteins were preferentially isolated from rat brain (19) and cultured astrocytes (20) after infection by wild-type (wt) JHMV which contains a small mRNA3 and E2 glycoprotein. E2 glycoproteins produced by sp-4 and JHM-X, both of which synthesized a small mRNA3, were shown to be approximately 15,000 Da smaller than those produced by the other variant viruses with larger mRNA3.s. No significant differences were observed in the sizes of N proteins produced by the variants. As shown in Fig. 3 , the monoclonal antibodies uniformly had excellent binding to all the viruses tested, with the exception of sp-4 and JHM-X, the two variants with small mRNA3s and E2 proteins.	./cache/cord-315158-f6msh8od.txt	./txt/cord-315158-f6msh8od.txt