id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-340983-w219g6qs	Smith, Mary Ellen	Rabies Virus Glycoprotein as a Carrier for Anthrax Protective Antigen	2006-09-01		.txt	text/plain	7400	376	50	Using the rabies virus (RV) envelope protein as a carrier molecule we show here that inactivated RV particles can be utilized to present Bacillus anthracis protective antigen (PA) domain-4 in the viral membrane. Rabies virus is a promising vaccine vector able to induce humoral and cellular immune responses efficiently to foreign antigens (McGettigan et al., 2001a (McGettigan et al., , 2001b Schnell et al., 2000) . To evaluate the cell surface expression of the recombinant RV G-D4 fusion proteins, BSR cells were infected with recombinant vaccinia virus expressing T7 RNA polymerase for one h, transfected with 5 μg of each of the five plasmids and 24 h later fixed in paraformaldehyde, permeabilized, and immunostained with anti-PA monoclonal antibody. In contrast, PA expressed on the surface of virus particles induced potent humoral responses with either live or killed SPBN-D4-E51 particles stimulating antibody production after only a single dose.	./cache/cord-340983-w219g6qs.txt	./txt/cord-340983-w219g6qs.txt