id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-322062-nnefbeo6	Tam, Albert W.	Hepatitis E virus (HEV): Molecular cloning and sequencing of the full-length viral genome	1991-11-30		.txt	text/plain	5738	296	49	We now report on the molecular cloning and sequencing of the complete HEV (Burma; B) viral genome together with the deduced amino acid sequences of viral-encoded proteins General perspectives on the genetic organization of the virus, as deduced from sequence and open reading frame analyses, indicate that HEV bears some similarity to the caliciviridae but may represent a new class of nonenveloped RNA virus. Bife was chosen as the RNA source for cDNA synthesis because it contained relatively large numbers of virus particles when HEV cDNA clones were identified from libraries made from randomly primed cyno bile (solid square), or from cyno liver after priming by oligo-dT (solid circle), random sequence hexamers (open circle) and HEV-sequence specific oligonucleotides (open square). The presence of HEV-specific subgenomic RNAs localized to the 3' one-third of the genome suggests that these may be the transcripts from which these 3' end ORFs are expressed and is indicative of a unique expression strategy among nonenveloped positive-sense RNA viruses infecting humans.	./cache/cord-322062-nnefbeo6.txt	./txt/cord-322062-nnefbeo6.txt