id author title date pages extension mime words sentences flesch summary cache txt cord-319179-gqaxf7mz Denison, M. Identification of putative polymerase gene product in cells infected with murine coronavirus A59 1987-04-30 .txt text/plain 1638 88 59 When infected cells and isolated virions were assayed for this protein by two-dimensional gel electrophoresis, p28 could be detected in infected cells labeled at late times after infection, but not at early times or in purified virions. When infected cells and isolated virions were assayed for this protein by twodimensional gel electrophoresis, p28 could be detected in infected cells labeled at late times after infection, but not at early times or in purified virions. To determine if p28 was a minor protein actually present in virions, infected 17CL-1 cells were labeled with [35S] methionine and virus was purified as described in Fig. 4 . A portion of the labeled virus preparation was analyzed directly by two-dimensional gel electrophoresis whereas a second part was mixed with the [35S] methionine-labeled cell-free products prior to analysis (Fig. 4) . ./cache/cord-319179-gqaxf7mz.txt ./txt/cord-319179-gqaxf7mz.txt