id author title date pages extension mime words sentences flesch summary cache txt cord-322084-gkg1059v JEONG, YONG SEOK Coronavirus Transcription Mediated by Sequences Flanking the Transcription Consensus Sequence 1996-03-01 .txt text/plain 6250 302 52 Abstract In our studies of murine coronavirus transcription, we continue to use defective interfering (DI) RNAs of mouse hepatitis virus (MHV) in which we insert a transcription consensus sequence in order to mimic subgenomic RNA synthesis from the nondefective genome. By using PCR-based sequences flanking the same intergenic region between site-directed mutagenesis, a 12-nucleotide-long segenes 6 and 7 do not affect the efficiency of subgenomic quence, TCTAATCTAAAC, was inserted into DI cDNA DI RNA transcription (Makino and Joo, 1993) . For all of the constructs used in Deletion analysis of those MHV DI RNAs that contain this study we sequenced the inserts that were derived the intergenic sequence from gene 6-7 with its naturally from PCR products to confirm the presence of specific occurring flanking sequences showed that reducing the mutations and the absence of extraneous mutations. ./cache/cord-322084-gkg1059v.txt ./txt/cord-322084-gkg1059v.txt