id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316460-ibprgdh4	Rodríguez-Grille, Javier	Avian reovirus-triggered apoptosis enhances both virus spread and the processing of the viral nonstructural muNS protein	2014-06-17		.txt	text/plain	7493	334	49	To assess whether muNS cleavage is promoted by avian reovirus proteins and/or by changes induced in the host during infection, we compared muNS-to-muNSC conversion in ARV-infected cells with that in transfected cells and in insect cells infected with a muNSexpressing recombinant baculovirus (Fig. 1A ). To verify that this putative caspase recognition sequence is a bona fide cleavage site we first examined whether the electrophoretic mobility of muNS, muNSC and muNSN matched with that of transiently expressed polypeptides comprising muNS residues 1-635, The resulting extracts were immunoprecipitated with polyclonal anti-muNS serum and the imunoprecipitated proteins were resolved on an electrophoresis gel system (8% tricine-SDS-PAGE gel) specifically designed to resolve small peptides (Schägger, 2006) . In the absence of an established reverse genetics system for ARV that would allow us to generate a recombinant virus that expresses an uncleavable muNS protein, like D154A, we examined the effect of Q-VD-OPh on ARV replication in CEF cells, since this compound had been shown to block both apoptosis and muNS processing.	./cache/cord-316460-ibprgdh4.txt	./txt/cord-316460-ibprgdh4.txt