id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-329819-dpgexphf	Hu, Weiwei	Epidermal growth factor receptor is a co-factor for transmissible gastroenteritis virus entry	2018-06-04		.txt	text/plain	6765	435	56	IPEC-J2 cells were infected with TGEV (MOI = 2) and cultured for 30 min, then stained for fluorescence microscopy using mouse anti-APN pAb, followed by DyLight 488-conjugated goat anti-mouse IgG and rabbit anti-p-EGFR mAb, followed by DyLight 594-conjugated goat anti-rabbit IgG. Plaque formation in ST cells by the intracellular of infected IPEC-J2 cells showed that APN + EGFR-targeting shRNAs inhibited TGEV entry more significantly (Fig. 3G) . To explore the role of caveolin and clathrin in EGFR internalization early in TGEV infection, we reduced clathrin or caveolin down in normal IPEC-J2 cells through targeting shRNAs, and investigated cell membrane EGFR expression levels during TGEV invasion. We can get to the conclusion that in the early infection stage of TGEV, TGEV particles bound with APN and EGFR, the virus-receptors complex are subsequently internalized by clathrin.	./cache/cord-329819-dpgexphf.txt	./txt/cord-329819-dpgexphf.txt