id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-326688-a1djgqpa	Dubois-Dalcq, Monique E.	Cell tropism and expression of mouse hepatitis viruses (MHV) in mouse spinal cord cultures	1982-06-30		.txt	text/plain	3946	230	54	The replication of three different MHV strains was studied in mouse dissociated spinal cord cultures containing differentiated neurons and nonneuronal cells (NN) (including astrocytes). Cell tropism and maturation of each virus strain was analyzed by immunolabeling methods using antisera to the virion or to purified membrane glycoproteins (E1 and E2) and by electron microscopy (EM). In conclusion, in primary CNS cultures consisting of neurons and NN cells: (1) wt-JHM replicates in both neurons and NN cells but has different effects on these cells; (2) Ts8-JHM exhibits no productive infection of neurons, and in NN cells appears to be defective in assembly and to stimulate membrane synthesis; (3) A59 also shows tropism restricted to NN cells which produce many viruses and display differential distribution of the two virion glycoproteins. DISCUSSION The present study describes how three different MHV strains interact in vitro with cultured neurons and NN cells, including astrocytes, isolated from mouse spinal cords.	./cache/cord-326688-a1djgqpa.txt	./txt/cord-326688-a1djgqpa.txt