id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-325481-uzch2hwd	Simmons, Graham	Different host cell proteases activate the SARS-coronavirus spike-protein for cell-cell and virus-cell fusion	2011-05-01		.txt	text/plain	7139	315	49	For instance, the majority of strains of the murine coronavirus mouse hepatitis virus (MHV) contain Sproteins that are cleaved by furin in infected cells, and these viruses are believed to enter target cells by receptor-dependent, pH-independent fusion with the plasma membrane (de Haan et al., 2004; Nash and Buchmeier, 1997; Qiu et al., 2006) , although some of these findings are controversial (Eifart et al., 2007) . Treatment of cell-bound virus with trypsin was shown to allow infectious SARS-S-driven entry into ammonium chloride-treated cells (Simmons et al., 2005) , indicating that trypsin can functionally replace cathepsin L as a SARS-S-activating protease under these conditions ("trypsin bypass"). The fusion efficiency is then quantified by the addition of virions to leupeptin-treated (to exclude an impact of host cell proteases on SARS-S activation) HeLa cells, which express the EnvA receptor TvA, and which are not susceptible to SARS-S-driven infection (Simmons et al., 2005) .	./cache/cord-325481-uzch2hwd.txt	./txt/cord-325481-uzch2hwd.txt