id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316134-lkd2mj27	Sungsuwan, Suttipun	Nucleocapsid proteins from other swine enteric coronaviruses differentially modulate PEDV replication	2020-01-15		.txt	text/plain	9236	495	52	Investigation of possible molecular interactions between components of PEDV, PDCoV and TGEV and their influence on replication of each virus would provide a crucial insight into comprehensive understanding of these CoVs. Of all viral proteins, we have chosen to start with the N protein, as it is among the most abundant and ubiquitous structural proteins in infected cells. For viral infection in the transient CoV N expression experiment, VeroE6 cells were transfected with 1 or 2 μg of pCAGGS-PEDV N-Myc, pCAGGS-PDCoV N-HA, pCAGGS-TGEV N-FLAG, or the empty pCAGGS vector and were incubated for 24 h to allow for protein expression. To investigate how this protein-protein interaction might affect PEDV replication, we transiently transfected VeroE6 cells with varying amounts of the pCAGGS plasmid expressing N proteins from either PDCoV or TGEV for 24 h before infection with PEDV-mCherry (MOI = 0.0001) and followed the course of viral replication for each condition.	./cache/cord-316134-lkd2mj27.txt	./txt/cord-316134-lkd2mj27.txt