id author title date pages extension mime words sentences flesch summary cache txt cord-321265-il9vbbgk DEN BOON, JOHAN A. Equine Arteritis Virus Subgenomic RNA Transcription: UV Inactivation and Translation Inhibition Studies 1995-11-30 .txt text/plain 3721 222 56 In general, MHV transcription was extremely sensitive to translation inhibition, whereas EAV genomic RNA synthesis became independent ofde novoprotein synthesis late in infection. The replication of SIN involves the synthesis of a EAV-infected BHK-21 cells were UV-irradiated at 6 1 2 hr single 4.1-kb sg RNA (26S) from a well-defined internal promoter on the genome-sized minus-strand template p.i., when RNA synthesis approaches its maximum (van RNA (Ou et al., 1982; Levis et al., 1990) . The UV transcription mapping data showed that the Third, the overall MHV transcription was significantly EAV sg RNAs were not produced by processing of a more dependent on de novo protein synthesis than that genome-length precursor RNA. Protein synthesis was inhibited by the addition of cycloheximide to EAV-infected BHK-21 cells at fully independent transcription system the slopes of the curves in Fig. 2B , which reflect the UV target sizes, should different time points after infection. ./cache/cord-321265-il9vbbgk.txt ./txt/cord-321265-il9vbbgk.txt