id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-324321-y96x8x3h	Cai, Yingyun	Down-regulation of transcription of the proapoptotic gene BNip3 in cultured astrocytes by murine coronavirus infection	2003-11-10		.txt	text/plain	8454	416	49	Furthermore, treatment of cells with chloroquine, which blocks the acidification of endosomes, significantly inhibited the repression of the BNip3 promoter activity induced by the acidic pH-dependent MHV mutant OBLV60, which enters cells via endocytosis, indicating that the down-regulation of BNip3 expression is mediated by fusion between viral envelope and cell membranes during entry. To establish further the specificity of the down-regulation of BNip3 gene expression, we used vesicular stomatitis virus (VSV) in a parallel experiment, because VSV is also an enveloped RNA virus whose infection has a broad host cell range and is mediated by the interaction between the envelope G protein and cell membranes (Riedel et al., 1984) . By extrapolating the data from the mutant virus, we can envision that fusion between viral envelope and plasma/endosomal membranes during wild-type MHV infection is involved in the down-regulation of BNip3 gene expression.	./cache/cord-324321-y96x8x3h.txt	./txt/cord-324321-y96x8x3h.txt