id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-325179-gsf8ad65	Jarvis, Donald L.	Modification of simian virus 40 large tumor antigen by glycosylation	1985-03-31		.txt	text/plain	7725	450	51	In some experiments, SV40-infected cells were labeled with pS]methionine or 3H-monosaccharides in the presence of excess unlabeled amino acids, excess unlabeled sugars, or TM. Infected cells were starved in glucose-free E-MEM from 20.5 to 21 hr p.i., then labeled for 3 hr using 25 &i/ml [?S]methionine or 100 &i/ml rH]galactose in E-MEM containing different sugar concentrations. A polypeptide of about 88,000 (88K) in apparent MW was detected by immunoprecipitation of pS]methionine-labeled, SV40-infected cell extracts with HAF (Fig. lA, lane 3) . Coomassie blue staining revealed that both large T-ag -and small t-ag were also immunoprecipitated from galactose-labeled infected cell extracts (data not shown). To determine the effect of TM treatment, SV40-infected cells were treated with E-MEM or various concentrations of TM starting at 15 hr pi., then were glucose-starved and labeled with pS]methionine or THlglucosamine from 21 to 24 hr p.i. After extraction, T-ag was immunoprecipitated and analyzed by SDS-PAGE (Fig. 8A) .	./cache/cord-325179-gsf8ad65.txt	./txt/cord-325179-gsf8ad65.txt