id	sid	tid	token	lemma	pos
cord-253024-b393ea2u	1	1	key	key	NNP
cord-253024-b393ea2u	1	2	:	:	:
cord-253024-b393ea2u	1	3	cord-253024-b393ea2u	cord-253024-b393ea2u	NNP
cord-253024-b393ea2u	2	1	authors	author	NNS
cord-253024-b393ea2u	2	2	:	:	:
cord-253024-b393ea2u	2	3	Fu	Fu	NNP
cord-253024-b393ea2u	2	4	,	,	,
cord-253024-b393ea2u	2	5	Kaisong	Kaisong	NNP
cord-253024-b393ea2u	2	6	;	;	:
cord-253024-b393ea2u	3	1	Baric	Baric	NNP
cord-253024-b393ea2u	3	2	,	,	,
cord-253024-b393ea2u	3	3	Ralph	Ralph	NNP
cord-253024-b393ea2u	3	4	S.	S.	NNP
cord-253024-b393ea2u	3	5	title	title	NN
cord-253024-b393ea2u	3	6	:	:	:
cord-253024-b393ea2u	3	7	Evidence	evidence	NN
cord-253024-b393ea2u	3	8	for	for	IN
cord-253024-b393ea2u	3	9	variable	variable	JJ
cord-253024-b393ea2u	3	10	rates	rate	NNS
cord-253024-b393ea2u	3	11	of	of	IN
cord-253024-b393ea2u	3	12	recombination	recombination	NN
cord-253024-b393ea2u	3	13	in	in	IN
cord-253024-b393ea2u	3	14	the	the	DT
cord-253024-b393ea2u	3	15	MHV	MHV	NNP
cord-253024-b393ea2u	3	16	genome	genome	NN
cord-253024-b393ea2u	3	17	date	date	NN
cord-253024-b393ea2u	3	18	:	:	:
cord-253024-b393ea2u	3	19	1992	1992	CD
cord-253024-b393ea2u	3	20	-	-	HYPH
cord-253024-b393ea2u	3	21	07	07	CD
cord-253024-b393ea2u	3	22	-	-	HYPH
cord-253024-b393ea2u	3	23	31	31	CD
cord-253024-b393ea2u	3	24	journal	journal	NN
cord-253024-b393ea2u	3	25	:	:	:
cord-253024-b393ea2u	4	1	Virology	virology	NN
cord-253024-b393ea2u	4	2	DOI	DOI	NNP
cord-253024-b393ea2u	4	3	:	:	:
cord-253024-b393ea2u	4	4	10.1016/0042	10.1016/0042	CD
cord-253024-b393ea2u	4	5	-	-	SYM
cord-253024-b393ea2u	4	6	6822(92)90684-h	6822(92)90684-h	CD
cord-253024-b393ea2u	4	7	sha	sha	NN
cord-253024-b393ea2u	4	8	:	:	:
cord-253024-b393ea2u	5	1	6d60088295d3d6ae75adb805206f8844ba5c1288	6d60088295d3d6ae75adb805206f8844ba5c1288	CD
cord-253024-b393ea2u	5	2	doc_id	doc_id	CD
cord-253024-b393ea2u	5	3	:	:	:
cord-253024-b393ea2u	6	1	253024	253024	CD
cord-253024-b393ea2u	6	2	cord_uid	cord_uid	NNS
cord-253024-b393ea2u	6	3	:	:	:
cord-253024-b393ea2u	7	1	b393ea2u	b393ea2u	NFP
cord-253024-b393ea2u	7	2	Abstract	Abstract	NNP
cord-253024-b393ea2u	7	3	Mouse	Mouse	NNP
cord-253024-b393ea2u	7	4	hepatitis	hepatitis	NN
cord-253024-b393ea2u	7	5	virus	virus	NN
cord-253024-b393ea2u	7	6	has	have	VBZ
cord-253024-b393ea2u	7	7	been	be	VBN
cord-253024-b393ea2u	7	8	shown	show	VBN
cord-253024-b393ea2u	7	9	to	to	TO
cord-253024-b393ea2u	7	10	undergo	undergo	VB
cord-253024-b393ea2u	7	11	RNA	RNA	NNP
cord-253024-b393ea2u	7	12	recombination	recombination	NN
cord-253024-b393ea2u	7	13	at	at	IN
cord-253024-b393ea2u	7	14	high	high	JJ
cord-253024-b393ea2u	7	15	frequency	frequency	NN
cord-253024-b393ea2u	7	16	during	during	IN
cord-253024-b393ea2u	7	17	mixed	mixed	JJ
cord-253024-b393ea2u	7	18	infection	infection	NN
cord-253024-b393ea2u	7	19	.	.	.
cord-253024-b393ea2u	8	1	Temperature	temperature	NN
cord-253024-b393ea2u	8	2	-	-	HYPH
cord-253024-b393ea2u	8	3	sensitive	sensitive	JJ
cord-253024-b393ea2u	8	4	mutants	mutant	NNS
cord-253024-b393ea2u	8	5	were	be	VBD
cord-253024-b393ea2u	8	6	isolated	isolate	VBN
cord-253024-b393ea2u	8	7	using	use	VBG
cord-253024-b393ea2u	8	8	5-fluorouracil	5-fluorouracil	CD
cord-253024-b393ea2u	8	9	and	and	CC
cord-253024-b393ea2u	8	10	5-azacytidine	5-azacytidine	CD
cord-253024-b393ea2u	8	11	as	as	IN
cord-253024-b393ea2u	8	12	mutagen	mutagen	NN
cord-253024-b393ea2u	8	13	.	.	.
cord-253024-b393ea2u	9	1	Six	six	CD
cord-253024-b393ea2u	9	2	RNA+	RNA+	NNP
cord-253024-b393ea2u	9	3	mutants	mutant	NNS
cord-253024-b393ea2u	9	4	that	that	WDT
cord-253024-b393ea2u	9	5	reside	reside	VBP
cord-253024-b393ea2u	9	6	within	within	IN
cord-253024-b393ea2u	9	7	a	a	DT
cord-253024-b393ea2u	9	8	single	single	JJ
cord-253024-b393ea2u	9	9	complementation	complementation	NN
cord-253024-b393ea2u	9	10	group	group	NN
cord-253024-b393ea2u	9	11	mapping	mapping	NN
cord-253024-b393ea2u	9	12	within	within	IN
cord-253024-b393ea2u	9	13	the	the	DT
cord-253024-b393ea2u	9	14	S	S	NNP
cord-253024-b393ea2u	9	15	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	9	16	gene	gene	NN
cord-253024-b393ea2u	9	17	of	of	IN
cord-253024-b393ea2u	9	18	MHV	MHV	NNP
cord-253024-b393ea2u	9	19	-	-	HYPH
cord-253024-b393ea2u	9	20	A59	A59	NNP
cord-253024-b393ea2u	9	21	were	be	VBD
cord-253024-b393ea2u	9	22	isolated	isolate	VBN
cord-253024-b393ea2u	9	23	which	which	WDT
cord-253024-b393ea2u	9	24	did	do	VBD
cord-253024-b393ea2u	9	25	not	not	RB
cord-253024-b393ea2u	9	26	cause	cause	VB
cord-253024-b393ea2u	9	27	syncytium	syncytium	NN
cord-253024-b393ea2u	9	28	at	at	IN
cord-253024-b393ea2u	9	29	the	the	DT
cord-253024-b393ea2u	9	30	restrictive	restrictive	JJ
cord-253024-b393ea2u	9	31	temperature	temperature	NN
cord-253024-b393ea2u	9	32	.	.	.
cord-253024-b393ea2u	10	1	Using	use	VBG
cord-253024-b393ea2u	10	2	standard	standard	JJ
cord-253024-b393ea2u	10	3	genetic	genetic	JJ
cord-253024-b393ea2u	10	4	techniques	technique	NNS
cord-253024-b393ea2u	10	5	,	,	,
cord-253024-b393ea2u	10	6	a	a	DT
cord-253024-b393ea2u	10	7	recombination	recombination	NN
cord-253024-b393ea2u	10	8	map	map	NN
cord-253024-b393ea2u	10	9	was	be	VBD
cord-253024-b393ea2u	10	10	established	establish	VBN
cord-253024-b393ea2u	10	11	that	that	WDT
cord-253024-b393ea2u	10	12	indicated	indicate	VBD
cord-253024-b393ea2u	10	13	that	that	IN
cord-253024-b393ea2u	10	14	these	these	DT
cord-253024-b393ea2u	10	15	mutants	mutant	NNS
cord-253024-b393ea2u	10	16	mapped	map	VBD
cord-253024-b393ea2u	10	17	into	into	IN
cord-253024-b393ea2u	10	18	two	two	CD
cord-253024-b393ea2u	10	19	distinct	distinct	JJ
cord-253024-b393ea2u	10	20	domains	domain	NNS
cord-253024-b393ea2u	10	21	designated	designate	VBN
cord-253024-b393ea2u	10	22	F1	F1	NNP
cord-253024-b393ea2u	10	23	and	and	CC
cord-253024-b393ea2u	10	24	F2	f2	NN
cord-253024-b393ea2u	10	25	.	.	.
cord-253024-b393ea2u	11	1	These	these	DT
cord-253024-b393ea2u	11	2	genetic	genetic	JJ
cord-253024-b393ea2u	11	3	domains	domain	NNS
cord-253024-b393ea2u	11	4	may	may	MD
cord-253024-b393ea2u	11	5	correspond	correspond	VB
cord-253024-b393ea2u	11	6	to	to	IN
cord-253024-b393ea2u	11	7	mutations	mutation	NNS
cord-253024-b393ea2u	11	8	mapping	mapping	NN
cord-253024-b393ea2u	11	9	within	within	IN
cord-253024-b393ea2u	11	10	the	the	DT
cord-253024-b393ea2u	11	11	S1	S1	NNP
cord-253024-b393ea2u	11	12	and	and	CC
cord-253024-b393ea2u	11	13	S2	s2	NN
cord-253024-b393ea2u	11	14	glycoproteins	glycoprotein	NNS
cord-253024-b393ea2u	11	15	,	,	,
cord-253024-b393ea2u	11	16	respectively	respectively	RB
cord-253024-b393ea2u	11	17	,	,	,
cord-253024-b393ea2u	11	18	and	and	CC
cord-253024-b393ea2u	11	19	suggest	suggest	VBP
cord-253024-b393ea2u	11	20	that	that	IN
cord-253024-b393ea2u	11	21	both	both	PDT
cord-253024-b393ea2u	11	22	the	the	DT
cord-253024-b393ea2u	11	23	S1	S1	NNP
cord-253024-b393ea2u	11	24	and	and	CC
cord-253024-b393ea2u	11	25	S2	s2	NN
cord-253024-b393ea2u	11	26	domains	domain	NNS
cord-253024-b393ea2u	11	27	are	be	VBP
cord-253024-b393ea2u	11	28	important	important	JJ
cord-253024-b393ea2u	11	29	in	in	IN
cord-253024-b393ea2u	11	30	eliciting	elicit	VBG
cord-253024-b393ea2u	11	31	the	the	DT
cord-253024-b393ea2u	11	32	fusogenic	fusogenic	JJ
cord-253024-b393ea2u	11	33	activity	activity	NN
cord-253024-b393ea2u	11	34	of	of	IN
cord-253024-b393ea2u	11	35	the	the	DT
cord-253024-b393ea2u	11	36	S	S	NNP
cord-253024-b393ea2u	11	37	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	11	38	gene	gene	NN
cord-253024-b393ea2u	11	39	.	.	.
cord-253024-b393ea2u	12	1	In	in	IN
cord-253024-b393ea2u	12	2	addition	addition	NN
cord-253024-b393ea2u	12	3	,	,	,
cord-253024-b393ea2u	12	4	assuming	assume	VBG
cord-253024-b393ea2u	12	5	that	that	IN
cord-253024-b393ea2u	12	6	most	most	RBS
cord-253024-b393ea2u	12	7	distal	distal	JJ
cord-253024-b393ea2u	12	8	is	be	VBZ
cord-253024-b393ea2u	12	9	alleles	allele	NNS
cord-253024-b393ea2u	12	10	map	map	VBP
cord-253024-b393ea2u	12	11	roughly	roughly	RB
cord-253024-b393ea2u	12	12	4.0	4.0	CD
cord-253024-b393ea2u	12	13	kb	kb	NNS
cord-253024-b393ea2u	12	14	apart	apart	RB
cord-253024-b393ea2u	12	15	,	,	,
cord-253024-b393ea2u	12	16	a	a	DT
cord-253024-b393ea2u	12	17	recombination	recombination	NN
cord-253024-b393ea2u	12	18	frequency	frequency	NN
cord-253024-b393ea2u	12	19	of	of	IN
cord-253024-b393ea2u	12	20	1	1	CD
cord-253024-b393ea2u	12	21	%	%	NN
cord-253024-b393ea2u	12	22	per	per	IN
cord-253024-b393ea2u	12	23	575–676	575–676	CD
cord-253024-b393ea2u	12	24	by	by	IN
cord-253024-b393ea2u	12	25	was	be	VBD
cord-253024-b393ea2u	12	26	predicted	predict	VBN
cord-253024-b393ea2u	12	27	through	through	IN
cord-253024-b393ea2u	12	28	the	the	DT
cord-253024-b393ea2u	12	29	S	S	NNP
cord-253024-b393ea2u	12	30	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	12	31	gene	gene	NN
cord-253024-b393ea2u	12	32	.	.	.
cord-253024-b393ea2u	13	1	Interestingly	interestingly	RB
cord-253024-b393ea2u	13	2	,	,	,
cord-253024-b393ea2u	13	3	this	this	DT
cord-253024-b393ea2u	13	4	represents	represent	VBZ
cord-253024-b393ea2u	13	5	a	a	DT
cord-253024-b393ea2u	13	6	threefold	threefold	JJ
cord-253024-b393ea2u	13	7	increase	increase	NN
cord-253024-b393ea2u	13	8	in	in	IN
cord-253024-b393ea2u	13	9	the	the	DT
cord-253024-b393ea2u	13	10	recombination	recombination	NN
cord-253024-b393ea2u	13	11	frequency	frequency	NN
cord-253024-b393ea2u	13	12	as	as	IN
cord-253024-b393ea2u	13	13	compared	compare	VBN
cord-253024-b393ea2u	13	14	to	to	IN
cord-253024-b393ea2u	13	15	rates	rate	NNS
cord-253024-b393ea2u	13	16	predicted	predict	VBN
cord-253024-b393ea2u	13	17	through	through	IN
cord-253024-b393ea2u	13	18	the	the	DT
cord-253024-b393ea2u	13	19	polymerase	polymerase	NN
cord-253024-b393ea2u	13	20	region	region	NN
cord-253024-b393ea2u	13	21	.	.	.
cord-253024-b393ea2u	14	1	The	the	DT
cord-253024-b393ea2u	14	2	increase	increase	NN
cord-253024-b393ea2u	14	3	in	in	IN
cord-253024-b393ea2u	14	4	the	the	DT
cord-253024-b393ea2u	14	5	recombination	recombination	NN
cord-253024-b393ea2u	14	6	rate	rate	NN
cord-253024-b393ea2u	14	7	was	be	VBD
cord-253024-b393ea2u	14	8	probably	probably	RB
cord-253024-b393ea2u	14	9	not	not	RB
cord-253024-b393ea2u	14	10	due	due	JJ
cord-253024-b393ea2u	14	11	to	to	IN
cord-253024-b393ea2u	14	12	recombination	recombination	NN
cord-253024-b393ea2u	14	13	events	event	NNS
cord-253024-b393ea2u	14	14	resulting	result	VBG
cord-253024-b393ea2u	14	15	in	in	IN
cord-253024-b393ea2u	14	16	large	large	JJ
cord-253024-b393ea2u	14	17	deletions	deletion	NNS
cord-253024-b393ea2u	14	18	or	or	CC
cord-253024-b393ea2u	14	19	insertions	insertion	NNS
cord-253024-b393ea2u	14	20	(	(	-LRB-
cord-253024-b393ea2u	14	21	>	>	NFP
cord-253024-b393ea2u	14	22	50	50	CD
cord-253024-b393ea2u	14	23	bp	bp	NNP
cord-253024-b393ea2u	14	24	)	)	-RRB-
cord-253024-b393ea2u	14	25	,	,	,
cord-253024-b393ea2u	14	26	but	but	CC
cord-253024-b393ea2u	14	27	rather	rather	RB
cord-253024-b393ea2u	14	28	was	be	VBD
cord-253024-b393ea2u	14	29	probably	probably	RB
cord-253024-b393ea2u	14	30	due	due	JJ
cord-253024-b393ea2u	14	31	to	to	IN
cord-253024-b393ea2u	14	32	a	a	DT
cord-253024-b393ea2u	14	33	combination	combination	NN
cord-253024-b393ea2u	14	34	of	of	IN
cord-253024-b393ea2u	14	35	homologous	homologous	JJ
cord-253024-b393ea2u	14	36	and	and	CC
cord-253024-b393ea2u	14	37	nonhomologous	nonhomologous	JJ
cord-253024-b393ea2u	14	38	recombination	recombination	NN
cord-253024-b393ea2u	14	39	.	.	.
cord-253024-b393ea2u	15	1	A	a	DT
cord-253024-b393ea2u	15	2	variety	variety	NN
cord-253024-b393ea2u	15	3	of	of	IN
cord-253024-b393ea2u	15	4	explanations	explanation	NNS
cord-253024-b393ea2u	15	5	could	could	MD
cord-253024-b393ea2u	15	6	account	account	VB
cord-253024-b393ea2u	15	7	for	for	IN
cord-253024-b393ea2u	15	8	the	the	DT
cord-253024-b393ea2u	15	9	increased	increase	VBN
cord-253024-b393ea2u	15	10	rates	rate	NNS
cord-253024-b393ea2u	15	11	of	of	IN
cord-253024-b393ea2u	15	12	recombination	recombination	NN
cord-253024-b393ea2u	15	13	in	in	IN
cord-253024-b393ea2u	15	14	the	the	DT
cord-253024-b393ea2u	15	15	S	S	NNP
cord-253024-b393ea2u	15	16	gene	gene	NN
cord-253024-b393ea2u	15	17	.	.	.
cord-253024-b393ea2u	16	1	Mouse	Mouse	NNP
cord-253024-b393ea2u	16	2	hepatitis	hepatitis	NN
cord-253024-b393ea2u	16	3	virus	virus	NN
cord-253024-b393ea2u	16	4	(	(	-LRB-
cord-253024-b393ea2u	16	5	MHV	MHV	NNP
cord-253024-b393ea2u	16	6	)	)	-RRB-
cord-253024-b393ea2u	16	7	,	,	,
cord-253024-b393ea2u	16	8	a	a	DT
cord-253024-b393ea2u	16	9	member	member	NN
cord-253024-b393ea2u	16	10	of	of	IN
cord-253024-b393ea2u	16	11	Coronaviridae	Coronaviridae	NNP
cord-253024-b393ea2u	16	12	,	,	,
cord-253024-b393ea2u	16	13	contains	contain	VBZ
cord-253024-b393ea2u	16	14	a	a	DT
cord-253024-b393ea2u	16	15	single	single	JJ
cord-253024-b393ea2u	16	16	-	-	HYPH
cord-253024-b393ea2u	16	17	stranded	strand	VBN
cord-253024-b393ea2u	16	18	nonsegmented	nonsegmented	JJ
cord-253024-b393ea2u	16	19	plus	plus	NN
cord-253024-b393ea2u	16	20	-	-	HYPH
cord-253024-b393ea2u	16	21	polarity	polarity	NN
cord-253024-b393ea2u	16	22	RNA	RNA	NNP
cord-253024-b393ea2u	16	23	of	of	IN
cord-253024-b393ea2u	16	24	about	about	RB
cord-253024-b393ea2u	16	25	32	32	CD
cord-253024-b393ea2u	16	26	kb	kb	NNS
cord-253024-b393ea2u	16	27	in	in	IN
cord-253024-b393ea2u	16	28	length	length	NN
cord-253024-b393ea2u	17	1	(	(	-LRB-
cord-253024-b393ea2u	17	2	Lee	Lee	NNP
cord-253024-b393ea2u	17	3	et	et	NNP
cord-253024-b393ea2u	17	4	a/.	a/.	NNP
cord-253024-b393ea2u	17	5	,	,	,
cord-253024-b393ea2u	17	6	1991	1991	CD
cord-253024-b393ea2u	17	7	;	;	:
cord-253024-b393ea2u	17	8	Pachuk	Pachuk	NNP
cord-253024-b393ea2u	17	9	et	et	NNP
cord-253024-b393ea2u	17	10	a/.	a/.	NN
cord-253024-b393ea2u	17	11	,	,	,
cord-253024-b393ea2u	17	12	1989	1989	CD
cord-253024-b393ea2u	17	13	)	)	-RRB-
cord-253024-b393ea2u	17	14	.	.	.
cord-253024-b393ea2u	18	1	The	the	DT
cord-253024-b393ea2u	18	2	genomic	genomic	JJ
cord-253024-b393ea2u	18	3	RNA	rna	NN
cord-253024-b393ea2u	18	4	is	be	VBZ
cord-253024-b393ea2u	18	5	arranged	arrange	VBN
cord-253024-b393ea2u	18	6	into	into	IN
cord-253024-b393ea2u	18	7	seven	seven	CD
cord-253024-b393ea2u	18	8	or	or	CC
cord-253024-b393ea2u	18	9	eight	eight	CD
cord-253024-b393ea2u	18	10	coding	code	VBG
cord-253024-b393ea2u	18	11	regions	region	NNS
cord-253024-b393ea2u	18	12	and	and	CC
cord-253024-b393ea2u	18	13	is	be	VBZ
cord-253024-b393ea2u	18	14	encapsidated	encapsidate	VBN
cord-253024-b393ea2u	18	15	within	within	IN
cord-253024-b393ea2u	18	16	multiple	multiple	JJ
cord-253024-b393ea2u	18	17	copies	copy	NNS
cord-253024-b393ea2u	18	18	of	of	IN
cord-253024-b393ea2u	18	19	a	a	DT
cord-253024-b393ea2u	18	20	50-kDa	50-kda	CD
cord-253024-b393ea2u	18	21	nucleocapsid	nucleocapsid	NN
cord-253024-b393ea2u	18	22	protein	protein	NN
cord-253024-b393ea2u	18	23	(	(	-LRB-
cord-253024-b393ea2u	18	24	N	N	NNP
cord-253024-b393ea2u	18	25	)	)	-RRB-
cord-253024-b393ea2u	18	26	(	(	-LRB-
cord-253024-b393ea2u	18	27	Lai	Lai	NNP
cord-253024-b393ea2u	18	28	,	,	,
cord-253024-b393ea2u	18	29	1990	1990	CD
cord-253024-b393ea2u	18	30	;	;	:
cord-253024-b393ea2u	18	31	Siddell	Siddell	NNP
cord-253024-b393ea2u	18	32	,	,	,
cord-253024-b393ea2u	18	33	1983	1983	CD
cord-253024-b393ea2u	18	34	)	)	-RRB-
cord-253024-b393ea2u	18	35	.	.	.
cord-253024-b393ea2u	19	1	The	the	DT
cord-253024-b393ea2u	19	2	N	N	NNP
cord-253024-b393ea2u	19	3	protein	protein	NN
cord-253024-b393ea2u	19	4	forms	form	VBZ
cord-253024-b393ea2u	19	5	a	a	DT
cord-253024-b393ea2u	19	6	helical	helical	JJ
cord-253024-b393ea2u	19	7	nucleocapsid	nucleocapsid	NN
cord-253024-b393ea2u	19	8	structure	structure	NN
cord-253024-b393ea2u	19	9	that	that	WDT
cord-253024-b393ea2u	19	10	is	be	VBZ
cord-253024-b393ea2u	19	11	probably	probably	RB
cord-253024-b393ea2u	19	12	associated	associate	VBN
cord-253024-b393ea2u	19	13	with	with	IN
cord-253024-b393ea2u	19	14	the	the	DT
cord-253024-b393ea2u	19	15	transcriptional	transcriptional	JJ
cord-253024-b393ea2u	19	16	complex	complex	NN
cord-253024-b393ea2u	19	17	Compton	Compton	NNP
cord-253024-b393ea2u	19	18	et	et	NNP
cord-253024-b393ea2u	19	19	a/.	a/.	NNP
cord-253024-b393ea2u	19	20	,	,	,
cord-253024-b393ea2u	19	21	1987	1987	CD
cord-253024-b393ea2u	19	22	;	;	:
cord-253024-b393ea2u	19	23	Stohlman	Stohlman	NNP
cord-253024-b393ea2u	19	24	and	and	CC
cord-253024-b393ea2u	19	25	Lai	Lai	NNP
cord-253024-b393ea2u	19	26	,	,	,
cord-253024-b393ea2u	19	27	1979	1979	CD
cord-253024-b393ea2u	19	28	;	;	:
cord-253024-b393ea2u	19	29	Stohlman	Stohlman	NNP
cord-253024-b393ea2u	19	30	eta/.	eta/.	NNP
cord-253024-b393ea2u	19	31	,	,	,
cord-253024-b393ea2u	19	32	1988	1988	CD
cord-253024-b393ea2u	19	33	;	;	:
cord-253024-b393ea2u	20	1	Sturman	Sturman	NNP
cord-253024-b393ea2u	20	2	eta/.	eta/.	CD
cord-253024-b393ea2u	20	3	,	,	,
cord-253024-b393ea2u	20	4	1980	1980	CD
cord-253024-b393ea2u	20	5	)	)	-RRB-
cord-253024-b393ea2u	20	6	.	.	.
cord-253024-b393ea2u	21	1	The	the	DT
cord-253024-b393ea2u	21	2	nucleocapsid	nucleocapsid	NN
cord-253024-b393ea2u	21	3	structure	structure	NN
cord-253024-b393ea2u	21	4	is	be	VBZ
cord-253024-b393ea2u	21	5	surrounded	surround	VBN
cord-253024-b393ea2u	21	6	by	by	IN
cord-253024-b393ea2u	21	7	a	a	DT
cord-253024-b393ea2u	21	8	lipid	lipid	NN
cord-253024-b393ea2u	21	9	envelope	envelope	NN
cord-253024-b393ea2u	21	10	and	and	CC
cord-253024-b393ea2u	21	11	contains	contain	VBZ
cord-253024-b393ea2u	21	12	two	two	CD
cord-253024-b393ea2u	21	13	or	or	CC
cord-253024-b393ea2u	21	14	three	three	CD
cord-253024-b393ea2u	21	15	virus	virus	NN
cord-253024-b393ea2u	21	16	-	-	HYPH
cord-253024-b393ea2u	21	17	specific	specific	JJ
cord-253024-b393ea2u	21	18	glycoproteins	glycoprotein	NNS
cord-253024-b393ea2u	21	19	.	.	.
cord-253024-b393ea2u	22	1	The	the	DT
cord-253024-b393ea2u	22	2	S	S	NNP
cord-253024-b393ea2u	22	3	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	22	4	has	have	VBZ
cord-253024-b393ea2u	22	5	a	a	DT
cord-253024-b393ea2u	22	6	molecular	molecular	JJ
cord-253024-b393ea2u	22	7	weight	weight	NN
cord-253024-b393ea2u	22	8	of	of	IN
cord-253024-b393ea2u	22	9	180	180	CD
cord-253024-b393ea2u	22	10	kDa	kDa	NNS
cord-253024-b393ea2u	22	11	and	and	CC
cord-253024-b393ea2u	22	12	is	be	VBZ
cord-253024-b393ea2u	22	13	frequently	frequently	RB
cord-253024-b393ea2u	22	14	cleaved	cleave	VBN
cord-253024-b393ea2u	22	15	into	into	IN
cord-253024-b393ea2u	22	16	two	two	CD
cord-253024-b393ea2u	22	17	90-kDa	90-kda	CD
cord-253024-b393ea2u	22	18	glycoproteins	glycoprotein	NNS
cord-253024-b393ea2u	22	19	designated	designate	VBN
cord-253024-b393ea2u	22	20	Sl	Sl	NNP
cord-253024-b393ea2u	22	21	and	and	CC
cord-253024-b393ea2u	22	22	S2	S2	NNP
cord-253024-b393ea2u	22	23	(	(	-LRB-
cord-253024-b393ea2u	22	24	Frana	Frana	NNP
cord-253024-b393ea2u	22	25	et	et	NNP
cord-253024-b393ea2u	22	26	a/.	a/.	NN
cord-253024-b393ea2u	22	27	,	,	,
cord-253024-b393ea2u	22	28	1985	1985	CD
cord-253024-b393ea2u	22	29	;	;	:
cord-253024-b393ea2u	22	30	Sturman	Sturman	NNP
cord-253024-b393ea2u	22	31	et	et	NNP
cord-253024-b393ea2u	22	32	a/.	a/.	NNP
cord-253024-b393ea2u	22	33	,	,	,
cord-253024-b393ea2u	22	34	1980	1980	CD
cord-253024-b393ea2u	22	35	.	.	.
cord-253024-b393ea2u	23	1	The	the	DT
cord-253024-b393ea2u	23	2	N	n	CD
cord-253024-b393ea2u	23	3	-	-	HYPH
cord-253024-b393ea2u	23	4	terminal	terminal	JJ
cord-253024-b393ea2u	23	5	signal	signal	NN
cord-253024-b393ea2u	23	6	sequence	sequence	NN
cord-253024-b393ea2u	23	7	is	be	VBZ
cord-253024-b393ea2u	23	8	contained	contain	VBN
cord-253024-b393ea2u	23	9	within	within	IN
cord-253024-b393ea2u	23	10	the	the	DT
cord-253024-b393ea2u	23	11	Sl	sl	NN
cord-253024-b393ea2u	23	12	domain	domain	NN
cord-253024-b393ea2u	23	13	while	while	IN
cord-253024-b393ea2u	23	14	the	the	DT
cord-253024-b393ea2u	23	15	C	C	NNP
cord-253024-b393ea2u	23	16	-	-	HYPH
cord-253024-b393ea2u	23	17	terminus	terminus	NN
cord-253024-b393ea2u	23	18	is	be	VBZ
cord-253024-b393ea2u	23	19	contained	contain	VBN
cord-253024-b393ea2u	23	20	within	within	IN
cord-253024-b393ea2u	23	21	the	the	DT
cord-253024-b393ea2u	23	22	S2	S2	NNP
cord-253024-b393ea2u	23	23	region	region	NN
cord-253024-b393ea2u	23	24	(	(	-LRB-
cord-253024-b393ea2u	23	25	Boireau	Boireau	NNP
cord-253024-b393ea2u	23	26	et	et	NNP
cord-253024-b393ea2u	23	27	al	al	NNP
cord-253024-b393ea2u	23	28	.	.	NNP
cord-253024-b393ea2u	23	29	,	,	,
cord-253024-b393ea2u	23	30	1990	1990	CD
cord-253024-b393ea2u	23	31	;	;	:
cord-253024-b393ea2u	23	32	Luytjes	Luytjes	NNP
cord-253024-b393ea2u	23	33	eta/.	eta/.	CD
cord-253024-b393ea2u	23	34	,	,	,
cord-253024-b393ea2u	23	35	1987	1987	CD
cord-253024-b393ea2u	23	36	;	;	:
cord-253024-b393ea2u	23	37	Rasschaert	Rasschaert	NNP
cord-253024-b393ea2u	23	38	et	et	NNP
cord-253024-b393ea2u	23	39	al	al	NNP
cord-253024-b393ea2u	23	40	.	.	NNP
cord-253024-b393ea2u	23	41	,	,	,
cord-253024-b393ea2u	23	42	1990	1990	CD
cord-253024-b393ea2u	23	43	;	;	:
cord-253024-b393ea2u	23	44	Schmidt	Schmidt	NNP
cord-253024-b393ea2u	23	45	et	et	NNP
cord-253024-b393ea2u	23	46	al	al	NNP
cord-253024-b393ea2u	23	47	.	.	NNP
cord-253024-b393ea2u	23	48	,	,	,
cord-253024-b393ea2u	23	49	1987	1987	CD
cord-253024-b393ea2u	23	50	)	)	-RRB-
cord-253024-b393ea2u	23	51	.	.	.
cord-253024-b393ea2u	24	1	The	the	DT
cord-253024-b393ea2u	24	2	Sl	sl	NN
cord-253024-b393ea2u	24	3	domain	domain	NN
cord-253024-b393ea2u	24	4	can	can	MD
cord-253024-b393ea2u	24	5	undergo	undergo	VB
cord-253024-b393ea2u	24	6	significant	significant	JJ
cord-253024-b393ea2u	24	7	amino	amino	NN
cord-253024-b393ea2u	24	8	acid	acid	NN
cord-253024-b393ea2u	24	9	alteration	alteration	NN
cord-253024-b393ea2u	24	10	without	without	IN
cord-253024-b393ea2u	24	11	losing	lose	VBG
cord-253024-b393ea2u	24	12	its	-PRON-	PRP$
cord-253024-b393ea2u	24	13	function	function	NN
cord-253024-b393ea2u	24	14	while	while	IN
cord-253024-b393ea2u	24	15	the	the	DT
cord-253024-b393ea2u	24	16	S2	S2	NNP
cord-253024-b393ea2u	24	17	domain	domain	NN
cord-253024-b393ea2u	24	18	is	be	VBZ
cord-253024-b393ea2u	24	19	more	more	RBR
cord-253024-b393ea2u	24	20	highly	highly	RB
cord-253024-b393ea2u	24	21	conserved	conserve	VBN
cord-253024-b393ea2u	24	22	and	and	CC
cord-253024-b393ea2u	24	23	contains	contain	VBZ
cord-253024-b393ea2u	24	24	a	a	DT
cord-253024-b393ea2u	24	25	hydrophobic	hydrophobic	JJ
cord-253024-b393ea2u	24	26	heptad	heptad	NN
cord-253024-b393ea2u	24	27	repeat	repeat	NN
cord-253024-b393ea2u	24	28	element	element	NN
cord-253024-b393ea2u	24	29	that	that	WDT
cord-253024-b393ea2u	24	30	probably	probably	RB
cord-253024-b393ea2u	24	31	forms	form	VBZ
cord-253024-b393ea2u	24	32	a	a	DT
cord-253024-b393ea2u	24	33	complex	complex	NN
cord-253024-b393ea2u	24	34	'	'	''
cord-253024-b393ea2u	24	35	To	to	IN
cord-253024-b393ea2u	24	36	whom	whom	WP
cord-253024-b393ea2u	24	37	correspondence	correspondence	NN
cord-253024-b393ea2u	24	38	should	should	MD
cord-253024-b393ea2u	24	39	be	be	VB
cord-253024-b393ea2u	24	40	addressed	address	VBN
cord-253024-b393ea2u	24	41	.	.	.
cord-253024-b393ea2u	25	1	coiled	coiled	JJ
cord-253024-b393ea2u	25	2	coil	coil	NN
cord-253024-b393ea2u	25	3	(	(	-LRB-
cord-253024-b393ea2u	25	4	Luytjes	Luytjes	NNP
cord-253024-b393ea2u	25	5	et	et	NNP
cord-253024-b393ea2u	25	6	a/.	a/.	NN
cord-253024-b393ea2u	25	7	,	,	,
cord-253024-b393ea2u	25	8	1987	1987	CD
cord-253024-b393ea2u	25	9	;	;	:
cord-253024-b393ea2u	25	10	Schmidt	Schmidt	NNP
cord-253024-b393ea2u	25	11	et	et	NNP
cord-253024-b393ea2u	25	12	a/.	a/.	NNP
cord-253024-b393ea2u	25	13	,	,	,
cord-253024-b393ea2u	25	14	1987	1987	CD
cord-253024-b393ea2u	25	15	)	)	-RRB-
cord-253024-b393ea2u	25	16	.	.	.
cord-253024-b393ea2u	26	1	The	the	DT
cord-253024-b393ea2u	26	2	S	S	NNP
cord-253024-b393ea2u	26	3	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	26	4	is	be	VBZ
cord-253024-b393ea2u	26	5	responsible	responsible	JJ
cord-253024-b393ea2u	26	6	for	for	IN
cord-253024-b393ea2u	26	7	virus	virus	NN
cord-253024-b393ea2u	26	8	binding	bind	VBG
cord-253024-b393ea2u	26	9	to	to	IN
cord-253024-b393ea2u	26	10	the	the	DT
cord-253024-b393ea2u	26	11	specific	specific	JJ
cord-253024-b393ea2u	26	12	cellular	cellular	JJ
cord-253024-b393ea2u	26	13	receptor	receptor	NN
cord-253024-b393ea2u	26	14	,	,	,
cord-253024-b393ea2u	26	15	induction	induction	NN
cord-253024-b393ea2u	26	16	of	of	IN
cord-253024-b393ea2u	26	17	cell	cell	NN
cord-253024-b393ea2u	26	18	fusion	fusion	NN
cord-253024-b393ea2u	26	19	,	,	,
cord-253024-b393ea2u	26	20	and	and	CC
cord-253024-b393ea2u	26	21	elicitation	elicitation	NN
cord-253024-b393ea2u	26	22	of	of	IN
cord-253024-b393ea2u	26	23	neutralizing	neutralize	VBG
cord-253024-b393ea2u	26	24	antibody	antibody	NN
cord-253024-b393ea2u	26	25	and	and	CC
cord-253024-b393ea2u	26	26	cell	cell	NN
cord-253024-b393ea2u	26	27	-	-	HYPH
cord-253024-b393ea2u	26	28	mediated	mediate	VBN
cord-253024-b393ea2u	26	29	immunity	immunity	NN
cord-253024-b393ea2u	26	30	(	(	-LRB-
cord-253024-b393ea2u	26	31	Collins	Collins	NNP
cord-253024-b393ea2u	26	32	et	et	NNP
cord-253024-b393ea2u	26	33	al	al	NNP
cord-253024-b393ea2u	26	34	.	.	.
cord-253024-b393ea2u	27	1	,	,	,
cord-253024-b393ea2u	27	2	1982	1982	CD
cord-253024-b393ea2u	27	3	;	;	:
cord-253024-b393ea2u	27	4	Fleming	Fleming	NNP
cord-253024-b393ea2u	27	5	et	et	NNP
cord-253024-b393ea2u	27	6	a/.	a/.	NNP
cord-253024-b393ea2u	27	7	,	,	,
cord-253024-b393ea2u	27	8	1986	1986	CD
cord-253024-b393ea2u	27	9	;	;	:
cord-253024-b393ea2u	27	10	Sturman	Sturman	NNP
cord-253024-b393ea2u	27	11	et	et	NNP
cord-253024-b393ea2u	27	12	a/.	a/.	NNP
cord-253024-b393ea2u	27	13	,	,	,
cord-253024-b393ea2u	27	14	1980	1980	CD
cord-253024-b393ea2u	27	15	.	.	.
cord-253024-b393ea2u	28	1	In	in	IN
cord-253024-b393ea2u	28	2	addition	addition	NN
cord-253024-b393ea2u	28	3	to	to	IN
cord-253024-b393ea2u	28	4	S	S	NNP
cord-253024-b393ea2u	28	5	,	,	,
cord-253024-b393ea2u	28	6	a	a	DT
cord-253024-b393ea2u	28	7	23-kDa	23-kda	CD
cord-253024-b393ea2u	28	8	M	M	NNP
cord-253024-b393ea2u	28	9	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	28	10	is	be	VBZ
cord-253024-b393ea2u	28	11	present	present	JJ
cord-253024-b393ea2u	28	12	in	in	IN
cord-253024-b393ea2u	28	13	the	the	DT
cord-253024-b393ea2u	28	14	virion	virion	NN
cord-253024-b393ea2u	28	15	that	that	WDT
cord-253024-b393ea2u	28	16	probably	probably	RB
cord-253024-b393ea2u	28	17	functions	function	VBZ
cord-253024-b393ea2u	28	18	in	in	IN
cord-253024-b393ea2u	28	19	assembly	assembly	NN
cord-253024-b393ea2u	28	20	and	and	CC
cord-253024-b393ea2u	28	21	release	release	NN
cord-253024-b393ea2u	28	22	(	(	-LRB-
cord-253024-b393ea2u	28	23	Sturman	Sturman	NNP
cord-253024-b393ea2u	28	24	et	et	NNP
cord-253024-b393ea2u	28	25	a/.	a/.	NNP
cord-253024-b393ea2u	28	26	,	,	,
cord-253024-b393ea2u	28	27	1980	1980	CD
cord-253024-b393ea2u	28	28	.	.	.
cord-253024-b393ea2u	29	1	In	in	IN
cord-253024-b393ea2u	29	2	some	some	DT
cord-253024-b393ea2u	29	3	strains	strain	NNS
cord-253024-b393ea2u	29	4	of	of	IN
cord-253024-b393ea2u	29	5	MHV	MHV	NNP
cord-253024-b393ea2u	29	6	,	,	,
cord-253024-b393ea2u	29	7	a	a	DT
cord-253024-b393ea2u	29	8	65-kDa	65-kda	CD
cord-253024-b393ea2u	29	9	hemagglutinin	hemagglutinin	NN
cord-253024-b393ea2u	29	10	(	(	-LRB-
cord-253024-b393ea2u	29	11	HE	HE	NNP
cord-253024-b393ea2u	29	12	)	)	-RRB-
cord-253024-b393ea2u	29	13	that	that	IN
cord-253024-b393ea2u	29	14	shares	share	VBZ
cord-253024-b393ea2u	29	15	considerable	considerable	JJ
cord-253024-b393ea2u	29	16	sequence	sequence	NN
cord-253024-b393ea2u	29	17	homology	homology	NN
cord-253024-b393ea2u	29	18	with	with	IN
cord-253024-b393ea2u	29	19	the	the	DT
cord-253024-b393ea2u	29	20	influenza	influenza	NN
cord-253024-b393ea2u	29	21	C	C	NNP
cord-253024-b393ea2u	29	22	virus	virus	NN
cord-253024-b393ea2u	29	23	hemagglutinin	hemagglutinin	NN
cord-253024-b393ea2u	29	24	is	be	VBZ
cord-253024-b393ea2u	29	25	also	also	RB
cord-253024-b393ea2u	29	26	present	present	JJ
cord-253024-b393ea2u	29	27	(	(	-LRB-
cord-253024-b393ea2u	29	28	Luytjes	Luytjes	NNP
cord-253024-b393ea2u	29	29	et	et	NNP
cord-253024-b393ea2u	29	30	al	al	NNP
cord-253024-b393ea2u	29	31	.	.	NNP
cord-253024-b393ea2u	29	32	,	,	,
cord-253024-b393ea2u	29	33	1988	1988	CD
cord-253024-b393ea2u	30	1	;	;	:
cord-253024-b393ea2u	30	2	Shieh	Shieh	NNP
cord-253024-b393ea2u	30	3	et	et	NNP
cord-253024-b393ea2u	30	4	al	al	NNP
cord-253024-b393ea2u	30	5	.	.	NNP
cord-253024-b393ea2u	30	6	,	,	,
cord-253024-b393ea2u	30	7	1989	1989	CD
cord-253024-b393ea2u	30	8	;	;	:
cord-253024-b393ea2u	30	9	Yokomori	Yokomori	NNP
cord-253024-b393ea2u	30	10	et	et	NNP
cord-253024-b393ea2u	30	11	al	al	NNP
cord-253024-b393ea2u	30	12	.	.	NNP
cord-253024-b393ea2u	30	13	,	,	,
cord-253024-b393ea2u	30	14	1989	1989	CD
cord-253024-b393ea2u	30	15	)	)	-RRB-
cord-253024-b393ea2u	30	16	.	.	.
cord-253024-b393ea2u	31	1	Upon	upon	IN
cord-253024-b393ea2u	31	2	entry	entry	NN
cord-253024-b393ea2u	31	3	into	into	IN
cord-253024-b393ea2u	31	4	susceptible	susceptible	JJ
cord-253024-b393ea2u	31	5	cells	cell	NNS
cord-253024-b393ea2u	31	6	,	,	,
cord-253024-b393ea2u	31	7	the	the	DT
cord-253024-b393ea2u	31	8	genomic	genomic	JJ
cord-253024-b393ea2u	31	9	RNA	RNA	NNP
cord-253024-b393ea2u	31	10	is	be	VBZ
cord-253024-b393ea2u	31	11	translated	translate	VBN
cord-253024-b393ea2u	31	12	into	into	IN
cord-253024-b393ea2u	31	13	one	one	CD
cord-253024-b393ea2u	31	14	or	or	CC
cord-253024-b393ea2u	31	15	more	more	JJR
cord-253024-b393ea2u	31	16	polyproteins	polyprotein	NNS
cord-253024-b393ea2u	31	17	that	that	WDT
cord-253024-b393ea2u	31	18	act	act	VBP
cord-253024-b393ea2u	31	19	as	as	IN
cord-253024-b393ea2u	31	20	an	an	DT
cord-253024-b393ea2u	31	21	RNA	RNA	NNP
cord-253024-b393ea2u	31	22	dependent	dependent	JJ
cord-253024-b393ea2u	31	23	RNA	RNA	NNP
cord-253024-b393ea2u	31	24	polymerase	polymerase	NN
cord-253024-b393ea2u	31	25	(	(	-LRB-
cord-253024-b393ea2u	31	26	Mahy	Mahy	NNP
cord-253024-b393ea2u	31	27	et	et	FW
cord-253024-b393ea2u	31	28	a/.	a/.	NN
cord-253024-b393ea2u	31	29	,	,	,
cord-253024-b393ea2u	31	30	1983	1983	CD
cord-253024-b393ea2u	31	31	;	;	:
cord-253024-b393ea2u	31	32	Brayton	Brayton	NNP
cord-253024-b393ea2u	31	33	et	et	NNP
cord-253024-b393ea2u	31	34	al	al	NNP
cord-253024-b393ea2u	31	35	.	.	NNP
cord-253024-b393ea2u	31	36	,	,	,
cord-253024-b393ea2u	31	37	1982	1982	CD
cord-253024-b393ea2u	32	1	Brayton	Brayton	NNP
cord-253024-b393ea2u	32	2	et	et	NNP
cord-253024-b393ea2u	32	3	al	al	NNP
cord-253024-b393ea2u	32	4	.	.	NNP
cord-253024-b393ea2u	32	5	,	,	,
cord-253024-b393ea2u	32	6	,	,	,
cord-253024-b393ea2u	32	7	1984	1984	CD
cord-253024-b393ea2u	32	8	.	.	.
cord-253024-b393ea2u	33	1	While	while	IN
cord-253024-b393ea2u	33	2	the	the	DT
cord-253024-b393ea2u	33	3	exact	exact	JJ
cord-253024-b393ea2u	33	4	mechanism	mechanism	NN
cord-253024-b393ea2u	33	5	for	for	IN
cord-253024-b393ea2u	33	6	MHV	MHV	NNP
cord-253024-b393ea2u	33	7	transcription	transcription	NN
cord-253024-b393ea2u	33	8	is	be	VBZ
cord-253024-b393ea2u	33	9	still	still	RB
cord-253024-b393ea2u	33	10	under	under	IN
cord-253024-b393ea2u	33	11	study	study	NN
cord-253024-b393ea2u	33	12	,	,	,
cord-253024-b393ea2u	33	13	the	the	DT
cord-253024-b393ea2u	33	14	preponderance	preponderance	NN
cord-253024-b393ea2u	33	15	of	of	IN
cord-253024-b393ea2u	33	16	data	datum	NNS
cord-253024-b393ea2u	33	17	suggest	suggest	VBP
cord-253024-b393ea2u	33	18	that	that	IN
cord-253024-b393ea2u	33	19	the	the	DT
cord-253024-b393ea2u	33	20	genome	genome	NN
cord-253024-b393ea2u	33	21	is	be	VBZ
cord-253024-b393ea2u	33	22	initially	initially	RB
cord-253024-b393ea2u	33	23	transcribed	transcribe	VBN
cord-253024-b393ea2u	33	24	into	into	IN
cord-253024-b393ea2u	33	25	a	a	DT
cord-253024-b393ea2u	33	26	full	full	JJ
cord-253024-b393ea2u	33	27	-	-	HYPH
cord-253024-b393ea2u	33	28	length	length	NN
cord-253024-b393ea2u	33	29	minus	minus	CC
cord-253024-b393ea2u	33	30	strand	strand	NN
cord-253024-b393ea2u	33	31	RNA	RNA	NNP
cord-253024-b393ea2u	33	32	,	,	,
cord-253024-b393ea2u	33	33	which	which	WDT
cord-253024-b393ea2u	33	34	acts	act	VBZ
cord-253024-b393ea2u	33	35	as	as	IN
cord-253024-b393ea2u	33	36	template	template	NN
cord-253024-b393ea2u	33	37	for	for	IN
cord-253024-b393ea2u	33	38	the	the	DT
cord-253024-b393ea2u	33	39	synthesis	synthesis	NN
cord-253024-b393ea2u	33	40	of	of	IN
cord-253024-b393ea2u	33	41	a	a	DT
cord-253024-b393ea2u	33	42	genomelength	genomelength	NNP
cord-253024-b393ea2u	33	43	RNA	RNA	NNP
cord-253024-b393ea2u	33	44	and	and	CC
cord-253024-b393ea2u	33	45	six	six	CD
cord-253024-b393ea2u	33	46	subgenomic	subgenomic	JJ
cord-253024-b393ea2u	33	47	mRNAs	mRNAs	NNPS
cord-253024-b393ea2u	33	48	by	by	IN
cord-253024-b393ea2u	33	49	"	"	``
cord-253024-b393ea2u	33	50	leaderprimed	leaderprimed	JJ
cord-253024-b393ea2u	33	51	"	"	''
cord-253024-b393ea2u	33	52	transcription	transcription	NN
cord-253024-b393ea2u	33	53	(	(	-LRB-
cord-253024-b393ea2u	33	54	Baker	Baker	NNP
cord-253024-b393ea2u	33	55	et	et	NNP
cord-253024-b393ea2u	33	56	a/.	a/.	NNP
cord-253024-b393ea2u	33	57	,	,	,
cord-253024-b393ea2u	33	58	1990	1990	CD
cord-253024-b393ea2u	33	59	;	;	:
cord-253024-b393ea2u	33	60	Baric	Baric	NNP
cord-253024-b393ea2u	33	61	et	et	NNP
cord-253024-b393ea2u	33	62	al	al	NNP
cord-253024-b393ea2u	33	63	.	.	NNP
cord-253024-b393ea2u	33	64	,	,	,
cord-253024-b393ea2u	33	65	1983	1983	CD
cord-253024-b393ea2u	33	66	Baric	Baric	NNP
cord-253024-b393ea2u	33	67	et	et	NNP
cord-253024-b393ea2u	33	68	al	al	NNP
cord-253024-b393ea2u	33	69	.	.	NNP
cord-253024-b393ea2u	33	70	,	,	,
cord-253024-b393ea2u	33	71	,	,	,
cord-253024-b393ea2u	33	72	1985	1985	CD
cord-253024-b393ea2u	33	73	Makino	Makino	NNP
cord-253024-b393ea2u	33	74	et	et	NNP
cord-253024-b393ea2u	33	75	a/.	a/.	NN
cord-253024-b393ea2u	33	76	,	,	,
cord-253024-b393ea2u	33	77	1986a	1986a	CD
cord-253024-b393ea2u	33	78	Makino	Makino	NNP
cord-253024-b393ea2u	33	79	et	et	NNP
cord-253024-b393ea2u	33	80	a/.	a/.	NN
cord-253024-b393ea2u	33	81	,	,	,
cord-253024-b393ea2u	33	82	,	,	,
cord-253024-b393ea2u	33	83	1991	1991	CD
cord-253024-b393ea2u	33	84	.	.	.
cord-253024-b393ea2u	34	1	In	in	IN
cord-253024-b393ea2u	34	2	turn	turn	NN
cord-253024-b393ea2u	34	3	,	,	,
cord-253024-b393ea2u	34	4	the	the	DT
cord-253024-b393ea2u	34	5	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	34	6	mRNA	mRNA	NNP
cord-253024-b393ea2u	34	7	then	then	RB
cord-253024-b393ea2u	34	8	act	act	VB
cord-253024-b393ea2u	34	9	as	as	IN
cord-253024-b393ea2u	34	10	template	template	NN
cord-253024-b393ea2u	34	11	for	for	IN
cord-253024-b393ea2u	34	12	the	the	DT
cord-253024-b393ea2u	34	13	synthesis	synthesis	NN
cord-253024-b393ea2u	34	14	of	of	IN
cord-253024-b393ea2u	34	15	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	34	16	minus	minus	CC
cord-253024-b393ea2u	34	17	strands	strand	NNS
cord-253024-b393ea2u	34	18	,	,	,
cord-253024-b393ea2u	34	19	which	which	WDT
cord-253024-b393ea2u	34	20	participate	participate	VBP
cord-253024-b393ea2u	34	21	in	in	IN
cord-253024-b393ea2u	34	22	successive	successive	JJ
cord-253024-b393ea2u	34	23	rounds	round	NNS
cord-253024-b393ea2u	34	24	of	of	IN
cord-253024-b393ea2u	34	25	mRNA	mrna	NN
cord-253024-b393ea2u	34	26	amplification	amplification	NN
cord-253024-b393ea2u	34	27	(	(	-LRB-
cord-253024-b393ea2u	34	28	Sawicki	Sawicki	NNP
cord-253024-b393ea2u	34	29	and	and	CC
cord-253024-b393ea2u	34	30	Sawicki	Sawicki	NNP
cord-253024-b393ea2u	34	31	,	,	,
cord-253024-b393ea2u	34	32	1990	1990	CD
cord-253024-b393ea2u	34	33	;	;	:
cord-253024-b393ea2u	34	34	Sethna	Sethna	NNP
cord-253024-b393ea2u	34	35	et	et	NNP
cord-253024-b393ea2u	34	36	a/.	a/.	NNP
cord-253024-b393ea2u	34	37	,	,	,
cord-253024-b393ea2u	34	38	1989	1989	CD
cord-253024-b393ea2u	34	39	Sethna	Sethna	NNP
cord-253024-b393ea2u	34	40	et	et	NNP
cord-253024-b393ea2u	34	41	a/.	a/.	NNP
cord-253024-b393ea2u	34	42	,	,	,
cord-253024-b393ea2u	34	43	,	,	,
cord-253024-b393ea2u	34	44	1991	1991	CD
cord-253024-b393ea2u	34	45	.	.	.
cord-253024-b393ea2u	35	1	Homologous	homologous	JJ
cord-253024-b393ea2u	35	2	recombination	recombination	NN
cord-253024-b393ea2u	35	3	among	among	IN
cord-253024-b393ea2u	35	4	viruses	virus	NNS
cord-253024-b393ea2u	35	5	with	with	IN
cord-253024-b393ea2u	35	6	nonsegmented	nonsegmente	VBN
cord-253024-b393ea2u	35	7	RNA	RNA	NNP
cord-253024-b393ea2u	35	8	genomes	genome	NNS
cord-253024-b393ea2u	35	9	has	have	VBZ
cord-253024-b393ea2u	35	10	been	be	VBN
cord-253024-b393ea2u	35	11	reported	report	VBN
cord-253024-b393ea2u	35	12	among	among	IN
cord-253024-b393ea2u	35	13	picornaviruses	picornavirus	NNS
cord-253024-b393ea2u	35	14	(	(	-LRB-
cord-253024-b393ea2u	35	15	Cooper	Cooper	NNP
cord-253024-b393ea2u	35	16	,	,	,
cord-253024-b393ea2u	35	17	1968	1968	CD
cord-253024-b393ea2u	35	18	(	(	-LRB-
cord-253024-b393ea2u	35	19	Cooper	Cooper	NNP
cord-253024-b393ea2u	35	20	,	,	,
cord-253024-b393ea2u	35	21	,	,	,
cord-253024-b393ea2u	35	22	1977	1977	CD
cord-253024-b393ea2u	35	23	Cooper	Cooper	NNP
cord-253024-b393ea2u	35	24	et	et	NNP
cord-253024-b393ea2u	35	25	al	al	NNP
cord-253024-b393ea2u	35	26	.	.	NNP
cord-253024-b393ea2u	35	27	,	,	,
cord-253024-b393ea2u	35	28	1975	1975	CD
cord-253024-b393ea2u	35	29	;	;	:
cord-253024-b393ea2u	35	30	King	King	NNP
cord-253024-b393ea2u	35	31	et	et	NNP
cord-253024-b393ea2u	35	32	al	al	NNP
cord-253024-b393ea2u	35	33	.	.	NNP
cord-253024-b393ea2u	35	34	,	,	,
cord-253024-b393ea2u	36	1	1982	1982	CD
cord-253024-b393ea2u	37	1	King	King	NNP
cord-253024-b393ea2u	37	2	et	et	NNP
cord-253024-b393ea2u	37	3	al	al	NNP
cord-253024-b393ea2u	37	4	.	.	.
cord-253024-b393ea2u	38	1	,	,	,
cord-253024-b393ea2u	38	2	,	,	,
cord-253024-b393ea2u	38	3	1985	1985	CD
cord-253024-b393ea2u	39	1	King	King	NNP
cord-253024-b393ea2u	39	2	et	et	NNP
cord-253024-b393ea2u	39	3	al	al	NNP
cord-253024-b393ea2u	39	4	.	.	NNP
cord-253024-b393ea2u	39	5	,	,	,
cord-253024-b393ea2u	39	6	,	,	,
cord-253024-b393ea2u	39	7	1987	1987	CD
cord-253024-b393ea2u	40	1	Lake	Lake	NNP
cord-253024-b393ea2u	40	2	et	et	NNP
cord-253024-b393ea2u	40	3	al	al	NNP
cord-253024-b393ea2u	40	4	.	.	NNP
cord-253024-b393ea2u	40	5	,	,	,
cord-253024-b393ea2u	40	6	1975	1975	CD
cord-253024-b393ea2u	40	7	)	)	-RRB-
cord-253024-b393ea2u	40	8	,	,	,
cord-253024-b393ea2u	40	9	brome	brome	NNP
cord-253024-b393ea2u	40	10	mosaic	mosaic	NNP
cord-253024-b393ea2u	40	11	virus	virus	NN
cord-253024-b393ea2u	40	12	(	(	-LRB-
cord-253024-b393ea2u	40	13	Bujarski	Bujarski	NNP
cord-253024-b393ea2u	40	14	and	and	CC
cord-253024-b393ea2u	40	15	Kaesberg	Kaesberg	NNP
cord-253024-b393ea2u	40	16	,	,	,
cord-253024-b393ea2u	40	17	1986	1986	CD
cord-253024-b393ea2u	40	18	)	)	-RRB-
cord-253024-b393ea2u	40	19	alphaviruses	alphaviruses	NNP
cord-253024-b393ea2u	40	20	(	(	-LRB-
cord-253024-b393ea2u	41	1	Hahn	Hahn	NNP
cord-253024-b393ea2u	41	2	et	et	NNP
cord-253024-b393ea2u	41	3	al	al	NNP
cord-253024-b393ea2u	41	4	.	.	.
cord-253024-b393ea2u	42	1	,	,	,
cord-253024-b393ea2u	42	2	1988	1988	CD
cord-253024-b393ea2u	42	3	;	;	:
cord-253024-b393ea2u	42	4	Weiss	Weiss	NNP
cord-253024-b393ea2u	42	5	and	and	CC
cord-253024-b393ea2u	42	6	Schlesinger	Schlesinger	NNP
cord-253024-b393ea2u	42	7	,	,	,
cord-253024-b393ea2u	42	8	1991	1991	CD
cord-253024-b393ea2u	42	9	)	)	-RRB-
cord-253024-b393ea2u	42	10	,	,	,
cord-253024-b393ea2u	42	11	cowpea	cowpea	NNP
cord-253024-b393ea2u	42	12	chlorotic	chlorotic	JJ
cord-253024-b393ea2u	42	13	mottle	mottle	NN
cord-253024-b393ea2u	42	14	virus	virus	NN
cord-253024-b393ea2u	42	15	3a	3a	CD
cord-253024-b393ea2u	42	16	(	(	-LRB-
cord-253024-b393ea2u	42	17	Allison	Allison	NNP
cord-253024-b393ea2u	42	18	et	et	NNP
cord-253024-b393ea2u	42	19	al	al	NNP
cord-253024-b393ea2u	42	20	.	.	NNP
cord-253024-b393ea2u	42	21	,	,	,
cord-253024-b393ea2u	42	22	1990	1990	CD
cord-253024-b393ea2u	42	23	)	)	-RRB-
cord-253024-b393ea2u	42	24	,	,	,
cord-253024-b393ea2u	42	25	and	and	CC
cord-253024-b393ea2u	42	26	coronaviruses	coronaviruse	NNS
cord-253024-b393ea2u	42	27	(	(	-LRB-
cord-253024-b393ea2u	42	28	Keck	Keck	NNP
cord-253024-b393ea2u	42	29	et	et	NNP
cord-253024-b393ea2u	42	30	al	al	NNP
cord-253024-b393ea2u	42	31	.	.	NNP
cord-253024-b393ea2u	42	32	,	,	,
cord-253024-b393ea2u	42	33	1988a	1988a	CD
cord-253024-b393ea2u	42	34	,	,	,
cord-253024-b393ea2u	42	35	b	b	LS
cord-253024-b393ea2u	42	36	;	;	:
cord-253024-b393ea2u	42	37	Lai	Lai	NNP
cord-253024-b393ea2u	42	38	et	et	NNP
cord-253024-b393ea2u	42	39	a/.	a/.	NN
cord-253024-b393ea2u	42	40	,	,	,
cord-253024-b393ea2u	42	41	1985	1985	CD
cord-253024-b393ea2u	42	42	;	;	:
cord-253024-b393ea2u	43	1	Makino	Makino	NNP
cord-253024-b393ea2u	43	2	et	et	NNP
cord-253024-b393ea2u	43	3	al	al	NNP
cord-253024-b393ea2u	43	4	.	.	NNP
cord-253024-b393ea2u	43	5	,	,	,
cord-253024-b393ea2u	43	6	1986b	1986b	CD
cord-253024-b393ea2u	43	7	)	)	-RRB-
cord-253024-b393ea2u	43	8	.	.	.
cord-253024-b393ea2u	44	1	During	during	IN
cord-253024-b393ea2u	44	2	MHV	MHV	NNP
cord-253024-b393ea2u	44	3	replication	replication	NN
cord-253024-b393ea2u	44	4	,	,	,
cord-253024-b393ea2u	44	5	the	the	DT
cord-253024-b393ea2u	44	6	RNA	RNA	NNP
cord-253024-b393ea2u	44	7	recombination	recombination	NN
cord-253024-b393ea2u	44	8	frequency	frequency	NN
cord-253024-b393ea2u	44	9	is	be	VBZ
cord-253024-b393ea2u	44	10	unusually	unusually	RB
cord-253024-b393ea2u	44	11	high	high	JJ
cord-253024-b393ea2u	44	12	and	and	CC
cord-253024-b393ea2u	44	13	approaches	approach	VBZ
cord-253024-b393ea2u	44	14	25	25	CD
cord-253024-b393ea2u	44	15	%	%	NN
cord-253024-b393ea2u	44	16	or	or	CC
cord-253024-b393ea2u	44	17	more	more	JJR
cord-253024-b393ea2u	44	18	during	during	IN
cord-253024-b393ea2u	44	19	mixed	mixed	JJ
cord-253024-b393ea2u	44	20	infection	infection	NN
cord-253024-b393ea2u	44	21	.	.	.
cord-253024-b393ea2u	45	1	Recently	recently	RB
cord-253024-b393ea2u	45	2	,	,	,
cord-253024-b393ea2u	45	3	biochemical	biochemical	JJ
cord-253024-b393ea2u	45	4	analysis	analysis	NN
cord-253024-b393ea2u	45	5	has	have	VBZ
cord-253024-b393ea2u	45	6	revealed	reveal	VBN
cord-253024-b393ea2u	45	7	extensive	extensive	JJ
cord-253024-b393ea2u	45	8	polymorphisms	polymorphism	NNS
cord-253024-b393ea2u	45	9	and	and	CC
cord-253024-b393ea2u	45	10	deletions	deletion	NNS
cord-253024-b393ea2u	45	11	in	in	IN
cord-253024-b393ea2u	45	12	the	the	DT
cord-253024-b393ea2u	45	13	sequence	sequence	NN
cord-253024-b393ea2u	45	14	of	of	IN
cord-253024-b393ea2u	45	15	the	the	DT
cord-253024-b393ea2u	45	16	S	S	NNP
cord-253024-b393ea2u	45	17	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	45	18	gene	gene	NN
cord-253024-b393ea2u	45	19	of	of	IN
cord-253024-b393ea2u	45	20	different	different	JJ
cord-253024-b393ea2u	45	21	MHV	MHV	NNP
cord-253024-b393ea2u	45	22	variants	variant	NNS
cord-253024-b393ea2u	45	23	.	.	.
cord-253024-b393ea2u	46	1	The	the	DT
cord-253024-b393ea2u	46	2	variant	variant	NN
cord-253024-b393ea2u	46	3	viruses	virus	NNS
cord-253024-b393ea2u	46	4	were	be	VBD
cord-253024-b393ea2u	46	5	probably	probably	RB
cord-253024-b393ea2u	46	6	derived	derive	VBN
cord-253024-b393ea2u	46	7	from	from	IN
cord-253024-b393ea2u	46	8	nonhomologous	nonhomologous	JJ
cord-253024-b393ea2u	46	9	recombination	recombination	NN
cord-253024-b393ea2u	46	10	because	because	IN
cord-253024-b393ea2u	46	11	no	no	DT
cord-253024-b393ea2u	46	12	consensus	consensus	NN
cord-253024-b393ea2u	46	13	or	or	CC
cord-253024-b393ea2u	46	14	conserved	conserved	JJ
cord-253024-b393ea2u	46	15	sequences	sequence	NNS
cord-253024-b393ea2u	46	16	flanked	flank	VBD
cord-253024-b393ea2u	46	17	the	the	DT
cord-253024-b393ea2u	46	18	deletions	deletion	NNS
cord-253024-b393ea2u	46	19	(	(	-LRB-
cord-253024-b393ea2u	46	20	Banner	Banner	NNP
cord-253024-b393ea2u	46	21	et	et	FW
cord-253024-b393ea2u	46	22	al	al	NNP
cord-253024-b393ea2u	46	23	.	.	NNP
cord-253024-b393ea2u	46	24	,	,	,
cord-253024-b393ea2u	46	25	1990	1990	CD
cord-253024-b393ea2u	46	26	;	;	:
cord-253024-b393ea2u	46	27	Parker	Parker	NNP
cord-253024-b393ea2u	46	28	et	et	NNP
cord-253024-b393ea2u	46	29	a/.	a/.	NNP
cord-253024-b393ea2u	46	30	,	,	,
cord-253024-b393ea2u	46	31	1990	1990	CD
cord-253024-b393ea2u	46	32	)	)	-RRB-
cord-253024-b393ea2u	46	33	.	.	.
cord-253024-b393ea2u	47	1	Sequence	sequence	NN
cord-253024-b393ea2u	47	2	analysis	analysis	NN
cord-253024-b393ea2u	47	3	of	of	IN
cord-253024-b393ea2u	47	4	RNA	RNA	NNP
cord-253024-b393ea2u	47	5	recombinant	recombinant	JJ
cord-253024-b393ea2u	47	6	viruses	virus	NNS
cord-253024-b393ea2u	47	7	also	also	RB
cord-253024-b393ea2u	47	8	suggests	suggest	VBZ
cord-253024-b393ea2u	47	9	the	the	DT
cord-253024-b393ea2u	47	10	presence	presence	NN
cord-253024-b393ea2u	47	11	of	of	IN
cord-253024-b393ea2u	47	12	a	a	DT
cord-253024-b393ea2u	47	13	recombination	recombination	NN
cord-253024-b393ea2u	47	14	"	"	``
cord-253024-b393ea2u	47	15	hot	hot	JJ
cord-253024-b393ea2u	47	16	spot	spot	NN
cord-253024-b393ea2u	47	17	"	"	''
cord-253024-b393ea2u	47	18	in	in	IN
cord-253024-b393ea2u	47	19	the	the	DT
cord-253024-b393ea2u	47	20	MHV	MHV	NNP
cord-253024-b393ea2u	47	21	S	S	NNP
cord-253024-b393ea2u	47	22	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	47	23	gene	gene	NN
cord-253024-b393ea2u	47	24	(	(	-LRB-
cord-253024-b393ea2u	47	25	Banner	Banner	NNP
cord-253024-b393ea2u	47	26	el	el	NNP
cord-253024-b393ea2u	47	27	al	al	NNP
cord-253024-b393ea2u	47	28	.	.	NNP
cord-253024-b393ea2u	47	29	,	,	,
cord-253024-b393ea2u	47	30	1990	1990	CD
cord-253024-b393ea2u	47	31	)	)	-RRB-
cord-253024-b393ea2u	47	32	.	.	.
cord-253024-b393ea2u	48	1	However	however	RB
cord-253024-b393ea2u	48	2	,	,	,
cord-253024-b393ea2u	48	3	in	in	IN
cord-253024-b393ea2u	48	4	the	the	DT
cord-253024-b393ea2u	48	5	absence	absence	NN
cord-253024-b393ea2u	48	6	of	of	IN
cord-253024-b393ea2u	48	7	selection	selection	NN
cord-253024-b393ea2u	48	8	pressure	pressure	NN
cord-253024-b393ea2u	48	9	,	,	,
cord-253024-b393ea2u	48	10	RNA	rna	NN
cord-253024-b393ea2u	48	11	recombination	recombination	NN
cord-253024-b393ea2u	48	12	sites	site	NNS
cord-253024-b393ea2u	48	13	in	in	IN
cord-253024-b393ea2u	48	14	this	this	DT
cord-253024-b393ea2u	48	15	region	region	NN
cord-253024-b393ea2u	48	16	are	be	VBP
cord-253024-b393ea2u	48	17	random	random	JJ
cord-253024-b393ea2u	48	18	,	,	,
cord-253024-b393ea2u	48	19	suggesting	suggest	VBG
cord-253024-b393ea2u	48	20	that	that	IN
cord-253024-b393ea2u	48	21	this	this	DT
cord-253024-b393ea2u	48	22	preferred	preferred	JJ
cord-253024-b393ea2u	48	23	site	site	NN
cord-253024-b393ea2u	48	24	reflects	reflect	VBZ
cord-253024-b393ea2u	48	25	the	the	DT
cord-253024-b393ea2u	48	26	selection	selection	NN
cord-253024-b393ea2u	48	27	for	for	IN
cord-253024-b393ea2u	48	28	biologically	biologically	RB
cord-253024-b393ea2u	48	29	more	more	RBR
cord-253024-b393ea2u	48	30	efficient	efficient	JJ
cord-253024-b393ea2u	48	31	recombinants	recombinant	NNS
cord-253024-b393ea2u	48	32	.	.	.
cord-253024-b393ea2u	49	1	Recombination	recombination	NN
cord-253024-b393ea2u	49	2	analysis	analysis	NN
cord-253024-b393ea2u	49	3	provides	provide	VBZ
cord-253024-b393ea2u	49	4	a	a	DT
cord-253024-b393ea2u	49	5	powerful	powerful	JJ
cord-253024-b393ea2u	49	6	tool	tool	NN
cord-253024-b393ea2u	49	7	for	for	IN
cord-253024-b393ea2u	49	8	mapping	map	VBG
cord-253024-b393ea2u	49	9	genetic	genetic	JJ
cord-253024-b393ea2u	49	10	loci	locus	NNS
cord-253024-b393ea2u	49	11	and	and	CC
cord-253024-b393ea2u	49	12	determining	determine	VBG
cord-253024-b393ea2u	49	13	the	the	DT
cord-253024-b393ea2u	49	14	recombination	recombination	NN
cord-253024-b393ea2u	49	15	frequency	frequency	NN
cord-253024-b393ea2u	49	16	between	between	IN
cord-253024-b393ea2u	49	17	individual	individual	JJ
cord-253024-b393ea2u	49	18	temperature	temperature	NN
cord-253024-b393ea2u	49	19	sensitive	sensitive	JJ
cord-253024-b393ea2u	49	20	(	(	-LRB-
cord-253024-b393ea2u	49	21	ts	ts	NNP
cord-253024-b393ea2u	49	22	)	)	-RRB-
cord-253024-b393ea2u	49	23	mutations	mutation	NNS
cord-253024-b393ea2u	49	24	Cooper	Cooper	NNP
cord-253024-b393ea2u	49	25	,	,	,
cord-253024-b393ea2u	49	26	1968	1968	CD
cord-253024-b393ea2u	49	27	Cooper	Cooper	NNP
cord-253024-b393ea2u	49	28	,	,	,
cord-253024-b393ea2u	49	29	,	,	,
cord-253024-b393ea2u	49	30	1977	1977	CD
cord-253024-b393ea2u	50	1	Lake	Lake	NNP
cord-253024-b393ea2u	50	2	et	et	NNP
cord-253024-b393ea2u	50	3	al	al	NNP
cord-253024-b393ea2u	50	4	.	.	NNP
cord-253024-b393ea2u	50	5	,	,	,
cord-253024-b393ea2u	50	6	1975	1975	CD
cord-253024-b393ea2u	50	7	)	)	-RRB-
cord-253024-b393ea2u	50	8	.	.	.
cord-253024-b393ea2u	51	1	In	in	IN
cord-253024-b393ea2u	51	2	this	this	DT
cord-253024-b393ea2u	51	3	study	study	NN
cord-253024-b393ea2u	51	4	,	,	,
cord-253024-b393ea2u	51	5	several	several	JJ
cord-253024-b393ea2u	51	6	group	group	NN
cord-253024-b393ea2u	51	7	F	F	NNP
cord-253024-b393ea2u	51	8	RNA+	RNA+	NNP
cord-253024-b393ea2u	51	9	ts	ts	NN
cord-253024-b393ea2u	51	10	mutants	mutant	NNS
cord-253024-b393ea2u	51	11	were	be	VBD
cord-253024-b393ea2u	51	12	used	use	VBN
cord-253024-b393ea2u	51	13	to	to	TO
cord-253024-b393ea2u	51	14	establish	establish	VB
cord-253024-b393ea2u	51	15	a	a	DT
cord-253024-b393ea2u	51	16	genetic	genetic	JJ
cord-253024-b393ea2u	51	17	recombination	recombination	NN
cord-253024-b393ea2u	51	18	map	map	NN
cord-253024-b393ea2u	51	19	in	in	IN
cord-253024-b393ea2u	51	20	the	the	DT
cord-253024-b393ea2u	51	21	S	S	NNP
cord-253024-b393ea2u	51	22	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	51	23	gene	gene	NN
cord-253024-b393ea2u	51	24	of	of	IN
cord-253024-b393ea2u	51	25	MHV	MHV	NNP
cord-253024-b393ea2u	51	26	-	-	HYPH
cord-253024-b393ea2u	51	27	A59	A59	NNP
cord-253024-b393ea2u	51	28	.	.	.
cord-253024-b393ea2u	52	1	Temperature	temperature	NN
cord-253024-b393ea2u	52	2	-	-	HYPH
cord-253024-b393ea2u	52	3	sensitive	sensitive	JJ
cord-253024-b393ea2u	52	4	mutants	mutant	NNS
cord-253024-b393ea2u	52	5	representing	represent	VBG
cord-253024-b393ea2u	52	6	the	the	DT
cord-253024-b393ea2u	52	7	group	group	NN
cord-253024-b393ea2u	52	8	F	F	NNP
cord-253024-b393ea2u	52	9	RNA+	RNA+	NNP
cord-253024-b393ea2u	52	10	mutants	mutant	NNS
cord-253024-b393ea2u	52	11	(	(	-LRB-
cord-253024-b393ea2u	52	12	LA7	LA7	NNP
cord-253024-b393ea2u	52	13	,	,	,
cord-253024-b393ea2u	52	14	LA1	LA1	NNP
cord-253024-b393ea2u	52	15	2	2	CD
cord-253024-b393ea2u	52	16	,	,	,
cord-253024-b393ea2u	52	17	NC6	NC6	NNP
cord-253024-b393ea2u	52	18	,	,	,
cord-253024-b393ea2u	52	19	NC1	NC1	NNP
cord-253024-b393ea2u	52	20	4	4	CD
cord-253024-b393ea2u	52	21	,	,	,
cord-253024-b393ea2u	52	22	NC1	NC1	NNP
cord-253024-b393ea2u	52	23	6	6	CD
cord-253024-b393ea2u	52	24	,	,	,
cord-253024-b393ea2u	52	25	NC1	NC1	NNP
cord-253024-b393ea2u	52	26	7	7	CD
cord-253024-b393ea2u	52	27	)	)	-RRB-
cord-253024-b393ea2u	52	28	and	and	CC
cord-253024-b393ea2u	52	29	group	group	NN
cord-253024-b393ea2u	52	30	E	E	NNP
cord-253024-b393ea2u	52	31	RNA	RNA	NNP
cord-253024-b393ea2u	52	32	-	-	HYPH
cord-253024-b393ea2u	52	33	mutants	mutant	NNS
cord-253024-b393ea2u	52	34	(	(	-LRB-
cord-253024-b393ea2u	52	35	LA1	LA1	NNP
cord-253024-b393ea2u	52	36	8)	8)	NN
cord-253024-b393ea2u	52	37	of	of	IN
cord-253024-b393ea2u	52	38	MHVA59	MHVA59	NNP
cord-253024-b393ea2u	52	39	were	be	VBD
cord-253024-b393ea2u	52	40	used	use	VBN
cord-253024-b393ea2u	52	41	throughout	throughout	IN
cord-253024-b393ea2u	52	42	the	the	DT
cord-253024-b393ea2u	52	43	course	course	NN
cord-253024-b393ea2u	52	44	of	of	IN
cord-253024-b393ea2u	52	45	this	this	DT
cord-253024-b393ea2u	52	46	study	study	NN
cord-253024-b393ea2u	52	47	.	.	.
cord-253024-b393ea2u	53	1	All	all	DT
cord-253024-b393ea2u	53	2	virus	virus	NN
cord-253024-b393ea2u	53	3	stocks	stock	NNS
cord-253024-b393ea2u	53	4	were	be	VBD
cord-253024-b393ea2u	53	5	propagated	propagate	VBN
cord-253024-b393ea2u	53	6	at	at	IN
cord-253024-b393ea2u	53	7	32	32	CD
cord-253024-b393ea2u	53	8	"	"	``
cord-253024-b393ea2u	53	9	in	in	IN
cord-253024-b393ea2u	53	10	1	1	CD
cord-253024-b393ea2u	53	11	50-cm2	50-cm2	CD
cord-253024-b393ea2u	53	12	flasks	flask	NNS
cord-253024-b393ea2u	53	13	containing	contain	VBG
cord-253024-b393ea2u	53	14	DBT	DBT	NNP
cord-253024-b393ea2u	53	15	cells	cell	NNS
cord-253024-b393ea2u	53	16	as	as	IN
cord-253024-b393ea2u	53	17	described	describe	VBN
cord-253024-b393ea2u	53	18	previously	previously	RB
cord-253024-b393ea2u	53	19	.	.	.
cord-253024-b393ea2u	54	1	Plaque	plaque	NN
cord-253024-b393ea2u	54	2	assays	assay	NNS
cord-253024-b393ea2u	54	3	were	be	VBD
cord-253024-b393ea2u	54	4	performed	perform	VBN
cord-253024-b393ea2u	54	5	at	at	IN
cord-253024-b393ea2u	54	6	either	either	CC
cord-253024-b393ea2u	54	7	the	the	DT
cord-253024-b393ea2u	54	8	permissive	permissive	JJ
cord-253024-b393ea2u	54	9	(	(	-LRB-
cord-253024-b393ea2u	54	10	32	32	CD
cord-253024-b393ea2u	54	11	"	"	''
cord-253024-b393ea2u	54	12	)	)	-RRB-
cord-253024-b393ea2u	54	13	or	or	CC
cord-253024-b393ea2u	54	14	nonpermissive	nonpermissive	NNP
cord-253024-b393ea2u	54	15	(	(	-LRB-
cord-253024-b393ea2u	54	16	39.5	39.5	CD
cord-253024-b393ea2u	54	17	"	"	''
cord-253024-b393ea2u	54	18	)	)	-RRB-
cord-253024-b393ea2u	54	19	temperatures	temperature	NNS
cord-253024-b393ea2u	54	20	in	in	IN
cord-253024-b393ea2u	54	21	DBT	DBT	NNP
cord-253024-b393ea2u	54	22	cells	cell	NNS
cord-253024-b393ea2u	54	23	in	in	IN
cord-253024-b393ea2u	54	24	Dulbecco	Dulbecco	NNP
cord-253024-b393ea2u	54	25	's	's	POS
cord-253024-b393ea2u	54	26	modified	modify	VBN
cord-253024-b393ea2u	54	27	essential	essential	JJ
cord-253024-b393ea2u	54	28	medium	medium	NN
cord-253024-b393ea2u	54	29	(	(	-LRB-
cord-253024-b393ea2u	54	30	DMEM	DMEM	NNP
cord-253024-b393ea2u	54	31	)	)	-RRB-
cord-253024-b393ea2u	55	1	(	(	-LRB-
cord-253024-b393ea2u	55	2	Sigma	Sigma	NNP
cord-253024-b393ea2u	55	3	)	)	-RRB-
cord-253024-b393ea2u	55	4	containing	contain	VBG
cord-253024-b393ea2u	55	5	10	10	CD
cord-253024-b393ea2u	55	6	%	%	NN
cord-253024-b393ea2u	55	7	Nu	Nu	NNP
cord-253024-b393ea2u	55	8	-	-	HYPH
cord-253024-b393ea2u	55	9	serum	serum	NN
cord-253024-b393ea2u	55	10	(	(	-LRB-
cord-253024-b393ea2u	55	11	Collaborative	Collaborative	NNP
cord-253024-b393ea2u	55	12	Research	Research	NNP
cord-253024-b393ea2u	55	13	,	,	,
cord-253024-b393ea2u	55	14	Inc.	Inc.	NNP
cord-253024-b393ea2u	55	15	)	)	-RRB-
cord-253024-b393ea2u	55	16	,	,	,
cord-253024-b393ea2u	55	17	1	1	CD
cord-253024-b393ea2u	55	18	%	%	NN
cord-253024-b393ea2u	55	19	gentamycin	gentamycin	NNS
cord-253024-b393ea2u	55	20	/	/	SYM
cord-253024-b393ea2u	55	21	kanamycin	kanamycin	NNP
cord-253024-b393ea2u	55	22	(	(	-LRB-
cord-253024-b393ea2u	55	23	GIBCO	GIBCO	NNP
cord-253024-b393ea2u	55	24	)	)	-RRB-
cord-253024-b393ea2u	55	25	,	,	,
cord-253024-b393ea2u	55	26	and	and	CC
cord-253024-b393ea2u	55	27	0.8	0.8	CD
cord-253024-b393ea2u	55	28	%	%	NN
cord-253024-b393ea2u	55	29	agarose	agarose	NN
cord-253024-b393ea2u	55	30	(	(	-LRB-
cord-253024-b393ea2u	55	31	BRL	BRL	NNP
cord-253024-b393ea2u	55	32	)	)	-RRB-
cord-253024-b393ea2u	55	33	.	.	.
cord-253024-b393ea2u	56	1	All	all	DT
cord-253024-b393ea2u	56	2	plaque	plaque	NN
cord-253024-b393ea2u	56	3	assays	assay	NNS
cord-253024-b393ea2u	56	4	were	be	VBD
cord-253024-b393ea2u	56	5	stained	stain	VBN
cord-253024-b393ea2u	56	6	28	28	CD
cord-253024-b393ea2u	56	7	-	-	SYM
cord-253024-b393ea2u	56	8	36	36	CD
cord-253024-b393ea2u	56	9	hr	hr	NN
cord-253024-b393ea2u	56	10	postinfection	postinfection	NN
cord-253024-b393ea2u	56	11	by	by	IN
cord-253024-b393ea2u	56	12	the	the	DT
cord-253024-b393ea2u	56	13	addition	addition	NN
cord-253024-b393ea2u	56	14	of	of	IN
cord-253024-b393ea2u	56	15	neutral	neutral	JJ
cord-253024-b393ea2u	56	16	red	red	NN
cord-253024-b393ea2u	56	17	for	for	IN
cord-253024-b393ea2u	56	18	2	2	CD
cord-253024-b393ea2u	56	19	hr	hr	NN
cord-253024-b393ea2u	56	20	.	.	.
cord-253024-b393ea2u	57	1	Cloned	clone	VBN
cord-253024-b393ea2u	57	2	17CI-1	17ci-1	CD
cord-253024-b393ea2u	57	3	cells	cell	NNS
cord-253024-b393ea2u	57	4	were	be	VBD
cord-253024-b393ea2u	57	5	kindly	kindly	RB
cord-253024-b393ea2u	57	6	provided	provide	VBN
cord-253024-b393ea2u	57	7	by	by	IN
cord-253024-b393ea2u	57	8	Dr.	Dr.	NNP
cord-253024-b393ea2u	57	9	Sue	Sue	NNP
cord-253024-b393ea2u	57	10	Baker	Baker	NNP
cord-253024-b393ea2u	57	11	(	(	-LRB-
cord-253024-b393ea2u	57	12	Loyola	Loyola	NNP
cord-253024-b393ea2u	57	13	University	University	NNP
cord-253024-b393ea2u	57	14	,	,	,
cord-253024-b393ea2u	57	15	Chicago	Chicago	NNP
cord-253024-b393ea2u	57	16	)	)	-RRB-
cord-253024-b393ea2u	57	17	and	and	CC
cord-253024-b393ea2u	57	18	maintained	maintain	VBD
cord-253024-b393ea2u	57	19	at	at	IN
cord-253024-b393ea2u	57	20	37	37	CD
cord-253024-b393ea2u	57	21	"	"	''
cord-253024-b393ea2u	57	22	in	in	IN
cord-253024-b393ea2u	57	23	DMEM	DMEM	NNP
cord-253024-b393ea2u	57	24	containing	contain	VBG
cord-253024-b393ea2u	57	25	7	7	CD
cord-253024-b393ea2u	57	26	%	%	NN
cord-253024-b393ea2u	57	27	newborn	newborn	JJ
cord-253024-b393ea2u	57	28	calf	calf	NN
cord-253024-b393ea2u	57	29	serum	serum	NN
cord-253024-b393ea2u	57	30	(	(	-LRB-
cord-253024-b393ea2u	57	31	GIBCO	GIBCO	NNP
cord-253024-b393ea2u	57	32	)	)	-RRB-
cord-253024-b393ea2u	57	33	,	,	,
cord-253024-b393ea2u	57	34	1	1	CD
cord-253024-b393ea2u	57	35	%	%	NN
cord-253024-b393ea2u	57	36	gentamycin	gentamycin	NNS
cord-253024-b393ea2u	57	37	/	/	SYM
cord-253024-b393ea2u	57	38	kanamycin	kanamycin	NNS
cord-253024-b393ea2u	57	39	,	,	,
cord-253024-b393ea2u	57	40	2	2	CD
cord-253024-b393ea2u	57	41	%	%	NN
cord-253024-b393ea2u	57	42	glucose	glucose	NN
cord-253024-b393ea2u	57	43	(	(	-LRB-
cord-253024-b393ea2u	57	44	Sigma	Sigma	NNP
cord-253024-b393ea2u	57	45	)	)	-RRB-
cord-253024-b393ea2u	57	46	,	,	,
cord-253024-b393ea2u	57	47	and	and	CC
cord-253024-b393ea2u	57	48	5	5	CD
cord-253024-b393ea2u	57	49	%	%	NN
cord-253024-b393ea2u	57	50	ttyptose	ttyptose	NN
cord-253024-b393ea2u	57	51	-	-	HYPH
cord-253024-b393ea2u	57	52	phosphate	phosphate	NN
cord-253024-b393ea2u	57	53	broth	broth	NN
cord-253024-b393ea2u	57	54	(	(	-LRB-
cord-253024-b393ea2u	57	55	Sigma	Sigma	NNP
cord-253024-b393ea2u	57	56	)	)	-RRB-
cord-253024-b393ea2u	57	57	.	.	.
cord-253024-b393ea2u	58	1	Most	Most	JJS
cord-253024-b393ea2u	58	2	of	of	IN
cord-253024-b393ea2u	58	3	the	the	DT
cord-253024-b393ea2u	58	4	mutants	mutant	NNS
cord-253024-b393ea2u	58	5	used	use	VBN
cord-253024-b393ea2u	58	6	in	in	IN
cord-253024-b393ea2u	58	7	this	this	DT
cord-253024-b393ea2u	58	8	study	study	NN
cord-253024-b393ea2u	58	9	have	have	VBP
cord-253024-b393ea2u	58	10	been	be	VBN
cord-253024-b393ea2u	58	11	characterized	characterize	VBN
cord-253024-b393ea2u	58	12	previously	previously	RB
cord-253024-b393ea2u	58	13	(	(	-LRB-
cord-253024-b393ea2u	58	14	LA1	LA1	NNP
cord-253024-b393ea2u	58	15	8	8	CD
cord-253024-b393ea2u	58	16	,	,	,
cord-253024-b393ea2u	58	17	LA7	LA7	NNP
cord-253024-b393ea2u	58	18	,	,	,
cord-253024-b393ea2u	58	19	LA1	LA1	NNP
cord-253024-b393ea2u	58	20	2	2	CD
cord-253024-b393ea2u	58	21	,	,	,
cord-253024-b393ea2u	58	22	NC6	NC6	NNP
cord-253024-b393ea2u	58	23	)	)	-RRB-
cord-253024-b393ea2u	58	24	.	.	.
cord-253024-b393ea2u	59	1	To	to	TO
cord-253024-b393ea2u	59	2	increase	increase	VB
cord-253024-b393ea2u	59	3	the	the	DT
cord-253024-b393ea2u	59	4	accuracy	accuracy	NN
cord-253024-b393ea2u	59	5	of	of	IN
cord-253024-b393ea2u	59	6	our	-PRON-	PRP$
cord-253024-b393ea2u	59	7	recombination	recombination	NN
cord-253024-b393ea2u	59	8	mapping	mapping	NN
cord-253024-b393ea2u	59	9	data	datum	NNS
cord-253024-b393ea2u	59	10	,	,	,
cord-253024-b393ea2u	59	11	additional	additional	JJ
cord-253024-b393ea2u	59	12	mutants	mutant	NNS
cord-253024-b393ea2u	59	13	residing	reside	VBG
cord-253024-b393ea2u	59	14	within	within	IN
cord-253024-b393ea2u	59	15	complementation	complementation	NN
cord-253024-b393ea2u	59	16	group	group	NN
cord-253024-b393ea2u	59	17	F	F	NNP
cord-253024-b393ea2u	59	18	were	be	VBD
cord-253024-b393ea2u	59	19	isolated	isolate	VBN
cord-253024-b393ea2u	59	20	.	.	.
cord-253024-b393ea2u	60	1	Briefly	briefly	RB
cord-253024-b393ea2u	60	2	,	,	,
cord-253024-b393ea2u	60	3	cultures	culture	NNS
cord-253024-b393ea2u	60	4	of	of	IN
cord-253024-b393ea2u	60	5	MHV	MHV	NNP
cord-253024-b393ea2u	60	6	-	-	HYPH
cord-253024-b393ea2u	60	7	A59-infected	a59-infecte	VBN
cord-253024-b393ea2u	60	8	cells	cell	NNS
cord-253024-b393ea2u	60	9	were	be	VBD
cord-253024-b393ea2u	60	10	mutagenized	mutagenize	VBN
cord-253024-b393ea2u	60	11	with	with	IN
cord-253024-b393ea2u	60	12	either	either	CC
cord-253024-b393ea2u	60	13	20	20	CD
cord-253024-b393ea2u	60	14	pug	pug	NN
cord-253024-b393ea2u	60	15	/	/	SYM
cord-253024-b393ea2u	60	16	ml	ml	NN
cord-253024-b393ea2u	60	17	5-azacytidine	5-azacytidine	CD
cord-253024-b393ea2u	60	18	or	or	CC
cord-253024-b393ea2u	61	1	100	100	CD
cord-253024-b393ea2u	61	2	pg/	pg/	NNS
cord-253024-b393ea2u	61	3	ml	ml	NNP
cord-253024-b393ea2u	61	4	5-fluorouracil	5-fluorouracil	CD
cord-253024-b393ea2u	61	5	for	for	IN
cord-253024-b393ea2u	61	6	12	12	CD
cord-253024-b393ea2u	61	7	hr	hr	NN
cord-253024-b393ea2u	61	8	.	.	.
cord-253024-b393ea2u	62	1	Supernatant	supernatant	JJ
cord-253024-b393ea2u	62	2	fluids	fluid	NNS
cord-253024-b393ea2u	62	3	containing	contain	VBG
cord-253024-b393ea2u	62	4	mutagenized	mutagenized	JJ
cord-253024-b393ea2u	62	5	virus	virus	NN
cord-253024-b393ea2u	62	6	were	be	VBD
cord-253024-b393ea2u	62	7	plaqued	plaque	VBN
cord-253024-b393ea2u	62	8	at	at	IN
cord-253024-b393ea2u	62	9	permissive	permissive	JJ
cord-253024-b393ea2u	62	10	temperature	temperature	NN
cord-253024-b393ea2u	62	11	and	and	CC
cord-253024-b393ea2u	62	12	individual	individual	JJ
cord-253024-b393ea2u	62	13	plaques	plaque	NNS
cord-253024-b393ea2u	62	14	were	be	VBD
cord-253024-b393ea2u	62	15	isolated	isolate	VBN
cord-253024-b393ea2u	62	16	.	.	.
cord-253024-b393ea2u	63	1	Each	each	DT
cord-253024-b393ea2u	63	2	plaque	plaque	NN
cord-253024-b393ea2u	63	3	was	be	VBD
cord-253024-b393ea2u	63	4	diluted	dilute	VBN
cord-253024-b393ea2u	63	5	into	into	IN
cord-253024-b393ea2u	63	6	0.5	0.5	CD
cord-253024-b393ea2u	63	7	ml	ml	NN
cord-253024-b393ea2u	63	8	ice	ice	NN
cord-253024-b393ea2u	63	9	-	-	HYPH
cord-253024-b393ea2u	63	10	cold	cold	JJ
cord-253024-b393ea2u	63	11	PBS	PBS	NNP
cord-253024-b393ea2u	63	12	,	,	,
cord-253024-b393ea2u	63	13	screened	screen	VBN
cord-253024-b393ea2u	63	14	at	at	IN
cord-253024-b393ea2u	63	15	32	32	CD
cord-253024-b393ea2u	63	16	"	"	''
cord-253024-b393ea2u	63	17	and	and	CC
cord-253024-b393ea2u	63	18	39.5	39.5	CD
cord-253024-b393ea2u	63	19	"	"	''
cord-253024-b393ea2u	63	20	by	by	IN
cord-253024-b393ea2u	63	21	plaque	plaque	NN
cord-253024-b393ea2u	63	22	assay	assay	NN
cord-253024-b393ea2u	63	23	,	,	,
cord-253024-b393ea2u	63	24	and	and	CC
cord-253024-b393ea2u	63	25	isolates	isolate	NNS
cord-253024-b393ea2u	63	26	displaying	display	VBG
cord-253024-b393ea2u	63	27	ts	ts	JJ
cord-253024-b393ea2u	63	28	phenotypes	phenotype	NNS
cord-253024-b393ea2u	63	29	were	be	VBD
cord-253024-b393ea2u	63	30	repurified	repurifie	VBN
cord-253024-b393ea2u	63	31	two	two	CD
cord-253024-b393ea2u	63	32	to	to	TO
cord-253024-b393ea2u	63	33	three	three	CD
cord-253024-b393ea2u	63	34	times	time	NNS
cord-253024-b393ea2u	63	35	at	at	IN
cord-253024-b393ea2u	63	36	32	32	CD
cord-253024-b393ea2u	63	37	"	"	''
cord-253024-b393ea2u	63	38	.	.	.
cord-253024-b393ea2u	64	1	Virus	virus	NN
cord-253024-b393ea2u	64	2	stocks	stock	NNS
cord-253024-b393ea2u	64	3	were	be	VBD
cord-253024-b393ea2u	64	4	assayed	assay	VBN
cord-253024-b393ea2u	64	5	at	at	IN
cord-253024-b393ea2u	64	6	the	the	DT
cord-253024-b393ea2u	64	7	permissive	permissive	JJ
cord-253024-b393ea2u	64	8	and	and	CC
cord-253024-b393ea2u	64	9	restrictive	restrictive	JJ
cord-253024-b393ea2u	64	10	temperatures	temperature	NNS
cord-253024-b393ea2u	64	11	and	and	CC
cord-253024-b393ea2u	64	12	only	only	RB
cord-253024-b393ea2u	64	13	those	those	DT
cord-253024-b393ea2u	64	14	isolates	isolate	NNS
cord-253024-b393ea2u	64	15	displaying	display	VBG
cord-253024-b393ea2u	64	16	reversion	reversion	NN
cord-253024-b393ea2u	64	17	frequencies	frequency	NNS
cord-253024-b393ea2u	64	18	of	of	IN
cord-253024-b393ea2u	64	19	less	less	JJR
cord-253024-b393ea2u	64	20	than	than	IN
cord-253024-b393ea2u	64	21	1	1	CD
cord-253024-b393ea2u	64	22	OM3	om3	NN
cord-253024-b393ea2u	64	23	were	be	VBD
cord-253024-b393ea2u	64	24	used	use	VBN
cord-253024-b393ea2u	64	25	for	for	IN
cord-253024-b393ea2u	64	26	future	future	JJ
cord-253024-b393ea2u	64	27	studies	study	NNS
cord-253024-b393ea2u	64	28	.	.	.
cord-253024-b393ea2u	65	1	Complementation	Complementation	NNP
cord-253024-b393ea2u	65	2	and	and	CC
cord-253024-b393ea2u	65	3	RNA	RNA	NNP
cord-253024-b393ea2u	65	4	phenotype	phenotype	NN
cord-253024-b393ea2u	65	5	analysis	analysis	NN
cord-253024-b393ea2u	65	6	were	be	VBD
cord-253024-b393ea2u	65	7	performed	perform	VBN
cord-253024-b393ea2u	65	8	as	as	IN
cord-253024-b393ea2u	65	9	described	describe	VBN
cord-253024-b393ea2u	65	10	previously	previously	RB
cord-253024-b393ea2u	66	1	(	(	-LRB-
cord-253024-b393ea2u	66	2	Leibowitz	Leibowitz	NNP
cord-253024-b393ea2u	66	3	et	et	NNP
cord-253024-b393ea2u	66	4	al	al	NNP
cord-253024-b393ea2u	66	5	.	.	NNP
cord-253024-b393ea2u	66	6	,	,	,
cord-253024-b393ea2u	66	7	1982	1982	CD
cord-253024-b393ea2u	66	8	;	;	:
cord-253024-b393ea2u	66	9	Martin	Martin	NNP
cord-253024-b393ea2u	66	10	et	et	NNP
cord-253024-b393ea2u	66	11	al	al	NNP
cord-253024-b393ea2u	66	12	.	.	NNP
cord-253024-b393ea2u	66	13	,	,	,
cord-253024-b393ea2u	66	14	1988	1988	CD
cord-253024-b393ea2u	66	15	;	;	:
cord-253024-b393ea2u	66	16	,	,	,
cord-253024-b393ea2u	66	17	and	and	CC
cord-253024-b393ea2u	66	18	three	three	CD
cord-253024-b393ea2u	66	19	additional	additional	JJ
cord-253024-b393ea2u	66	20	group	group	NN
cord-253024-b393ea2u	66	21	F	F	NNP
cord-253024-b393ea2u	66	22	RNA+	RNA+	NNP
cord-253024-b393ea2u	66	23	mutants	mutant	NNS
cord-253024-b393ea2u	66	24	were	be	VBD
cord-253024-b393ea2u	66	25	isolated	isolate	VBN
cord-253024-b393ea2u	66	26	and	and	CC
cord-253024-b393ea2u	66	27	designated	designate	VBN
cord-253024-b393ea2u	66	28	NC14	NC14	NNP
cord-253024-b393ea2u	66	29	,	,	,
cord-253024-b393ea2u	66	30	NC1	NC1	NNP
cord-253024-b393ea2u	66	31	6	6	CD
cord-253024-b393ea2u	66	32	,	,	,
cord-253024-b393ea2u	66	33	and	and	CC
cord-253024-b393ea2u	66	34	NC1	NC1	NNP
cord-253024-b393ea2u	66	35	7	7	CD
cord-253024-b393ea2u	66	36	.	.	.
cord-253024-b393ea2u	67	1	NC1	NC1	NNP
cord-253024-b393ea2u	67	2	4	4	CD
cord-253024-b393ea2u	67	3	and	and	CC
cord-253024-b393ea2u	67	4	NC1	NC1	NNP
cord-253024-b393ea2u	67	5	6	6	CD
cord-253024-b393ea2u	67	6	were	be	VBD
cord-253024-b393ea2u	67	7	isolated	isolate	VBN
cord-253024-b393ea2u	67	8	using	use	VBG
cord-253024-b393ea2u	67	9	5-fluorouracil	5-fluorouracil	CD
cord-253024-b393ea2u	67	10	,	,	,
cord-253024-b393ea2u	67	11	while	while	IN
cord-253024-b393ea2u	67	12	NC	NC	NNP
cord-253024-b393ea2u	67	13	17	17	CD
cord-253024-b393ea2u	67	14	was	be	VBD
cord-253024-b393ea2u	67	15	isolated	isolate	VBN
cord-253024-b393ea2u	67	16	using	use	VBG
cord-253024-b393ea2u	67	17	5-azacytidine	5-azacytidine	CD
cord-253024-b393ea2u	67	18	.	.	.
cord-253024-b393ea2u	68	1	To	to	TO
cord-253024-b393ea2u	68	2	select	select	VB
cord-253024-b393ea2u	68	3	for	for	IN
cord-253024-b393ea2u	68	4	revertants	revertant	NNS
cord-253024-b393ea2u	68	5	from	from	IN
cord-253024-b393ea2u	68	6	the	the	DT
cord-253024-b393ea2u	68	7	group	group	NN
cord-253024-b393ea2u	68	8	F	F	NNP
cord-253024-b393ea2u	68	9	RNA+	RNA+	NNP
cord-253024-b393ea2u	68	10	ts	ts	NN
cord-253024-b393ea2u	68	11	mutants	mutant	NNS
cord-253024-b393ea2u	68	12	,	,	,
cord-253024-b393ea2u	68	13	virus	virus	NN
cord-253024-b393ea2u	68	14	stocks	stock	NNS
cord-253024-b393ea2u	68	15	were	be	VBD
cord-253024-b393ea2u	68	16	plaque	plaque	NN
cord-253024-b393ea2u	68	17	assayed	assay	VBN
cord-253024-b393ea2u	68	18	at	at	IN
cord-253024-b393ea2u	68	19	39.5	39.5	CD
cord-253024-b393ea2u	68	20	"	"	''
cord-253024-b393ea2u	68	21	,	,	,
cord-253024-b393ea2u	68	22	and	and	CC
cord-253024-b393ea2u	68	23	individual	individual	JJ
cord-253024-b393ea2u	68	24	plaques	plaque	NNS
cord-253024-b393ea2u	68	25	were	be	VBD
cord-253024-b393ea2u	68	26	isolated	isolate	VBN
cord-253024-b393ea2u	68	27	and	and	CC
cord-253024-b393ea2u	68	28	repurified	repurifie	VBN
cord-253024-b393ea2u	68	29	by	by	IN
cord-253024-b393ea2u	68	30	plague	plague	NN
cord-253024-b393ea2u	68	31	assay	assay	NN
cord-253024-b393ea2u	68	32	at	at	IN
cord-253024-b393ea2u	68	33	the	the	DT
cord-253024-b393ea2u	68	34	restrictive	restrictive	JJ
cord-253024-b393ea2u	68	35	temperature	temperature	NN
cord-253024-b393ea2u	68	36	.	.	.
cord-253024-b393ea2u	69	1	Individual	individual	JJ
cord-253024-b393ea2u	69	2	stocks	stock	NNS
cord-253024-b393ea2u	69	3	of	of	IN
cord-253024-b393ea2u	69	4	revertant	revertant	NN
cord-253024-b393ea2u	69	5	virus	virus	NN
cord-253024-b393ea2u	69	6	were	be	VBD
cord-253024-b393ea2u	69	7	grown	grow	VBN
cord-253024-b393ea2u	69	8	in	in	IN
cord-253024-b393ea2u	69	9	1	1	CD
cord-253024-b393ea2u	69	10	50-cm2	50-cm2	CD
cord-253024-b393ea2u	69	11	flasks	flask	NNS
cord-253024-b393ea2u	69	12	at	at	IN
cord-253024-b393ea2u	69	13	37	37	CD
cord-253024-b393ea2u	69	14	"	"	''
cord-253024-b393ea2u	69	15	and	and	CC
cord-253024-b393ea2u	69	16	the	the	DT
cord-253024-b393ea2u	69	17	reversion	reversion	NN
cord-253024-b393ea2u	69	18	frequency	frequency	NN
cord-253024-b393ea2u	69	19	of	of	IN
cord-253024-b393ea2u	69	20	each	each	DT
cord-253024-b393ea2u	69	21	was	be	VBD
cord-253024-b393ea2u	69	22	determined	determine	VBN
cord-253024-b393ea2u	69	23	by	by	IN
cord-253024-b393ea2u	69	24	plague	plague	NN
cord-253024-b393ea2u	69	25	assay	assay	NN
cord-253024-b393ea2u	69	26	at	at	IN
cord-253024-b393ea2u	69	27	32	32	CD
cord-253024-b393ea2u	69	28	and	and	CC
cord-253024-b393ea2u	69	29	39.5	39.5	CD
cord-253024-b393ea2u	69	30	"	"	''
cord-253024-b393ea2u	69	31	.	.	.
cord-253024-b393ea2u	70	1	At	at	RB
cord-253024-b393ea2u	70	2	least	least	RBS
cord-253024-b393ea2u	70	3	three	three	CD
cord-253024-b393ea2u	70	4	independent	independent	JJ
cord-253024-b393ea2u	70	5	revertants	revertant	NNS
cord-253024-b393ea2u	70	6	from	from	IN
cord-253024-b393ea2u	70	7	each	each	DT
cord-253024-b393ea2u	70	8	RNA+	RNA+	NNP
cord-253024-b393ea2u	70	9	mutant	mutant	NN
cord-253024-b393ea2u	70	10	were	be	VBD
cord-253024-b393ea2u	70	11	isolated	isolate	VBN
cord-253024-b393ea2u	70	12	.	.	.
cord-253024-b393ea2u	71	1	To	to	TO
cord-253024-b393ea2u	71	2	isolate	isolate	VB
cord-253024-b393ea2u	71	3	RNA	RNA	NNP
cord-253024-b393ea2u	71	4	recombinant	recombinant	JJ
cord-253024-b393ea2u	71	5	virus	virus	NN
cord-253024-b393ea2u	71	6	,	,	,
cord-253024-b393ea2u	71	7	DBT	DBT	NNP
cord-253024-b393ea2u	71	8	cells	cell	NNS
cord-253024-b393ea2u	71	9	were	be	VBD
cord-253024-b393ea2u	71	10	coinfected	coinfecte	VBN
cord-253024-b393ea2u	71	11	with	with	IN
cord-253024-b393ea2u	71	12	two	two	CD
cord-253024-b393ea2u	71	13	different	different	JJ
cord-253024-b393ea2u	71	14	ts	ts	NN
cord-253024-b393ea2u	71	15	mutants	mutant	NNS
cord-253024-b393ea2u	71	16	at	at	IN
cord-253024-b393ea2u	71	17	an	an	DT
cord-253024-b393ea2u	71	18	m.o.i	m.o.i	NN
cord-253024-b393ea2u	71	19	.	.	NN
cord-253024-b393ea2u	71	20	of	of	IN
cord-253024-b393ea2u	71	21	10	10	CD
cord-253024-b393ea2u	71	22	each	each	DT
cord-253024-b393ea2u	71	23	at	at	IN
cord-253024-b393ea2u	71	24	32	32	CD
cord-253024-b393ea2u	71	25	"	"	''
cord-253024-b393ea2u	71	26	.	.	.
cord-253024-b393ea2u	72	1	The	the	DT
cord-253024-b393ea2u	72	2	medium	medium	NN
cord-253024-b393ea2u	72	3	was	be	VBD
cord-253024-b393ea2u	72	4	harvested	harvest	VBN
cord-253024-b393ea2u	72	5	at	at	IN
cord-253024-b393ea2u	72	6	16	16	CD
cord-253024-b393ea2u	72	7	hr	hr	NN
cord-253024-b393ea2u	72	8	postinfection	postinfection	NN
cord-253024-b393ea2u	72	9	and	and	CC
cord-253024-b393ea2u	72	10	stored	store	VBN
cord-253024-b393ea2u	72	11	at	at	IN
cord-253024-b393ea2u	72	12	-70	-70	NN
cord-253024-b393ea2u	72	13	"	"	''
cord-253024-b393ea2u	72	14	.	.	.
cord-253024-b393ea2u	73	1	Recombinant	recombinant	JJ
cord-253024-b393ea2u	73	2	viruses	virus	NNS
cord-253024-b393ea2u	73	3	were	be	VBD
cord-253024-b393ea2u	73	4	isolated	isolate	VBN
cord-253024-b393ea2u	73	5	at	at	IN
cord-253024-b393ea2u	73	6	the	the	DT
cord-253024-b393ea2u	73	7	restrictive	restrictive	JJ
cord-253024-b393ea2u	73	8	temperature	temperature	NN
cord-253024-b393ea2u	73	9	,	,	,
cord-253024-b393ea2u	73	10	repurified	repurifie	VBN
cord-253024-b393ea2u	73	11	at	at	IN
cord-253024-b393ea2u	73	12	39.5	39.5	CD
cord-253024-b393ea2u	73	13	"	"	''
cord-253024-b393ea2u	73	14	,	,	,
cord-253024-b393ea2u	73	15	and	and	CC
cord-253024-b393ea2u	73	16	grown	grow	VBN
cord-253024-b393ea2u	73	17	in	in	IN
cord-253024-b393ea2u	73	18	DBT	DBT	NNP
cord-253024-b393ea2u	73	19	cells	cell	NNS
cord-253024-b393ea2u	73	20	at	at	IN
cord-253024-b393ea2u	73	21	37	37	CD
cord-253024-b393ea2u	73	22	"	"	''
cord-253024-b393ea2u	73	23	.	.	.
cord-253024-b393ea2u	74	1	Culture	culture	NN
cord-253024-b393ea2u	74	2	of	of	IN
cord-253024-b393ea2u	74	3	17CL-1	17cl-1	CD
cord-253024-b393ea2u	74	4	cells	cell	NNS
cord-253024-b393ea2u	74	5	(	(	-LRB-
cord-253024-b393ea2u	74	6	1	1	CD
cord-253024-b393ea2u	74	7	X	x	NN
cord-253024-b393ea2u	74	8	106	106	CD
cord-253024-b393ea2u	74	9	)	)	-RRB-
cord-253024-b393ea2u	74	10	in	in	IN
cord-253024-b393ea2u	74	11	25-mm	25-mm	CD
cord-253024-b393ea2u	74	12	six	six	CD
cord-253024-b393ea2u	74	13	-	-	HYPH
cord-253024-b393ea2u	74	14	well	well	RB
cord-253024-b393ea2u	74	15	dishes	dish	NNS
cord-253024-b393ea2u	74	16	(	(	-LRB-
cord-253024-b393ea2u	74	17	Costar	Costar	NNP
cord-253024-b393ea2u	74	18	)	)	-RRB-
cord-253024-b393ea2u	74	19	were	be	VBD
cord-253024-b393ea2u	74	20	infected	infect	VBN
cord-253024-b393ea2u	74	21	with	with	IN
cord-253024-b393ea2u	74	22	various	various	JJ
cord-253024-b393ea2u	74	23	group	group	NN
cord-253024-b393ea2u	74	24	F	f	NN
cord-253024-b393ea2u	74	25	ts	ts	NNP
cord-253024-b393ea2u	74	26	mutants	mutant	NNS
cord-253024-b393ea2u	74	27	,	,	,
cord-253024-b393ea2u	74	28	revertants	revertant	NNS
cord-253024-b393ea2u	74	29	,	,	,
cord-253024-b393ea2u	74	30	or	or	CC
cord-253024-b393ea2u	74	31	recombinant	recombinant	JJ
cord-253024-b393ea2u	74	32	viruses	virus	NNS
cord-253024-b393ea2u	74	33	at	at	IN
cord-253024-b393ea2u	74	34	a	a	DT
cord-253024-b393ea2u	74	35	multiplicity	multiplicity	NN
cord-253024-b393ea2u	74	36	of	of	IN
cord-253024-b393ea2u	74	37	Infection	infection	NN
cord-253024-b393ea2u	74	38	of	of	IN
cord-253024-b393ea2u	74	39	10	10	CD
cord-253024-b393ea2u	74	40	for	for	IN
cord-253024-b393ea2u	74	41	1	1	CD
cord-253024-b393ea2u	74	42	hr	hr	NN
cord-253024-b393ea2u	74	43	at	at	IN
cord-253024-b393ea2u	74	44	room	room	NN
cord-253024-b393ea2u	74	45	temperature	temperature	NN
cord-253024-b393ea2u	74	46	.	.	.
cord-253024-b393ea2u	75	1	Following	follow	VBG
cord-253024-b393ea2u	75	2	adsorption	adsorption	NN
cord-253024-b393ea2u	75	3	,	,	,
cord-253024-b393ea2u	75	4	the	the	DT
cord-253024-b393ea2u	75	5	inoculum	inoculum	NN
cord-253024-b393ea2u	75	6	was	be	VBD
cord-253024-b393ea2u	75	7	removed	remove	VBN
cord-253024-b393ea2u	75	8	,	,	,
cord-253024-b393ea2u	75	9	and	and	CC
cord-253024-b393ea2u	75	10	the	the	DT
cord-253024-b393ea2u	75	11	cultures	culture	NNS
cord-253024-b393ea2u	75	12	were	be	VBD
cord-253024-b393ea2u	75	13	rinsed	rinse	VBN
cord-253024-b393ea2u	75	14	with	with	IN
cord-253024-b393ea2u	75	15	PBS	PBS	NNP
cord-253024-b393ea2u	75	16	and	and	CC
cord-253024-b393ea2u	75	17	incubated	incubate	VBN
cord-253024-b393ea2u	75	18	in	in	IN
cord-253024-b393ea2u	75	19	complete	complete	JJ
cord-253024-b393ea2u	75	20	DMEM	DMEM	NNP
cord-253024-b393ea2u	75	21	for	for	IN
cord-253024-b393ea2u	75	22	4	4	CD
cord-253024-b393ea2u	75	23	hr	hr	NN
cord-253024-b393ea2u	75	24	at	at	IN
cord-253024-b393ea2u	75	25	32	32	CD
cord-253024-b393ea2u	75	26	"	"	''
cord-253024-b393ea2u	75	27	.	.	.
cord-253024-b393ea2u	76	1	Actinomycin	Actinomycin	NNP
cord-253024-b393ea2u	76	2	D	D	NNP
cord-253024-b393ea2u	76	3	(	(	-LRB-
cord-253024-b393ea2u	76	4	2	2	CD
cord-253024-b393ea2u	76	5	PgI	PgI	NNP
cord-253024-b393ea2u	76	6	ml	ml	NNP
cord-253024-b393ea2u	76	7	)	)	-RRB-
cord-253024-b393ea2u	76	8	was	be	VBD
cord-253024-b393ea2u	76	9	added	add	VBN
cord-253024-b393ea2u	76	10	for	for	IN
cord-253024-b393ea2u	76	11	1	1	CD
cord-253024-b393ea2u	76	12	hr	hr	NN
cord-253024-b393ea2u	76	13	and	and	CC
cord-253024-b393ea2u	76	14	one	one	CD
cord-253024-b393ea2u	76	15	-	-	HYPH
cord-253024-b393ea2u	76	16	half	half	NN
cord-253024-b393ea2u	76	17	the	the	DT
cord-253024-b393ea2u	76	18	cultures	culture	NNS
cord-253024-b393ea2u	76	19	shifted	shift	VBD
cord-253024-b393ea2u	76	20	to	to	IN
cord-253024-b393ea2u	76	21	the	the	DT
cord-253024-b393ea2u	76	22	restrictive	restrictive	JJ
cord-253024-b393ea2u	76	23	temperature	temperature	NN
cord-253024-b393ea2u	76	24	at	at	IN
cord-253024-b393ea2u	76	25	5	5	CD
cord-253024-b393ea2u	76	26	hr	hr	NN
cord-253024-b393ea2u	76	27	postinfection	postinfection	NN
cord-253024-b393ea2u	76	28	by	by	IN
cord-253024-b393ea2u	76	29	the	the	DT
cord-253024-b393ea2u	76	30	addition	addition	NN
cord-253024-b393ea2u	76	31	of	of	IN
cord-253024-b393ea2u	76	32	prewarmed	prewarmed	JJ
cord-253024-b393ea2u	76	33	media	medium	NNS
cord-253024-b393ea2u	76	34	.	.	.
cord-253024-b393ea2u	77	1	Viral	viral	JJ
cord-253024-b393ea2u	77	2	progeny	progeny	NNS
cord-253024-b393ea2u	77	3	were	be	VBD
cord-253024-b393ea2u	77	4	harvested	harvest	VBN
cord-253024-b393ea2u	77	5	at	at	IN
cord-253024-b393ea2u	77	6	varying	vary	VBG
cord-253024-b393ea2u	77	7	intervals	interval	NNS
cord-253024-b393ea2u	77	8	and	and	CC
cord-253024-b393ea2u	77	9	analyzed	analyze	VBN
cord-253024-b393ea2u	77	10	by	by	IN
cord-253024-b393ea2u	77	11	plaque	plaque	NN
cord-253024-b393ea2u	77	12	assay	assay	NN
cord-253024-b393ea2u	77	13	.	.	.
cord-253024-b393ea2u	78	1	To	to	TO
cord-253024-b393ea2u	78	2	determine	determine	VB
cord-253024-b393ea2u	78	3	whether	whether	IN
cord-253024-b393ea2u	78	4	these	these	DT
cord-253024-b393ea2u	78	5	mutants	mutant	NNS
cord-253024-b393ea2u	78	6	could	could	MD
cord-253024-b393ea2u	78	7	transcribe	transcribe	VB
cord-253024-b393ea2u	78	8	viral	viral	JJ
cord-253024-b393ea2u	78	9	mRNA	mrna	NN
cord-253024-b393ea2u	78	10	,	,	,
cord-253024-b393ea2u	78	11	cultures	culture	NNS
cord-253024-b393ea2u	78	12	of	of	IN
cord-253024-b393ea2u	78	13	cells	cell	NNS
cord-253024-b393ea2u	78	14	were	be	VBD
cord-253024-b393ea2u	78	15	infected	infect	VBN
cord-253024-b393ea2u	78	16	with	with	IN
cord-253024-b393ea2u	78	17	various	various	JJ
cord-253024-b393ea2u	78	18	ts	ts	NN
cord-253024-b393ea2u	78	19	mutants	mutant	NNS
cord-253024-b393ea2u	78	20	and	and	CC
cord-253024-b393ea2u	78	21	maintained	maintain	VBD
cord-253024-b393ea2u	78	22	at	at	IN
cord-253024-b393ea2u	78	23	the	the	DT
cord-253024-b393ea2u	78	24	permissive	permissive	JJ
cord-253024-b393ea2u	78	25	or	or	CC
cord-253024-b393ea2u	78	26	restrictive	restrictive	JJ
cord-253024-b393ea2u	78	27	temperature	temperature	NN
cord-253024-b393ea2u	78	28	for	for	IN
cord-253024-b393ea2u	78	29	8	8	CD
cord-253024-b393ea2u	78	30	hr	hr	NN
cord-253024-b393ea2u	78	31	.	.	.
cord-253024-b393ea2u	79	1	The	the	DT
cord-253024-b393ea2u	79	2	media	medium	NNS
cord-253024-b393ea2u	79	3	were	be	VBD
cord-253024-b393ea2u	79	4	removed	remove	VBN
cord-253024-b393ea2u	79	5	and	and	CC
cord-253024-b393ea2u	79	6	the	the	DT
cord-253024-b393ea2u	79	7	cultures	culture	NNS
cord-253024-b393ea2u	79	8	washed	wash	VBD
cord-253024-b393ea2u	79	9	with	with	IN
cord-253024-b393ea2u	79	10	2	2	CD
cord-253024-b393ea2u	79	11	ml	ml	NNS
cord-253024-b393ea2u	79	12	of	of	IN
cord-253024-b393ea2u	79	13	ISO	ISO	NNP
cord-253024-b393ea2u	79	14	-	-	HYPH
cord-253024-b393ea2u	79	15	TKM	TKM	NNP
cord-253024-b393ea2u	79	16	(	(	-LRB-
cord-253024-b393ea2u	79	17	10	10	CD
cord-253024-b393ea2u	79	18	m	m	NN
cord-253024-b393ea2u	79	19	/	/	SYM
cord-253024-b393ea2u	79	20	W	W	NNP
cord-253024-b393ea2u	79	21	Tris	Tris	NNP
cord-253024-b393ea2u	79	22	-	-	HYPH
cord-253024-b393ea2u	79	23	HCI	HCI	NNP
cord-253024-b393ea2u	79	24	,	,	,
cord-253024-b393ea2u	79	25	pH	pH	NNP
cord-253024-b393ea2u	79	26	7.5	7.5	CD
cord-253024-b393ea2u	79	27	,	,	,
cord-253024-b393ea2u	79	28	150	150	CD
cord-253024-b393ea2u	79	29	mNI	mNI	NNP
cord-253024-b393ea2u	79	30	KCI	KCI	NNP
cord-253024-b393ea2u	79	31	,	,	,
cord-253024-b393ea2u	79	32	and	and	CC
cord-253024-b393ea2u	79	33	1.5	1.5	CD
cord-253024-b393ea2u	79	34	mM	mm	CD
cord-253024-b393ea2u	79	35	MgCI	MgCI	NNP
cord-253024-b393ea2u	79	36	,	,	,
cord-253024-b393ea2u	79	37	)	)	-RRB-
cord-253024-b393ea2u	79	38	.	.	.
cord-253024-b393ea2u	80	1	The	the	DT
cord-253024-b393ea2u	80	2	RNA	RNA	NNP
cord-253024-b393ea2u	80	3	was	be	VBD
cord-253024-b393ea2u	80	4	isolated	isolate	VBN
cord-253024-b393ea2u	80	5	as	as	IN
cord-253024-b393ea2u	80	6	described	describe	VBN
cord-253024-b393ea2u	80	7	by	by	IN
cord-253024-b393ea2u	80	8	Sawicki	Sawicki	NNP
cord-253024-b393ea2u	80	9	and	and	CC
cord-253024-b393ea2u	80	10	Sawicki	Sawicki	NNP
cord-253024-b393ea2u	80	11	,	,	,
cord-253024-b393ea2u	80	12	1990	1990	CD
cord-253024-b393ea2u	80	13	,	,	,
cord-253024-b393ea2u	80	14	and	and	CC
cord-253024-b393ea2u	80	15	extensively	extensively	RB
cord-253024-b393ea2u	80	16	purified	purify	VBN
cord-253024-b393ea2u	80	17	by	by	IN
cord-253024-b393ea2u	80	18	successive	successive	JJ
cord-253024-b393ea2u	80	19	rounds	round	NNS
cord-253024-b393ea2u	80	20	of	of	IN
cord-253024-b393ea2u	80	21	phenol	phenol	NN
cord-253024-b393ea2u	80	22	(	(	-LRB-
cord-253024-b393ea2u	80	23	U.S.	U.S.	NNP
cord-253024-b393ea2u	80	24	Biochemical	Biochemical	NNP
cord-253024-b393ea2u	80	25	Company	Company	NNP
cord-253024-b393ea2u	80	26	)	)	-RRB-
cord-253024-b393ea2u	80	27	,	,	,
cord-253024-b393ea2u	80	28	phenol	phenol	NN
cord-253024-b393ea2u	80	29	/	/	SYM
cord-253024-b393ea2u	80	30	chloroform	chloroform	NN
cord-253024-b393ea2u	80	31	/	/	SYM
cord-253024-b393ea2u	80	32	isoamy	isoamy	NN
cord-253024-b393ea2u	80	33	alcohol	alcohol	NN
cord-253024-b393ea2u	80	34	,	,	,
cord-253024-b393ea2u	80	35	and	and	CC
cord-253024-b393ea2u	80	36	chloroform	chloroform	NN
cord-253024-b393ea2u	80	37	extraction	extraction	NN
cord-253024-b393ea2u	80	38	.	.	.
cord-253024-b393ea2u	81	1	The	the	DT
cord-253024-b393ea2u	81	2	RNA	RNA	NNP
cord-253024-b393ea2u	81	3	was	be	VBD
cord-253024-b393ea2u	81	4	bound	bind	VBN
cord-253024-b393ea2u	81	5	to	to	IN
cord-253024-b393ea2u	81	6	nitrocellulose	nitrocellulose	NN
cord-253024-b393ea2u	81	7	filters	filter	NNS
cord-253024-b393ea2u	81	8	and	and	CC
cord-253024-b393ea2u	81	9	probed	probe	VBN
cord-253024-b393ea2u	81	10	with	with	IN
cord-253024-b393ea2u	81	11	radiolabeled	radiolabele	VBN
cord-253024-b393ea2u	81	12	RNA	RNA	NNP
cord-253024-b393ea2u	81	13	probes	probe	NNS
cord-253024-b393ea2u	81	14	specific	specific	JJ
cord-253024-b393ea2u	81	15	for	for	IN
cord-253024-b393ea2u	81	16	the	the	DT
cord-253024-b393ea2u	81	17	MHV	MHV	NNP
cord-253024-b393ea2u	81	18	N	N	NNP
cord-253024-b393ea2u	81	19	gene	gene	NN
cord-253024-b393ea2u	81	20	.	.	.
cord-253024-b393ea2u	82	1	The	the	DT
cord-253024-b393ea2u	82	2	blots	blot	NNS
cord-253024-b393ea2u	82	3	were	be	VBD
cord-253024-b393ea2u	82	4	washed	wash	VBN
cord-253024-b393ea2u	82	5	and	and	CC
cord-253024-b393ea2u	82	6	exposed	expose	VBN
cord-253024-b393ea2u	82	7	to	to	IN
cord-253024-b393ea2u	82	8	XAR-5	XAR-5	NNP
cord-253024-b393ea2u	82	9	film	film	NN
cord-253024-b393ea2u	82	10	for	for	IN
cord-253024-b393ea2u	82	11	24	24	CD
cord-253024-b393ea2u	82	12	hr	hr	NN
cord-253024-b393ea2u	82	13	.	.	.
cord-253024-b393ea2u	83	1	Recombination	recombination	NN
cord-253024-b393ea2u	83	2	analysis	analysis	NN
cord-253024-b393ea2u	83	3	and	and	CC
cord-253024-b393ea2u	83	4	establishing	establish	VBG
cord-253024-b393ea2u	83	5	a	a	DT
cord-253024-b393ea2u	83	6	genetic	genetic	JJ
cord-253024-b393ea2u	83	7	recombination	recombination	NN
cord-253024-b393ea2u	83	8	map	map	NN
cord-253024-b393ea2u	83	9	of	of	IN
cord-253024-b393ea2u	83	10	the	the	DT
cord-253024-b393ea2u	83	11	group	group	NN
cord-253024-b393ea2u	83	12	F	F	NNP
cord-253024-b393ea2u	83	13	RNA+	RNA+	NNP
cord-253024-b393ea2u	83	14	mutants	mutant	NNS
cord-253024-b393ea2u	84	1	Recombination	recombination	NN
cord-253024-b393ea2u	84	2	analyses	analysis	NNS
cord-253024-b393ea2u	84	3	were	be	VBD
cord-253024-b393ea2u	84	4	performed	perform	VBN
cord-253024-b393ea2u	84	5	as	as	IN
cord-253024-b393ea2u	84	6	previously	previously	RB
cord-253024-b393ea2u	84	7	described	describe	VBN
cord-253024-b393ea2u	84	8	and	and	CC
cord-253024-b393ea2u	84	9	the	the	DT
cord-253024-b393ea2u	84	10	recombination	recombination	NN
cord-253024-b393ea2u	84	11	frequencies	frequency	NNS
cord-253024-b393ea2u	84	12	calculated	calculate	VBN
cord-253024-b393ea2u	84	13	as	as	IN
cord-253024-b393ea2u	84	14	the	the	DT
cord-253024-b393ea2u	84	15	percentage	percentage	NN
cord-253024-b393ea2u	84	16	of	of	IN
cord-253024-b393ea2u	84	17	ts+	ts+	NNP
cord-253024-b393ea2u	84	18	virus	virus	NN
cord-253024-b393ea2u	84	19	present	present	JJ
cord-253024-b393ea2u	84	20	in	in	IN
cord-253024-b393ea2u	84	21	the	the	DT
cord-253024-b393ea2u	84	22	progeny	progeny	NN
cord-253024-b393ea2u	84	23	from	from	IN
cord-253024-b393ea2u	84	24	the	the	DT
cord-253024-b393ea2u	84	25	following	following	JJ
cord-253024-b393ea2u	84	26	formula	formula	NN
cord-253024-b393ea2u	84	27	:	:	:
cord-253024-b393ea2u	84	28	RF	rf	NN
cord-253024-b393ea2u	84	29	=	=	SYM
cord-253024-b393ea2u	84	30	VW	VW	NNP
cord-253024-b393ea2u	84	31	,	,	,
cord-253024-b393ea2u	84	32	,	,	,
cord-253024-b393ea2u	84	33	.	.	.
cord-253024-b393ea2u	85	1	,	,	,
cord-253024-b393ea2u	86	1	-tA	-tA	:
cord-253024-b393ea2u	87	1	+	+	CC
cord-253024-b393ea2u	88	1	B)3~.s	B)3~.s	NNP
cord-253024-b393ea2u	88	2	x	x	NNP
cord-253024-b393ea2u	88	3	,	,	,
cord-253024-b393ea2u	88	4	ooo	ooo	UH
cord-253024-b393ea2u	88	5	,	,	,
cord-253024-b393ea2u	88	6	o	o	UH
cord-253024-b393ea2u	88	7	,	,	,
cord-253024-b393ea2u	88	8	.	.	.
cord-253024-b393ea2u	89	1	.	.	.
cord-253024-b393ea2u	90	1	-	-	:
cord-253024-b393ea2u	91	1	*	*	NFP
cord-253024-b393ea2u	91	2	bw32	bw32	NNP
cord-253024-b393ea2u	91	3	(	(	-LRB-
cord-253024-b393ea2u	91	4	AB	AB	NNP
cord-253024-b393ea2u	91	5	)	)	-RRB-
cord-253024-b393ea2u	91	6	,	,	,
cord-253024-b393ea2u	91	7	,	,	,
cord-253024-b393ea2u	91	8	.	.	.
cord-253024-b393ea2u	92	1	,	,	,
cord-253024-b393ea2u	92	2	was	be	VBD
cord-253024-b393ea2u	92	3	the	the	DT
cord-253024-b393ea2u	92	4	titer	titer	NN
cord-253024-b393ea2u	92	5	of	of	IN
cord-253024-b393ea2u	92	6	the	the	DT
cord-253024-b393ea2u	92	7	cross	cross	NN
cord-253024-b393ea2u	92	8	at	at	IN
cord-253024-b393ea2u	92	9	nonpermissive	nonpermissive	JJ
cord-253024-b393ea2u	92	10	temperature	temperature	NN
cord-253024-b393ea2u	92	11	while	while	IN
cord-253024-b393ea2u	92	12	(	(	-LRB-
cord-253024-b393ea2u	92	13	AB	AB	NNP
cord-253024-b393ea2u	92	14	)	)	-RRB-
cord-253024-b393ea2u	92	15	,	,	,
cord-253024-b393ea2u	92	16	,	,	,
cord-253024-b393ea2u	92	17	represented	represent	VBD
cord-253024-b393ea2u	92	18	the	the	DT
cord-253024-b393ea2u	92	19	titer	titer	NN
cord-253024-b393ea2u	92	20	of	of	IN
cord-253024-b393ea2u	92	21	the	the	DT
cord-253024-b393ea2u	92	22	same	same	JJ
cord-253024-b393ea2u	92	23	cross	cross	NN
cord-253024-b393ea2u	92	24	at	at	IN
cord-253024-b393ea2u	92	25	permissive	permissive	JJ
cord-253024-b393ea2u	92	26	temperature	temperature	NN
cord-253024-b393ea2u	92	27	.	.	.
cord-253024-b393ea2u	93	1	(	(	-LRB-
cord-253024-b393ea2u	93	2	A	a	NN
cord-253024-b393ea2u	93	3	+	+	CC
cord-253024-b393ea2u	93	4	B)39.5	B)39.5	NNP
cord-253024-b393ea2u	93	5	was	be	VBD
cord-253024-b393ea2u	93	6	the	the	DT
cord-253024-b393ea2u	93	7	sum	sum	NN
cord-253024-b393ea2u	93	8	of	of	IN
cord-253024-b393ea2u	93	9	the	the	DT
cord-253024-b393ea2u	93	10	revertants	revertant	NNS
cord-253024-b393ea2u	93	11	of	of	IN
cord-253024-b393ea2u	93	12	each	each	DT
cord-253024-b393ea2u	93	13	parent	parent	NN
cord-253024-b393ea2u	93	14	strain	strain	NN
cord-253024-b393ea2u	93	15	assayed	assay	VBN
cord-253024-b393ea2u	93	16	at	at	IN
cord-253024-b393ea2u	93	17	the	the	DT
cord-253024-b393ea2u	93	18	nonpermissive	nonpermissive	JJ
cord-253024-b393ea2u	93	19	temperature	temperature	NN
cord-253024-b393ea2u	93	20	.	.	.
cord-253024-b393ea2u	94	1	The	the	DT
cord-253024-b393ea2u	94	2	formula	formula	NN
cord-253024-b393ea2u	94	3	only	only	RB
cord-253024-b393ea2u	94	4	measured	measure	VBD
cord-253024-b393ea2u	94	5	single	single	JJ
cord-253024-b393ea2u	94	6	or	or	CC
cord-253024-b393ea2u	94	7	odd	odd	JJ
cord-253024-b393ea2u	94	8	-	-	HYPH
cord-253024-b393ea2u	94	9	number	number	NN
cord-253024-b393ea2u	94	10	cross	cross	JJ
cord-253024-b393ea2u	94	11	-	-	HYPH
cord-253024-b393ea2u	94	12	over	over	RP
cord-253024-b393ea2u	94	13	events	event	NNS
cord-253024-b393ea2u	94	14	resulting	result	VBG
cord-253024-b393ea2u	94	15	in	in	IN
cord-253024-b393ea2u	94	16	the	the	DT
cord-253024-b393ea2u	94	17	ts+	ts+	XX
cord-253024-b393ea2u	94	18	phenotype	phenotype	NN
cord-253024-b393ea2u	94	19	and	and	CC
cord-253024-b393ea2u	94	20	did	do	VBD
cord-253024-b393ea2u	94	21	not	not	RB
cord-253024-b393ea2u	94	22	account	account	VB
cord-253024-b393ea2u	94	23	for	for	IN
cord-253024-b393ea2u	94	24	recombination	recombination	NN
cord-253024-b393ea2u	94	25	events	event	NNS
cord-253024-b393ea2u	94	26	that	that	WDT
cord-253024-b393ea2u	94	27	resulted	result	VBD
cord-253024-b393ea2u	94	28	in	in	IN
cord-253024-b393ea2u	94	29	the	the	DT
cord-253024-b393ea2u	94	30	doublets	doublet	NNS
cord-253024-b393ea2u	94	31	mutant	mutant	JJ
cord-253024-b393ea2u	94	32	phenotype	phenotype	NN
cord-253024-b393ea2u	94	33	.	.	.
cord-253024-b393ea2u	95	1	All	all	DT
cord-253024-b393ea2u	95	2	recombination	recombination	NN
cord-253024-b393ea2u	95	3	frequencies	frequency	NNS
cord-253024-b393ea2u	95	4	were	be	VBD
cord-253024-b393ea2u	95	5	standardized	standardize	VBN
cord-253024-b393ea2u	95	6	to	to	IN
cord-253024-b393ea2u	95	7	a	a	DT
cord-253024-b393ea2u	95	8	standard	standard	JJ
cord-253024-b393ea2u	95	9	cross	cross	NN
cord-253024-b393ea2u	95	10	[	[	-LRB-
cord-253024-b393ea2u	95	11	LA7	LA7	NNP
cord-253024-b393ea2u	95	12	X	X	NNP
cord-253024-b393ea2u	95	13	NC1	NC1	NNP
cord-253024-b393ea2u	95	14	6	6	CD
cord-253024-b393ea2u	95	15	;	;	:
cord-253024-b393ea2u	95	16	recombination	recombination	NN
cord-253024-b393ea2u	95	17	frequency	frequency	NN
cord-253024-b393ea2u	95	18	3.0	3.0	CD
cord-253024-b393ea2u	95	19	f	f	CD
cord-253024-b393ea2u	95	20	1.21	1.21	CD
cord-253024-b393ea2u	95	21	as	as	IN
cord-253024-b393ea2u	95	22	previously	previously	RB
cord-253024-b393ea2u	95	23	described	describe	VBN
cord-253024-b393ea2u	95	24	.	.	.
cord-253024-b393ea2u	96	1	The	the	DT
cord-253024-b393ea2u	96	2	mutants	mutant	NNS
cord-253024-b393ea2u	96	3	were	be	VBD
cord-253024-b393ea2u	96	4	aligned	align	VBN
cord-253024-b393ea2u	96	5	according	accord	VBG
cord-253024-b393ea2u	96	6	to	to	IN
cord-253024-b393ea2u	96	7	their	-PRON-	PRP$
cord-253024-b393ea2u	96	8	standardized	standardized	JJ
cord-253024-b393ea2u	96	9	recombination	recombination	NN
cord-253024-b393ea2u	96	10	frequencies	frequency	NNS
cord-253024-b393ea2u	96	11	and	and	CC
cord-253024-b393ea2u	96	12	positioned	position	VBD
cord-253024-b393ea2u	96	13	from	from	IN
cord-253024-b393ea2u	96	14	LA7	LA7	NNP
cord-253024-b393ea2u	96	15	,	,	,
cord-253024-b393ea2u	96	16	LA1	LA1	NNP
cord-253024-b393ea2u	96	17	8	8	CD
cord-253024-b393ea2u	96	18	,	,	,
cord-253024-b393ea2u	96	19	and	and	CC
cord-253024-b393ea2u	96	20	NC1	NC1	NNP
cord-253024-b393ea2u	96	21	6	6	CD
cord-253024-b393ea2u	96	22	.	.	.
cord-253024-b393ea2u	97	1	Cultures	culture	NNS
cord-253024-b393ea2u	97	2	of	of	IN
cord-253024-b393ea2u	97	3	DBT	DBT	NNP
cord-253024-b393ea2u	97	4	cells	cell	NNS
cord-253024-b393ea2u	97	5	in	in	IN
cord-253024-b393ea2u	97	6	lOO	lOO	NNP
cord-253024-b393ea2u	97	7	-	-	HYPH
cord-253024-b393ea2u	97	8	mm2	mm2	NNS
cord-253024-b393ea2u	97	9	dishes	dish	NNS
cord-253024-b393ea2u	97	10	were	be	VBD
cord-253024-b393ea2u	97	11	infected	infect	VBN
cord-253024-b393ea2u	97	12	at	at	IN
cord-253024-b393ea2u	97	13	a	a	DT
cord-253024-b393ea2u	97	14	m.o.i	m.o.i	NN
cord-253024-b393ea2u	97	15	.	.	NN
cord-253024-b393ea2u	97	16	of	of	IN
cord-253024-b393ea2u	97	17	5	5	CD
cord-253024-b393ea2u	97	18	with	with	IN
cord-253024-b393ea2u	97	19	different	different	JJ
cord-253024-b393ea2u	97	20	ts	ts	NNP
cord-253024-b393ea2u	97	21	mutants	mutant	NNS
cord-253024-b393ea2u	97	22	,	,	,
cord-253024-b393ea2u	97	23	revertant	revertant	NN
cord-253024-b393ea2u	97	24	viruses	virus	NNS
cord-253024-b393ea2u	97	25	,	,	,
cord-253024-b393ea2u	97	26	or	or	CC
cord-253024-b393ea2u	97	27	recombinant	recombinant	JJ
cord-253024-b393ea2u	97	28	viruses	virus	NNS
cord-253024-b393ea2u	97	29	,	,	,
cord-253024-b393ea2u	97	30	and	and	CC
cord-253024-b393ea2u	97	31	intercellular	intercellular	JJ
cord-253024-b393ea2u	97	32	RNA	RNA	NNP
cord-253024-b393ea2u	97	33	was	be	VBD
cord-253024-b393ea2u	97	34	harvested	harvest	VBN
cord-253024-b393ea2u	97	35	at	at	IN
cord-253024-b393ea2u	97	36	8-l	8-l	CD
cord-253024-b393ea2u	97	37	2	2	CD
cord-253024-b393ea2u	97	38	hr	hr	NN
cord-253024-b393ea2u	97	39	postinfection	postinfection	NN
cord-253024-b393ea2u	97	40	.	.	.
cord-253024-b393ea2u	98	1	The	the	DT
cord-253024-b393ea2u	98	2	precipitate	precipitate	NN
cord-253024-b393ea2u	98	3	was	be	VBD
cord-253024-b393ea2u	98	4	resuspended	resuspend	VBN
cord-253024-b393ea2u	98	5	in	in	IN
cord-253024-b393ea2u	98	6	TE	TE	NNP
cord-253024-b393ea2u	98	7	buffer	buffer	NN
cord-253024-b393ea2u	98	8	(	(	-LRB-
cord-253024-b393ea2u	98	9	10	10	CD
cord-253024-b393ea2u	98	10	mNI	mNI	NNP
cord-253024-b393ea2u	98	11	Tris	Tris	NNP
cord-253024-b393ea2u	98	12	-	-	HYPH
cord-253024-b393ea2u	98	13	HCI	HCI	NNP
cord-253024-b393ea2u	98	14	,	,	,
cord-253024-b393ea2u	98	15	pH	pH	NNP
cord-253024-b393ea2u	98	16	7.2	7.2	CD
cord-253024-b393ea2u	98	17	,	,	,
cord-253024-b393ea2u	98	18	1	1	CD
cord-253024-b393ea2u	98	19	mM	mm	CD
cord-253024-b393ea2u	98	20	EDTA	EDTA	NNP
cord-253024-b393ea2u	98	21	)	)	-RRB-
cord-253024-b393ea2u	98	22	and	and	CC
cord-253024-b393ea2u	98	23	used	use	VBD
cord-253024-b393ea2u	98	24	as	as	IN
cord-253024-b393ea2u	98	25	template	template	NN
cord-253024-b393ea2u	98	26	for	for	IN
cord-253024-b393ea2u	98	27	reverse	reverse	JJ
cord-253024-b393ea2u	98	28	transcription	transcription	NN
cord-253024-b393ea2u	98	29	and	and	CC
cord-253024-b393ea2u	98	30	TAQ	taq	NN
cord-253024-b393ea2u	98	31	amplification	amplification	NN
cord-253024-b393ea2u	98	32	reactions	reaction	NNS
cord-253024-b393ea2u	98	33	.	.	.
cord-253024-b393ea2u	99	1	cDNA	cDNA	NNP
cord-253024-b393ea2u	99	2	synthesis	synthesis	NNP
cord-253024-b393ea2u	99	3	was	be	VBD
cord-253024-b393ea2u	99	4	carried	carry	VBN
cord-253024-b393ea2u	99	5	out	out	RP
cord-253024-b393ea2u	99	6	in	in	IN
cord-253024-b393ea2u	99	7	a	a	DT
cord-253024-b393ea2u	99	8	20-~1	20-~1	CD
cord-253024-b393ea2u	99	9	reaction	reaction	NN
cord-253024-b393ea2u	99	10	mixture	mixture	NN
cord-253024-b393ea2u	99	11	containing	contain	VBG
cord-253024-b393ea2u	99	12	1	1	CD
cord-253024-b393ea2u	99	13	pg	pg	NN
cord-253024-b393ea2u	99	14	intercellular	intercellular	JJ
cord-253024-b393ea2u	99	15	RNA	RNA	NNP
cord-253024-b393ea2u	99	16	,	,	,
cord-253024-b393ea2u	99	17	5	5	CD
cord-253024-b393ea2u	99	18	mM	mm	CD
cord-253024-b393ea2u	99	19	MgCI	MgCI	NNP
cord-253024-b393ea2u	99	20	,	,	,
cord-253024-b393ea2u	99	21	,	,	,
cord-253024-b393ea2u	99	22	10	10	CD
cord-253024-b393ea2u	99	23	m	m	NN
cord-253024-b393ea2u	99	24	/	/	SYM
cord-253024-b393ea2u	99	25	l	l	NN
cord-253024-b393ea2u	99	26	/	/	SYM
cord-253024-b393ea2u	99	27	l	l	NNP
cord-253024-b393ea2u	99	28	Tris	Tris	NNP
cord-253024-b393ea2u	99	29	-	-	HYPH
cord-253024-b393ea2u	99	30	HCI	HCI	NNP
cord-253024-b393ea2u	99	31	pH	pH	NNP
cord-253024-b393ea2u	99	32	8.4	8.4	CD
cord-253024-b393ea2u	99	33	,	,	,
cord-253024-b393ea2u	99	34	50	50	CD
cord-253024-b393ea2u	99	35	mM	mm	CD
cord-253024-b393ea2u	99	36	KCI	KCI	NNP
cord-253024-b393ea2u	99	37	,	,	,
cord-253024-b393ea2u	99	38	0.1	0.1	CD
cord-253024-b393ea2u	99	39	%	%	NN
cord-253024-b393ea2u	99	40	Triton	Triton	NNP
cord-253024-b393ea2u	99	41	X	X	NNP
cord-253024-b393ea2u	99	42	-	-	HYPH
cord-253024-b393ea2u	99	43	l	l	NN
cord-253024-b393ea2u	99	44	00	00	CD
cord-253024-b393ea2u	99	45	(	(	-LRB-
cord-253024-b393ea2u	99	46	Sigma	Sigma	NNP
cord-253024-b393ea2u	99	47	)	)	-RRB-
cord-253024-b393ea2u	99	48	,	,	,
cord-253024-b393ea2u	99	49	1	1	CD
cord-253024-b393ea2u	99	50	mM	mm	CD
cord-253024-b393ea2u	99	51	dNTP	dntp	NN
cord-253024-b393ea2u	99	52	each	each	DT
cord-253024-b393ea2u	99	53	(	(	-LRB-
cord-253024-b393ea2u	99	54	Promega	Promega	NNP
cord-253024-b393ea2u	99	55	)	)	-RRB-
cord-253024-b393ea2u	99	56	,	,	,
cord-253024-b393ea2u	99	57	0.5	0.5	CD
cord-253024-b393ea2u	99	58	pg	pg	NN
cord-253024-b393ea2u	99	59	random	random	NNP
cord-253024-b393ea2u	99	60	hexamer	hexamer	NNP
cord-253024-b393ea2u	99	61	(	(	-LRB-
cord-253024-b393ea2u	99	62	Perkin	Perkin	NNP
cord-253024-b393ea2u	99	63	Elmer	Elmer	NNP
cord-253024-b393ea2u	99	64	Cetus	Cetus	NNP
cord-253024-b393ea2u	99	65	)	)	-RRB-
cord-253024-b393ea2u	99	66	,	,	,
cord-253024-b393ea2u	99	67	1	1	CD
cord-253024-b393ea2u	99	68	U	u	NN
cord-253024-b393ea2u	99	69	/	/	SYM
cord-253024-b393ea2u	99	70	PI	PI	NNP
cord-253024-b393ea2u	99	71	RNasin	RNasin	NNP
cord-253024-b393ea2u	99	72	(	(	-LRB-
cord-253024-b393ea2u	99	73	Promega	Promega	NNP
cord-253024-b393ea2u	99	74	)	)	-RRB-
cord-253024-b393ea2u	99	75	,	,	,
cord-253024-b393ea2u	99	76	15	15	CD
cord-253024-b393ea2u	99	77	U	U	NNP
cord-253024-b393ea2u	99	78	AMV	AMV	NNP
cord-253024-b393ea2u	99	79	reverse	reverse	NN
cord-253024-b393ea2u	99	80	transcriptase	transcriptase	NN
cord-253024-b393ea2u	99	81	(	(	-LRB-
cord-253024-b393ea2u	99	82	Promega	Promega	NNP
cord-253024-b393ea2u	99	83	)	)	-RRB-
cord-253024-b393ea2u	99	84	and	and	CC
cord-253024-b393ea2u	99	85	incubated	incubate	VBD
cord-253024-b393ea2u	99	86	at	at	IN
cord-253024-b393ea2u	99	87	42	42	CD
cord-253024-b393ea2u	99	88	"	"	``
cord-253024-b393ea2u	99	89	for	for	IN
cord-253024-b393ea2u	99	90	1	1	CD
cord-253024-b393ea2u	99	91	hr	hr	NN
cord-253024-b393ea2u	99	92	.	.	.
cord-253024-b393ea2u	100	1	The	the	DT
cord-253024-b393ea2u	100	2	products	product	NNS
cord-253024-b393ea2u	100	3	were	be	VBD
cord-253024-b393ea2u	100	4	extracted	extract	VBN
cord-253024-b393ea2u	100	5	and	and	CC
cord-253024-b393ea2u	100	6	precipitated	precipitate	VBN
cord-253024-b393ea2u	100	7	in	in	IN
cord-253024-b393ea2u	100	8	ethanol	ethanol	NN
cord-253024-b393ea2u	100	9	and	and	CC
cord-253024-b393ea2u	100	10	resuspended	resuspend	VBD
cord-253024-b393ea2u	100	11	in	in	IN
cord-253024-b393ea2u	100	12	50	50	CD
cord-253024-b393ea2u	101	1	~1	~1	DT
cord-253024-b393ea2u	101	2	H	H	NNP
cord-253024-b393ea2u	101	3	,	,	,
cord-253024-b393ea2u	101	4	O	o	UH
cord-253024-b393ea2u	101	5	prior	prior	RB
cord-253024-b393ea2u	101	6	to	to	IN
cord-253024-b393ea2u	101	7	successive	successive	JJ
cord-253024-b393ea2u	101	8	rounds	round	NNS
cord-253024-b393ea2u	101	9	of	of	IN
cord-253024-b393ea2u	101	10	polymerase	polymerase	NN
cord-253024-b393ea2u	101	11	chain	chain	NN
cord-253024-b393ea2u	101	12	amplification	amplification	NN
cord-253024-b393ea2u	101	13	(	(	-LRB-
cord-253024-b393ea2u	101	14	PCR	PCR	NNP
cord-253024-b393ea2u	101	15	)	)	-RRB-
cord-253024-b393ea2u	101	16	.	.	.
cord-253024-b393ea2u	102	1	One	one	CD
cord-253024-b393ea2u	102	2	-	-	HYPH
cord-253024-b393ea2u	102	3	tenth	tenth	NN
cord-253024-b393ea2u	102	4	of	of	IN
cord-253024-b393ea2u	102	5	the	the	DT
cord-253024-b393ea2u	102	6	cDNA	cdna	NN
cord-253024-b393ea2u	102	7	was	be	VBD
cord-253024-b393ea2u	102	8	amplified	amplify	VBN
cord-253024-b393ea2u	102	9	in	in	IN
cord-253024-b393ea2u	102	10	a	a	DT
cord-253024-b393ea2u	102	11	50	50	CD
cord-253024-b393ea2u	102	12	~1	~1	DT
cord-253024-b393ea2u	102	13	reaction	reaction	NN
cord-253024-b393ea2u	102	14	mixture	mixture	NN
cord-253024-b393ea2u	102	15	containing	contain	VBG
cord-253024-b393ea2u	102	16	10	10	CD
cord-253024-b393ea2u	102	17	mM	mM	NNP
cord-253024-b393ea2u	102	18	Tris	Tris	NNP
cord-253024-b393ea2u	102	19	-	-	HYPH
cord-253024-b393ea2u	102	20	HCI	HCI	NNP
cord-253024-b393ea2u	102	21	(	(	-LRB-
cord-253024-b393ea2u	102	22	pH	pH	NNP
cord-253024-b393ea2u	102	23	9.0	9.0	CD
cord-253024-b393ea2u	102	24	)	)	-RRB-
cord-253024-b393ea2u	102	25	50	50	CD
cord-253024-b393ea2u	102	26	mM	mm	CD
cord-253024-b393ea2u	102	27	KCI	KCI	NNP
cord-253024-b393ea2u	102	28	,	,	,
cord-253024-b393ea2u	102	29	3	3	CD
cord-253024-b393ea2u	102	30	mM	mm	CD
cord-253024-b393ea2u	102	31	MgCI	MgCI	NNP
cord-253024-b393ea2u	102	32	,	,	,
cord-253024-b393ea2u	102	33	,	,	,
cord-253024-b393ea2u	102	34	0.1	0.1	CD
cord-253024-b393ea2u	102	35	O	o	UH
cord-253024-b393ea2u	102	36	/	/	SYM
cord-253024-b393ea2u	102	37	O	o	UH
cord-253024-b393ea2u	102	38	Triton	Triton	NNP
cord-253024-b393ea2u	102	39	X	X	NNP
cord-253024-b393ea2u	102	40	-	-	HYPH
cord-253024-b393ea2u	102	41	l	l	NN
cord-253024-b393ea2u	102	42	00	00	CD
cord-253024-b393ea2u	102	43	,	,	,
cord-253024-b393ea2u	102	44	1.25	1.25	CD
cord-253024-b393ea2u	102	45	U	U	NNP
cord-253024-b393ea2u	102	46	TAQ	taq	NN
cord-253024-b393ea2u	102	47	DNA	dna	NN
cord-253024-b393ea2u	102	48	polymerase	polymerase	NN
cord-253024-b393ea2u	102	49	(	(	-LRB-
cord-253024-b393ea2u	102	50	Promega	Promega	NNP
cord-253024-b393ea2u	102	51	)	)	-RRB-
cord-253024-b393ea2u	102	52	,	,	,
cord-253024-b393ea2u	102	53	0.2	0.2	CD
cord-253024-b393ea2u	102	54	mM	mm	CD
cord-253024-b393ea2u	102	55	dNTP	dntp	NN
cord-253024-b393ea2u	102	56	mixture	mixture	NN
cord-253024-b393ea2u	102	57	and	and	CC
cord-253024-b393ea2u	102	58	the	the	DT
cord-253024-b393ea2u	102	59	appropriate	appropriate	JJ
cord-253024-b393ea2u	102	60	primer	primer	NN
cord-253024-b393ea2u	102	61	pairs	pair	NNS
cord-253024-b393ea2u	102	62	(	(	-LRB-
cord-253024-b393ea2u	102	63	100	100	CD
cord-253024-b393ea2u	102	64	ng	ng	NN
cord-253024-b393ea2u	102	65	of	of	IN
cord-253024-b393ea2u	102	66	each	each	DT
cord-253024-b393ea2u	102	67	)	)	-RRB-
cord-253024-b393ea2u	102	68	.	.	.
cord-253024-b393ea2u	103	1	PCR	PCR	NNP
cord-253024-b393ea2u	103	2	amplification	amplification	NN
cord-253024-b393ea2u	103	3	was	be	VBD
cord-253024-b393ea2u	103	4	performed	perform	VBN
cord-253024-b393ea2u	103	5	for	for	IN
cord-253024-b393ea2u	103	6	30	30	CD
cord-253024-b393ea2u	103	7	successive	successive	JJ
cord-253024-b393ea2u	103	8	cycles	cycle	NNS
cord-253024-b393ea2u	103	9	at	at	IN
cord-253024-b393ea2u	103	10	1.5	1.5	CD
cord-253024-b393ea2u	103	11	min	min	NN
cord-253024-b393ea2u	103	12	at	at	IN
cord-253024-b393ea2u	103	13	94	94	CD
cord-253024-b393ea2u	103	14	"	"	``
cord-253024-b393ea2u	103	15	to	to	TO
cord-253024-b393ea2u	103	16	denature	denature	VB
cord-253024-b393ea2u	103	17	the	the	DT
cord-253024-b393ea2u	103	18	DNA	dna	NN
cord-253024-b393ea2u	103	19	,	,	,
cord-253024-b393ea2u	103	20	2	2	CD
cord-253024-b393ea2u	103	21	min	min	NN
cord-253024-b393ea2u	103	22	at	at	IN
cord-253024-b393ea2u	103	23	55	55	CD
cord-253024-b393ea2u	103	24	"	"	``
cord-253024-b393ea2u	103	25	for	for	IN
cord-253024-b393ea2u	103	26	primer	primer	NN
cord-253024-b393ea2u	103	27	annealing	annealing	NN
cord-253024-b393ea2u	103	28	,	,	,
cord-253024-b393ea2u	103	29	and	and	CC
cord-253024-b393ea2u	103	30	4	4	CD
cord-253024-b393ea2u	103	31	min	min	NN
cord-253024-b393ea2u	103	32	at	at	IN
cord-253024-b393ea2u	103	33	72	72	CD
cord-253024-b393ea2u	103	34	"	"	``
cord-253024-b393ea2u	103	35	for	for	IN
cord-253024-b393ea2u	103	36	primer	primer	NN
cord-253024-b393ea2u	103	37	extension	extension	NN
cord-253024-b393ea2u	103	38	.	.	.
cord-253024-b393ea2u	104	1	The	the	DT
cord-253024-b393ea2u	104	2	products	product	NNS
cord-253024-b393ea2u	104	3	were	be	VBD
cord-253024-b393ea2u	104	4	loaded	load	VBN
cord-253024-b393ea2u	104	5	onto	onto	IN
cord-253024-b393ea2u	104	6	1	1	CD
cord-253024-b393ea2u	104	7	%	%	NN
cord-253024-b393ea2u	104	8	agarose	agarose	JJ
cord-253024-b393ea2u	104	9	gels	gel	NNS
cord-253024-b393ea2u	104	10	in	in	IN
cord-253024-b393ea2u	104	11	TAE	TAE	NNP
cord-253024-b393ea2u	104	12	(	(	-LRB-
cord-253024-b393ea2u	104	13	40	40	CD
cord-253024-b393ea2u	104	14	mltlTris	mltlTris	NNP
cord-253024-b393ea2u	104	15	,	,	,
cord-253024-b393ea2u	104	16	9	9	CD
cord-253024-b393ea2u	104	17	mM	mm	CD
cord-253024-b393ea2u	104	18	NaCI	naci	NN
cord-253024-b393ea2u	104	19	,	,	,
cord-253024-b393ea2u	104	20	1	1	CD
cord-253024-b393ea2u	104	21	mM	mm	CD
cord-253024-b393ea2u	104	22	EDTA	EDTA	NNP
cord-253024-b393ea2u	104	23	,	,	,
cord-253024-b393ea2u	104	24	(	(	-LRB-
cord-253024-b393ea2u	104	25	pH	pH	NNP
cord-253024-b393ea2u	104	26	8.0	8.0	CD
cord-253024-b393ea2u	104	27	)	)	-RRB-
cord-253024-b393ea2u	104	28	)	)	-RRB-
cord-253024-b393ea2u	104	29	separated	separate	VBD
cord-253024-b393ea2u	104	30	electrophoretically	electrophoretically	RB
cord-253024-b393ea2u	104	31	,	,	,
cord-253024-b393ea2u	104	32	stained	stain	VBN
cord-253024-b393ea2u	104	33	with	with	IN
cord-253024-b393ea2u	104	34	ethidium	ethidium	NN
cord-253024-b393ea2u	104	35	bromide	bromide	NN
cord-253024-b393ea2u	104	36	,	,	,
cord-253024-b393ea2u	104	37	and	and	CC
cord-253024-b393ea2u	104	38	visualized	visualize	VBN
cord-253024-b393ea2u	104	39	under	under	IN
cord-253024-b393ea2u	104	40	UV	uv	NN
cord-253024-b393ea2u	104	41	light	light	NN
cord-253024-b393ea2u	104	42	.	.	.
cord-253024-b393ea2u	105	1	The	the	DT
cord-253024-b393ea2u	105	2	DNA	dna	NN
cord-253024-b393ea2u	105	3	products	product	NNS
cord-253024-b393ea2u	105	4	were	be	VBD
cord-253024-b393ea2u	105	5	transferred	transfer	VBN
cord-253024-b393ea2u	105	6	to	to	IN
cord-253024-b393ea2u	105	7	nitrocellulose	nitrocellulose	NN
cord-253024-b393ea2u	105	8	filters	filter	NNS
cord-253024-b393ea2u	105	9	,	,	,
cord-253024-b393ea2u	105	10	and	and	CC
cord-253024-b393ea2u	105	11	confirmed	confirm	VBN
cord-253024-b393ea2u	105	12	by	by	IN
cord-253024-b393ea2u	105	13	hybridization	hybridization	NN
cord-253024-b393ea2u	105	14	with	with	IN
cord-253024-b393ea2u	105	15	32P	32p	CD
cord-253024-b393ea2u	105	16	-	-	HYPH
cord-253024-b393ea2u	105	17	labeled	label	VBN
cord-253024-b393ea2u	105	18	oligomer	oligomer	NN
cord-253024-b393ea2u	105	19	probes	probe	NNS
cord-253024-b393ea2u	105	20	specific	specific	JJ
cord-253024-b393ea2u	105	21	for	for	IN
cord-253024-b393ea2u	105	22	internal	internal	JJ
cord-253024-b393ea2u	105	23	sequences	sequence	NNS
cord-253024-b393ea2u	105	24	in	in	IN
cord-253024-b393ea2u	105	25	each	each	DT
cord-253024-b393ea2u	105	26	gene	gene	NN
cord-253024-b393ea2u	105	27	fragment	fragment	NN
cord-253024-b393ea2u	105	28	amplified	amplify	VBD
cord-253024-b393ea2u	105	29	.	.	.
cord-253024-b393ea2u	106	1	Three	three	CD
cord-253024-b393ea2u	106	2	sets	set	NNS
cord-253024-b393ea2u	106	3	of	of	IN
cord-253024-b393ea2u	106	4	overlapping	overlap	VBG
cord-253024-b393ea2u	106	5	primer	primer	NN
cord-253024-b393ea2u	106	6	pairs	pair	NNS
cord-253024-b393ea2u	106	7	were	be	VBD
cord-253024-b393ea2u	106	8	obtained	obtain	VBN
cord-253024-b393ea2u	106	9	from	from	IN
cord-253024-b393ea2u	106	10	highly	highly	RB
cord-253024-b393ea2u	106	11	conserved	conserved	JJ
cord-253024-b393ea2u	106	12	sequences	sequence	NNS
cord-253024-b393ea2u	106	13	in	in	IN
cord-253024-b393ea2u	106	14	the	the	DT
cord-253024-b393ea2u	106	15	MHV	MHV	NNP
cord-253024-b393ea2u	106	16	-	-	HYPH
cord-253024-b393ea2u	106	17	A59	A59	NNP
cord-253024-b393ea2u	106	18	and	and	CC
cord-253024-b393ea2u	106	19	JHM	JHM	NNP
cord-253024-b393ea2u	106	20	S	S	NNP
cord-253024-b393ea2u	106	21	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	106	22	genes	gene	NNS
cord-253024-b393ea2u	106	23	(	(	-LRB-
cord-253024-b393ea2u	106	24	Banner	Banner	NNP
cord-253024-b393ea2u	106	25	et	et	NNP
cord-253024-b393ea2u	106	26	a/.	a/.	NNP
cord-253024-b393ea2u	106	27	,	,	,
cord-253024-b393ea2u	106	28	1990	1990	CD
cord-253024-b393ea2u	106	29	;	;	:
cord-253024-b393ea2u	107	1	Luytjes	Luytjes	NNP
cord-253024-b393ea2u	107	2	et	et	NNP
cord-253024-b393ea2u	107	3	al	al	NNP
cord-253024-b393ea2u	107	4	.	.	NNP
cord-253024-b393ea2u	107	5	,	,	,
cord-253024-b393ea2u	107	6	1987	1987	CD
cord-253024-b393ea2u	107	7	;	;	:
cord-253024-b393ea2u	107	8	Parker	Parker	NNP
cord-253024-b393ea2u	107	9	et	et	NNP
cord-253024-b393ea2u	107	10	a/.	a/.	NNP
cord-253024-b393ea2u	107	11	,	,	,
cord-253024-b393ea2u	107	12	1990	1990	CD
cord-253024-b393ea2u	107	13	)	)	-RRB-
cord-253024-b393ea2u	107	14	.	.	.
cord-253024-b393ea2u	108	1	Set	Set	VBN
cord-253024-b393ea2u	109	1	A	a	DT
cord-253024-b393ea2u	109	2	was	be	VBD
cord-253024-b393ea2u	109	3	derived	derive	VBN
cord-253024-b393ea2u	109	4	from	from	IN
cord-253024-b393ea2u	109	5	sequences	sequence	NNS
cord-253024-b393ea2u	109	6	spanning	span	VBG
cord-253024-b393ea2u	109	7	nucleotides	nucleotide	NNS
cord-253024-b393ea2u	109	8	-1	-1	JJ
cord-253024-b393ea2u	109	9	to	to	IN
cord-253024-b393ea2u	109	10	15	15	CD
cord-253024-b393ea2u	109	11	(	(	-LRB-
cord-253024-b393ea2u	109	12	CAT	cat	NN
cord-253024-b393ea2u	109	13	GCT	GCT	NNP
cord-253024-b393ea2u	109	14	GGT	GGT	NNP
cord-253024-b393ea2u	109	15	CGT	CGT	NNP
cord-253024-b393ea2u	109	16	GTT	GTT	NNP
cord-253024-b393ea2u	109	17	T	T	NNP
cord-253024-b393ea2u	109	18	)	)	-RRB-
cord-253024-b393ea2u	109	19	and	and	CC
cord-253024-b393ea2u	109	20	662	662	CD
cord-253024-b393ea2u	109	21	-	-	SYM
cord-253024-b393ea2u	109	22	677	677	CD
cord-253024-b393ea2u	110	1	(	(	-LRB-
cord-253024-b393ea2u	110	2	AAC	AAC	NNP
cord-253024-b393ea2u	110	3	GTA	GTA	NNP
cord-253024-b393ea2u	110	4	GTA	GTA	NNP
cord-253024-b393ea2u	110	5	GCG	GCG	NNP
cord-253024-b393ea2u	110	6	GAG	gag	NN
cord-253024-b393ea2u	110	7	G	G	NNP
cord-253024-b393ea2u	110	8	)	)	-RRB-
cord-253024-b393ea2u	110	9	,	,	,
cord-253024-b393ea2u	110	10	respectively	respectively	RB
cord-253024-b393ea2u	110	11	,	,	,
cord-253024-b393ea2u	110	12	and	and	CC
cord-253024-b393ea2u	110	13	should	should	MD
cord-253024-b393ea2u	110	14	result	result	VB
cord-253024-b393ea2u	110	15	in	in	IN
cord-253024-b393ea2u	110	16	a	a	DT
cord-253024-b393ea2u	110	17	DNA	dna	NN
cord-253024-b393ea2u	110	18	fragment	fragment	NN
cord-253024-b393ea2u	110	19	of	of	IN
cord-253024-b393ea2u	110	20	0.67	0.67	CD
cord-253024-b393ea2u	110	21	kb	kb	NNP
cord-253024-b393ea2u	110	22	in	in	IN
cord-253024-b393ea2u	110	23	length	length	NN
cord-253024-b393ea2u	110	24	.	.	.
cord-253024-b393ea2u	111	1	Set	Set	NNP
cord-253024-b393ea2u	111	2	B	B	NNP
cord-253024-b393ea2u	111	3	was	be	VBD
cord-253024-b393ea2u	111	4	derived	derive	VBN
cord-253024-b393ea2u	111	5	from	from	IN
cord-253024-b393ea2u	111	6	nucleotides	nucleotide	NNS
cord-253024-b393ea2u	111	7	642	642	CD
cord-253024-b393ea2u	111	8	-	-	SYM
cord-253024-b393ea2u	111	9	662	662	CD
cord-253024-b393ea2u	112	1	(	(	-LRB-
cord-253024-b393ea2u	112	2	GCG	GCG	NNP
cord-253024-b393ea2u	112	3	TAC	TAC	NNP
cord-253024-b393ea2u	112	4	TAT	tat	NN
cord-253024-b393ea2u	112	5	TCG	TCG	NNP
cord-253024-b393ea2u	112	6	GAT	GAT	NNP
cord-253024-b393ea2u	112	7	AAA	AAA	NNP
cord-253024-b393ea2u	112	8	CC	CC	NNP
cord-253024-b393ea2u	112	9	)	)	-RRB-
cord-253024-b393ea2u	112	10	and	and	CC
cord-253024-b393ea2u	112	11	2	2	CD
cord-253024-b393ea2u	112	12	103	103	CD
cord-253024-b393ea2u	112	13	-	-	SYM
cord-253024-b393ea2u	112	14	2	2	CD
cord-253024-b393ea2u	112	15	115	115	CD
cord-253024-b393ea2u	112	16	(	(	-LRB-
cord-253024-b393ea2u	112	17	CCC	CCC	NNP
cord-253024-b393ea2u	112	18	ACG	ACG	NNP
cord-253024-b393ea2u	112	19	ACC	ACC	NNP
cord-253024-b393ea2u	112	20	GAA	GAA	NNP
cord-253024-b393ea2u	112	21	TAC	TAC	NNP
cord-253024-b393ea2u	112	22	G	G	NNP
cord-253024-b393ea2u	112	23	)	)	-RRB-
cord-253024-b393ea2u	112	24	and	and	CC
cord-253024-b393ea2u	112	25	were	be	VBD
cord-253024-b393ea2u	112	26	used	use	VBN
cord-253024-b393ea2u	112	27	to	to	TO
cord-253024-b393ea2u	112	28	amplify	amplify	VB
cord-253024-b393ea2u	112	29	a	a	DT
cord-253024-b393ea2u	112	30	-1.5-kb	-1.5-kb	CD
cord-253024-b393ea2u	112	31	fragment	fragment	NN
cord-253024-b393ea2u	112	32	encompassing	encompass	VBG
cord-253024-b393ea2u	112	33	the	the	DT
cord-253024-b393ea2u	112	34	hypervariable	hypervariable	JJ
cord-253024-b393ea2u	112	35	region	region	NN
cord-253024-b393ea2u	112	36	in	in	IN
cord-253024-b393ea2u	112	37	the	the	DT
cord-253024-b393ea2u	112	38	Sl	sl	NN
cord-253024-b393ea2u	112	39	domain	domain	NN
cord-253024-b393ea2u	113	1	(	(	-LRB-
cord-253024-b393ea2u	113	2	Banner	Banner	NNP
cord-253024-b393ea2u	113	3	et	et	NNP
cord-253024-b393ea2u	113	4	a/.	a/.	NNP
cord-253024-b393ea2u	113	5	,	,	,
cord-253024-b393ea2u	113	6	1990	1990	CD
cord-253024-b393ea2u	113	7	;	;	:
cord-253024-b393ea2u	113	8	Parker	Parker	NNP
cord-253024-b393ea2u	113	9	et	et	NNP
cord-253024-b393ea2u	113	10	a/.	a/.	NNP
cord-253024-b393ea2u	113	11	,	,	,
cord-253024-b393ea2u	113	12	1990	1990	CD
cord-253024-b393ea2u	113	13	)	)	-RRB-
cord-253024-b393ea2u	113	14	.	.	.
cord-253024-b393ea2u	114	1	Set	Set	NNP
cord-253024-b393ea2u	114	2	C	C	NNP
cord-253024-b393ea2u	114	3	was	be	VBD
cord-253024-b393ea2u	114	4	derived	derive	VBN
cord-253024-b393ea2u	114	5	from	from	IN
cord-253024-b393ea2u	114	6	nucleotides	nucleotide	NNS
cord-253024-b393ea2u	114	7	2067	2067	CD
cord-253024-b393ea2u	114	8	-	-	HYPH
cord-253024-b393ea2u	114	9	2083	2083	CD
cord-253024-b393ea2u	114	10	(	(	-LRB-
cord-253024-b393ea2u	114	11	ACG	ACG	NNP
cord-253024-b393ea2u	114	12	GAT	GAT	NNP
cord-253024-b393ea2u	114	13	GAG	GAG	NNP
cord-253024-b393ea2u	114	14	GCG	GCG	NNP
cord-253024-b393ea2u	114	15	CTT	CTT	NNP
cord-253024-b393ea2u	114	16	C	C	NNP
cord-253024-b393ea2u	114	17	)	)	-RRB-
cord-253024-b393ea2u	114	18	and	and	CC
cord-253024-b393ea2u	114	19	4055	4055	CD
cord-253024-b393ea2u	114	20	-	-	HYPH
cord-253024-b393ea2u	114	21	4070	4070	CD
cord-253024-b393ea2u	114	22	(	(	-LRB-
cord-253024-b393ea2u	114	23	GTC	GTC	NNP
cord-253024-b393ea2u	114	24	llT	llt	NN
cord-253024-b393ea2u	114	25	CCA	CCA	NNP
cord-253024-b393ea2u	114	26	GGA	GGA	NNP
cord-253024-b393ea2u	114	27	GAG	gag	NN
cord-253024-b393ea2u	114	28	G	G	NNP
cord-253024-b393ea2u	114	29	)	)	-RRB-
cord-253024-b393ea2u	114	30	and	and	CC
cord-253024-b393ea2u	114	31	were	be	VBD
cord-253024-b393ea2u	114	32	used	use	VBN
cord-253024-b393ea2u	114	33	to	to	TO
cord-253024-b393ea2u	114	34	amplify	amplify	VB
cord-253024-b393ea2u	114	35	a	a	DT
cord-253024-b393ea2u	114	36	-2.0-kb	-2.0-kb	CD
cord-253024-b393ea2u	114	37	fragment	fragment	NN
cord-253024-b393ea2u	114	38	spanning	span	VBG
cord-253024-b393ea2u	114	39	the	the	DT
cord-253024-b393ea2u	114	40	C	C	NNP
cord-253024-b393ea2u	114	41	terminus	terminus	NN
cord-253024-b393ea2u	114	42	of	of	IN
cord-253024-b393ea2u	114	43	the	the	DT
cord-253024-b393ea2u	114	44	S	S	NNP
cord-253024-b393ea2u	114	45	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	114	46	gene	gene	NN
cord-253024-b393ea2u	114	47	.	.	.
cord-253024-b393ea2u	115	1	Internal	internal	JJ
cord-253024-b393ea2u	115	2	oligodeoxynucleotides	oligodeoxynucleotide	NNS
cord-253024-b393ea2u	115	3	were	be	VBD
cord-253024-b393ea2u	115	4	used	use	VBN
cord-253024-b393ea2u	115	5	as	as	IN
cord-253024-b393ea2u	115	6	probe	probe	NN
cord-253024-b393ea2u	115	7	to	to	TO
cord-253024-b393ea2u	115	8	demonstrate	demonstrate	VB
cord-253024-b393ea2u	115	9	the	the	DT
cord-253024-b393ea2u	115	10	specificity	specificity	NN
cord-253024-b393ea2u	115	11	of	of	IN
cord-253024-b393ea2u	115	12	the	the	DT
cord-253024-b393ea2u	115	13	PCR	PCR	NNP
cord-253024-b393ea2u	115	14	reaction	reaction	NN
cord-253024-b393ea2u	115	15	.	.	.
cord-253024-b393ea2u	116	1	Six	six	CD
cord-253024-b393ea2u	116	2	complementation	complementation	NN
cord-253024-b393ea2u	116	3	group	group	NN
cord-253024-b393ea2u	116	4	F	F	NNP
cord-253024-b393ea2u	116	5	RNA+	RNA+	NNP
cord-253024-b393ea2u	116	6	mutants	mutant	NNS
cord-253024-b393ea2u	116	7	and	and	CC
cord-253024-b393ea2u	116	8	one	one	CD
cord-253024-b393ea2u	116	9	group	group	NN
cord-253024-b393ea2u	116	10	E	E	NNP
cord-253024-b393ea2u	116	11	RNA	rna	NN
cord-253024-b393ea2u	116	12	-	-	HYPH
cord-253024-b393ea2u	116	13	mutant	mutant	NN
cord-253024-b393ea2u	116	14	were	be	VBD
cord-253024-b393ea2u	116	15	used	use	VBN
cord-253024-b393ea2u	116	16	during	during	IN
cord-253024-b393ea2u	116	17	the	the	DT
cord-253024-b393ea2u	116	18	course	course	NN
cord-253024-b393ea2u	116	19	of	of	IN
cord-253024-b393ea2u	116	20	this	this	DT
cord-253024-b393ea2u	116	21	study	study	NN
cord-253024-b393ea2u	116	22	.	.	.
cord-253024-b393ea2u	117	1	Three	three	CD
cord-253024-b393ea2u	117	2	of	of	IN
cord-253024-b393ea2u	117	3	the	the	DT
cord-253024-b393ea2u	117	4	RNA	RNA	NNP
cord-253024-b393ea2u	117	5	'	'	POS
cord-253024-b393ea2u	117	6	mutants	mutant	NNS
cord-253024-b393ea2u	117	7	(	(	-LRB-
cord-253024-b393ea2u	117	8	LA7	LA7	NNP
cord-253024-b393ea2u	117	9	,	,	,
cord-253024-b393ea2u	117	10	LA1	LA1	NNP
cord-253024-b393ea2u	117	11	2	2	CD
cord-253024-b393ea2u	117	12	,	,	,
cord-253024-b393ea2u	117	13	 	 	_SP
cord-253024-b393ea2u	117	14	3.0	3.0	CD
cord-253024-b393ea2u	118	1	x	x	LS
cord-253024-b393ea2u	118	2	lo7	lo7	NNP
cord-253024-b393ea2u	118	3	1.0	1.0	CD
cord-253024-b393ea2u	118	4	x	x	SYM
cord-253024-b393ea2u	118	5	103	103	CD
cord-253024-b393ea2u	119	1	3.3	3.3	CD
cord-253024-b393ea2u	119	2	x	x	SYM
cord-253024-b393ea2u	119	3	1o-4	1o-4	CD
cord-253024-b393ea2u	120	1	1.1	1.1	CD
cord-253024-b393ea2u	120	2	x	x	SYM
cord-253024-b393ea2u	120	3	lo	lo	NNP
cord-253024-b393ea2u	120	4	*	*	NFP
cord-253024-b393ea2u	121	1	1.0	1.0	CD
cord-253024-b393ea2u	121	2	x	x	SYM
cord-253024-b393ea2u	121	3	lo2	lo2	NNP
cord-253024-b393ea2u	121	4	1.0	1.0	CD
cord-253024-b393ea2u	121	5	x	x	SYM
cord-253024-b393ea2u	121	6	10	10	CD
cord-253024-b393ea2u	121	7	-	-	SYM
cord-253024-b393ea2u	121	8	6	6	CD
cord-253024-b393ea2u	121	9	5.5	5.5	CD
cord-253024-b393ea2u	121	10	x	x	SYM
cord-253024-b393ea2u	121	11	107	107	CD
cord-253024-b393ea2u	122	1	1.3	1.3	CD
cord-253024-b393ea2u	122	2	x	x	SYM
cord-253024-b393ea2u	122	3	lo	lo	NNP
cord-253024-b393ea2u	122	4	*	*	NFP
cord-253024-b393ea2u	122	5	2.4	2.4	CD
cord-253024-b393ea2u	122	6	X	x	NN
cord-253024-b393ea2u	122	7	1O-6	1o-6	CD
cord-253024-b393ea2u	123	1	5.5	5.5	CD
cord-253024-b393ea2u	124	1	x	x	LS
cord-253024-b393ea2u	125	1	lo7	lo7	NNP
cord-253024-b393ea2u	125	2	9.5	9.5	CD
cord-253024-b393ea2u	125	3	x	x	SYM
cord-253024-b393ea2u	125	4	lo4	lo4	NNP
cord-253024-b393ea2u	125	5	1.7	1.7	CD
cord-253024-b393ea2u	125	6	x	x	SYM
cord-253024-b393ea2u	125	7	10	10	CD
cord-253024-b393ea2u	125	8	-	-	SYM
cord-253024-b393ea2u	125	9	3	3	CD
cord-253024-b393ea2u	125	10	3.5	3.5	CD
cord-253024-b393ea2u	125	11	x	x	SYM
cord-253024-b393ea2u	125	12	10	10	CD
cord-253024-b393ea2u	125	13	'	'	''
cord-253024-b393ea2u	126	1	4.3	4.3	CD
cord-253024-b393ea2u	126	2	x	x	SYM
cord-253024-b393ea2u	126	3	lo3	lo3	JJ
cord-253024-b393ea2u	126	4	1.2	1.2	CD
cord-253024-b393ea2u	126	5	x	x	SYM
cord-253024-b393ea2u	126	6	1o-4	1o-4	CD
cord-253024-b393ea2u	126	7	7.8	7.8	CD
cord-253024-b393ea2u	126	8	x	x	SYM
cord-253024-b393ea2u	126	9	10	10	CD
cord-253024-b393ea2u	126	10	'	'	POS
cord-253024-b393ea2u	126	11	1.4	1.4	CD
cord-253024-b393ea2u	126	12	x	x	SYM
cord-253024-b393ea2u	126	13	lo4	lo4	NNP
cord-253024-b393ea2u	126	14	1.8	1.8	CD
cord-253024-b393ea2u	126	15	x	x	SYM
cord-253024-b393ea2u	126	16	1O-4	1o-4	CD
cord-253024-b393ea2u	127	1	6.9	6.9	CD
cord-253024-b393ea2u	127	2	X	x	NN
cord-253024-b393ea2u	127	3	10	10	CD
cord-253024-b393ea2u	128	1	'	'	``
cord-253024-b393ea2u	128	2	8.7	8.7	CD
cord-253024-b393ea2u	128	3	x	x	SYM
cord-253024-b393ea2u	128	4	10	10	CD
cord-253024-b393ea2u	128	5	'	'	POS
cord-253024-b393ea2u	128	6	1.2	1.2	CD
cord-253024-b393ea2u	128	7	x	x	NN
cord-253024-b393ea2u	128	8	loo	loo	NN
cord-253024-b393ea2u	129	1	7.3	7.3	CD
cord-253024-b393ea2u	129	2	x	x	NN
cord-253024-b393ea2u	129	3	lo7	lo7	NN
cord-253024-b393ea2u	130	1	7.2	7.2	CD
cord-253024-b393ea2u	130	2	X	x	NN
cord-253024-b393ea2u	130	3	10	10	CD
cord-253024-b393ea2u	130	4	'	'	''
cord-253024-b393ea2u	131	1	1.0	1.0	CD
cord-253024-b393ea2u	131	2	x	x	SYM
cord-253024-b393ea2u	131	3	loo	loo	VBP
cord-253024-b393ea2u	131	4	9.1	9.1	CD
cord-253024-b393ea2u	131	5	x	x	SYM
cord-253024-b393ea2u	131	6	10	10	CD
cord-253024-b393ea2u	131	7	'	'	''
cord-253024-b393ea2u	132	1	9.5	9.5	CD
cord-253024-b393ea2u	132	2	x	x	NN
cord-253024-b393ea2u	132	3	lo7	lo7	NN
cord-253024-b393ea2u	132	4	1.0	1.0	CD
cord-253024-b393ea2u	132	5	x	x	SYM
cord-253024-b393ea2u	132	6	loo	loo	NN
cord-253024-b393ea2u	133	1	2.1	2.1	CD
cord-253024-b393ea2u	134	1	x	x	LS
cord-253024-b393ea2u	134	2	lo7	lo7	NNP
cord-253024-b393ea2u	135	1	2.0	2.0	CD
cord-253024-b393ea2u	135	2	x	x	SYM
cord-253024-b393ea2u	135	3	10	10	CD
cord-253024-b393ea2u	135	4	'	'	POS
cord-253024-b393ea2u	135	5	9.5	9.5	CD
cord-253024-b393ea2u	135	6	x	x	SYM
cord-253024-b393ea2u	135	7	10-l	10-l	CD
cord-253024-b393ea2u	135	8	8.5	8.5	CD
cord-253024-b393ea2u	135	9	X	x	NN
cord-253024-b393ea2u	135	10	lo	lo	NNP
cord-253024-b393ea2u	135	11	*	*	NFP
cord-253024-b393ea2u	135	12	5.5	5.5	CD
cord-253024-b393ea2u	135	13	x	x	SYM
cord-253024-b393ea2u	135	14	10	10	CD
cord-253024-b393ea2u	135	15	"	"	``
cord-253024-b393ea2u	135	16	6.4	6.4	CD
cord-253024-b393ea2u	135	17	X	x	NN
cord-253024-b393ea2u	135	18	lo-	lo-	JJ
cord-253024-b393ea2u	135	19	'	'	CC
cord-253024-b393ea2u	135	20	9.1	9.1	CD
cord-253024-b393ea2u	135	21	x	x	CD
cord-253024-b393ea2u	135	22	lo7	lo7	NN
cord-253024-b393ea2u	135	23	7.2	7.2	CD
cord-253024-b393ea2u	135	24	X	x	NN
cord-253024-b393ea2u	135	25	10	10	CD
cord-253024-b393ea2u	135	26	"	"	CD
cord-253024-b393ea2u	135	27	7.9	7.9	CD
cord-253024-b393ea2u	135	28	x	x	SYM
cord-253024-b393ea2u	135	29	10-Z	10-z	CD
cord-253024-b393ea2u	135	30	2.2	2.2	CD
cord-253024-b393ea2u	135	31	x	x	SYM
cord-253024-b393ea2u	135	32	10	10	CD
cord-253024-b393ea2u	135	33	'	'	CD
cord-253024-b393ea2u	135	34	2.4	2.4	CD
cord-253024-b393ea2u	135	35	X	x	NN
cord-253024-b393ea2u	135	36	lo7	lo7	NN
cord-253024-b393ea2u	136	1	1.1	1.1	CD
cord-253024-b393ea2u	136	2	x	x	SYM
cord-253024-b393ea2u	136	3	loo	loo	NN
cord-253024-b393ea2u	136	4	4.0	4.0	CD
cord-253024-b393ea2u	136	5	x	x	SYM
cord-253024-b393ea2u	136	6	10	10	CD
cord-253024-b393ea2u	137	1	'	'	``
cord-253024-b393ea2u	137	2	2.0	2.0	CD
cord-253024-b393ea2u	137	3	x	x	SYM
cord-253024-b393ea2u	137	4	lo6	lo6	VBN
cord-253024-b393ea2u	137	5	5.0	5.0	CD
cord-253024-b393ea2u	137	6	x	x	SYM
cord-253024-b393ea2u	137	7	10	10	CD
cord-253024-b393ea2u	137	8	-	-	SYM
cord-253024-b393ea2u	137	9	2	2	CD
cord-253024-b393ea2u	137	10	8.6	8.6	CD
cord-253024-b393ea2u	138	1	x	x	SYM
cord-253024-b393ea2u	138	2	10	10	CD
cord-253024-b393ea2u	138	3	'	'	''
cord-253024-b393ea2u	139	1	8.4	8.4	CD
cord-253024-b393ea2u	139	2	X	x	SYM
cord-253024-b393ea2u	139	3	10	10	CD
cord-253024-b393ea2u	139	4	'	'	''
cord-253024-b393ea2u	140	1	9.7	9.7	CD
cord-253024-b393ea2u	140	2	x	x	SYM
cord-253024-b393ea2u	140	3	10-l	10-l	CD
cord-253024-b393ea2u	140	4	1.2	1.2	CD
cord-253024-b393ea2u	140	5	x	x	NN
cord-253024-b393ea2u	140	6	lo8	lo8	NNP
cord-253024-b393ea2u	141	1	1.2	1.2	CD
cord-253024-b393ea2u	141	2	x	x	SYM
cord-253024-b393ea2u	141	3	lo8	lo8	NNP
cord-253024-b393ea2u	141	4	1.0	1.0	CD
cord-253024-b393ea2u	141	5	x	x	SYM
cord-253024-b393ea2u	141	6	lo0	lo0	NNP
cord-253024-b393ea2u	142	1	1.2	1.2	CD
cord-253024-b393ea2u	142	2	x	x	SYM
cord-253024-b393ea2u	142	3	lo8	lo8	NNP
cord-253024-b393ea2u	143	1	1.2	1.2	CD
cord-253024-b393ea2u	143	2	x	x	SYM
cord-253024-b393ea2u	143	3	108	108	CD
cord-253024-b393ea2u	143	4	1.0	1.0	CD
cord-253024-b393ea2u	143	5	x	x	SYM
cord-253024-b393ea2u	143	6	loo	loo	NN
cord-253024-b393ea2u	143	7	9.4	9.4	CD
cord-253024-b393ea2u	143	8	x	x	SYM
cord-253024-b393ea2u	143	9	10	10	CD
cord-253024-b393ea2u	143	10	'	'	''
cord-253024-b393ea2u	144	1	1.2	1.2	CD
cord-253024-b393ea2u	144	2	x	x	SYM
cord-253024-b393ea2u	144	3	108	108	CD
cord-253024-b393ea2u	144	4	1.2	1.2	CD
cord-253024-b393ea2u	144	5	x	x	NN
cord-253024-b393ea2u	144	6	loo	loo	NN
cord-253024-b393ea2u	144	7	8.2	8.2	CD
cord-253024-b393ea2u	144	8	X	x	NN
cord-253024-b393ea2u	144	9	lo7	lo7	NN
cord-253024-b393ea2u	144	10	9.2	9.2	CD
cord-253024-b393ea2u	144	11	x	x	NN
cord-253024-b393ea2u	144	12	lo7	lo7	NN
cord-253024-b393ea2u	144	13	1.1	1.1	CD
cord-253024-b393ea2u	144	14	x	x	SYM
cord-253024-b393ea2u	144	15	lo0	lo0	NNP
cord-253024-b393ea2u	144	16	1.0	1.0	CD
cord-253024-b393ea2u	144	17	x	x	SYM
cord-253024-b393ea2u	144	18	108	108	CD
cord-253024-b393ea2u	145	1	9.0	9.0	CD
cord-253024-b393ea2u	145	2	x	x	SYM
cord-253024-b393ea2u	145	3	lo7	lo7	NN
cord-253024-b393ea2u	145	4	9.0	9.0	CD
cord-253024-b393ea2u	146	1	x	x	LS
cord-253024-b393ea2u	146	2	10-l	10-l	CD
cord-253024-b393ea2u	146	3	9.2	9.2	CD
cord-253024-b393ea2u	146	4	x	x	SYM
cord-253024-b393ea2u	146	5	10	10	CD
cord-253024-b393ea2u	146	6	'	'	POS
cord-253024-b393ea2u	146	7	8.5	8.5	CD
cord-253024-b393ea2u	146	8	x	x	SYM
cord-253024-b393ea2u	146	9	10	10	CD
cord-253024-b393ea2u	146	10	'	'	''
cord-253024-b393ea2u	147	1	9.2	9.2	CD
cord-253024-b393ea2u	148	1	x	x	LS
cord-253024-b393ea2u	148	2	10-l	10-l	CD
cord-253024-b393ea2u	148	3	2.8	2.8	CD
cord-253024-b393ea2u	148	4	X	x	SYM
cord-253024-b393ea2u	148	5	10	10	CD
cord-253024-b393ea2u	148	6	'	'	''
cord-253024-b393ea2u	149	1	3.2	3.2	CD
cord-253024-b393ea2u	149	2	X	x	SYM
cord-253024-b393ea2u	149	3	10	10	CD
cord-253024-b393ea2u	149	4	'	'	POS
cord-253024-b393ea2u	149	5	1.1	1.1	CD
cord-253024-b393ea2u	149	6	x	x	SYM
cord-253024-b393ea2u	149	7	loo	loo	NN
cord-253024-b393ea2u	150	1	5.2	5.2	CD
cord-253024-b393ea2u	150	2	x	x	NFP
cord-253024-b393ea2u	151	1	lo7	lo7	NNP
cord-253024-b393ea2u	152	1	4.5	4.5	CD
cord-253024-b393ea2u	152	2	x	x	SYM
cord-253024-b393ea2u	152	3	10	10	CD
cord-253024-b393ea2u	152	4	'	'	CD
cord-253024-b393ea2u	152	5	8.6	8.6	CD
cord-253024-b393ea2u	152	6	x	x	SYM
cord-253024-b393ea2u	152	7	10-J	10-j	CD
cord-253024-b393ea2u	152	8	4.7	4.7	CD
cord-253024-b393ea2u	152	9	x	x	SYM
cord-253024-b393ea2u	152	10	10	10	CD
cord-253024-b393ea2u	152	11	'	'	''
cord-253024-b393ea2u	153	1	4.6	4.6	CD
cord-253024-b393ea2u	153	2	X	x	NN
cord-253024-b393ea2u	153	3	10	10	CD
cord-253024-b393ea2u	153	4	'	'	''
cord-253024-b393ea2u	154	1	9.7	9.7	CD
cord-253024-b393ea2u	154	2	x	x	SYM
cord-253024-b393ea2u	154	3	10-l	10-l	CD
cord-253024-b393ea2u	154	4	5.9	5.9	CD
cord-253024-b393ea2u	154	5	x	x	SYM
cord-253024-b393ea2u	154	6	10	10	CD
cord-253024-b393ea2u	154	7	'	'	''
cord-253024-b393ea2u	155	1	4.8	4.8	CD
cord-253024-b393ea2u	155	2	X	x	SYM
cord-253024-b393ea2u	155	3	10	10	CD
cord-253024-b393ea2u	155	4	'	'	''
cord-253024-b393ea2u	156	1	8.1	8.1	CD
cord-253024-b393ea2u	156	2	x	x	SYM
cord-253024-b393ea2u	156	3	10-l	10-l	CD
cord-253024-b393ea2u	156	4	+	+	CC
cord-253024-b393ea2u	156	5	+	+	CC
cord-253024-b393ea2u	157	1	+	+	CC
cord-253024-b393ea2u	158	1	+	+	CC
cord-253024-b393ea2u	159	1	+	+	CC
cord-253024-b393ea2u	160	1	+	+	CC
cord-253024-b393ea2u	161	1	+	+	CC
cord-253024-b393ea2u	161	2	+	+	CC
cord-253024-b393ea2u	161	3	"	"	``
cord-253024-b393ea2u	161	4	Calculated	calculate	VBN
cord-253024-b393ea2u	161	5	as	as	IN
cord-253024-b393ea2u	161	6	39.5132	39.5132	CD
cord-253024-b393ea2u	161	7	"	"	``
cord-253024-b393ea2u	161	8	NC6	NC6	NNP
cord-253024-b393ea2u	161	9	)	)	-RRB-
cord-253024-b393ea2u	161	10	and	and	CC
cord-253024-b393ea2u	161	11	one	one	CD
cord-253024-b393ea2u	161	12	RNA	rna	NN
cord-253024-b393ea2u	161	13	-	-	HYPH
cord-253024-b393ea2u	161	14	mutant	mutant	JJ
cord-253024-b393ea2u	161	15	(	(	-LRB-
cord-253024-b393ea2u	161	16	LA1	LA1	NNP
cord-253024-b393ea2u	161	17	8)	8)	NNP
cord-253024-b393ea2u	161	18	have	have	VBP
cord-253024-b393ea2u	161	19	been	be	VBN
cord-253024-b393ea2u	161	20	characterized	characterize	VBN
cord-253024-b393ea2u	161	21	previously	previously	RB
cord-253024-b393ea2u	162	1	(	(	-LRB-
cord-253024-b393ea2u	162	2	Schaad	Schaad	NNP
cord-253024-b393ea2u	162	3	el	el	NNP
cord-253024-b393ea2u	162	4	al	al	NNP
cord-253024-b393ea2u	162	5	.	.	NNP
cord-253024-b393ea2u	162	6	,	,	,
cord-253024-b393ea2u	162	7	1990	1990	CD
cord-253024-b393ea2u	162	8	)	)	-RRB-
cord-253024-b393ea2u	162	9	.	.	.
cord-253024-b393ea2u	163	1	To	to	TO
cord-253024-b393ea2u	163	2	increase	increase	VB
cord-253024-b393ea2u	163	3	the	the	DT
cord-253024-b393ea2u	163	4	accuracy	accuracy	NN
cord-253024-b393ea2u	163	5	of	of	IN
cord-253024-b393ea2u	163	6	the	the	DT
cord-253024-b393ea2u	163	7	recombination	recombination	NN
cord-253024-b393ea2u	163	8	map	map	NN
cord-253024-b393ea2u	163	9	and	and	CC
cord-253024-b393ea2u	163	10	obtain	obtain	VB
cord-253024-b393ea2u	163	11	more	more	JJR
cord-253024-b393ea2u	163	12	representative	representative	JJ
cord-253024-b393ea2u	163	13	mutants	mutant	NNS
cord-253024-b393ea2u	163	14	in	in	IN
cord-253024-b393ea2u	163	15	the	the	DT
cord-253024-b393ea2u	163	16	S	S	NNP
cord-253024-b393ea2u	163	17	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	163	18	gene	gene	NN
cord-253024-b393ea2u	163	19	,	,	,
cord-253024-b393ea2u	163	20	three	three	CD
cord-253024-b393ea2u	163	21	additional	additional	JJ
cord-253024-b393ea2u	163	22	RNA+	RNA+	NNP
cord-253024-b393ea2u	163	23	mutants	mutant	NNS
cord-253024-b393ea2u	163	24	were	be	VBD
cord-253024-b393ea2u	163	25	isolated	isolate	VBN
cord-253024-b393ea2u	163	26	by	by	IN
cord-253024-b393ea2u	163	27	mutagenesis	mutagenesis	NN
cord-253024-b393ea2u	163	28	as	as	IN
cord-253024-b393ea2u	163	29	previously	previously	RB
cord-253024-b393ea2u	163	30	described	describe	VBN
cord-253024-b393ea2u	163	31	.	.	.
cord-253024-b393ea2u	164	1	Two	two	CD
cord-253024-b393ea2u	164	2	mutants	mutant	NNS
cord-253024-b393ea2u	164	3	were	be	VBD
cord-253024-b393ea2u	164	4	isolated	isolate	VBN
cord-253024-b393ea2u	164	5	following	follow	VBG
cord-253024-b393ea2u	164	6	mutagenesis	mutagenesis	NN
cord-253024-b393ea2u	164	7	with	with	IN
cord-253024-b393ea2u	164	8	100	100	CD
cord-253024-b393ea2u	164	9	pg	pg	NN
cord-253024-b393ea2u	164	10	/	/	SYM
cord-253024-b393ea2u	164	11	ml	ml	NN
cord-253024-b393ea2u	164	12	5-fluorouracil	5-fluorouracil	CD
cord-253024-b393ea2u	164	13	(	(	-LRB-
cord-253024-b393ea2u	164	14	NC14	NC14	NNP
cord-253024-b393ea2u	164	15	,	,	,
cord-253024-b393ea2u	164	16	NC16	NC16	NNP
cord-253024-b393ea2u	164	17	)	)	-RRB-
cord-253024-b393ea2u	164	18	and	and	CC
cord-253024-b393ea2u	164	19	one	one	CD
cord-253024-b393ea2u	164	20	mutant	mutant	JJ
cord-253024-b393ea2u	164	21	with	with	IN
cord-253024-b393ea2u	164	22	20	20	CD
cord-253024-b393ea2u	164	23	pg	pg	NN
cord-253024-b393ea2u	164	24	/	/	SYM
cord-253024-b393ea2u	164	25	ml	ml	NN
cord-253024-b393ea2u	164	26	5-azacytidine	5-azacytidine	CD
cord-253024-b393ea2u	164	27	(	(	-LRB-
cord-253024-b393ea2u	164	28	NC17	NC17	NNP
cord-253024-b393ea2u	164	29	)	)	-RRB-
cord-253024-b393ea2u	164	30	.	.	.
cord-253024-b393ea2u	165	1	All	all	DT
cord-253024-b393ea2u	165	2	mutants	mutant	NNS
cord-253024-b393ea2u	165	3	had	have	VBD
cord-253024-b393ea2u	165	4	reversion	reversion	NN
cord-253024-b393ea2u	165	5	frequencies	frequency	NNS
cord-253024-b393ea2u	165	6	of	of	IN
cord-253024-b393ea2u	165	7	less	less	JJR
cord-253024-b393ea2u	165	8	than	than	IN
cord-253024-b393ea2u	165	9	10e3	10e3	CD
cord-253024-b393ea2u	165	10	,	,	,
cord-253024-b393ea2u	165	11	and	and	CC
cord-253024-b393ea2u	165	12	did	do	VBD
cord-253024-b393ea2u	165	13	not	not	RB
cord-253024-b393ea2u	165	14	complement	complement	VB
cord-253024-b393ea2u	165	15	each	each	DT
cord-253024-b393ea2u	165	16	others	other	NNS
cord-253024-b393ea2u	165	17	defect	defect	VBP
cord-253024-b393ea2u	165	18	at	at	IN
cord-253024-b393ea2u	165	19	the	the	DT
cord-253024-b393ea2u	165	20	restrictive	restrictive	JJ
cord-253024-b393ea2u	165	21	temperature	temperature	NN
cord-253024-b393ea2u	165	22	(	(	-LRB-
cord-253024-b393ea2u	165	23	Table	table	NN
cord-253024-b393ea2u	165	24	1	1	CD
cord-253024-b393ea2u	165	25	,	,	,
cord-253024-b393ea2u	165	26	data	datum	NNS
cord-253024-b393ea2u	165	27	not	not	RB
cord-253024-b393ea2u	165	28	shown	show	VBN
cord-253024-b393ea2u	165	29	)	)	-RRB-
cord-253024-b393ea2u	165	30	.	.	.
cord-253024-b393ea2u	166	1	All	all	DT
cord-253024-b393ea2u	166	2	of	of	IN
cord-253024-b393ea2u	166	3	the	the	DT
cord-253024-b393ea2u	166	4	RNA+	RNA+	NNP
cord-253024-b393ea2u	166	5	mutants	mutant	NNS
cord-253024-b393ea2u	166	6	were	be	VBD
cord-253024-b393ea2u	166	7	capable	capable	JJ
cord-253024-b393ea2u	166	8	of	of	IN
cord-253024-b393ea2u	166	9	transcribing	transcribe	VBG
cord-253024-b393ea2u	166	10	viral	viral	JJ
cord-253024-b393ea2u	166	11	mRNA	mrna	NN
cord-253024-b393ea2u	166	12	when	when	WRB
cord-253024-b393ea2u	166	13	maintained	maintain	VBN
cord-253024-b393ea2u	166	14	at	at	IN
cord-253024-b393ea2u	166	15	the	the	DT
cord-253024-b393ea2u	166	16	restrictive	restrictive	JJ
cord-253024-b393ea2u	166	17	temperature	temperature	NN
cord-253024-b393ea2u	166	18	(	(	-LRB-
cord-253024-b393ea2u	166	19	data	datum	NNS
cord-253024-b393ea2u	166	20	not	not	RB
cord-253024-b393ea2u	166	21	shown	show	VBN
cord-253024-b393ea2u	166	22	,	,	,
cord-253024-b393ea2u	166	23	Table	Table	NNP
cord-253024-b393ea2u	166	24	1	1	CD
cord-253024-b393ea2u	166	25	)	)	-RRB-
cord-253024-b393ea2u	166	26	.	.	.
cord-253024-b393ea2u	167	1	While	while	IN
cord-253024-b393ea2u	167	2	NC14	NC14	NNP
cord-253024-b393ea2u	167	3	produced	produce	VBD
cord-253024-b393ea2u	167	4	a	a	DT
cord-253024-b393ea2u	167	5	small	small	JJ
cord-253024-b393ea2u	167	6	amount	amount	NN
cord-253024-b393ea2u	167	7	syncytium	syncytium	NN
cord-253024-b393ea2u	167	8	,	,	,
cord-253024-b393ea2u	167	9	the	the	DT
cord-253024-b393ea2u	167	10	remainder	remainder	NN
cord-253024-b393ea2u	167	11	of	of	IN
cord-253024-b393ea2u	167	12	the	the	DT
cord-253024-b393ea2u	167	13	mutants	mutant	NNS
cord-253024-b393ea2u	167	14	were	be	VBD
cord-253024-b393ea2u	167	15	not	not	RB
cord-253024-b393ea2u	167	16	capable	capable	JJ
cord-253024-b393ea2u	167	17	of	of	IN
cord-253024-b393ea2u	167	18	giant	giant	JJ
cord-253024-b393ea2u	167	19	cell	cell	NN
cord-253024-b393ea2u	167	20	formation	formation	NN
cord-253024-b393ea2u	167	21	at	at	IN
cord-253024-b393ea2u	167	22	the	the	DT
cord-253024-b393ea2u	167	23	restrictive	restrictive	JJ
cord-253024-b393ea2u	167	24	temperature	temperature	NN
cord-253024-b393ea2u	167	25	(	(	-LRB-
cord-253024-b393ea2u	167	26	Fig	fig	NN
cord-253024-b393ea2u	167	27	.	.	.
cord-253024-b393ea2u	167	28	1	1	CD
cord-253024-b393ea2u	167	29	)	)	-RRB-
cord-253024-b393ea2u	167	30	.	.	.
cord-253024-b393ea2u	168	1	To	to	TO
cord-253024-b393ea2u	168	2	assist	assist	VB
cord-253024-b393ea2u	168	3	in	in	IN
cord-253024-b393ea2u	168	4	precisely	precisely	RB
cord-253024-b393ea2u	168	5	defining	define	VBG
cord-253024-b393ea2u	168	6	the	the	DT
cord-253024-b393ea2u	168	7	location	location	NN
cord-253024-b393ea2u	168	8	of	of	IN
cord-253024-b393ea2u	168	9	these	these	DT
cord-253024-b393ea2u	168	10	mutations	mutation	NNS
cord-253024-b393ea2u	168	11	and	and	CC
cord-253024-b393ea2u	168	12	examining	examine	VBG
cord-253024-b393ea2u	168	13	the	the	DT
cord-253024-b393ea2u	168	14	mechanism	mechanism	NN
cord-253024-b393ea2u	168	15	for	for	IN
cord-253024-b393ea2u	168	16	MHV	MHV	NNP
cord-253024-b393ea2u	168	17	RNA	RNA	NNP
cord-253024-b393ea2u	168	18	recombination	recombination	NN
cord-253024-b393ea2u	168	19	within	within	IN
cord-253024-b393ea2u	168	20	the	the	DT
cord-253024-b393ea2u	168	21	S	S	NNP
cord-253024-b393ea2u	168	22	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	168	23	gene	gene	NN
cord-253024-b393ea2u	168	24	,	,	,
cord-253024-b393ea2u	168	25	a	a	DT
cord-253024-b393ea2u	168	26	panel	panel	NN
cord-253024-b393ea2u	168	27	of	of	IN
cord-253024-b393ea2u	168	28	revertants	revertant	NNS
cord-253024-b393ea2u	168	29	were	be	VBD
cord-253024-b393ea2u	168	30	obtained	obtain	VBN
cord-253024-b393ea2u	168	31	from	from	IN
cord-253024-b393ea2u	168	32	six	six	CD
cord-253024-b393ea2u	168	33	of	of	IN
cord-253024-b393ea2u	168	34	the	the	DT
cord-253024-b393ea2u	168	35	RNA+	RNA+	NNP
cord-253024-b393ea2u	168	36	mutants	mutant	NNS
cord-253024-b393ea2u	168	37	(	(	-LRB-
cord-253024-b393ea2u	168	38	Table	table	NN
cord-253024-b393ea2u	168	39	1	1	CD
cord-253024-b393ea2u	168	40	)	)	-RRB-
cord-253024-b393ea2u	168	41	.	.	.
cord-253024-b393ea2u	169	1	Revertant	Revertant	NNP
cord-253024-b393ea2u	169	2	viruses	virus	NNS
cord-253024-b393ea2u	169	3	had	have	VBD
cord-253024-b393ea2u	169	4	similar	similar	JJ
cord-253024-b393ea2u	169	5	titers	titer	NNS
cord-253024-b393ea2u	169	6	,	,	,
cord-253024-b393ea2u	169	7	produced	produce	VBD
cord-253024-b393ea2u	169	8	syncytium	syncytium	NN
cord-253024-b393ea2u	169	9	,	,	,
cord-253024-b393ea2u	169	10	and	and	CC
cord-253024-b393ea2u	169	11	were	be	VBD
cord-253024-b393ea2u	169	12	of	of	IN
cord-253024-b393ea2u	169	13	the	the	DT
cord-253024-b393ea2u	169	14	RNA+	RNA+	NNP
cord-253024-b393ea2u	169	15	phenotype	phenotype	NN
cord-253024-b393ea2u	169	16	when	when	WRB
cord-253024-b393ea2u	169	17	assayed	assay	VBN
cord-253024-b393ea2u	169	18	at	at	IN
cord-253024-b393ea2u	169	19	both	both	CC
cord-253024-b393ea2u	169	20	permissive	permissive	JJ
cord-253024-b393ea2u	169	21	and	and	CC
cord-253024-b393ea2u	169	22	restrictive	restrictive	JJ
cord-253024-b393ea2u	169	23	temperatures	temperature	NNS
cord-253024-b393ea2u	169	24	.	.	.
cord-253024-b393ea2u	170	1	To	to	TO
cord-253024-b393ea2u	170	2	conclusively	conclusively	RB
cord-253024-b393ea2u	170	3	document	document	VB
cord-253024-b393ea2u	170	4	the	the	DT
cord-253024-b393ea2u	170	5	revertant	revertant	JJ
cord-253024-b393ea2u	170	6	phenotype	phenotype	NN
cord-253024-b393ea2u	170	7	and	and	CC
cord-253024-b393ea2u	170	8	determine	determine	VB
cord-253024-b393ea2u	170	9	if	if	IN
cord-253024-b393ea2u	170	10	growth	growth	NN
cord-253024-b393ea2u	170	11	characteristics	characteristic	NNS
cord-253024-b393ea2u	170	12	were	be	VBD
cord-253024-b393ea2u	170	13	similar	similar	JJ
cord-253024-b393ea2u	170	14	to	to	IN
cord-253024-b393ea2u	170	15	parental	parental	JJ
cord-253024-b393ea2u	170	16	controls	control	NNS
cord-253024-b393ea2u	170	17	,	,	,
cord-253024-b393ea2u	170	18	growth	growth	NN
cord-253024-b393ea2u	170	19	curves	curve	NNS
cord-253024-b393ea2u	170	20	were	be	VBD
cord-253024-b393ea2u	170	21	compared	compare	VBN
cord-253024-b393ea2u	170	22	at	at	IN
cord-253024-b393ea2u	170	23	the	the	DT
cord-253024-b393ea2u	170	24	permissive	permissive	JJ
cord-253024-b393ea2u	170	25	and	and	CC
cord-253024-b393ea2u	170	26	restrictive	restrictive	JJ
cord-253024-b393ea2u	170	27	temperatures	temperature	NNS
cord-253024-b393ea2u	170	28	.	.	.
cord-253024-b393ea2u	171	1	Cultures	culture	NNS
cord-253024-b393ea2u	171	2	of	of	IN
cord-253024-b393ea2u	171	3	cells	cell	NNS
cord-253024-b393ea2u	171	4	were	be	VBD
cord-253024-b393ea2u	171	5	infected	infect	VBN
cord-253024-b393ea2u	171	6	with	with	IN
cord-253024-b393ea2u	171	7	NC1	NC1	NNP
cord-253024-b393ea2u	171	8	6	6	CD
cord-253024-b393ea2u	171	9	or	or	CC
cord-253024-b393ea2u	171	10	its	-PRON-	PRP$
cord-253024-b393ea2u	171	11	revertant	revertant	NN
cord-253024-b393ea2u	171	12	,	,	,
cord-253024-b393ea2u	171	13	NC1	NC1	NNP
cord-253024-b393ea2u	171	14	6-Rl	6-Rl	NNP
cord-253024-b393ea2u	171	15	,	,	,
cord-253024-b393ea2u	171	16	at	at	IN
cord-253024-b393ea2u	171	17	32	32	CD
cord-253024-b393ea2u	171	18	"	"	''
cord-253024-b393ea2u	171	19	and	and	CC
cord-253024-b393ea2u	171	20	shifted	shift	VBD
cord-253024-b393ea2u	171	21	to	to	IN
cord-253024-b393ea2u	171	22	39.5	39.5	CD
cord-253024-b393ea2u	171	23	"	"	''
cord-253024-b393ea2u	171	24	at	at	IN
cord-253024-b393ea2u	171	25	5.0	5.0	CD
cord-253024-b393ea2u	171	26	hr	hr	NN
cord-253024-b393ea2u	171	27	postinfection	postinfection	NN
cord-253024-b393ea2u	171	28	.	.	.
cord-253024-b393ea2u	172	1	Viral	viral	JJ
cord-253024-b393ea2u	172	2	progeny	progeny	NNS
cord-253024-b393ea2u	172	3	were	be	VBD
cord-253024-b393ea2u	172	4	harvested	harvest	VBN
cord-253024-b393ea2u	172	5	at	at	IN
cord-253024-b393ea2u	172	6	different	different	JJ
cord-253024-b393ea2u	172	7	times	time	NNS
cord-253024-b393ea2u	172	8	after	after	IN
cord-253024-b393ea2u	172	9	infection	infection	NN
cord-253024-b393ea2u	172	10	and	and	CC
cord-253024-b393ea2u	172	11	assayed	assay	VBN
cord-253024-b393ea2u	172	12	by	by	IN
cord-253024-b393ea2u	172	13	plaque	plaque	NN
cord-253024-b393ea2u	172	14	assay	assay	NN
cord-253024-b393ea2u	172	15	at	at	IN
cord-253024-b393ea2u	172	16	the	the	DT
cord-253024-b393ea2u	172	17	permissive	permissive	JJ
cord-253024-b393ea2u	172	18	temperature	temperature	NN
cord-253024-b393ea2u	172	19	.	.	.
cord-253024-b393ea2u	173	1	Similar	similar	JJ
cord-253024-b393ea2u	173	2	growth	growth	NN
cord-253024-b393ea2u	173	3	curves	curve	NNS
cord-253024-b393ea2u	173	4	were	be	VBD
cord-253024-b393ea2u	173	5	evident	evident	JJ
cord-253024-b393ea2u	173	6	between	between	IN
cord-253024-b393ea2u	173	7	ts	ts	NNP
cord-253024-b393ea2u	173	8	NC1	NC1	NNP
cord-253024-b393ea2u	173	9	6	6	CD
cord-253024-b393ea2u	173	10	and	and	CC
cord-253024-b393ea2u	173	11	its	-PRON-	PRP$
cord-253024-b393ea2u	173	12	revertant	revertant	NN
cord-253024-b393ea2u	173	13	NC1	NC1	NNP
cord-253024-b393ea2u	173	14	6-Rl	6-rl	CD
cord-253024-b393ea2u	173	15	in	in	IN
cord-253024-b393ea2u	173	16	cultures	culture	NNS
cord-253024-b393ea2u	173	17	maintained	maintain	VBN
cord-253024-b393ea2u	173	18	at	at	IN
cord-253024-b393ea2u	173	19	the	the	DT
cord-253024-b393ea2u	173	20	permissive	permissive	JJ
cord-253024-b393ea2u	173	21	temperature	temperature	NN
cord-253024-b393ea2u	173	22	(	(	-LRB-
cord-253024-b393ea2u	173	23	Fig	fig	NN
cord-253024-b393ea2u	173	24	.	.	NNP
cord-253024-b393ea2u	173	25	2	2	CD
cord-253024-b393ea2u	173	26	)	)	-RRB-
cord-253024-b393ea2u	173	27	.	.	.
cord-253024-b393ea2u	174	1	However	however	RB
cord-253024-b393ea2u	174	2	,	,	,
cord-253024-b393ea2u	174	3	following	follow	VBG
cord-253024-b393ea2u	174	4	temperature	temperature	NN
cord-253024-b393ea2u	174	5	shift	shift	NN
cord-253024-b393ea2u	174	6	,	,	,
cord-253024-b393ea2u	174	7	growth	growth	NN
cord-253024-b393ea2u	174	8	of	of	IN
cord-253024-b393ea2u	174	9	ts	ts	NNP
cord-253024-b393ea2u	174	10	NC16	NC16	NNP
cord-253024-b393ea2u	174	11	was	be	VBD
cord-253024-b393ea2u	174	12	inhibited	inhibit	VBN
cord-253024-b393ea2u	174	13	significantly	significantly	RB
cord-253024-b393ea2u	174	14	under	under	IN
cord-253024-b393ea2u	174	15	conditions	condition	NNS
cord-253024-b393ea2u	174	16	in	in	IN
cord-253024-b393ea2u	174	17	which	which	WDT
cord-253024-b393ea2u	174	18	the	the	DT
cord-253024-b393ea2u	174	19	revertant	revertant	NN
cord-253024-b393ea2u	174	20	replicated	replicate	VBN
cord-253024-b393ea2u	174	21	normally	normally	RB
cord-253024-b393ea2u	174	22	.	.	.
cord-253024-b393ea2u	175	1	Similar	similar	JJ
cord-253024-b393ea2u	175	2	results	result	NNS
cord-253024-b393ea2u	175	3	have	have	VBP
cord-253024-b393ea2u	175	4	also	also	RB
cord-253024-b393ea2u	175	5	been	be	VBN
cord-253024-b393ea2u	175	6	obtained	obtain	VBN
cord-253024-b393ea2u	175	7	with	with	IN
cord-253024-b393ea2u	175	8	other	other	JJ
cord-253024-b393ea2u	175	9	mutants	mutant	NNS
cord-253024-b393ea2u	175	10	and	and	CC
cord-253024-b393ea2u	175	11	their	-PRON-	PRP$
cord-253024-b393ea2u	175	12	revertants	revertant	NNS
cord-253024-b393ea2u	175	13	(	(	-LRB-
cord-253024-b393ea2u	175	14	data	datum	NNS
cord-253024-b393ea2u	175	15	not	not	RB
cord-253024-b393ea2u	175	16	shown	show	VBN
cord-253024-b393ea2u	175	17	)	)	-RRB-
cord-253024-b393ea2u	175	18	.	.	.
cord-253024-b393ea2u	176	1	To	to	TO
cord-253024-b393ea2u	176	2	assess	assess	VB
cord-253024-b393ea2u	176	3	the	the	DT
cord-253024-b393ea2u	176	4	ability	ability	NN
cord-253024-b393ea2u	176	5	of	of	IN
cord-253024-b393ea2u	176	6	these	these	DT
cord-253024-b393ea2u	176	7	mutants	mutant	NNS
cord-253024-b393ea2u	176	8	to	to	TO
cord-253024-b393ea2u	176	9	be	be	VB
cord-253024-b393ea2u	176	10	used	use	VBN
cord-253024-b393ea2u	176	11	in	in	IN
cord-253024-b393ea2u	176	12	recombination	recombination	NN
cord-253024-b393ea2u	176	13	studies	study	NNS
cord-253024-b393ea2u	176	14	,	,	,
cord-253024-b393ea2u	176	15	we	-PRON-	PRP
cord-253024-b393ea2u	176	16	performed	perform	VBD
cord-253024-b393ea2u	176	17	a	a	DT
cord-253024-b393ea2u	176	18	series	series	NN
cord-253024-b393ea2u	176	19	of	of	IN
cord-253024-b393ea2u	176	20	crosses	crosse	NNS
cord-253024-b393ea2u	176	21	between	between	IN
cord-253024-b393ea2u	176	22	different	different	JJ
cord-253024-b393ea2u	176	23	group	group	NN
cord-253024-b393ea2u	176	24	F	f	NN
cord-253024-b393ea2u	176	25	ts	ts	NNP
cord-253024-b393ea2u	176	26	mutants	mutant	NNS
cord-253024-b393ea2u	176	27	and	and	CC
cord-253024-b393ea2u	176	28	examined	examine	VBD
cord-253024-b393ea2u	176	29	the	the	DT
cord-253024-b393ea2u	176	30	differences	difference	NNS
cord-253024-b393ea2u	176	31	between	between	IN
cord-253024-b393ea2u	176	32	the	the	DT
cord-253024-b393ea2u	176	33	recombination	recombination	NN
cord-253024-b393ea2u	176	34	and	and	CC
cord-253024-b393ea2u	176	35	reversion	reversion	NN
cord-253024-b393ea2u	176	36	frequencies	frequency	NNS
cord-253024-b393ea2u	176	37	.	.	.
cord-253024-b393ea2u	177	1	Cultures	culture	NNS
cord-253024-b393ea2u	177	2	of	of	IN
cord-253024-b393ea2u	177	3	cells	cell	NNS
cord-253024-b393ea2u	177	4	were	be	VBD
cord-253024-b393ea2u	177	5	infected	infect	VBN
cord-253024-b393ea2u	177	6	at	at	IN
cord-253024-b393ea2u	177	7	a	a	DT
cord-253024-b393ea2u	177	8	m.o.i	m.o.i	NN
cord-253024-b393ea2u	177	9	.	.	NN
cord-253024-b393ea2u	177	10	of	of	IN
cord-253024-b393ea2u	177	11	10	10	CD
cord-253024-b393ea2u	177	12	each	each	DT
cord-253024-b393ea2u	177	13	with	with	IN
cord-253024-b393ea2u	177	14	two	two	CD
cord-253024-b393ea2u	177	15	different	different	JJ
cord-253024-b393ea2u	177	16	ts	ts	NN
cord-253024-b393ea2u	177	17	mutants	mutant	NNS
cord-253024-b393ea2u	177	18	and	and	CC
cord-253024-b393ea2u	177	19	maintained	maintain	VBD
cord-253024-b393ea2u	177	20	at	at	IN
cord-253024-b393ea2u	177	21	32	32	CD
cord-253024-b393ea2u	177	22	"	"	``
cord-253024-b393ea2u	177	23	for	for	IN
cord-253024-b393ea2u	177	24	14	14	CD
cord-253024-b393ea2u	177	25	hr	hr	NN
cord-253024-b393ea2u	177	26	postinfection	postinfection	NN
cord-253024-b393ea2u	177	27	.	.	.
cord-253024-b393ea2u	178	1	The	the	DT
cord-253024-b393ea2u	178	2	progeny	progeny	NN
cord-253024-b393ea2u	178	3	were	be	VBD
cord-253024-b393ea2u	178	4	harvested	harvest	VBN
cord-253024-b393ea2u	178	5	and	and	CC
cord-253024-b393ea2u	178	6	titered	titere	VBN
cord-253024-b393ea2u	178	7	at	at	IN
cord-253024-b393ea2u	178	8	the	the	DT
cord-253024-b393ea2u	178	9	permissive	permissive	JJ
cord-253024-b393ea2u	178	10	and	and	CC
cord-253024-b393ea2u	178	11	restrictive	restrictive	JJ
cord-253024-b393ea2u	178	12	temperatures	temperature	NNS
cord-253024-b393ea2u	178	13	.	.	.
cord-253024-b393ea2u	179	1	Recombination	recombination	NN
cord-253024-b393ea2u	179	2	frequencies	frequency	NNS
cord-253024-b393ea2u	179	3	ranged	range	VBD
cord-253024-b393ea2u	179	4	from	from	IN
cord-253024-b393ea2u	179	5	as	as	RB
cord-253024-b393ea2u	179	6	little	little	JJ
cord-253024-b393ea2u	179	7	as	as	IN
cord-253024-b393ea2u	179	8	0.1	0.1	CD
cord-253024-b393ea2u	179	9	%	%	NN
cord-253024-b393ea2u	179	10	to	to	IN
cord-253024-b393ea2u	179	11	>	>	XX
cord-253024-b393ea2u	179	12	4.00/0	4.00/0	CD
cord-253024-b393ea2u	179	13	suggesting	suggest	VBG
cord-253024-b393ea2u	179	14	that	that	IN
cord-253024-b393ea2u	179	15	the	the	DT
cord-253024-b393ea2u	179	16	mutants	mutant	NNS
cord-253024-b393ea2u	179	17	map	map	VBP
cord-253024-b393ea2u	179	18	in	in	IN
cord-253024-b393ea2u	179	19	different	different	JJ
cord-253024-b393ea2u	179	20	locations	location	NNS
cord-253024-b393ea2u	179	21	in	in	IN
cord-253024-b393ea2u	179	22	the	the	DT
cord-253024-b393ea2u	179	23	gene	gene	NN
cord-253024-b393ea2u	179	24	.	.	.
cord-253024-b393ea2u	180	1	The	the	DT
cord-253024-b393ea2u	180	2	ratio	ratio	NN
cord-253024-b393ea2u	180	3	of	of	IN
cord-253024-b393ea2u	180	4	recombinants	recombinant	NNS
cord-253024-b393ea2u	180	5	to	to	IN
cord-253024-b393ea2u	180	6	revertants	revertant	NNS
cord-253024-b393ea2u	180	7	ranged	range	VBN
cord-253024-b393ea2u	180	8	from	from	IN
cord-253024-b393ea2u	180	9	9	9	CD
cord-253024-b393ea2u	180	10	to	to	IN
cord-253024-b393ea2u	180	11	3	3	CD
cord-253024-b393ea2u	180	12	140	140	CD
cord-253024-b393ea2u	180	13	times	time	NNS
cord-253024-b393ea2u	180	14	higher	high	JJR
cord-253024-b393ea2u	180	15	than	than	IN
cord-253024-b393ea2u	180	16	the	the	DT
cord-253024-b393ea2u	180	17	sum	sum	NN
cord-253024-b393ea2u	180	18	of	of	IN
cord-253024-b393ea2u	180	19	the	the	DT
cord-253024-b393ea2u	180	20	spontaneous	spontaneous	JJ
cord-253024-b393ea2u	180	21	reversion	reversion	NN
cord-253024-b393ea2u	180	22	frequencies	frequency	NNS
cord-253024-b393ea2u	180	23	of	of	IN
cord-253024-b393ea2u	180	24	each	each	DT
cord-253024-b393ea2u	180	25	individual	individual	JJ
cord-253024-b393ea2u	180	26	ts	ts	NN
cord-253024-b393ea2u	180	27	mutant	mutant	NN
cord-253024-b393ea2u	180	28	used	use	VBN
cord-253024-b393ea2u	180	29	in	in	IN
cord-253024-b393ea2u	180	30	the	the	DT
cord-253024-b393ea2u	180	31	cross	cross	NN
cord-253024-b393ea2u	180	32	and	and	CC
cord-253024-b393ea2u	180	33	indicated	indicate	VBD
cord-253024-b393ea2u	180	34	that	that	IN
cord-253024-b393ea2u	180	35	recombination	recombination	NN
cord-253024-b393ea2u	180	36	mapping	mapping	NN
cord-253024-b393ea2u	180	37	was	be	VBD
cord-253024-b393ea2u	180	38	feasible	feasible	JJ
cord-253024-b393ea2u	180	39	throughout	throughout	IN
cord-253024-b393ea2u	180	40	this	this	DT
cord-253024-b393ea2u	180	41	region	region	NN
cord-253024-b393ea2u	180	42	(	(	-LRB-
cord-253024-b393ea2u	180	43	Table	table	NN
cord-253024-b393ea2u	180	44	2	2	CD
cord-253024-b393ea2u	180	45	)	)	-RRB-
cord-253024-b393ea2u	180	46	.	.	.
cord-253024-b393ea2u	181	1	The	the	DT
cord-253024-b393ea2u	181	2	extent	extent	NN
cord-253024-b393ea2u	181	3	of	of	IN
cord-253024-b393ea2u	181	4	the	the	DT
cord-253024-b393ea2u	181	5	difference	difference	NN
cord-253024-b393ea2u	181	6	in	in	IN
cord-253024-b393ea2u	181	7	the	the	DT
cord-253024-b393ea2u	181	8	ratio	ratio	NN
cord-253024-b393ea2u	181	9	of	of	IN
cord-253024-b393ea2u	181	10	recombinants	recombinant	NNS
cord-253024-b393ea2u	181	11	to	to	IN
cord-253024-b393ea2u	181	12	revertants	revertant	NNS
cord-253024-b393ea2u	181	13	probably	probably	RB
cord-253024-b393ea2u	181	14	reflected	reflect	VBD
cord-253024-b393ea2u	181	15	the	the	DT
cord-253024-b393ea2u	181	16	stability	stability	NN
cord-253024-b393ea2u	181	17	of	of	IN
cord-253024-b393ea2u	181	18	the	the	DT
cord-253024-b393ea2u	181	19	mutants	mutant	NNS
cord-253024-b393ea2u	181	20	used	use	VBN
cord-253024-b393ea2u	181	21	in	in	IN
cord-253024-b393ea2u	181	22	the	the	DT
cord-253024-b393ea2u	181	23	cross	cross	NN
cord-253024-b393ea2u	181	24	and	and	CC
cord-253024-b393ea2u	181	25	the	the	DT
cord-253024-b393ea2u	181	26	distance	distance	NN
cord-253024-b393ea2u	181	27	between	between	IN
cord-253024-b393ea2u	181	28	the	the	DT
cord-253024-b393ea2u	181	29	individual	individual	JJ
cord-253024-b393ea2u	181	30	ts	ts	NN
cord-253024-b393ea2u	181	31	lesions	lesion	NNS
cord-253024-b393ea2u	181	32	.	.	.
cord-253024-b393ea2u	182	1	Establishing	establish	VBG
cord-253024-b393ea2u	182	2	a	a	DT
cord-253024-b393ea2u	182	3	recombination	recombination	NN
cord-253024-b393ea2u	182	4	map	map	NN
cord-253024-b393ea2u	182	5	for	for	IN
cord-253024-b393ea2u	182	6	the	the	DT
cord-253024-b393ea2u	182	7	group	group	NN
cord-253024-b393ea2u	182	8	F	F	NNP
cord-253024-b393ea2u	182	9	RNA+	RNA+	NNP
cord-253024-b393ea2u	182	10	mutants	mutant	NNS
cord-253024-b393ea2u	183	1	In	in	IN
cord-253024-b393ea2u	183	2	contrast	contrast	NN
cord-253024-b393ea2u	183	3	to	to	IN
cord-253024-b393ea2u	183	4	poliovirus	poliovirus	NN
cord-253024-b393ea2u	183	5	and	and	CC
cord-253024-b393ea2u	183	6	apthovirus	apthovirus	NNP
cord-253024-b393ea2u	183	7	ts	ts	NNP
cord-253024-b393ea2u	183	8	mutants	mutant	NNS
cord-253024-b393ea2u	183	9	,	,	,
cord-253024-b393ea2u	183	10	MHVA59	MHVA59	NNP
cord-253024-b393ea2u	183	11	ts	ts	NN
cord-253024-b393ea2u	183	12	mutants	mutant	NNS
cord-253024-b393ea2u	183	13	were	be	VBD
cord-253024-b393ea2u	183	14	amenable	amenable	JJ
cord-253024-b393ea2u	183	15	to	to	IN
cord-253024-b393ea2u	183	16	complementa-	complementa-	VB
cord-253024-b393ea2u	183	17	tion	tion	NN
cord-253024-b393ea2u	183	18	analysis	analysis	NN
cord-253024-b393ea2u	183	19	,	,	,
cord-253024-b393ea2u	183	20	providing	provide	VBG
cord-253024-b393ea2u	183	21	strong	strong	JJ
cord-253024-b393ea2u	183	22	evidence	evidence	NN
cord-253024-b393ea2u	183	23	that	that	IN
cord-253024-b393ea2u	183	24	all	all	DT
cord-253024-b393ea2u	183	25	of	of	IN
cord-253024-b393ea2u	183	26	the	the	DT
cord-253024-b393ea2u	183	27	RNA+	RNA+	NNP
cord-253024-b393ea2u	183	28	ts	ts	NN
cord-253024-b393ea2u	183	29	mutants	mutant	NNS
cord-253024-b393ea2u	183	30	used	use	VBN
cord-253024-b393ea2u	183	31	in	in	IN
cord-253024-b393ea2u	183	32	this	this	DT
cord-253024-b393ea2u	183	33	study	study	NN
cord-253024-b393ea2u	183	34	contain	contain	VBP
cord-253024-b393ea2u	183	35	defects	defect	NNS
cord-253024-b393ea2u	183	36	in	in	IN
cord-253024-b393ea2u	183	37	a	a	DT
cord-253024-b393ea2u	183	38	single	single	JJ
cord-253024-b393ea2u	183	39	gene	gene	NN
cord-253024-b393ea2u	183	40	or	or	CC
cord-253024-b393ea2u	183	41	a	a	DT
cord-253024-b393ea2u	183	42	noncomplementable	noncomplementable	JJ
cord-253024-b393ea2u	183	43	function	function	NN
cord-253024-b393ea2u	183	44	(	(	-LRB-
cord-253024-b393ea2u	183	45	Koolen	Koolen	NNP
cord-253024-b393ea2u	183	46	et	et	NNP
cord-253024-b393ea2u	183	47	al	al	NNP
cord-253024-b393ea2u	183	48	.	.	NNP
cord-253024-b393ea2u	183	49	,	,	,
cord-253024-b393ea2u	183	50	1983	1983	CD
cord-253024-b393ea2u	183	51	;	;	.
cord-253024-b393ea2u	184	1	Leibowitz	Leibowitz	NNP
cord-253024-b393ea2u	184	2	et	et	NNP
cord-253024-b393ea2u	184	3	al	al	NNP
cord-253024-b393ea2u	184	4	.	.	NNP
cord-253024-b393ea2u	184	5	,	,	,
cord-253024-b393ea2u	184	6	1982	1982	CD
cord-253024-b393ea2u	185	1	;	;	:
cord-253024-b393ea2u	185	2	Martin	Martin	NNP
cord-253024-b393ea2u	185	3	et	et	NNP
cord-253024-b393ea2u	185	4	al	al	NNP
cord-253024-b393ea2u	185	5	.	.	NNP
cord-253024-b393ea2u	185	6	,	,	,
cord-253024-b393ea2u	185	7	1988	1988	CD
cord-253024-b393ea2u	185	8	;	;	:
cord-253024-b393ea2u	185	9	Schaad	Schaad	NNP
cord-253024-b393ea2u	185	10	et	et	NNP
cord-253024-b393ea2u	185	11	al	al	NNP
cord-253024-b393ea2u	185	12	.	.	NNP
cord-253024-b393ea2u	185	13	,	,	,
cord-253024-b393ea2u	185	14	1990	1990	CD
cord-253024-b393ea2u	185	15	)	)	-RRB-
cord-253024-b393ea2u	185	16	.	.	.
cord-253024-b393ea2u	186	1	All	all	DT
cord-253024-b393ea2u	186	2	mutants	mutant	NNS
cord-253024-b393ea2u	186	3	were	be	VBD
cord-253024-b393ea2u	186	4	originally	originally	RB
cord-253024-b393ea2u	186	5	crossed	cross	VBN
cord-253024-b393ea2u	186	6	three	three	CD
cord-253024-b393ea2u	186	7	to	to	TO
cord-253024-b393ea2u	186	8	five	five	CD
cord-253024-b393ea2u	186	9	times	time	NNS
cord-253024-b393ea2u	186	10	with	with	IN
cord-253024-b393ea2u	186	11	the	the	DT
cord-253024-b393ea2u	186	12	reference	reference	NN
cord-253024-b393ea2u	186	13	mutants	mutant	NNS
cord-253024-b393ea2u	186	14	LA7	LA7	NNP
cord-253024-b393ea2u	186	15	,	,	,
cord-253024-b393ea2u	186	16	NC16	NC16	NNP
cord-253024-b393ea2u	186	17	,	,	,
cord-253024-b393ea2u	186	18	or	or	CC
cord-253024-b393ea2u	186	19	a	a	DT
cord-253024-b393ea2u	186	20	group	group	NN
cord-253024-b393ea2u	186	21	E	E	NNP
cord-253024-b393ea2u	186	22	RNA	rna	NN
cord-253024-b393ea2u	186	23	-	-	HYPH
cord-253024-b393ea2u	186	24	mutant	mutant	JJ
cord-253024-b393ea2u	186	25	LA18	LA18	NNP
cord-253024-b393ea2u	186	26	and	and	CC
cord-253024-b393ea2u	186	27	standardized	standardize	VBD
cord-253024-b393ea2u	186	28	to	to	IN
cord-253024-b393ea2u	186	29	the	the	DT
cord-253024-b393ea2u	186	30	reference	reference	NN
cord-253024-b393ea2u	186	31	cross	cross	NN
cord-253024-b393ea2u	186	32	LA7	LA7	NNP
cord-253024-b393ea2u	186	33	X	X	NNP
cord-253024-b393ea2u	186	34	NC16	NC16	NNP
cord-253024-b393ea2u	186	35	.	.	.
cord-253024-b393ea2u	187	1	To	to	TO
cord-253024-b393ea2u	187	2	obtain	obtain	VB
cord-253024-b393ea2u	187	3	a	a	DT
cord-253024-b393ea2u	187	4	more	more	RBR
cord-253024-b393ea2u	187	5	detailed	detailed	JJ
cord-253024-b393ea2u	187	6	map	map	NN
cord-253024-b393ea2u	187	7	of	of	IN
cord-253024-b393ea2u	187	8	the	the	DT
cord-253024-b393ea2u	187	9	group	group	NN
cord-253024-b393ea2u	187	10	F	F	NNP
cord-253024-b393ea2u	187	11	mutants	mutant	NNS
cord-253024-b393ea2u	187	12	,	,	,
cord-253024-b393ea2u	187	13	crosses	crosse	NNS
cord-253024-b393ea2u	187	14	were	be	VBD
cord-253024-b393ea2u	187	15	also	also	RB
cord-253024-b393ea2u	187	16	performed	perform	VBN
cord-253024-b393ea2u	187	17	among	among	IN
cord-253024-b393ea2u	187	18	each	each	DT
cord-253024-b393ea2u	187	19	of	of	IN
cord-253024-b393ea2u	187	20	the	the	DT
cord-253024-b393ea2u	187	21	viruses	virus	NNS
cord-253024-b393ea2u	187	22	used	use	VBN
cord-253024-b393ea2u	187	23	in	in	IN
cord-253024-b393ea2u	187	24	this	this	DT
cord-253024-b393ea2u	187	25	study	study	NN
cord-253024-b393ea2u	187	26	and	and	CC
cord-253024-b393ea2u	187	27	standardized	standardize	VBD
cord-253024-b393ea2u	187	28	to	to	IN
cord-253024-b393ea2u	187	29	the	the	DT
cord-253024-b393ea2u	187	30	reference	reference	NN
cord-253024-b393ea2u	187	31	cross	cross	NN
cord-253024-b393ea2u	187	32	.	.	.
cord-253024-b393ea2u	188	1	The	the	DT
cord-253024-b393ea2u	188	2	results	result	NNS
cord-253024-b393ea2u	188	3	of	of	IN
cord-253024-b393ea2u	188	4	these	these	DT
cord-253024-b393ea2u	188	5	experiments	experiment	NNS
cord-253024-b393ea2u	188	6	are	be	VBP
cord-253024-b393ea2u	188	7	shown	show	VBN
cord-253024-b393ea2u	188	8	in	in	IN
cord-253024-b393ea2u	188	9	Table	Table	NNP
cord-253024-b393ea2u	188	10	3	3	CD
cord-253024-b393ea2u	188	11	.	.	.
cord-253024-b393ea2u	189	1	The	the	DT
cord-253024-b393ea2u	189	2	distances	distance	NNS
cord-253024-b393ea2u	189	3	between	between	IN
cord-253024-b393ea2u	189	4	different	different	JJ
cord-253024-b393ea2u	189	5	ts	ts	NN
cord-253024-b393ea2u	189	6	mutants	mutant	NNS
cord-253024-b393ea2u	189	7	were	be	VBD
cord-253024-b393ea2u	189	8	within	within	IN
cord-253024-b393ea2u	189	9	statistical	statistical	JJ
cord-253024-b393ea2u	189	10	limits	limit	NNS
cord-253024-b393ea2u	189	11	and	and	CC
cord-253024-b393ea2u	189	12	permitted	permit	VBD
cord-253024-b393ea2u	189	13	the	the	DT
cord-253024-b393ea2u	189	14	construction	construction	NN
cord-253024-b393ea2u	189	15	of	of	IN
cord-253024-b393ea2u	189	16	a	a	DT
cord-253024-b393ea2u	189	17	genetic	genetic	JJ
cord-253024-b393ea2u	189	18	map	map	NN
cord-253024-b393ea2u	189	19	(	(	-LRB-
cord-253024-b393ea2u	189	20	Fig	Fig	NNP
cord-253024-b393ea2u	189	21	.	.	.
cord-253024-b393ea2u	189	22	3	3	CD
cord-253024-b393ea2u	189	23	)	)	-RRB-
cord-253024-b393ea2u	189	24	.	.	.
cord-253024-b393ea2u	190	1	On	on	IN
cord-253024-b393ea2u	190	2	the	the	DT
cord-253024-b393ea2u	190	3	basis	basis	NN
cord-253024-b393ea2u	190	4	of	of	IN
cord-253024-b393ea2u	190	5	their	-PRON-	PRP$
cord-253024-b393ea2u	190	6	genetic	genetic	JJ
cord-253024-b393ea2u	190	7	recombination	recombination	NN
cord-253024-b393ea2u	190	8	frequencies	frequency	NNS
cord-253024-b393ea2u	190	9	,	,	,
cord-253024-b393ea2u	190	10	the	the	DT
cord-253024-b393ea2u	190	11	group	group	NN
cord-253024-b393ea2u	190	12	F	F	NNP
cord-253024-b393ea2u	190	13	RNA+	RNA+	NNP
cord-253024-b393ea2u	190	14	ts	ts	NN
cord-253024-b393ea2u	190	15	mutants	mutant	NNS
cord-253024-b393ea2u	190	16	appeared	appear	VBD
cord-253024-b393ea2u	190	17	to	to	TO
cord-253024-b393ea2u	190	18	map	map	VB
cord-253024-b393ea2u	190	19	into	into	IN
cord-253024-b393ea2u	190	20	two	two	CD
cord-253024-b393ea2u	190	21	distinct	distinct	JJ
cord-253024-b393ea2u	190	22	domains	domain	NNS
cord-253024-b393ea2u	190	23	,	,	,
cord-253024-b393ea2u	190	24	designated	designate	VBN
cord-253024-b393ea2u	190	25	Fl	Fl	NNP
cord-253024-b393ea2u	190	26	(	(	-LRB-
cord-253024-b393ea2u	190	27	LA7	LA7	NNP
cord-253024-b393ea2u	190	28	,	,	,
cord-253024-b393ea2u	190	29	LA12	LA12	NNP
cord-253024-b393ea2u	190	30	,	,	,
cord-253024-b393ea2u	190	31	NC6	nc6	NN
cord-253024-b393ea2u	190	32	)	)	-RRB-
cord-253024-b393ea2u	190	33	and	and	CC
cord-253024-b393ea2u	190	34	F2	F2	NNP
cord-253024-b393ea2u	190	35	(	(	-LRB-
cord-253024-b393ea2u	190	36	NC14	NC14	NNP
cord-253024-b393ea2u	190	37	,	,	,
cord-253024-b393ea2u	190	38	NC16	NC16	NNP
cord-253024-b393ea2u	190	39	,	,	,
cord-253024-b393ea2u	190	40	NC17	NC17	NNP
cord-253024-b393ea2u	190	41	,	,	,
cord-253024-b393ea2u	190	42	LA1	LA1	NNP
cord-253024-b393ea2u	190	43	2	2	CD
cord-253024-b393ea2u	190	44	)	)	-RRB-
cord-253024-b393ea2u	190	45	.	.	.
cord-253024-b393ea2u	191	1	Ts	Ts	NNP
cord-253024-b393ea2u	191	2	LA1	LA1	NNP
cord-253024-b393ea2u	191	3	2	2	CD
cord-253024-b393ea2u	191	4	mapped	map	VBN
cord-253024-b393ea2u	191	5	closely	closely	RB
cord-253024-b393ea2u	191	6	to	to	IN
cord-253024-b393ea2u	191	7	representative	representative	JJ
cord-253024-b393ea2u	191	8	mu	mu	NNS
cord-253024-b393ea2u	191	9	-	-	HYPH
cord-253024-b393ea2u	191	10	tants	tant	NNS
cord-253024-b393ea2u	191	11	within	within	IN
cord-253024-b393ea2u	191	12	each	each	DT
cord-253024-b393ea2u	191	13	subgroup	subgroup	NN
cord-253024-b393ea2u	191	14	suggesting	suggest	VBG
cord-253024-b393ea2u	191	15	that	that	IN
cord-253024-b393ea2u	191	16	it	-PRON-	PRP
cord-253024-b393ea2u	191	17	may	may	MD
cord-253024-b393ea2u	191	18	be	be	VB
cord-253024-b393ea2u	191	19	a	a	DT
cord-253024-b393ea2u	191	20	double	double	JJ
cord-253024-b393ea2u	191	21	mutant	mutant	NN
cord-253024-b393ea2u	191	22	.	.	.
cord-253024-b393ea2u	192	1	Assuming	assume	VBG
cord-253024-b393ea2u	192	2	that	that	IN
cord-253024-b393ea2u	192	3	crossover	crossover	NN
cord-253024-b393ea2u	192	4	events	event	NNS
cord-253024-b393ea2u	192	5	occur	occur	VBP
cord-253024-b393ea2u	192	6	in	in	IN
cord-253024-b393ea2u	192	7	both	both	DT
cord-253024-b393ea2u	192	8	directions	direction	NNS
cord-253024-b393ea2u	192	9	and	and	CC
cord-253024-b393ea2u	192	10	that	that	IN
cord-253024-b393ea2u	192	11	NC6	NC6	NNP
cord-253024-b393ea2u	192	12	/	/	SYM
cord-253024-b393ea2u	192	13	LA7	LA7	NNP
cord-253024-b393ea2u	192	14	and	and	CC
cord-253024-b393ea2u	192	15	NC	NC	NNP
cord-253024-b393ea2u	192	16	14	14	CD
cord-253024-b393ea2u	192	17	/	/	SYM
cord-253024-b393ea2u	192	18	NC	NC	NNP
cord-253024-b393ea2u	192	19	16	16	CD
cord-253024-b393ea2u	192	20	map	map	NN
cord-253024-b393ea2u	192	21	at	at	IN
cord-253024-b393ea2u	192	22	different	different	JJ
cord-253024-b393ea2u	192	23	ends	end	NNS
cord-253024-b393ea2u	192	24	of	of	IN
cord-253024-b393ea2u	192	25	the	the	DT
cord-253024-b393ea2u	192	26	4.0-kb	4.0-kb	CD
cord-253024-b393ea2u	192	27	S	s	NN
cord-253024-b393ea2u	192	28	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	192	29	gene	gene	NN
cord-253024-b393ea2u	192	30	,	,	,
cord-253024-b393ea2u	192	31	a	a	DT
cord-253024-b393ea2u	192	32	1	1	CD
cord-253024-b393ea2u	192	33	%	%	NN
cord-253024-b393ea2u	192	34	recombination	recombination	NN
cord-253024-b393ea2u	192	35	frequency	frequency	NN
cord-253024-b393ea2u	192	36	occurred	occur	VBD
cord-253024-b393ea2u	192	37	over	over	IN
cord-253024-b393ea2u	192	38	575	575	CD
cord-253024-b393ea2u	192	39	-	-	SYM
cord-253024-b393ea2u	192	40	667	667	CD
cord-253024-b393ea2u	192	41	nucleotide	nucleotide	JJ
cord-253024-b393ea2u	192	42	pairs	pair	NNS
cord-253024-b393ea2u	192	43	of	of	IN
cord-253024-b393ea2u	192	44	RNA	RNA	NNP
cord-253024-b393ea2u	192	45	.	.	.
cord-253024-b393ea2u	193	1	Since	since	IN
cord-253024-b393ea2u	193	2	these	these	DT
cord-253024-b393ea2u	193	3	mutants	mutant	NNS
cord-253024-b393ea2u	193	4	do	do	VBP
cord-253024-b393ea2u	193	5	not	not	RB
cord-253024-b393ea2u	193	6	produce	produce	VB
cord-253024-b393ea2u	193	7	syncytium	syncytium	NN
cord-253024-b393ea2u	193	8	at	at	IN
cord-253024-b393ea2u	193	9	the	the	DT
cord-253024-b393ea2u	193	10	restrictive	restrictive	JJ
cord-253024-b393ea2u	193	11	temperature	temperature	NN
cord-253024-b393ea2u	193	12	(	(	-LRB-
cord-253024-b393ea2u	193	13	Fig	fig	NN
cord-253024-b393ea2u	193	14	.	.	.
cord-253024-b393ea2u	193	15	 	 	_SP
cord-253024-b393ea2u	194	1	1	1	LS
cord-253024-b393ea2u	194	2	)	)	-RRB-
cord-253024-b393ea2u	195	1	and	and	CC
cord-253024-b393ea2u	195	2	sequence	sequence	NN
cord-253024-b393ea2u	195	3	analysis	analysis	NN
cord-253024-b393ea2u	195	4	of	of	IN
cord-253024-b393ea2u	195	5	RNA	RNA	NNP
cord-253024-b393ea2u	195	6	recombinant	recombinant	JJ
cord-253024-b393ea2u	195	7	viruses	virus	NNS
cord-253024-b393ea2u	196	1	suggested	suggest	VBD
cord-253024-b393ea2u	196	2	that	that	IN
cord-253024-b393ea2u	196	3	the	the	DT
cord-253024-b393ea2u	196	4	defect	defect	NN
cord-253024-b393ea2u	196	5	in	in	IN
cord-253024-b393ea2u	196	6	LA7	la7	NN
cord-253024-b393ea2u	196	7	mapped	map	VBN
cord-253024-b393ea2u	196	8	within	within	IN
cord-253024-b393ea2u	196	9	the	the	DT
cord-253024-b393ea2u	196	10	first	first	JJ
cord-253024-b393ea2u	196	11	1	1	CD
cord-253024-b393ea2u	196	12	.l	.l	CD
cord-253024-b393ea2u	196	13	kb	kb	NNP
cord-253024-b393ea2u	196	14	of	of	IN
cord-253024-b393ea2u	196	15	the	the	DT
cord-253024-b393ea2u	196	16	S	S	NNP
cord-253024-b393ea2u	196	17	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	196	18	gene	gene	NN
cord-253024-b393ea2u	196	19	(	(	-LRB-
cord-253024-b393ea2u	196	20	Banner	Banner	NNP
cord-253024-b393ea2u	196	21	et	et	FW
cord-253024-b393ea2u	196	22	al	al	NNP
cord-253024-b393ea2u	196	23	.	.	NNP
cord-253024-b393ea2u	196	24	,	,	,
cord-253024-b393ea2u	196	25	1990	1990	CD
cord-253024-b393ea2u	196	26	;	;	:
cord-253024-b393ea2u	196	27	Keck	Keck	NNP
cord-253024-b393ea2u	196	28	eta/.	eta/.	CD
cord-253024-b393ea2u	196	29	,	,	,
cord-253024-b393ea2u	196	30	1987	1987	CD
cord-253024-b393ea2u	196	31	Keck	Keck	NNP
cord-253024-b393ea2u	196	32	eta/.	eta/.	NN
cord-253024-b393ea2u	196	33	,	,	,
cord-253024-b393ea2u	196	34	,	,	,
cord-253024-b393ea2u	197	1	1988a	1988a	CD
cord-253024-b393ea2u	197	2	Makino	Makino	NNP
cord-253024-b393ea2u	197	3	eta/.	eta/.	NN
cord-253024-b393ea2u	197	4	,	,	,
cord-253024-b393ea2u	198	1	1986b	1986b	CD
cord-253024-b393ea2u	198	2	Makino	Makino	NNP
cord-253024-b393ea2u	198	3	eta/.	eta/.	NN
cord-253024-b393ea2u	198	4	,	,	,
cord-253024-b393ea2u	198	5	,	,	,
cord-253024-b393ea2u	198	6	1987	1987	CD
cord-253024-b393ea2u	198	7	,	,	,
cord-253024-b393ea2u	198	8	these	these	DT
cord-253024-b393ea2u	198	9	data	datum	NNS
cord-253024-b393ea2u	198	10	provided	provide	VBD
cord-253024-b393ea2u	198	11	an	an	DT
cord-253024-b393ea2u	198	12	anchor	anchor	NN
cord-253024-b393ea2u	198	13	for	for	IN
cord-253024-b393ea2u	198	14	mapping	map	VBG
cord-253024-b393ea2u	198	15	the	the	DT
cord-253024-b393ea2u	198	16	location	location	NN
cord-253024-b393ea2u	198	17	of	of	IN
cord-253024-b393ea2u	198	18	the	the	DT
cord-253024-b393ea2u	198	19	remaining	remain	VBG
cord-253024-b393ea2u	198	20	group	group	NN
cord-253024-b393ea2u	198	21	F	F	NNP
cord-253024-b393ea2u	198	22	RNA+	RNA+	NNP
cord-253024-b393ea2u	198	23	mutants	mutant	NNS
cord-253024-b393ea2u	198	24	.	.	.
cord-253024-b393ea2u	199	1	These	these	DT
cord-253024-b393ea2u	199	2	data	datum	NNS
cord-253024-b393ea2u	199	3	predicted	predict	VBD
cord-253024-b393ea2u	199	4	that	that	IN
cord-253024-b393ea2u	199	5	the	the	DT
cord-253024-b393ea2u	199	6	Fl	Fl	NNP
cord-253024-b393ea2u	199	7	mutants	mutant	NNS
cord-253024-b393ea2u	199	8	map	map	VBP
cord-253024-b393ea2u	199	9	in	in	IN
cord-253024-b393ea2u	199	10	the	the	DT
cord-253024-b393ea2u	199	11	Sl	sl	NN
cord-253024-b393ea2u	199	12	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	199	13	while	while	IN
cord-253024-b393ea2u	199	14	the	the	DT
cord-253024-b393ea2u	199	15	F2	F2	NNP
cord-253024-b393ea2u	199	16	mutants	mutant	NNS
cord-253024-b393ea2u	199	17	map	map	VBP
cord-253024-b393ea2u	199	18	within	within	IN
cord-253024-b393ea2u	199	19	the	the	DT
cord-253024-b393ea2u	199	20	S2	s2	NN
cord-253024-b393ea2u	199	21	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	199	22	sequences	sequence	NNS
cord-253024-b393ea2u	199	23	(	(	-LRB-
cord-253024-b393ea2u	199	24	Fig	Fig	NNP
cord-253024-b393ea2u	199	25	.	.	NNP
cord-253024-b393ea2u	199	26	4	4	CD
cord-253024-b393ea2u	199	27	)	)	-RRB-
cord-253024-b393ea2u	199	28	.	.	.
cord-253024-b393ea2u	200	1	Comparisons	comparison	NNS
cord-253024-b393ea2u	200	2	between	between	IN
cord-253024-b393ea2u	200	3	the	the	DT
cord-253024-b393ea2u	200	4	recombination	recombination	NN
cord-253024-b393ea2u	200	5	frequencies	frequency	NNS
cord-253024-b393ea2u	200	6	in	in	IN
cord-253024-b393ea2u	200	7	the	the	DT
cord-253024-b393ea2u	200	8	polymerase	polymerase	NN
cord-253024-b393ea2u	200	9	and	and	CC
cord-253024-b393ea2u	200	10	S	S	NNP
cord-253024-b393ea2u	200	11	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	200	12	genes	gene	NNS
cord-253024-b393ea2u	200	13	Using	use	VBG
cord-253024-b393ea2u	200	14	well	well	RB
cord-253024-b393ea2u	200	15	characterized	characterize	VBN
cord-253024-b393ea2u	200	16	ts	ts	NN
cord-253024-b393ea2u	200	17	mutants	mutant	NNS
cord-253024-b393ea2u	200	18	of	of	IN
cord-253024-b393ea2u	200	19	MHV	MHV	NNP
cord-253024-b393ea2u	200	20	-	-	HYPH
cord-253024-b393ea2u	200	21	A59	A59	NNP
cord-253024-b393ea2u	200	22	,	,	,
cord-253024-b393ea2u	200	23	we	-PRON-	PRP
cord-253024-b393ea2u	200	24	have	have	VBP
cord-253024-b393ea2u	200	25	predicted	predict	VBN
cord-253024-b393ea2u	200	26	that	that	IN
cord-253024-b393ea2u	200	27	the	the	DT
cord-253024-b393ea2u	200	28	recombination	recombination	NN
cord-253024-b393ea2u	200	29	frequency	frequency	NN
cord-253024-b393ea2u	200	30	between	between	IN
cord-253024-b393ea2u	200	31	the	the	DT
cord-253024-b393ea2u	200	32	group	group	NN
cord-253024-b393ea2u	200	33	F	F	NNP
cord-253024-b393ea2u	200	34	RNA	RNA	NNP
cord-253024-b393ea2u	200	35	'	'	POS
cord-253024-b393ea2u	200	36	mutant	mutant	JJ
cord-253024-b393ea2u	200	37	LA7	la7	NN
cord-253024-b393ea2u	200	38	,	,	,
cord-253024-b393ea2u	200	39	and	and	CC
cord-253024-b393ea2u	200	40	the	the	DT
cord-253024-b393ea2u	200	41	group	group	NN
cord-253024-b393ea2u	201	1	A	a	DT
cord-253024-b393ea2u	201	2	RNA	RNA	NNP
cord-253024-b393ea2u	201	3	-	-	HYPH
cord-253024-b393ea2u	201	4	mutants	mutant	NNS
cord-253024-b393ea2u	201	5	LA6	LA6	NNP
cord-253024-b393ea2u	201	6	/	/	SYM
cord-253024-b393ea2u	201	7	LA3	LA3	NNP
cord-253024-b393ea2u	201	8	to	to	TO
cord-253024-b393ea2u	201	9	be	be	VB
cord-253024-b393ea2u	201	10	approximately	approximately	RB
cord-253024-b393ea2u	201	11	l%/	l%/	.
cord-253024-b393ea2u	202	1	1300-l	1300-l	LS
cord-253024-b393ea2u	202	2	400	400	CD
cord-253024-b393ea2u	202	3	nucleotides	nucleotide	NNS
cord-253024-b393ea2u	202	4	of	of	IN
cord-253024-b393ea2u	202	5	dsRNA	dsrna	NN
cord-253024-b393ea2u	203	1	(	(	-LRB-
cord-253024-b393ea2u	203	2	Fig	Fig	NNP
cord-253024-b393ea2u	203	3	.	.	NNP
cord-253024-b393ea2u	203	4	5	5	CD
cord-253024-b393ea2u	203	5	)	)	-RRB-
cord-253024-b393ea2u	203	6	.	.	.
cord-253024-b393ea2u	204	1	To	to	TO
cord-253024-b393ea2u	204	2	determine	determine	VB
cord-253024-b393ea2u	204	3	if	if	IN
cord-253024-b393ea2u	204	4	recombination	recombination	NN
cord-253024-b393ea2u	204	5	frequencies	frequency	NNS
cord-253024-b393ea2u	204	6	vary	vary	VBP
cord-253024-b393ea2u	204	7	in	in	IN
cord-253024-b393ea2u	204	8	different	different	JJ
cord-253024-b393ea2u	204	9	portions	portion	NNS
cord-253024-b393ea2u	204	10	of	of	IN
cord-253024-b393ea2u	204	11	the	the	DT
cord-253024-b393ea2u	204	12	genome	genome	NN
cord-253024-b393ea2u	204	13	,	,	,
cord-253024-b393ea2u	204	14	we	-PRON-	PRP
cord-253024-b393ea2u	204	15	compared	compare	VBD
cord-253024-b393ea2u	204	16	the	the	DT
cord-253024-b393ea2u	204	17	maximum	maximum	JJ
cord-253024-b393ea2u	204	18	recombination	recombination	NN
cord-253024-b393ea2u	204	19	frequencies	frequency	NNS
cord-253024-b393ea2u	204	20	between	between	IN
cord-253024-b393ea2u	204	21	mutants	mutant	NNS
cord-253024-b393ea2u	204	22	spanning	span	VBG
cord-253024-b393ea2u	204	23	the	the	DT
cord-253024-b393ea2u	204	24	polymerase	polymerase	NN
cord-253024-b393ea2u	204	25	genes	gene	NNS
cord-253024-b393ea2u	204	26	of	of	IN
cord-253024-b393ea2u	204	27	MHV	MHV	NNP
cord-253024-b393ea2u	205	1	(	(	-LRB-
cord-253024-b393ea2u	205	2	Group	Group	NNP
cord-253024-b393ea2u	205	3	A	A	NNP
cord-253024-b393ea2u	206	1	(	(	-LRB-
cord-253024-b393ea2u	206	2	LA3	LA3	NNP
cord-253024-b393ea2u	206	3	/	/	SYM
cord-253024-b393ea2u	206	4	LA6	LA6	NNP
cord-253024-b393ea2u	206	5	)	)	-RRB-
cord-253024-b393ea2u	207	1	X	X	NNP
cord-253024-b393ea2u	207	2	Group	Group	NNP
cord-253024-b393ea2u	207	3	E	E	NNP
cord-253024-b393ea2u	207	4	(	(	-LRB-
cord-253024-b393ea2u	207	5	LA1	LA1	NNP
cord-253024-b393ea2u	207	6	8)	8)	NNP
cord-253024-b393ea2u	207	7	)	)	-RRB-
cord-253024-b393ea2u	208	1	across	across	IN
cord-253024-b393ea2u	208	2	gene	gene	NN
cord-253024-b393ea2u	208	3	B	B	NNP
cord-253024-b393ea2u	208	4	encoding	encode	VBG
cord-253024-b393ea2u	208	5	the	the	DT
cord-253024-b393ea2u	208	6	p30	p30	NN
cord-253024-b393ea2u	208	7	and	and	CC
cord-253024-b393ea2u	208	8	HE	HE	NNP
cord-253024-b393ea2u	208	9	genes	gene	NNS
cord-253024-b393ea2u	208	10	(	(	-LRB-
cord-253024-b393ea2u	208	11	Group	group	NN
cord-253024-b393ea2u	208	12	E	E	NNP
cord-253024-b393ea2u	208	13	(	(	-LRB-
cord-253024-b393ea2u	208	14	LA18	LA18	NNP
cord-253024-b393ea2u	208	15	)	)	-RRB-
cord-253024-b393ea2u	208	16	X	X	NNP
cord-253024-b393ea2u	208	17	Group	Group	NNP
cord-253024-b393ea2u	208	18	F	F	NNP
cord-253024-b393ea2u	208	19	(	(	-LRB-
cord-253024-b393ea2u	208	20	LA7	LA7	NNP
cord-253024-b393ea2u	208	21	/	/	SYM
cord-253024-b393ea2u	208	22	LA12	LA12	NNP
cord-253024-b393ea2u	208	23	)	)	-RRB-
cord-253024-b393ea2u	208	24	)	)	-RRB-
cord-253024-b393ea2u	208	25	or	or	CC
cord-253024-b393ea2u	208	26	through	through	IN
cord-253024-b393ea2u	208	27	the	the	DT
cord-253024-b393ea2u	208	28	S	S	NNP
cord-253024-b393ea2u	208	29	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	208	30	gene	gene	NN
cord-253024-b393ea2u	208	31	(	(	-LRB-
cord-253024-b393ea2u	208	32	Group	Group	NNP
cord-253024-b393ea2u	208	33	F	F	NNP
cord-253024-b393ea2u	208	34	(	(	-LRB-
cord-253024-b393ea2u	208	35	LA7	LA7	NNP
cord-253024-b393ea2u	208	36	X	X	NNP
cord-253024-b393ea2u	208	37	NC16INC14	NC16INC14	NNP
cord-253024-b393ea2u	208	38	)	)	-RRB-
cord-253024-b393ea2u	208	39	)	)	-RRB-
cord-253024-b393ea2u	208	40	.	.	.
cord-253024-b393ea2u	209	1	All	all	DT
cord-253024-b393ea2u	209	2	mutants	mutant	NNS
cord-253024-b393ea2u	209	3	were	be	VBD
cord-253024-b393ea2u	209	4	crossed	cross	VBN
cord-253024-b393ea2u	209	5	three	three	CD
cord-253024-b393ea2u	209	6	to	to	TO
cord-253024-b393ea2u	209	7	five	five	CD
cord-253024-b393ea2u	209	8	times	time	NNS
cord-253024-b393ea2u	209	9	and	and	CC
cord-253024-b393ea2u	209	10	average	average	JJ
cord-253024-b393ea2u	209	11	recombination	recombination	NN
cord-253024-b393ea2u	209	12	frequencies	frequency	NNS
cord-253024-b393ea2u	209	13	calculated	calculate	VBN
cord-253024-b393ea2u	209	14	as	as	IN
cord-253024-b393ea2u	209	15	previously	previously	RB
cord-253024-b393ea2u	209	16	described	describe	VBN
cord-253024-b393ea2u	209	17	(	(	-LRB-
cord-253024-b393ea2u	209	18	Fig	Fig	NNP
cord-253024-b393ea2u	209	19	.	.	.
cord-253024-b393ea2u	209	20	5	5	CD
cord-253024-b393ea2u	209	21	)	)	-RRB-
cord-253024-b393ea2u	209	22	.	.	.
cord-253024-b393ea2u	210	1	Interestingly	interestingly	RB
cord-253024-b393ea2u	210	2	,	,	,
cord-253024-b393ea2u	210	3	the	the	DT
cord-253024-b393ea2u	210	4	maximum	maximum	JJ
cord-253024-b393ea2u	210	5	recombination	recombination	NN
cord-253024-b393ea2u	210	6	frequency	frequency	NN
cord-253024-b393ea2u	210	7	predicted	predict	VBN
cord-253024-b393ea2u	210	8	between	between	IN
cord-253024-b393ea2u	210	9	the	the	DT
cord-253024-b393ea2u	210	10	two	two	CD
cord-253024-b393ea2u	210	11	most	most	RBS
cord-253024-b393ea2u	210	12	distant	distant	JJ
cord-253024-b393ea2u	210	13	ts	ts	NN
cord-253024-b393ea2u	210	14	mutants	mutant	NNS
cord-253024-b393ea2u	210	15	in	in	IN
cord-253024-b393ea2u	210	16	complementation	complementation	NN
cord-253024-b393ea2u	210	17	groups	group	NNS
cord-253024-b393ea2u	211	1	A	a	DT
cord-253024-b393ea2u	212	1	(	(	-LRB-
cord-253024-b393ea2u	212	2	LA3	LA3	NNP
cord-253024-b393ea2u	212	3	,	,	,
cord-253024-b393ea2u	212	4	LA6	LA6	NNP
cord-253024-b393ea2u	212	5	)	)	-RRB-
cord-253024-b393ea2u	212	6	and	and	CC
cord-253024-b393ea2u	212	7	E	E	NNP
cord-253024-b393ea2u	212	8	(	(	-LRB-
cord-253024-b393ea2u	212	9	LA18	LA18	NNP
cord-253024-b393ea2u	212	10	)	)	-RRB-
cord-253024-b393ea2u	212	11	in	in	IN
cord-253024-b393ea2u	212	12	the	the	DT
cord-253024-b393ea2u	212	13	22-kb	22-kb	CD
cord-253024-b393ea2u	212	14	polymerase	polymerase	NN
cord-253024-b393ea2u	212	15	region	region	NN
cord-253024-b393ea2u	212	16	ranged	range	VBD
cord-253024-b393ea2u	212	17	from	from	IN
cord-253024-b393ea2u	212	18	about	about	RB
cord-253024-b393ea2u	212	19	1%/l	1%/l	CD
cord-253024-b393ea2u	212	20	800	800	CD
cord-253024-b393ea2u	213	1	(	(	-LRB-
cord-253024-b393ea2u	213	2	LA1	LA1	NNP
cord-253024-b393ea2u	213	3	8	8	CD
cord-253024-b393ea2u	213	4	X	X	NNP
cord-253024-b393ea2u	213	5	LA3	LA3	NNS
cord-253024-b393ea2u	213	6	)	)	-RRB-
cord-253024-b393ea2u	213	7	to	to	IN
cord-253024-b393ea2u	213	8	1	1	CD
cord-253024-b393ea2u	213	9	%	%	NN
cord-253024-b393ea2u	214	1	I2500	I2500	NNP
cord-253024-b393ea2u	215	1	(	(	-LRB-
cord-253024-b393ea2u	215	2	LA1	LA1	NNP
cord-253024-b393ea2u	215	3	8	8	CD
cord-253024-b393ea2u	215	4	X	X	NNP
cord-253024-b393ea2u	215	5	LA6	LA6	NNP
cord-253024-b393ea2u	215	6	)	)	-RRB-
cord-253024-b393ea2u	215	7	nucleotides	nucleotide	NNS
cord-253024-b393ea2u	215	8	of	of	IN
cord-253024-b393ea2u	215	9	ds	ds	NNP
cord-253024-b393ea2u	215	10	RNA	RNA	NNP
cord-253024-b393ea2u	215	11	.	.	.
cord-253024-b393ea2u	216	1	The	the	DT
cord-253024-b393ea2u	216	2	maximum	maximum	JJ
cord-253024-b393ea2u	216	3	recombination	recombination	NN
cord-253024-b393ea2u	216	4	frequency	frequency	NN
cord-253024-b393ea2u	216	5	predicted	predict	VBN
cord-253024-b393ea2u	216	6	between	between	IN
cord-253024-b393ea2u	216	7	the	the	DT
cord-253024-b393ea2u	216	8	group	group	NN
cord-253024-b393ea2u	216	9	E	e	NN
cord-253024-b393ea2u	216	10	and	and	CC
cord-253024-b393ea2u	216	11	F	F	NNP
cord-253024-b393ea2u	216	12	mutants	mutant	NNS
cord-253024-b393ea2u	216	13	that	that	WDT
cord-253024-b393ea2u	216	14	spanned	span	VBD
cord-253024-b393ea2u	216	15	the	the	DT
cord-253024-b393ea2u	216	16	p30	p30	NN
cord-253024-b393ea2u	216	17	and	and	CC
cord-253024-b393ea2u	216	18	HE	HE	NNP
cord-253024-b393ea2u	216	19	genes	gene	NNS
cord-253024-b393ea2u	216	20	ranged	range	VBD
cord-253024-b393ea2u	216	21	from	from	IN
cord-253024-b393ea2u	216	22	about	about	RB
cord-253024-b393ea2u	216	23	1%/l	1%/l	CD
cord-253024-b393ea2u	216	24	100	100	CD
cord-253024-b393ea2u	217	1	(	(	-LRB-
cord-253024-b393ea2u	217	2	LA1	LA1	NNP
cord-253024-b393ea2u	217	3	2	2	NNP
cord-253024-b393ea2u	217	4	X	X	NNP
cord-253024-b393ea2u	217	5	LA1	la1	NN
cord-253024-b393ea2u	217	6	8)	8)	NN
cord-253024-b393ea2u	217	7	to	to	IN
cord-253024-b393ea2u	217	8	1	1	CD
cord-253024-b393ea2u	217	9	%	%	NN
cord-253024-b393ea2u	218	1	/1400	/1400	NNP
cord-253024-b393ea2u	218	2	(	(	-LRB-
cord-253024-b393ea2u	218	3	LA7	LA7	NNP
cord-253024-b393ea2u	218	4	X	X	NNP
cord-253024-b393ea2u	218	5	LA18	LA18	NNP
cord-253024-b393ea2u	218	6	)	)	-RRB-
cord-253024-b393ea2u	218	7	nucleotides	nucleotide	NNS
cord-253024-b393ea2u	218	8	of	of	IN
cord-253024-b393ea2u	218	9	dsRNA	dsrna	NN
cord-253024-b393ea2u	218	10	.	.	.
cord-253024-b393ea2u	219	1	Since	since	IN
cord-253024-b393ea2u	219	2	the	the	DT
cord-253024-b393ea2u	219	3	maximum	maximum	JJ
cord-253024-b393ea2u	219	4	predicted	predict	VBN
cord-253024-b393ea2u	219	5	recombination	recombination	NN
cord-253024-b393ea2u	219	6	frequency	frequency	NN
cord-253024-b393ea2u	219	7	across	across	IN
cord-253024-b393ea2u	219	8	the	the	DT
cord-253024-b393ea2u	219	9	4.0-kb	4.0-kb	CD
cord-253024-b393ea2u	219	10	S	s	NN
cord-253024-b393ea2u	219	11	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	219	12	gene	gene	NN
cord-253024-b393ea2u	219	13	ranged	range	VBD
cord-253024-b393ea2u	219	14	from	from	IN
cord-253024-b393ea2u	219	15	about	about	RB
cord-253024-b393ea2u	219	16	1	1	CD
cord-253024-b393ea2u	219	17	o/0/575	o/0/575	NN
cord-253024-b393ea2u	220	1	(	(	-LRB-
cord-253024-b393ea2u	220	2	LA7	LA7	NNP
cord-253024-b393ea2u	220	3	x	x	SYM
cord-253024-b393ea2u	220	4	NC	NC	NNP
cord-253024-b393ea2u	220	5	14	14	CD
cord-253024-b393ea2u	220	6	)	)	-RRB-
cord-253024-b393ea2u	220	7	to	to	IN
cord-253024-b393ea2u	220	8	1	1	CD
cord-253024-b393ea2u	220	9	O	o	NN
cord-253024-b393ea2u	220	10	/	/	SYM
cord-253024-b393ea2u	220	11	o/667	o/667	VBN
cord-253024-b393ea2u	221	1	(	(	-LRB-
cord-253024-b393ea2u	221	2	LA7	LA7	NNP
cord-253024-b393ea2u	221	3	X	X	NNP
cord-253024-b393ea2u	221	4	NC	NC	NNP
cord-253024-b393ea2u	221	5	16	16	CD
cord-253024-b393ea2u	221	6	)	)	-RRB-
cord-253024-b393ea2u	221	7	nucleotides	nucleotide	NNS
cord-253024-b393ea2u	221	8	of	of	IN
cord-253024-b393ea2u	221	9	dsRNA	dsrna	NN
cord-253024-b393ea2u	221	10	,	,	,
cord-253024-b393ea2u	221	11	these	these	DT
cord-253024-b393ea2u	221	12	data	datum	NNS
cord-253024-b393ea2u	221	13	suggested	suggest	VBD
cord-253024-b393ea2u	221	14	that	that	IN
cord-253024-b393ea2u	221	15	variable	variable	JJ
cord-253024-b393ea2u	221	16	rates	rate	NNS
cord-253024-b393ea2u	221	17	of	of	IN
cord-253024-b393ea2u	221	18	recombination	recombination	NN
cord-253024-b393ea2u	221	19	occurwithin	occurwithin	JJ
cord-253024-b393ea2u	221	20	different	different	JJ
cord-253024-b393ea2u	221	21	portions	portion	NNS
cord-253024-b393ea2u	221	22	of	of	IN
cord-253024-b393ea2u	221	23	the	the	DT
cord-253024-b393ea2u	221	24	MHV	MHV	NNP
cord-253024-b393ea2u	221	25	genome	genome	NN
cord-253024-b393ea2u	221	26	(	(	-LRB-
cord-253024-b393ea2u	221	27	Figure	figure	NN
cord-253024-b393ea2u	221	28	5	5	CD
cord-253024-b393ea2u	221	29	)	)	-RRB-
cord-253024-b393ea2u	221	30	.	.	.
cord-253024-b393ea2u	222	1	Are	be	VBP
cord-253024-b393ea2u	222	2	deletions	deletion	NNS
cord-253024-b393ea2u	222	3	contributing	contribute	VBG
cord-253024-b393ea2u	222	4	to	to	IN
cord-253024-b393ea2u	222	5	the	the	DT
cord-253024-b393ea2u	222	6	increased	increase	VBN
cord-253024-b393ea2u	222	7	rates	rate	NNS
cord-253024-b393ea2u	222	8	of	of	IN
cord-253024-b393ea2u	222	9	recombination	recombination	NN
cord-253024-b393ea2u	222	10	among	among	IN
cord-253024-b393ea2u	222	11	the	the	DT
cord-253024-b393ea2u	222	12	group	group	NN
cord-253024-b393ea2u	222	13	F	F	NNP
cord-253024-b393ea2u	222	14	RNA+	RNA+	NNP
cord-253024-b393ea2u	222	15	mutants	mutant	NNS
cord-253024-b393ea2u	222	16	?	?	.
cord-253024-b393ea2u	223	1	Extensive	extensive	JJ
cord-253024-b393ea2u	223	2	amounts	amount	NNS
cord-253024-b393ea2u	223	3	of	of	IN
cord-253024-b393ea2u	223	4	polymorphism	polymorphism	NN
cord-253024-b393ea2u	223	5	and	and	CC
cord-253024-b393ea2u	223	6	deletion	deletion	NN
cord-253024-b393ea2u	223	7	have	have	VBP
cord-253024-b393ea2u	223	8	been	be	VBN
cord-253024-b393ea2u	223	9	detected	detect	VBN
cord-253024-b393ea2u	223	10	in	in	IN
cord-253024-b393ea2u	223	11	both	both	CC
cord-253024-b393ea2u	223	12	the	the	DT
cord-253024-b393ea2u	223	13	HE	HE	NNP
cord-253024-b393ea2u	223	14	and	and	CC
cord-253024-b393ea2u	223	15	the	the	DT
cord-253024-b393ea2u	223	16	S	S	NNP
cord-253024-b393ea2u	223	17	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	223	18	gene	gene	NN
cord-253024-b393ea2u	223	19	sequences	sequence	NNS
cord-253024-b393ea2u	223	20	of	of	IN
cord-253024-b393ea2u	223	21	different	different	JJ
cord-253024-b393ea2u	223	22	MHV	MHV	NNP
cord-253024-b393ea2u	223	23	strains	strain	NNS
cord-253024-b393ea2u	223	24	and	and	CC
cord-253024-b393ea2u	223	25	in	in	IN
cord-253024-b393ea2u	223	26	the	the	DT
cord-253024-b393ea2u	223	27	sequence	sequence	NN
cord-253024-b393ea2u	223	28	of	of	IN
cord-253024-b393ea2u	223	29	other	other	JJ
cord-253024-b393ea2u	223	30	coronaviruses	coronaviruse	NNS
cord-253024-b393ea2u	224	1	(	(	-LRB-
cord-253024-b393ea2u	224	2	Banner	Banner	NNP
cord-253024-b393ea2u	224	3	et	et	FW
cord-253024-b393ea2u	224	4	al	al	NNP
cord-253024-b393ea2u	224	5	.	.	.
cord-253024-b393ea2u	225	1	,	,	,
cord-253024-b393ea2u	225	2	1990	1990	CD
cord-253024-b393ea2u	225	3	;	;	:
cord-253024-b393ea2u	225	4	Boireau	Boireau	NNP
cord-253024-b393ea2u	225	5	et	et	NNP
cord-253024-b393ea2u	225	6	a/.	a/.	NN
cord-253024-b393ea2u	225	7	,	,	,
cord-253024-b393ea2u	226	1	1990	1990	CD
cord-253024-b393ea2u	226	2	Parker	Parker	NNP
cord-253024-b393ea2u	226	3	et	et	NNP
cord-253024-b393ea2u	226	4	al	al	NNP
cord-253024-b393ea2u	226	5	.	.	.
cord-253024-b393ea2u	227	1	,	,	,
cord-253024-b393ea2u	227	2	1990	1990	CD
cord-253024-b393ea2u	227	3	;	;	:
cord-253024-b393ea2u	227	4	Rasschaert	Rasschaert	NNP
cord-253024-b393ea2u	227	5	et	et	NNP
cord-253024-b393ea2u	227	6	a/.	a/.	NNP
cord-253024-b393ea2u	227	7	,	,	,
cord-253024-b393ea2u	227	8	1990	1990	CD
cord-253024-b393ea2u	227	9	)	)	-RRB-
cord-253024-b393ea2u	227	10	.	.	.
cord-253024-b393ea2u	228	1	It	-PRON-	PRP
cord-253024-b393ea2u	228	2	is	be	VBZ
cord-253024-b393ea2u	228	3	possible	possible	JJ
cord-253024-b393ea2u	228	4	that	that	IN
cord-253024-b393ea2u	228	5	the	the	DT
cord-253024-b393ea2u	228	6	increased	increase	VBN
cord-253024-b393ea2u	228	7	rate	rate	NN
cord-253024-b393ea2u	228	8	of	of	IN
cord-253024-b393ea2u	228	9	recombination	recombination	NN
cord-253024-b393ea2u	228	10	within	within	IN
cord-253024-b393ea2u	228	11	the	the	DT
cord-253024-b393ea2u	228	12	S	S	NNP
cord-253024-b393ea2u	228	13	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	228	14	gene	gene	NN
cord-253024-b393ea2u	228	15	may	may	MD
cord-253024-b393ea2u	228	16	represent	represent	VB
cord-253024-b393ea2u	228	17	the	the	DT
cord-253024-b393ea2u	228	18	sum	sum	NN
cord-253024-b393ea2u	228	19	of	of	IN
cord-253024-b393ea2u	228	20	homologous	homologous	JJ
cord-253024-b393ea2u	228	21	recombination	recombination	NN
cord-253024-b393ea2u	228	22	and	and	CC
cord-253024-b393ea2u	228	23	recombination	recombination	NN
cord-253024-b393ea2u	228	24	(	(	-LRB-
cord-253024-b393ea2u	228	25	homologous	homologous	JJ
cord-253024-b393ea2u	228	26	or	or	CC
cord-253024-b393ea2u	228	27	nonhomologous	nonhomologous	NNP
cord-253024-b393ea2u	228	28	)	)	-RRB-
cord-253024-b393ea2u	228	29	resulting	result	VBG
cord-253024-b393ea2u	228	30	in	in	IN
cord-253024-b393ea2u	228	31	deletions	deletion	NNS
cord-253024-b393ea2u	228	32	.	.	.
cord-253024-b393ea2u	229	1	To	to	TO
cord-253024-b393ea2u	229	2	test	test	VB
cord-253024-b393ea2u	229	3	this	this	DT
cord-253024-b393ea2u	229	4	possibility	possibility	NN
cord-253024-b393ea2u	229	5	,	,	,
cord-253024-b393ea2u	229	6	cultures	culture	NNS
cord-253024-b393ea2u	229	7	of	of	IN
cord-253024-b393ea2u	229	8	cells	cell	NNS
cord-253024-b393ea2u	229	9	were	be	VBD
cord-253024-b393ea2u	229	10	infected	infect	VBN
cord-253024-b393ea2u	229	11	with	with	IN
cord-253024-b393ea2u	229	12	various	various	JJ
cord-253024-b393ea2u	229	13	combinations	combination	NNS
cord-253024-b393ea2u	229	14	of	of	IN
cord-253024-b393ea2u	229	15	ts	ts	JJ
cord-253024-b393ea2u	229	16	mutants	mutant	NNS
cord-253024-b393ea2u	229	17	as	as	IN
cord-253024-b393ea2u	229	18	shown	show	VBN
cord-253024-b393ea2u	229	19	in	in	IN
cord-253024-b393ea2u	229	20	Fig	Fig	NNP
cord-253024-b393ea2u	229	21	.	.	.
cord-253024-b393ea2u	230	1	4	4	LS
cord-253024-b393ea2u	230	2	and	and	CC
cord-253024-b393ea2u	230	3	RNA	RNA	NNP
cord-253024-b393ea2u	230	4	recombinant	recombinant	JJ
cord-253024-b393ea2u	230	5	viruses	virus	NNS
cord-253024-b393ea2u	230	6	isolated	isolate	VBN
cord-253024-b393ea2u	230	7	14	14	CD
cord-253024-b393ea2u	230	8	hr	hr	NN
cord-253024-b393ea2u	230	9	postinfection	postinfection	NN
cord-253024-b393ea2u	230	10	.	.	.
cord-253024-b393ea2u	231	1	Since	since	IN
cord-253024-b393ea2u	231	2	the	the	DT
cord-253024-b393ea2u	231	3	ratio	ratio	NN
cord-253024-b393ea2u	231	4	of	of	IN
cord-253024-b393ea2u	231	5	recombinants	recombinant	NNS
cord-253024-b393ea2u	231	6	/	/	SYM
cord-253024-b393ea2u	231	7	revertants	revertant	NNS
cord-253024-b393ea2u	231	8	ranged	range	VBD
cord-253024-b393ea2u	231	9	from	from	IN
cord-253024-b393ea2u	231	10	9	9	CD
cord-253024-b393ea2u	231	11	to	to	IN
cord-253024-b393ea2u	231	12	3	3	CD
cord-253024-b393ea2u	231	13	140	140	CD
cord-253024-b393ea2u	231	14	in	in	IN
cord-253024-b393ea2u	231	15	these	these	DT
cord-253024-b393ea2u	231	16	crosses	crosse	NNS
cord-253024-b393ea2u	231	17	(	(	-LRB-
cord-253024-b393ea2u	231	18	Table	table	NN
cord-253024-b393ea2u	231	19	2	2	CD
cord-253024-b393ea2u	231	20	)	)	-RRB-
cord-253024-b393ea2u	232	1	these	these	DT
cord-253024-b393ea2u	232	2	data	datum	NNS
cord-253024-b393ea2u	232	3	suggested	suggest	VBD
cord-253024-b393ea2u	232	4	that	that	IN
cord-253024-b393ea2u	232	5	the	the	DT
cord-253024-b393ea2u	232	6	majority	majority	NN
cord-253024-b393ea2u	232	7	of	of	IN
cord-253024-b393ea2u	232	8	ts+	ts+	NNP
cord-253024-b393ea2u	232	9	isolates	isolate	NNS
cord-253024-b393ea2u	232	10	represented	represent	VBD
cord-253024-b393ea2u	232	11	true	true	JJ
cord-253024-b393ea2u	232	12	RNA	RNA	NNP
cord-253024-b393ea2u	232	13	recombinants	recombinant	NNS
cord-253024-b393ea2u	232	14	and	and	CC
cord-253024-b393ea2u	232	15	not	not	RB
cord-253024-b393ea2u	232	16	revertants	revertant	NNS
cord-253024-b393ea2u	232	17	.	.	.
cord-253024-b393ea2u	233	1	Based	base	VBN
cord-253024-b393ea2u	233	2	on	on	IN
cord-253024-b393ea2u	233	3	the	the	DT
cord-253024-b393ea2u	233	4	recombination	recombination	NN
cord-253024-b393ea2u	233	5	rate	rate	NN
cord-253024-b393ea2u	233	6	in	in	IN
cord-253024-b393ea2u	233	7	the	the	DT
cord-253024-b393ea2u	233	8	S	S	NNP
cord-253024-b393ea2u	233	9	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	233	10	gene	gene	NN
cord-253024-b393ea2u	233	11	,	,	,
cord-253024-b393ea2u	233	12	the	the	DT
cord-253024-b393ea2u	233	13	predicted	predict	VBN
cord-253024-b393ea2u	233	14	distance	distance	NN
cord-253024-b393ea2u	233	15	between	between	IN
cord-253024-b393ea2u	233	16	the	the	DT
cord-253024-b393ea2u	233	17	ts	ts	NNP
cord-253024-b393ea2u	233	18	alleles	allele	NNS
cord-253024-b393ea2u	233	19	used	use	VBN
cord-253024-b393ea2u	233	20	in	in	IN
cord-253024-b393ea2u	233	21	these	these	DT
cord-253024-b393ea2u	233	22	crosses	crosse	NNS
cord-253024-b393ea2u	233	23	ranged	range	VBD
cord-253024-b393ea2u	233	24	from	from	IN
cord-253024-b393ea2u	233	25	-100	-100	NNP
cord-253024-b393ea2u	233	26	bp	bp	NNP
cord-253024-b393ea2u	233	27	to	to	IN
cord-253024-b393ea2u	233	28	>	>	XX
cord-253024-b393ea2u	233	29	l	l	NN
cord-253024-b393ea2u	233	30	000	000	CD
cord-253024-b393ea2u	233	31	nucleotides	nucleotide	NNS
cord-253024-b393ea2u	233	32	(	(	-LRB-
cord-253024-b393ea2u	233	33	Fig	Fig	NNP
cord-253024-b393ea2u	233	34	.	.	NNP
cord-253024-b393ea2u	233	35	4	4	CD
cord-253024-b393ea2u	233	36	)	)	-RRB-
cord-253024-b393ea2u	233	37	.	.	.
cord-253024-b393ea2u	234	1	To	to	TO
cord-253024-b393ea2u	234	2	determine	determine	VB
cord-253024-b393ea2u	234	3	if	if	IN
cord-253024-b393ea2u	234	4	deletions	deletion	NNS
cord-253024-b393ea2u	234	5	were	be	VBD
cord-253024-b393ea2u	234	6	present	present	JJ
cord-253024-b393ea2u	234	7	within	within	IN
cord-253024-b393ea2u	234	8	the	the	DT
cord-253024-b393ea2u	234	9	S	S	NNP
cord-253024-b393ea2u	234	10	gene	gene	NN
cord-253024-b393ea2u	234	11	of	of	IN
cord-253024-b393ea2u	234	12	RNA	RNA	NNP
cord-253024-b393ea2u	234	13	recombinant	recombinant	JJ
cord-253024-b393ea2u	234	14	viruses	virus	NNS
cord-253024-b393ea2u	234	15	,	,	,
cord-253024-b393ea2u	234	16	a	a	DT
cord-253024-b393ea2u	234	17	series	series	NN
cord-253024-b393ea2u	234	18	of	of	IN
cord-253024-b393ea2u	234	19	overlapping	overlap	VBG
cord-253024-b393ea2u	234	20	primers	primer	NNS
cord-253024-b393ea2u	234	21	were	be	VBD
cord-253024-b393ea2u	234	22	synthesized	synthesize	VBN
cord-253024-b393ea2u	234	23	that	that	WDT
cord-253024-b393ea2u	234	24	span	span	VBP
cord-253024-b393ea2u	234	25	the	the	DT
cord-253024-b393ea2u	234	26	entire	entire	JJ
cord-253024-b393ea2u	234	27	S	S	NNP
cord-253024-b393ea2u	234	28	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	234	29	gene	gene	NN
cord-253024-b393ea2u	234	30	of	of	IN
cord-253024-b393ea2u	234	31	MHV	MHV	NNP
cord-253024-b393ea2u	234	32	-	-	HYPH
cord-253024-b393ea2u	234	33	A59	A59	NNP
cord-253024-b393ea2u	234	34	.	.	.
cord-253024-b393ea2u	235	1	The	the	DT
cord-253024-b393ea2u	235	2	location	location	NN
cord-253024-b393ea2u	235	3	of	of	IN
cord-253024-b393ea2u	235	4	these	these	DT
cord-253024-b393ea2u	235	5	primers	primer	NNS
cord-253024-b393ea2u	235	6	as	as	RB
cord-253024-b393ea2u	235	7	well	well	RB
cord-253024-b393ea2u	235	8	as	as	IN
cord-253024-b393ea2u	235	9	the	the	DT
cord-253024-b393ea2u	235	10	size	size	NN
cord-253024-b393ea2u	235	11	of	of	IN
cord-253024-b393ea2u	235	12	predicted	predict	VBN
cord-253024-b393ea2u	235	13	PCR	PCR	NNP
cord-253024-b393ea2u	235	14	products	product	NNS
cord-253024-b393ea2u	235	15	are	be	VBP
cord-253024-b393ea2u	235	16	shown	show	VBN
cord-253024-b393ea2u	235	17	in	in	IN
cord-253024-b393ea2u	235	18	Fig	Fig	NNP
cord-253024-b393ea2u	235	19	.	.	.
cord-253024-b393ea2u	236	1	4	4	LS
cord-253024-b393ea2u	236	2	.	.	.
cord-253024-b393ea2u	237	1	Cultures	culture	NNS
cord-253024-b393ea2u	237	2	of	of	IN
cord-253024-b393ea2u	237	3	cells	cell	NNS
cord-253024-b393ea2u	237	4	were	be	VBD
cord-253024-b393ea2u	237	5	infected	infect	VBN
cord-253024-b393ea2u	237	6	with	with	IN
cord-253024-b393ea2u	237	7	various	various	JJ
cord-253024-b393ea2u	237	8	RNA	RNA	NNP
cord-253024-b393ea2u	237	9	recombinant	recombinant	JJ
cord-253024-b393ea2u	237	10	viruses	virus	NNS
cord-253024-b393ea2u	237	11	and	and	CC
cord-253024-b393ea2u	237	12	intercellular	intercellular	JJ
cord-253024-b393ea2u	237	13	RNA	RNA	NNP
cord-253024-b393ea2u	237	14	was	be	VBD
cord-253024-b393ea2u	237	15	harvested	harvest	VBN
cord-253024-b393ea2u	237	16	at	at	IN
cord-253024-b393ea2u	237	17	8	8	CD
cord-253024-b393ea2u	237	18	-	-	SYM
cord-253024-b393ea2u	237	19	12	12	CD
cord-253024-b393ea2u	237	20	hr	hr	NN
cord-253024-b393ea2u	237	21	postinfection	postinfection	NN
cord-253024-b393ea2u	237	22	.	.	.
cord-253024-b393ea2u	238	1	Following	follow	VBG
cord-253024-b393ea2u	238	2	cDNA	cdna	NN
cord-253024-b393ea2u	238	3	synthesis	synthesis	NN
cord-253024-b393ea2u	238	4	and	and	CC
cord-253024-b393ea2u	238	5	30	30	CD
cord-253024-b393ea2u	238	6	rounds	round	NNS
cord-253024-b393ea2u	238	7	of	of	IN
cord-253024-b393ea2u	238	8	amplification	amplification	NN
cord-253024-b393ea2u	238	9	with	with	IN
cord-253024-b393ea2u	238	10	the	the	DT
cord-253024-b393ea2u	238	11	TAQ	taq	NN
cord-253024-b393ea2u	238	12	polymerase	polymerase	NN
cord-253024-b393ea2u	238	13	and	and	CC
cord-253024-b393ea2u	238	14	specific	specific	JJ
cord-253024-b393ea2u	238	15	oligonucleotide	oligonucleotide	JJ
cord-253024-b393ea2u	238	16	primer	primer	NN
cord-253024-b393ea2u	238	17	pairs	pair	NNS
cord-253024-b393ea2u	238	18	,	,	,
cord-253024-b393ea2u	238	19	the	the	DT
cord-253024-b393ea2u	238	20	DNA	dna	NN
cord-253024-b393ea2u	238	21	products	product	NNS
cord-253024-b393ea2u	238	22	were	be	VBD
cord-253024-b393ea2u	238	23	separated	separate	VBN
cord-253024-b393ea2u	238	24	on	on	IN
cord-253024-b393ea2u	238	25	1	1	CD
cord-253024-b393ea2u	238	26	%	%	NN
cord-253024-b393ea2u	238	27	agarose	agarose	JJ
cord-253024-b393ea2u	238	28	gels	gel	NNS
cord-253024-b393ea2u	238	29	and	and	CC
cord-253024-b393ea2u	238	30	visualized	visualize	VBN
cord-253024-b393ea2u	238	31	by	by	IN
cord-253024-b393ea2u	238	32	UV	uv	NN
cord-253024-b393ea2u	238	33	light	light	NN
cord-253024-b393ea2u	238	34	or	or	CC
cord-253024-b393ea2u	238	35	southern	southern	JJ
cord-253024-b393ea2u	238	36	blotting	blotting	NN
cord-253024-b393ea2u	238	37	techniques	technique	NNS
cord-253024-b393ea2u	238	38	(	(	-LRB-
cord-253024-b393ea2u	238	39	Fig	Fig	NNP
cord-253024-b393ea2u	238	40	.	.	.
cord-253024-b393ea2u	238	41	6	6	CD
cord-253024-b393ea2u	238	42	)	)	-RRB-
cord-253024-b393ea2u	238	43	.	.	.
cord-253024-b393ea2u	239	1	In	in	IN
cord-253024-b393ea2u	239	2	Fig	Fig	NNP
cord-253024-b393ea2u	239	3	.	.	.
cord-253024-b393ea2u	240	1	6a	6a	LS
cord-253024-b393ea2u	240	2	,	,	,
cord-253024-b393ea2u	240	3	PCR	PCR	NNP
cord-253024-b393ea2u	240	4	products	product	NNS
cord-253024-b393ea2u	240	5	spanning	span	VBG
cord-253024-b393ea2u	240	6	the	the	DT
cord-253024-b393ea2u	240	7	hypervariable	hypervariable	JJ
cord-253024-b393ea2u	240	8	region	region	NN
cord-253024-b393ea2u	240	9	of	of	IN
cord-253024-b393ea2u	240	10	MHV	MHV	NNP
cord-253024-b393ea2u	240	11	-	-	HYPH
cord-253024-b393ea2u	240	12	A59	A59	NNP
cord-253024-b393ea2u	240	13	,	,	,
cord-253024-b393ea2u	240	14	JHM	JHM	NNP
cord-253024-b393ea2u	240	15	,	,	,
cord-253024-b393ea2u	240	16	and	and	CC
cord-253024-b393ea2u	240	17	MHV2	MHV2	NNP
cord-253024-b393ea2u	240	18	clearly	clearly	RB
cord-253024-b393ea2u	240	19	demonstrate	demonstrate	VBP
cord-253024-b393ea2u	240	20	the	the	DT
cord-253024-b393ea2u	240	21	89	89	CD
cord-253024-b393ea2u	240	22	amino	amino	NN
cord-253024-b393ea2u	240	23	acid	acid	NN
cord-253024-b393ea2u	240	24	deletion	deletion	NN
cord-253024-b393ea2u	240	25	that	that	WDT
cord-253024-b393ea2u	240	26	is	be	VBZ
cord-253024-b393ea2u	240	27	present	present	JJ
cord-253024-b393ea2u	240	28	in	in	IN
cord-253024-b393ea2u	240	29	the	the	DT
cord-253024-b393ea2u	240	30	JHM	JHM	NNP
cord-253024-b393ea2u	240	31	S	S	NNP
cord-253024-b393ea2u	240	32	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	240	33	gene	gene	NN
cord-253024-b393ea2u	240	34	(	(	-LRB-
cord-253024-b393ea2u	240	35	Banner	Banner	NNP
cord-253024-b393ea2u	240	36	eta/.	eta/.	CD
cord-253024-b393ea2u	240	37	,	,	,
cord-253024-b393ea2u	240	38	1990	1990	CD
cord-253024-b393ea2u	240	39	;	;	:
cord-253024-b393ea2u	240	40	Parker	Parker	NNP
cord-253024-b393ea2u	240	41	eta/.	eta/.	NNP
cord-253024-b393ea2u	240	42	,	,	,
cord-253024-b393ea2u	240	43	1990	1990	CD
cord-253024-b393ea2u	240	44	)	)	-RRB-
cord-253024-b393ea2u	240	45	.	.	.
cord-253024-b393ea2u	241	1	Southern	southern	JJ
cord-253024-b393ea2u	241	2	blotting	blotting	NN
cord-253024-b393ea2u	241	3	techniques	technique	NNS
cord-253024-b393ea2u	241	4	using	use	VBG
cord-253024-b393ea2u	241	5	an	an	DT
cord-253024-b393ea2u	241	6	internal	internal	JJ
cord-253024-b393ea2u	241	7	oligomer	oligomer	NNP
cord-253024-b393ea2u	241	8	probe	probe	NN
cord-253024-b393ea2u	241	9	demonstrate	demonstrate	VB
cord-253024-b393ea2u	241	10	that	that	IN
cord-253024-b393ea2u	241	11	the	the	DT
cord-253024-b393ea2u	241	12	-1.5-kb	-1.5-kb	CD
cord-253024-b393ea2u	241	13	fragment	fragment	NN
cord-253024-b393ea2u	241	14	is	be	VBZ
cord-253024-b393ea2u	241	15	specific	specific	JJ
cord-253024-b393ea2u	241	16	for	for	IN
cord-253024-b393ea2u	241	17	the	the	DT
cord-253024-b393ea2u	241	18	MHV	MHV	NNP
cord-253024-b393ea2u	241	19	S	S	NNP
cord-253024-b393ea2u	241	20	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	241	21	sequences	sequence	NNS
cord-253024-b393ea2u	241	22	spanning	span	VBG
cord-253024-b393ea2u	241	23	nucleotides	nucleotide	NNS
cord-253024-b393ea2u	241	24	642	642	CD
cord-253024-b393ea2u	241	25	-	-	SYM
cord-253024-b393ea2u	241	26	21	21	CD
cord-253024-b393ea2u	241	27	15	15	CD
cord-253024-b393ea2u	241	28	and	and	CC
cord-253024-b393ea2u	241	29	is	be	VBZ
cord-253024-b393ea2u	241	30	not	not	RB
cord-253024-b393ea2u	241	31	present	present	JJ
cord-253024-b393ea2u	241	32	in	in	IN
cord-253024-b393ea2u	241	33	uninfected	uninfected	JJ
cord-253024-b393ea2u	241	34	cells	cell	NNS
cord-253024-b393ea2u	241	35	(	(	-LRB-
cord-253024-b393ea2u	242	1	Fig	Fig	NNP
cord-253024-b393ea2u	242	2	.	.	.
cord-253024-b393ea2u	242	3	 	 	_SP
cord-253024-b393ea2u	242	4	6b	6b	CD
cord-253024-b393ea2u	242	5	)	)	-RRB-
cord-253024-b393ea2u	242	6	.	.	.
cord-253024-b393ea2u	243	1	No	no	DT
cord-253024-b393ea2u	243	2	obvious	obvious	JJ
cord-253024-b393ea2u	243	3	deletions	deletion	NNS
cord-253024-b393ea2u	243	4	were	be	VBD
cord-253024-b393ea2u	243	5	detected	detect	VBN
cord-253024-b393ea2u	243	6	through	through	IN
cord-253024-b393ea2u	243	7	the	the	DT
cord-253024-b393ea2u	243	8	hypervariable	hypervariable	JJ
cord-253024-b393ea2u	243	9	region	region	NN
cord-253024-b393ea2u	243	10	(	(	-LRB-
cord-253024-b393ea2u	243	11	Fig	Fig	NNP
cord-253024-b393ea2u	243	12	.	.	.
cord-253024-b393ea2u	243	13	6c	6c	NNP
cord-253024-b393ea2u	243	14	)	)	-RRB-
cord-253024-b393ea2u	243	15	,	,	,
cord-253024-b393ea2u	243	16	N	n	CD
cord-253024-b393ea2u	243	17	-	-	HYPH
cord-253024-b393ea2u	243	18	terminus	terminus	NN
cord-253024-b393ea2u	243	19	,	,	,
cord-253024-b393ea2u	243	20	or	or	CC
cord-253024-b393ea2u	243	21	C	C	NNP
cord-253024-b393ea2u	243	22	terminus	terminus	NN
cord-253024-b393ea2u	243	23	in	in	IN
cord-253024-b393ea2u	243	24	the	the	DT
cord-253024-b393ea2u	243	25	S	S	NNP
cord-253024-b393ea2u	243	26	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	243	27	gene	gene	NN
cord-253024-b393ea2u	243	28	of	of	IN
cord-253024-b393ea2u	243	29	over	over	IN
cord-253024-b393ea2u	243	30	20	20	CD
cord-253024-b393ea2u	243	31	different	different	JJ
cord-253024-b393ea2u	243	32	RNA	RNA	NNP
cord-253024-b393ea2u	243	33	recombinant	recombinant	JJ
cord-253024-b393ea2u	243	34	viruses	virus	NNS
cord-253024-b393ea2u	243	35	tested	test	VBN
cord-253024-b393ea2u	243	36	(	(	-LRB-
cord-253024-b393ea2u	243	37	Fig	Fig	NNP
cord-253024-b393ea2u	243	38	.	.	.
cord-253024-b393ea2u	243	39	6d	6d	NNP
cord-253024-b393ea2u	243	40	)	)	-RRB-
cord-253024-b393ea2u	243	41	.	.	.
cord-253024-b393ea2u	244	1	Since	since	IN
cord-253024-b393ea2u	244	2	the	the	DT
cord-253024-b393ea2u	244	3	distance	distance	NN
cord-253024-b393ea2u	244	4	between	between	IN
cord-253024-b393ea2u	244	5	the	the	DT
cord-253024-b393ea2u	244	6	group	group	NN
cord-253024-b393ea2u	244	7	Fl	Fl	NNP
cord-253024-b393ea2u	244	8	and	and	CC
cord-253024-b393ea2u	244	9	F2	f2	NN
cord-253024-b393ea2u	244	10	mutants	mutant	NNS
cord-253024-b393ea2u	244	11	would	would	MD
cord-253024-b393ea2u	244	12	have	have	VB
cord-253024-b393ea2u	244	13	required	require	VBN
cord-253024-b393ea2u	244	14	sizable	sizable	JJ
cord-253024-b393ea2u	244	15	deletions	deletion	NNS
cord-253024-b393ea2u	244	16	(	(	-LRB-
cord-253024-b393ea2u	244	17	>	>	XX
cord-253024-b393ea2u	244	18	lOO	loo	ADD
cord-253024-b393ea2u	244	19	bp	bp	NNP
cord-253024-b393ea2u	244	20	)	)	-RRB-
cord-253024-b393ea2u	244	21	to	to	TO
cord-253024-b393ea2u	244	22	result	result	VB
cord-253024-b393ea2u	244	23	in	in	IN
cord-253024-b393ea2u	244	24	ts+	ts+	NN
cord-253024-b393ea2u	244	25	virus	virus	NN
cord-253024-b393ea2u	244	26	(	(	-LRB-
cord-253024-b393ea2u	244	27	Fig	Fig	NNP
cord-253024-b393ea2u	244	28	.	.	.
cord-253024-b393ea2u	244	29	4	4	LS
cord-253024-b393ea2u	244	30	)	)	-RRB-
cord-253024-b393ea2u	244	31	these	these	DT
cord-253024-b393ea2u	244	32	data	datum	NNS
cord-253024-b393ea2u	244	33	suggested	suggest	VBD
cord-253024-b393ea2u	244	34	that	that	IN
cord-253024-b393ea2u	244	35	large	large	JJ
cord-253024-b393ea2u	244	36	deletions	deletion	NNS
cord-253024-b393ea2u	244	37	were	be	VBD
cord-253024-b393ea2u	244	38	rare	rare	JJ
cord-253024-b393ea2u	244	39	events	event	NNS
cord-253024-b393ea2u	244	40	in	in	IN
cord-253024-b393ea2u	244	41	these	these	DT
cord-253024-b393ea2u	244	42	crosses	crosse	NNS
cord-253024-b393ea2u	244	43	,	,	,
cord-253024-b393ea2u	244	44	and	and	CC
cord-253024-b393ea2u	244	45	did	do	VBD
cord-253024-b393ea2u	244	46	not	not	RB
cord-253024-b393ea2u	244	47	contribute	contribute	VB
cord-253024-b393ea2u	244	48	to	to	IN
cord-253024-b393ea2u	244	49	an	an	DT
cord-253024-b393ea2u	244	50	increase	increase	NN
cord-253024-b393ea2u	244	51	in	in	IN
cord-253024-b393ea2u	244	52	the	the	DT
cord-253024-b393ea2u	244	53	recombination	recombination	NN
cord-253024-b393ea2u	244	54	frequency	frequency	NN
cord-253024-b393ea2u	244	55	within	within	IN
cord-253024-b393ea2u	244	56	the	the	DT
cord-253024-b393ea2u	244	57	S	S	NNP
cord-253024-b393ea2u	244	58	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	244	59	gene	gene	NN
cord-253024-b393ea2u	244	60	.	.	.
cord-253024-b393ea2u	245	1	These	these	DT
cord-253024-b393ea2u	245	2	studies	study	NNS
cord-253024-b393ea2u	245	3	can	can	MD
cord-253024-b393ea2u	245	4	not	not	RB
cord-253024-b393ea2u	245	5	rule	rule	VB
cord-253024-b393ea2u	245	6	out	out	RP
cord-253024-b393ea2u	245	7	the	the	DT
cord-253024-b393ea2u	245	8	possibility	possibility	NN
cord-253024-b393ea2u	245	9	of	of	IN
cord-253024-b393ea2u	245	10	small	small	JJ
cord-253024-b393ea2u	245	11	deletions	deletion	NNS
cord-253024-b393ea2u	245	12	in	in	IN
cord-253024-b393ea2u	245	13	one	one	CD
cord-253024-b393ea2u	245	14	ts	ts	JJ
cord-253024-b393ea2u	245	15	parent	parent	NN
cord-253024-b393ea2u	245	16	contributing	contribute	VBG
cord-253024-b393ea2u	245	17	to	to	IN
cord-253024-b393ea2u	245	18	an	an	DT
cord-253024-b393ea2u	245	19	increase	increase	NN
cord-253024-b393ea2u	245	20	in	in	IN
cord-253024-b393ea2u	245	21	the	the	DT
cord-253024-b393ea2u	245	22	ts+	ts+	XX
cord-253024-b393ea2u	245	23	progeny	progeny	VB
cord-253024-b393ea2u	245	24	.	.	.
cord-253024-b393ea2u	246	1	However	however	RB
cord-253024-b393ea2u	246	2	,	,	,
cord-253024-b393ea2u	246	3	no	no	DT
cord-253024-b393ea2u	246	4	obvious	obvious	JJ
cord-253024-b393ea2u	246	5	deletions	deletion	NNS
cord-253024-b393ea2u	246	6	were	be	VBD
cord-253024-b393ea2u	246	7	detected	detect	VBN
cord-253024-b393ea2u	246	8	in	in	IN
cord-253024-b393ea2u	246	9	the	the	DT
cord-253024-b393ea2u	246	10	S	S	NNP
cord-253024-b393ea2u	246	11	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	246	12	gene	gene	NN
cord-253024-b393ea2u	246	13	of	of	IN
cord-253024-b393ea2u	246	14	19	19	CD
cord-253024-b393ea2u	246	15	different	different	JJ
cord-253024-b393ea2u	246	16	revertant	revertant	NN
cord-253024-b393ea2u	246	17	viruses	virus	NNS
cord-253024-b393ea2u	246	18	.	.	.
cord-253024-b393ea2u	247	1	In	in	IN
cord-253024-b393ea2u	247	2	Fig	Fig	NNP
cord-253024-b393ea2u	247	3	.	.	.
cord-253024-b393ea2u	248	1	6e	6e	NNP
cord-253024-b393ea2u	248	2	,	,	,
cord-253024-b393ea2u	248	3	PCR	PCR	NNP
cord-253024-b393ea2u	248	4	products	product	NNS
cord-253024-b393ea2u	248	5	spanning	span	VBG
cord-253024-b393ea2u	248	6	the	the	DT
cord-253024-b393ea2u	248	7	hypervariable	hypervariable	JJ
cord-253024-b393ea2u	248	8	region	region	NN
cord-253024-b393ea2u	248	9	of	of	IN
cord-253024-b393ea2u	248	10	NC17	NC17	NNP
cord-253024-b393ea2u	248	11	and	and	CC
cord-253024-b393ea2u	248	12	its	-PRON-	PRP$
cord-253024-b393ea2u	248	13	four	four	CD
cord-253024-b393ea2u	248	14	revertants	revertant	NNS
cord-253024-b393ea2u	248	15	are	be	VBP
cord-253024-b393ea2u	248	16	presented	present	VBN
cord-253024-b393ea2u	248	17	.	.	.
cord-253024-b393ea2u	249	1	While	while	IN
cord-253024-b393ea2u	249	2	these	these	DT
cord-253024-b393ea2u	249	3	data	datum	NNS
cord-253024-b393ea2u	249	4	can	can	MD
cord-253024-b393ea2u	249	5	not	not	RB
cord-253024-b393ea2u	249	6	conclusively	conclusively	RB
cord-253024-b393ea2u	249	7	rule	rule	VB
cord-253024-b393ea2u	249	8	out	out	RP
cord-253024-b393ea2u	249	9	the	the	DT
cord-253024-b393ea2u	249	10	possibility	possibility	NN
cord-253024-b393ea2u	249	11	of	of	IN
cord-253024-b393ea2u	249	12	small	small	JJ
cord-253024-b393ea2u	249	13	deletions	deletion	NNS
cord-253024-b393ea2u	249	14	(	(	-LRB-
cord-253024-b393ea2u	249	15	~50	~50	NFP
cord-253024-b393ea2u	249	16	bp	bp	NNP
cord-253024-b393ea2u	249	17	)	)	-RRB-
cord-253024-b393ea2u	249	18	that	that	WDT
cord-253024-b393ea2u	249	19	would	would	MD
cord-253024-b393ea2u	249	20	not	not	RB
cord-253024-b393ea2u	249	21	be	be	VB
cord-253024-b393ea2u	249	22	detected	detect	VBN
cord-253024-b393ea2u	249	23	under	under	IN
cord-253024-b393ea2u	249	24	these	these	DT
cord-253024-b393ea2u	249	25	conditions	condition	NNS
cord-253024-b393ea2u	249	26	,	,	,
cord-253024-b393ea2u	249	27	the	the	DT
cord-253024-b393ea2u	249	28	data	datum	NNS
cord-253024-b393ea2u	249	29	suggested	suggest	VBD
cord-253024-b393ea2u	249	30	that	that	IN
cord-253024-b393ea2u	249	31	most	most	JJS
cord-253024-b393ea2u	249	32	revertants	revertant	NNS
cord-253024-b393ea2u	249	33	probably	probably	RB
cord-253024-b393ea2u	249	34	contain	contain	VBP
cord-253024-b393ea2u	249	35	a	a	DT
cord-253024-b393ea2u	249	36	single	single	JJ
cord-253024-b393ea2u	249	37	nucleotide	nucleotide	JJ
cord-253024-b393ea2u	249	38	reversion	reversion	NN
cord-253024-b393ea2u	249	39	.	.	.
cord-253024-b393ea2u	250	1	Although	although	IN
cord-253024-b393ea2u	250	2	homologous	homologous	JJ
cord-253024-b393ea2u	250	3	recombination	recombination	NN
cord-253024-b393ea2u	250	4	occurs	occur	VBZ
cord-253024-b393ea2u	250	5	during	during	IN
cord-253024-b393ea2u	250	6	the	the	DT
cord-253024-b393ea2u	250	7	replication	replication	NN
cord-253024-b393ea2u	250	8	strategy	strategy	NN
cord-253024-b393ea2u	250	9	of	of	IN
cord-253024-b393ea2u	250	10	several	several	JJ
cord-253024-b393ea2u	250	11	nonsegmented	nonsegmente	VBN
cord-253024-b393ea2u	250	12	RNA	RNA	NNP
cord-253024-b393ea2u	250	13	viruses	virus	NNS
cord-253024-b393ea2u	250	14	(	(	-LRB-
cord-253024-b393ea2u	250	15	Bujarski	Bujarski	NNP
cord-253024-b393ea2u	250	16	and	and	CC
cord-253024-b393ea2u	250	17	Kaesberg	Kaesberg	NNP
cord-253024-b393ea2u	250	18	,	,	,
cord-253024-b393ea2u	250	19	1986	1986	CD
cord-253024-b393ea2u	250	20	;	;	:
cord-253024-b393ea2u	250	21	Cooper	Cooper	NNP
cord-253024-b393ea2u	250	22	,	,	,
cord-253024-b393ea2u	250	23	1968	1968	CD
cord-253024-b393ea2u	250	24	;	;	:
cord-253024-b393ea2u	250	25	Hahn	Hahn	NNP
cord-253024-b393ea2u	250	26	et	et	NNP
cord-253024-b393ea2u	250	27	al	al	NNP
cord-253024-b393ea2u	250	28	.	.	NNP
cord-253024-b393ea2u	250	29	,	,	,
cord-253024-b393ea2u	250	30	1988	1988	CD
cord-253024-b393ea2u	250	31	;	;	:
cord-253024-b393ea2u	250	32	King	King	NNP
cord-253024-b393ea2u	250	33	el	el	NNP
cord-253024-b393ea2u	250	34	al	al	NNP
cord-253024-b393ea2u	250	35	.	.	NNP
cord-253024-b393ea2u	250	36	,	,	,
cord-253024-b393ea2u	250	37	1987	1987	CD
cord-253024-b393ea2u	250	38	;	;	:
cord-253024-b393ea2u	250	39	Weiss	Weiss	NNP
cord-253024-b393ea2u	250	40	and	and	CC
cord-253024-b393ea2u	250	41	Schlesinger	Schlesinger	NNP
cord-253024-b393ea2u	250	42	,	,	,
cord-253024-b393ea2u	250	43	1991	1991	CD
cord-253024-b393ea2u	250	44	)	)	-RRB-
cord-253024-b393ea2u	250	45	,	,	,
cord-253024-b393ea2u	250	46	high	high	JJ
cord-253024-b393ea2u	250	47	frequency	frequency	NN
cord-253024-b393ea2u	250	48	RNA	RNA	NNP
cord-253024-b393ea2u	250	49	recombination	recombination	NN
cord-253024-b393ea2u	250	50	is	be	VBZ
cord-253024-b393ea2u	250	51	probably	probably	RB
cord-253024-b393ea2u	250	52	a	a	DT
cord-253024-b393ea2u	250	53	unique	unique	JJ
cord-253024-b393ea2u	250	54	phenomenon	phenomenon	NN
cord-253024-b393ea2u	250	55	associated	associate	VBN
cord-253024-b393ea2u	250	56	with	with	IN
cord-253024-b393ea2u	250	57	MHV	MHV	NNP
cord-253024-b393ea2u	250	58	and	and	CC
cord-253024-b393ea2u	250	59	perhaps	perhaps	RB
cord-253024-b393ea2u	250	60	the	the	DT
cord-253024-b393ea2u	250	61	replication	replication	NN
cord-253024-b393ea2u	250	62	strategies	strategy	NNS
cord-253024-b393ea2u	250	63	of	of	IN
cord-253024-b393ea2u	250	64	other	other	JJ
cord-253024-b393ea2u	250	65	coronavirus	coronavirus	NN
cord-253024-b393ea2u	250	66	Keck	Keck	NNP
cord-253024-b393ea2u	250	67	et	et	FW
cord-253024-b393ea2u	250	68	al	al	NNP
cord-253024-b393ea2u	250	69	.	.	NNP
cord-253024-b393ea2u	250	70	,	,	,
cord-253024-b393ea2u	250	71	1987	1987	CD
cord-253024-b393ea2u	251	1	Keck	Keck	NNP
cord-253024-b393ea2u	251	2	et	et	NNP
cord-253024-b393ea2u	251	3	al	al	NNP
cord-253024-b393ea2u	251	4	.	.	.
cord-253024-b393ea2u	252	1	,	,	,
cord-253024-b393ea2u	253	1	,	,	,
cord-253024-b393ea2u	253	2	1988a	1988a	CD
cord-253024-b393ea2u	253	3	Makino	Makino	NNP
cord-253024-b393ea2u	253	4	et	et	NNP
cord-253024-b393ea2u	253	5	a/.	a/.	NN
cord-253024-b393ea2u	253	6	,	,	,
cord-253024-b393ea2u	253	7	1986b	1986b	CD
cord-253024-b393ea2u	253	8	Makino	Makino	NNP
cord-253024-b393ea2u	253	9	et	et	NNP
cord-253024-b393ea2u	253	10	a/.	a/.	NN
cord-253024-b393ea2u	253	11	,	,	,
cord-253024-b393ea2u	253	12	,	,	,
cord-253024-b393ea2u	253	13	1987	1987	CD
cord-253024-b393ea2u	253	14	Lai	Lai	NNP
cord-253024-b393ea2u	253	15	et	et	NNP
cord-253024-b393ea2u	253	16	a/.	a/.	NN
cord-253024-b393ea2u	253	17	,	,	,
cord-253024-b393ea2u	253	18	1985	1985	CD
cord-253024-b393ea2u	253	19	)	)	-RRB-
cord-253024-b393ea2u	253	20	.	.	.
cord-253024-b393ea2u	254	1	Using	use	VBG
cord-253024-b393ea2u	254	2	highly	highly	RB
cord-253024-b393ea2u	254	3	characterized	characterize	VBN
cord-253024-b393ea2u	254	4	ts	ts	NN
cord-253024-b393ea2u	254	5	mutants	mutant	NNS
cord-253024-b393ea2u	254	6	of	of	IN
cord-253024-b393ea2u	254	7	MHV	MHV	NNP
cord-253024-b393ea2u	254	8	-	-	HYPH
cord-253024-b393ea2u	254	9	A59	A59	NNP
cord-253024-b393ea2u	254	10	,	,	,
cord-253024-b393ea2u	254	11	we	-PRON-	PRP
cord-253024-b393ea2u	254	12	established	establish	VBD
cord-253024-b393ea2u	254	13	a	a	DT
cord-253024-b393ea2u	254	14	genetic	genetic	JJ
cord-253024-b393ea2u	254	15	recombination	recombination	NN
cord-253024-b393ea2u	254	16	map	map	NN
cord-253024-b393ea2u	254	17	in	in	IN
cord-253024-b393ea2u	254	18	the	the	DT
cord-253024-b393ea2u	254	19	polymer-	polymer-	JJ
cord-253024-b393ea2u	254	20	Predrcted	Predrcted	NNP
cord-253024-b393ea2u	254	21	nucleotide	nucleotide	JJ
cord-253024-b393ea2u	254	22	domarns	domarn	NNS
cord-253024-b393ea2u	254	23	of	of	IN
cord-253024-b393ea2u	254	24	the	the	DT
cord-253024-b393ea2u	254	25	group	group	NN
cord-253024-b393ea2u	254	26	F	F	NNP
cord-253024-b393ea2u	254	27	RNA	RNA	NNP
cord-253024-b393ea2u	254	28	'	'	POS
cord-253024-b393ea2u	254	29	mutants	mutant	NNS
cord-253024-b393ea2u	254	30	.	.	.
cord-253024-b393ea2u	255	1	Assumrng	Assumrng	NNP
cord-253024-b393ea2u	255	2	a	a	DT
cord-253024-b393ea2u	255	3	recombination	recombination	NN
cord-253024-b393ea2u	255	4	frequency	frequency	NN
cord-253024-b393ea2u	255	5	of	of	IN
cord-253024-b393ea2u	255	6	lo/01575	lo/01575	NNP
cord-253024-b393ea2u	255	7	bp	bp	NNP
cord-253024-b393ea2u	255	8	of	of	IN
cord-253024-b393ea2u	255	9	dsRNA	dsRNA	NNP
cord-253024-b393ea2u	255	10	and	and	CC
cord-253024-b393ea2u	255	11	that	that	IN
cord-253024-b393ea2u	255	12	LA7	LA7	NNP
cord-253024-b393ea2u	255	13	maps	map	VBZ
cord-253024-b393ea2u	255	14	roughly	roughly	RB
cord-253024-b393ea2u	255	15	0.5	0.5	CD
cord-253024-b393ea2u	255	16	kb	kb	NNS
cord-253024-b393ea2u	255	17	from	from	IN
cord-253024-b393ea2u	255	18	the	the	DT
cord-253024-b393ea2u	255	19	5	5	CD
cord-253024-b393ea2u	255	20	'	'	NN
cord-253024-b393ea2u	255	21	end	end	NN
cord-253024-b393ea2u	255	22	of	of	IN
cord-253024-b393ea2u	255	23	the	the	DT
cord-253024-b393ea2u	255	24	S	S	NNP
cord-253024-b393ea2u	255	25	glycoprotern	glycoprotern	NNP
cord-253024-b393ea2u	255	26	gene	gene	NN
cord-253024-b393ea2u	255	27	,	,	,
cord-253024-b393ea2u	256	1	the	the	DT
cord-253024-b393ea2u	256	2	tentatrve	tentatrve	NNP
cord-253024-b393ea2u	256	3	nucleotide	nucleotide	JJ
cord-253024-b393ea2u	256	4	domains	domain	NNS
cord-253024-b393ea2u	256	5	of	of	IN
cord-253024-b393ea2u	256	6	the	the	DT
cord-253024-b393ea2u	256	7	mutants	mutant	NNS
cord-253024-b393ea2u	256	8	mapping	mapping	NN
cord-253024-b393ea2u	256	9	in	in	IN
cord-253024-b393ea2u	256	10	the	the	DT
cord-253024-b393ea2u	256	11	Fl	Fl	NNP
cord-253024-b393ea2u	256	12	and	and	CC
cord-253024-b393ea2u	256	13	F2	F2	NNP
cord-253024-b393ea2u	256	14	subgroups	subgroup	NNS
cord-253024-b393ea2u	256	15	were	be	VBD
cord-253024-b393ea2u	256	16	predrcted	predrcte	VBN
cord-253024-b393ea2u	256	17	.	.	.
cord-253024-b393ea2u	257	1	The	the	DT
cord-253024-b393ea2u	257	2	hatched	hatch	VBN
cord-253024-b393ea2u	257	3	boxes	box	NNS
cord-253024-b393ea2u	257	4	represent	represent	VBP
cord-253024-b393ea2u	257	5	the	the	DT
cord-253024-b393ea2u	257	6	locatron	locatron	NN
cord-253024-b393ea2u	257	7	of	of	IN
cord-253024-b393ea2u	257	8	the	the	DT
cord-253024-b393ea2u	257	9	region	region	NN
cord-253024-b393ea2u	257	10	of	of	IN
cord-253024-b393ea2u	257	11	polymorphism	polymorphism	NN
cord-253024-b393ea2u	257	12	and	and	CC
cord-253024-b393ea2u	257	13	putative	putative	JJ
cord-253024-b393ea2u	257	14	hot	hot	JJ
cord-253024-b393ea2u	257	15	spot	spot	NN
cord-253024-b393ea2u	257	16	of	of	IN
cord-253024-b393ea2u	257	17	recombination	recombination	NN
cord-253024-b393ea2u	257	18	in	in	IN
cord-253024-b393ea2u	257	19	the	the	DT
cord-253024-b393ea2u	257	20	MHV	MHV	NNP
cord-253024-b393ea2u	257	21	genome	genome	NN
cord-253024-b393ea2u	258	1	(	(	-LRB-
cord-253024-b393ea2u	258	2	Banner	Banner	NNP
cord-253024-b393ea2u	258	3	et	et	NNP
cord-253024-b393ea2u	258	4	a/.	a/.	NNP
cord-253024-b393ea2u	258	5	,	,	,
cord-253024-b393ea2u	258	6	1990	1990	CD
cord-253024-b393ea2u	258	7	;	;	:
cord-253024-b393ea2u	258	8	Parker	Parker	NNP
cord-253024-b393ea2u	258	9	et	et	NNP
cord-253024-b393ea2u	258	10	al	al	NNP
cord-253024-b393ea2u	258	11	.	.	NNP
cord-253024-b393ea2u	258	12	,	,	,
cord-253024-b393ea2u	258	13	1989	1989	CD
cord-253024-b393ea2u	258	14	)	)	-RRB-
cord-253024-b393ea2u	258	15	.	.	.
cord-253024-b393ea2u	259	1	Arrows	arrow	NNS
cord-253024-b393ea2u	259	2	represent	represent	VBP
cord-253024-b393ea2u	259	3	the	the	DT
cord-253024-b393ea2u	259	4	approxrmate	approxrmate	NNP
cord-253024-b393ea2u	259	5	locatron	locatron	NNP
cord-253024-b393ea2u	259	6	of	of	IN
cord-253024-b393ea2u	259	7	neutralizing	neutralize	VBG
cord-253024-b393ea2u	259	8	epitopes	epitope	NNS
cord-253024-b393ea2u	259	9	In	in	IN
cord-253024-b393ea2u	259	10	the	the	DT
cord-253024-b393ea2u	259	11	S	S	NNP
cord-253024-b393ea2u	259	12	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	259	13	(	(	-LRB-
cord-253024-b393ea2u	259	14	Routledge	Routledge	NNP
cord-253024-b393ea2u	259	15	et	et	NNP
cord-253024-b393ea2u	259	16	al	al	NNP
cord-253024-b393ea2u	259	17	.	.	NNP
cord-253024-b393ea2u	259	18	,	,	,
cord-253024-b393ea2u	259	19	1991	1991	CD
cord-253024-b393ea2u	259	20	;	;	:
cord-253024-b393ea2u	260	1	Weissmiller	Weissmiller	NNP
cord-253024-b393ea2u	260	2	et	et	NNP
cord-253024-b393ea2u	260	3	a/.	a/.	NNP
cord-253024-b393ea2u	260	4	,	,	,
cord-253024-b393ea2u	260	5	1990	1990	CD
cord-253024-b393ea2u	260	6	)	)	-RRB-
cord-253024-b393ea2u	260	7	.	.	.
cord-253024-b393ea2u	261	1	To	to	TO
cord-253024-b393ea2u	261	2	determine	determine	VB
cord-253024-b393ea2u	261	3	if	if	IN
cord-253024-b393ea2u	261	4	recombinatron	recombinatron	NN
cord-253024-b393ea2u	261	5	events	event	NNS
cord-253024-b393ea2u	261	6	resulting	result	VBG
cord-253024-b393ea2u	261	7	in	in	IN
cord-253024-b393ea2u	261	8	deletions	deletion	NNS
cord-253024-b393ea2u	261	9	were	be	VBD
cord-253024-b393ea2u	261	10	contributing	contribute	VBG
cord-253024-b393ea2u	261	11	to	to	IN
cord-253024-b393ea2u	261	12	the	the	DT
cord-253024-b393ea2u	261	13	increased	increase	VBN
cord-253024-b393ea2u	261	14	recombinatron	recombinatron	NN
cord-253024-b393ea2u	261	15	rate	rate	NN
cord-253024-b393ea2u	261	16	in	in	IN
cord-253024-b393ea2u	261	17	the	the	DT
cord-253024-b393ea2u	261	18	S	S	NNP
cord-253024-b393ea2u	261	19	glycoprotern	glycoprotern	NNP
cord-253024-b393ea2u	261	20	gene	gene	NN
cord-253024-b393ea2u	261	21	,	,	,
cord-253024-b393ea2u	261	22	a	a	DT
cord-253024-b393ea2u	261	23	series	series	NN
cord-253024-b393ea2u	261	24	of	of	IN
cord-253024-b393ea2u	261	25	crosses	crosse	NNS
cord-253024-b393ea2u	261	26	were	be	VBD
cord-253024-b393ea2u	261	27	obtained	obtain	VBN
cord-253024-b393ea2u	261	28	between	between	IN
cord-253024-b393ea2u	261	29	different	different	JJ
cord-253024-b393ea2u	261	30	RNA+	RNA+	NNP
cord-253024-b393ea2u	261	31	mutants	mutant	NNS
cord-253024-b393ea2u	261	32	as	as	IN
cord-253024-b393ea2u	261	33	shown	show	VBN
cord-253024-b393ea2u	261	34	at	at	IN
cord-253024-b393ea2u	261	35	the	the	DT
cord-253024-b393ea2u	261	36	top	top	NN
cord-253024-b393ea2u	261	37	of	of	IN
cord-253024-b393ea2u	261	38	the	the	DT
cord-253024-b393ea2u	261	39	figure	figure	NN
cord-253024-b393ea2u	261	40	.	.	.
cord-253024-b393ea2u	262	1	PCR	PCR	NNP
cord-253024-b393ea2u	262	2	products	product	NNS
cord-253024-b393ea2u	262	3	spannrng	spannrng	VBP
cord-253024-b393ea2u	262	4	different	different	JJ
cord-253024-b393ea2u	262	5	portions	portion	NNS
cord-253024-b393ea2u	262	6	of	of	IN
cord-253024-b393ea2u	262	7	the	the	DT
cord-253024-b393ea2u	262	8	MHV	MHV	NNP
cord-253024-b393ea2u	262	9	S	S	NNP
cord-253024-b393ea2u	262	10	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	262	11	gene	gene	NN
cord-253024-b393ea2u	262	12	were	be	VBD
cord-253024-b393ea2u	262	13	obtained	obtain	VBN
cord-253024-b393ea2u	262	14	using	use	VBG
cord-253024-b393ea2u	262	15	specific	specific	JJ
cord-253024-b393ea2u	262	16	oligomer	oligomer	NNP
cord-253024-b393ea2u	262	17	products	product	NNS
cord-253024-b393ea2u	262	18	and	and	CC
cord-253024-b393ea2u	262	19	the	the	DT
cord-253024-b393ea2u	262	20	TAQ	taq	NN
cord-253024-b393ea2u	262	21	polymerase	polymerase	NN
cord-253024-b393ea2u	262	22	.	.	.
cord-253024-b393ea2u	263	1	The	the	DT
cord-253024-b393ea2u	263	2	locatron	locatron	NN
cord-253024-b393ea2u	263	3	of	of	IN
cord-253024-b393ea2u	263	4	these	these	DT
cord-253024-b393ea2u	263	5	primer	primer	NN
cord-253024-b393ea2u	263	6	pairs	pair	NNS
cord-253024-b393ea2u	263	7	and	and	CC
cord-253024-b393ea2u	263	8	their	-PRON-	PRP$
cord-253024-b393ea2u	263	9	predicted	predict	VBN
cord-253024-b393ea2u	263	10	products	product	NNS
cord-253024-b393ea2u	263	11	are	be	VBP
cord-253024-b393ea2u	263	12	shown	show	VBN
cord-253024-b393ea2u	263	13	in	in	IN
cord-253024-b393ea2u	263	14	the	the	DT
cord-253024-b393ea2u	263	15	bottom	bottom	NN
cord-253024-b393ea2u	263	16	of	of	IN
cord-253024-b393ea2u	263	17	the	the	DT
cord-253024-b393ea2u	263	18	figure	figure	NN
cord-253024-b393ea2u	263	19	.	.	.
cord-253024-b393ea2u	264	1	To	to	TO
cord-253024-b393ea2u	264	2	predict	predict	VB
cord-253024-b393ea2u	264	3	the	the	DT
cord-253024-b393ea2u	264	4	maximum	maximum	JJ
cord-253024-b393ea2u	264	5	recombination	recombination	NN
cord-253024-b393ea2u	264	6	frequency	frequency	NN
cord-253024-b393ea2u	264	7	at	at	IN
cord-253024-b393ea2u	264	8	the	the	DT
cord-253024-b393ea2u	264	9	5'end	5'end	CD
cord-253024-b393ea2u	264	10	of	of	IN
cord-253024-b393ea2u	264	11	the	the	DT
cord-253024-b393ea2u	264	12	genome	genome	NN
cord-253024-b393ea2u	264	13	,	,	,
cord-253024-b393ea2u	264	14	we	-PRON-	PRP
cord-253024-b393ea2u	264	15	calculated	calculate	VBD
cord-253024-b393ea2u	264	16	the	the	DT
cord-253024-b393ea2u	264	17	recombination	recombination	NN
cord-253024-b393ea2u	264	18	frequency	frequency	NN
cord-253024-b393ea2u	264	19	between	between	IN
cord-253024-b393ea2u	264	20	the	the	DT
cord-253024-b393ea2u	264	21	three	three	CD
cord-253024-b393ea2u	264	22	most	most	RBS
cord-253024-b393ea2u	264	23	distant	distant	JJ
cord-253024-b393ea2u	264	24	RNA	RNA	NNP
cord-253024-b393ea2u	264	25	-	-	HYPH
cord-253024-b393ea2u	264	26	mutants	mutant	NNS
cord-253024-b393ea2u	264	27	(	(	-LRB-
cord-253024-b393ea2u	264	28	Group	Group	NNP
cord-253024-b393ea2u	264	29	A	a	DT
cord-253024-b393ea2u	264	30	RNA	RNA	NNP
cord-253024-b393ea2u	264	31	-	-	HYPH
cord-253024-b393ea2u	264	32	mutants	mutant	NNS
cord-253024-b393ea2u	264	33	:	:	:
cord-253024-b393ea2u	264	34	LA3	LA3	NNS
cord-253024-b393ea2u	264	35	.	.	.
cord-253024-b393ea2u	265	1	LA6	LA6	NNP
cord-253024-b393ea2u	265	2	;	;	:
cord-253024-b393ea2u	265	3	Group	Group	NNP
cord-253024-b393ea2u	265	4	F	F	NNP
cord-253024-b393ea2u	265	5	RNA	RNA	NNP
cord-253024-b393ea2u	265	6	-	-	HYPH
cord-253024-b393ea2u	265	7	mutant	mutant	NN
cord-253024-b393ea2u	265	8	:	:	:
cord-253024-b393ea2u	266	1	LA1	LA1	NNP
cord-253024-b393ea2u	266	2	8)	8)	NNP
cord-253024-b393ea2u	266	3	assuming	assume	VBG
cord-253024-b393ea2u	266	4	that	that	IN
cord-253024-b393ea2u	266	5	these	these	DT
cord-253024-b393ea2u	266	6	mutants	mutant	NNS
cord-253024-b393ea2u	266	7	map	map	VBP
cord-253024-b393ea2u	266	8	at	at	IN
cord-253024-b393ea2u	266	9	the	the	DT
cord-253024-b393ea2u	266	10	most	most	RBS
cord-253024-b393ea2u	266	11	distant	distant	JJ
cord-253024-b393ea2u	266	12	ends	end	NNS
cord-253024-b393ea2u	266	13	of	of	IN
cord-253024-b393ea2u	266	14	the	the	DT
cord-253024-b393ea2u	266	15	22.kb	22.kb	CD
cord-253024-b393ea2u	266	16	polymerase	polymerase	NN
cord-253024-b393ea2u	266	17	region	region	NN
cord-253024-b393ea2u	266	18	(	(	-LRB-
cord-253024-b393ea2u	266	19	-21	-21	IN
cord-253024-b393ea2u	266	20	kb	kb	NNP
cord-253024-b393ea2u	266	21	apart	apart	RB
cord-253024-b393ea2u	266	22	)	)	-RRB-
cord-253024-b393ea2u	266	23	.	.	.
cord-253024-b393ea2u	267	1	To	to	TO
cord-253024-b393ea2u	267	2	map	map	VB
cord-253024-b393ea2u	267	3	the	the	DT
cord-253024-b393ea2u	267	4	maximum	maximum	JJ
cord-253024-b393ea2u	267	5	recombination	recombination	NN
cord-253024-b393ea2u	267	6	rate	rate	NN
cord-253024-b393ea2u	267	7	across	across	IN
cord-253024-b393ea2u	267	8	the	the	DT
cord-253024-b393ea2u	267	9	HE	HE	NNP
cord-253024-b393ea2u	267	10	and	and	CC
cord-253024-b393ea2u	267	11	p30	p30	NNP
cord-253024-b393ea2u	267	12	genes	gene	NNS
cord-253024-b393ea2u	267	13	,	,	,
cord-253024-b393ea2u	267	14	recombination	recombination	NN
cord-253024-b393ea2u	267	15	rates	rate	NNS
cord-253024-b393ea2u	267	16	were	be	VBD
cord-253024-b393ea2u	267	17	determined	determine	VBN
cord-253024-b393ea2u	267	18	between	between	IN
cord-253024-b393ea2u	267	19	mutants	mutant	NNS
cord-253024-b393ea2u	267	20	mapping	map	VBG
cord-253024-b393ea2u	267	21	approximately	approximately	RB
cord-253024-b393ea2u	267	22	3.5	3.5	CD
cord-253024-b393ea2u	267	23	kb	kb	NNS
cord-253024-b393ea2u	267	24	apart	apart	RB
cord-253024-b393ea2u	267	25	at	at	IN
cord-253024-b393ea2u	267	26	the	the	DT
cord-253024-b393ea2u	267	27	3	3	CD
cord-253024-b393ea2u	267	28	'	'	NN
cord-253024-b393ea2u	267	29	end	end	NN
cord-253024-b393ea2u	267	30	of	of	IN
cord-253024-b393ea2u	267	31	the	the	DT
cord-253024-b393ea2u	267	32	polymerase	polymerase	NN
cord-253024-b393ea2u	267	33	gene	gene	NN
cord-253024-b393ea2u	267	34	(	(	-LRB-
cord-253024-b393ea2u	267	35	LA1	LA1	NNP
cord-253024-b393ea2u	267	36	8)	8)	NNP
cord-253024-b393ea2u	267	37	and	and	CC
cord-253024-b393ea2u	267	38	the	the	DT
cord-253024-b393ea2u	267	39	5	5	CD
cord-253024-b393ea2u	267	40	'	'	NN
cord-253024-b393ea2u	267	41	end	end	NN
cord-253024-b393ea2u	267	42	of	of	IN
cord-253024-b393ea2u	267	43	the	the	DT
cord-253024-b393ea2u	267	44	S	S	NNP
cord-253024-b393ea2u	267	45	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	267	46	gene	gene	NN
cord-253024-b393ea2u	267	47	(	(	-LRB-
cord-253024-b393ea2u	267	48	Group	Group	NNP
cord-253024-b393ea2u	267	49	F	F	NNP
cord-253024-b393ea2u	267	50	RNA+	RNA+	NNP
cord-253024-b393ea2u	267	51	mutants	mutant	NNS
cord-253024-b393ea2u	267	52	:	:	:
cord-253024-b393ea2u	267	53	LA7	LA7	NNP
cord-253024-b393ea2u	267	54	,	,	,
cord-253024-b393ea2u	267	55	LA1	LA1	NNP
cord-253024-b393ea2u	267	56	2	2	CD
cord-253024-b393ea2u	267	57	)	)	-RRB-
cord-253024-b393ea2u	267	58	.	.	.
cord-253024-b393ea2u	268	1	Recombination	recombination	NN
cord-253024-b393ea2u	268	2	rates	rate	NNS
cord-253024-b393ea2u	268	3	were	be	VBD
cord-253024-b393ea2u	268	4	calculated	calculate	VBN
cord-253024-b393ea2u	268	5	through	through	IN
cord-253024-b393ea2u	268	6	the	the	DT
cord-253024-b393ea2u	268	7	S	S	NNP
cord-253024-b393ea2u	268	8	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	268	9	gene	gene	NN
cord-253024-b393ea2u	268	10	,	,	,
cord-253024-b393ea2u	268	11	assuming	assume	VBG
cord-253024-b393ea2u	268	12	the	the	DT
cord-253024-b393ea2u	268	13	group	group	NN
cord-253024-b393ea2u	268	14	F	F	NNP
cord-253024-b393ea2u	268	15	RNA+	RNA+	NNP
cord-253024-b393ea2u	268	16	mutants	mutant	NNS
cord-253024-b393ea2u	268	17	LA7	LA7	NNP
cord-253024-b393ea2u	268	18	and	and	CC
cord-253024-b393ea2u	268	19	NC1	NC1	NNP
cord-253024-b393ea2u	269	1	4	4	LS
cord-253024-b393ea2u	269	2	/	/	SYM
cord-253024-b393ea2u	269	3	NC16	NC16	NNP
cord-253024-b393ea2u	269	4	map	map	VB
cord-253024-b393ea2u	269	5	roughly	roughly	RB
cord-253024-b393ea2u	269	6	4.0	4.0	CD
cord-253024-b393ea2u	269	7	kb	kb	NNS
cord-253024-b393ea2u	269	8	apart	apart	RB
cord-253024-b393ea2u	269	9	.	.	.
cord-253024-b393ea2u	270	1	ase	ase	NNP
cord-253024-b393ea2u	270	2	genes	gene	NNS
cord-253024-b393ea2u	270	3	of	of	IN
cord-253024-b393ea2u	270	4	MHV	MHV	NNP
cord-253024-b393ea2u	270	5	.	.	.
cord-253024-b393ea2u	271	1	Assuming	assume	VBG
cord-253024-b393ea2u	271	2	that	that	IN
cord-253024-b393ea2u	271	3	the	the	DT
cord-253024-b393ea2u	271	4	recombination	recombination	NN
cord-253024-b393ea2u	271	5	frequency	frequency	NN
cord-253024-b393ea2u	271	6	was	be	VBD
cord-253024-b393ea2u	271	7	equivalent	equivalent	JJ
cord-253024-b393ea2u	271	8	throughout	throughout	IN
cord-253024-b393ea2u	271	9	the	the	DT
cord-253024-b393ea2u	271	10	entire	entire	JJ
cord-253024-b393ea2u	271	11	genome	genome	NN
cord-253024-b393ea2u	271	12	,	,	,
cord-253024-b393ea2u	271	13	these	these	DT
cord-253024-b393ea2u	271	14	data	datum	NNS
cord-253024-b393ea2u	271	15	suggested	suggest	VBD
cord-253024-b393ea2u	271	16	that	that	IN
cord-253024-b393ea2u	271	17	the	the	DT
cord-253024-b393ea2u	271	18	recombination	recombination	NN
cord-253024-b393ea2u	271	19	frequency	frequency	NN
cord-253024-b393ea2u	271	20	approached	approach	VBD
cord-253024-b393ea2u	271	21	25	25	CD
cord-253024-b393ea2u	271	22	%	%	NN
cord-253024-b393ea2u	271	23	or	or	CC
cord-253024-b393ea2u	271	24	more	more	JJR
cord-253024-b393ea2u	271	25	.	.	.
cord-253024-b393ea2u	272	1	In	in	IN
cord-253024-b393ea2u	272	2	this	this	DT
cord-253024-b393ea2u	272	3	study	study	NN
cord-253024-b393ea2u	272	4	,	,	,
cord-253024-b393ea2u	272	5	we	-PRON-	PRP
cord-253024-b393ea2u	272	6	have	have	VBP
cord-253024-b393ea2u	272	7	demonstrated	demonstrate	VBN
cord-253024-b393ea2u	272	8	that	that	IN
cord-253024-b393ea2u	272	9	the	the	DT
cord-253024-b393ea2u	272	10	ts	ts	NNP
cord-253024-b393ea2u	272	11	mutants	mutant	NNS
cord-253024-b393ea2u	272	12	from	from	IN
cord-253024-b393ea2u	272	13	a	a	DT
cord-253024-b393ea2u	272	14	RNA+	RNA+	NNP
cord-253024-b393ea2u	272	15	complementation	complementation	NN
cord-253024-b393ea2u	272	16	group	group	NN
cord-253024-b393ea2u	272	17	of	of	IN
cord-253024-b393ea2u	272	18	MHV	MHV	NNP
cord-253024-b393ea2u	272	19	-	-	HYPH
cord-253024-b393ea2u	272	20	A59	A59	NNP
cord-253024-b393ea2u	272	21	can	can	MD
cord-253024-b393ea2u	272	22	also	also	RB
cord-253024-b393ea2u	272	23	be	be	VB
cord-253024-b393ea2u	272	24	arranged	arrange	VBN
cord-253024-b393ea2u	272	25	into	into	IN
cord-253024-b393ea2u	272	26	an	an	DT
cord-253024-b393ea2u	272	27	additive	additive	NN
cord-253024-b393ea2u	272	28	,	,	,
cord-253024-b393ea2u	272	29	linear	linear	NNP
cord-253024-b393ea2u	272	30	,	,	,
cord-253024-b393ea2u	272	31	genetic	genetic	JJ
cord-253024-b393ea2u	272	32	map	map	NN
cord-253024-b393ea2u	272	33	.	.	.
cord-253024-b393ea2u	273	1	Several	several	JJ
cord-253024-b393ea2u	273	2	lines	line	NNS
cord-253024-b393ea2u	273	3	of	of	IN
cord-253024-b393ea2u	273	4	evidence	evidence	NN
cord-253024-b393ea2u	273	5	strongly	strongly	RB
cord-253024-b393ea2u	273	6	suggest	suggest	VBP
cord-253024-b393ea2u	273	7	that	that	IN
cord-253024-b393ea2u	273	8	the	the	DT
cord-253024-b393ea2u	273	9	group	group	NN
cord-253024-b393ea2u	273	10	F	F	NNP
cord-253024-b393ea2u	273	11	RNA+	RNA+	NNP
cord-253024-b393ea2u	273	12	mutants	mutant	NNS
cord-253024-b393ea2u	273	13	map	map	VBP
cord-253024-b393ea2u	273	14	in	in	IN
cord-253024-b393ea2u	273	15	the	the	DT
cord-253024-b393ea2u	273	16	S	S	NNP
cord-253024-b393ea2u	273	17	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	273	18	gene	gene	NN
cord-253024-b393ea2u	273	19	of	of	IN
cord-253024-b393ea2u	273	20	MHV	MHV	NNP
cord-253024-b393ea2u	273	21	-	-	HYPH
cord-253024-b393ea2u	273	22	A59	A59	NNP
cord-253024-b393ea2u	273	23	.	.	.
cord-253024-b393ea2u	274	1	First	first	RB
cord-253024-b393ea2u	274	2	,	,	,
cord-253024-b393ea2u	274	3	the	the	DT
cord-253024-b393ea2u	274	4	group	group	NN
cord-253024-b393ea2u	274	5	F	F	NNP
cord-253024-b393ea2u	274	6	mutants	mutant	NNS
cord-253024-b393ea2u	274	7	do	do	VBP
cord-253024-b393ea2u	274	8	not	not	RB
cord-253024-b393ea2u	274	9	produce	produce	VB
cord-253024-b393ea2u	274	10	syncythium	syncythium	NN
cord-253024-b393ea2u	274	11	when	when	WRB
cord-253024-b393ea2u	274	12	maintained	maintain	VBN
cord-253024-b393ea2u	274	13	at	at	IN
cord-253024-b393ea2u	274	14	the	the	DT
cord-253024-b393ea2u	274	15	restrictive	restrictive	JJ
cord-253024-b393ea2u	274	16	temperature	temperature	NN
cord-253024-b393ea2u	274	17	.	.	.
cord-253024-b393ea2u	275	1	Induction	induction	NN
cord-253024-b393ea2u	275	2	of	of	IN
cord-253024-b393ea2u	275	3	cell	cell	NN
cord-253024-b393ea2u	275	4	fusion	fusion	NN
cord-253024-b393ea2u	275	5	has	have	VBZ
cord-253024-b393ea2u	275	6	been	be	VBN
cord-253024-b393ea2u	275	7	mapped	map	VBN
cord-253024-b393ea2u	275	8	to	to	IN
cord-253024-b393ea2u	275	9	either	either	CC
cord-253024-b393ea2u	275	10	the	the	DT
cord-253024-b393ea2u	275	11	S2	S2	NNP
cord-253024-b393ea2u	275	12	domain	domain	NN
cord-253024-b393ea2u	275	13	of	of	IN
cord-253024-b393ea2u	275	14	the	the	DT
cord-253024-b393ea2u	275	15	bovine	bovine	JJ
cord-253024-b393ea2u	275	16	coronavirus	coronavirus	NN
cord-253024-b393ea2u	275	17	S	s	NN
cord-253024-b393ea2u	275	18	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	275	19	gene	gene	NN
cord-253024-b393ea2u	275	20	(	(	-LRB-
cord-253024-b393ea2u	275	21	Yoo	Yoo	NNP
cord-253024-b393ea2u	275	22	eta/.	eta/.	NNP
cord-253024-b393ea2u	275	23	,	,	,
cord-253024-b393ea2u	275	24	199	199	CD
cord-253024-b393ea2u	275	25	1	1	CD
cord-253024-b393ea2u	275	26	)	)	-RRB-
cord-253024-b393ea2u	275	27	or	or	CC
cord-253024-b393ea2u	275	28	the	the	DT
cord-253024-b393ea2u	275	29	MHV	MHV	NNP
cord-253024-b393ea2u	275	30	Sl	Sl	NNP
cord-253024-b393ea2u	275	31	and	and	CC
cord-253024-b393ea2u	275	32	S2	S2	NNP
cord-253024-b393ea2u	275	33	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	275	34	domains	domain	NNS
cord-253024-b393ea2u	275	35	(	(	-LRB-
cord-253024-b393ea2u	275	36	Gallagher	Gallagher	NNP
cord-253024-b393ea2u	275	37	et	et	NNP
cord-253024-b393ea2u	275	38	al	al	NNP
cord-253024-b393ea2u	275	39	.	.	NNP
cord-253024-b393ea2u	275	40	,	,	,
cord-253024-b393ea2u	275	41	199	199	CD
cord-253024-b393ea2u	275	42	1	1	CD
cord-253024-b393ea2u	275	43	;	;	:
cord-253024-b393ea2u	275	44	Routledge	Routledge	NNP
cord-253024-b393ea2u	275	45	et	et	NNP
cord-253024-b393ea2u	275	46	al	al	NNP
cord-253024-b393ea2u	275	47	.	.	.
cord-253024-b393ea2u	276	1	,	,	,
cord-253024-b393ea2u	276	2	1991	1991	CD
cord-253024-b393ea2u	276	3	;	;	:
cord-253024-b393ea2u	276	4	Weismiller	Weismiller	NNP
cord-253024-b393ea2u	276	5	eta/.	eta/.	CD
cord-253024-b393ea2u	276	6	,	,	,
cord-253024-b393ea2u	276	7	1990	1990	CD
cord-253024-b393ea2u	276	8	)	)	-RRB-
cord-253024-b393ea2u	276	9	.	.	.
cord-253024-b393ea2u	277	1	Second	second	RB
cord-253024-b393ea2u	277	2	,	,	,
cord-253024-b393ea2u	277	3	Tl	Tl	NNP
cord-253024-b393ea2u	277	4	fingerprint	fingerprint	NN
cord-253024-b393ea2u	277	5	analysis	analysis	NN
cord-253024-b393ea2u	277	6	of	of	IN
cord-253024-b393ea2u	277	7	RNA	RNA	NNP
cord-253024-b393ea2u	277	8	recombinant	recombinant	JJ
cord-253024-b393ea2u	277	9	viruses	virus	NNS
cord-253024-b393ea2u	277	10	derived	derive	VBN
cord-253024-b393ea2u	277	11	from	from	IN
cord-253024-b393ea2u	277	12	crosses	crosse	NNS
cord-253024-b393ea2u	277	13	between	between	IN
cord-253024-b393ea2u	277	14	ts	ts	NNP
cord-253024-b393ea2u	277	15	LA7	LA7	NNP
cord-253024-b393ea2u	277	16	or	or	CC
cord-253024-b393ea2u	277	17	LA1	LA1	NNP
cord-253024-b393ea2u	277	18	2	2	CD
cord-253024-b393ea2u	277	19	and	and	CC
cord-253024-b393ea2u	277	20	MHV	MHV	NNP
cord-253024-b393ea2u	277	21	-	-	HYPH
cord-253024-b393ea2u	277	22	JHM	JHM	NNP
cord-253024-b393ea2u	277	23	demonstrated	demonstrate	VBD
cord-253024-b393ea2u	277	24	that	that	IN
cord-253024-b393ea2u	277	25	the	the	DT
cord-253024-b393ea2u	277	26	MHV	MHV	NNP
cord-253024-b393ea2u	277	27	-	-	HYPH
cord-253024-b393ea2u	277	28	A59	A59	NNP
cord-253024-b393ea2u	277	29	Sl	sl	NN
cord-253024-b393ea2u	277	30	domain	domain	NN
cord-253024-b393ea2u	277	31	was	be	VBD
cord-253024-b393ea2u	277	32	always	always	RB
cord-253024-b393ea2u	277	33	replaced	replace	VBN
cord-253024-b393ea2u	277	34	by	by	IN
cord-253024-b393ea2u	277	35	heterologous	heterologous	JJ
cord-253024-b393ea2u	277	36	MHV	MHV	NNP
cord-253024-b393ea2u	277	37	-	-	HYPH
cord-253024-b393ea2u	277	38	JHM	JHM	NNP
cord-253024-b393ea2u	277	39	sequences	sequence	NNS
cord-253024-b393ea2u	277	40	.	.	.
cord-253024-b393ea2u	278	1	Since	since	IN
cord-253024-b393ea2u	278	2	no	no	DT
cord-253024-b393ea2u	278	3	other	other	JJ
cord-253024-b393ea2u	278	4	region	region	NN
cord-253024-b393ea2u	278	5	in	in	IN
cord-253024-b393ea2u	278	6	the	the	DT
cord-253024-b393ea2u	278	7	MHV	MHV	NNP
cord-253024-b393ea2u	278	8	-	-	HYPH
cord-253024-b393ea2u	278	9	A59	A59	NNP
cord-253024-b393ea2u	278	10	genome	genome	NN
cord-253024-b393ea2u	278	11	was	be	VBD
cord-253024-b393ea2u	278	12	uniformly	uniformly	RB
cord-253024-b393ea2u	278	13	replaced	replace	VBN
cord-253024-b393ea2u	278	14	in	in	IN
cord-253024-b393ea2u	278	15	recombinant	recombinant	JJ
cord-253024-b393ea2u	278	16	viruses	virus	NNS
cord-253024-b393ea2u	278	17	,	,	,
cord-253024-b393ea2u	278	18	these	these	DT
cord-253024-b393ea2u	278	19	data	datum	NNS
cord-253024-b393ea2u	278	20	strongly	strongly	RB
cord-253024-b393ea2u	278	21	suggested	suggest	VBD
cord-253024-b393ea2u	278	22	that	that	IN
cord-253024-b393ea2u	278	23	the	the	DT
cord-253024-b393ea2u	278	24	mutations	mutation	NNS
cord-253024-b393ea2u	278	25	in	in	IN
cord-253024-b393ea2u	278	26	these	these	DT
cord-253024-b393ea2u	278	27	ts	t	NNS
cord-253024-b393ea2u	278	28	viruses	virus	NNS
cord-253024-b393ea2u	278	29	reside	reside	VBP
cord-253024-b393ea2u	278	30	within	within	IN
cord-253024-b393ea2u	278	31	the	the	DT
cord-253024-b393ea2u	278	32	Sl	sl	NN
cord-253024-b393ea2u	278	33	coding	coding	NN
cord-253024-b393ea2u	278	34	sequences	sequence	NNS
cord-253024-b393ea2u	278	35	(	(	-LRB-
cord-253024-b393ea2u	279	1	Keck	Keck	NNP
cord-253024-b393ea2u	279	2	et	et	NNP
cord-253024-b393ea2u	279	3	al	al	NNP
cord-253024-b393ea2u	279	4	.	.	.
cord-253024-b393ea2u	280	1	,	,	,
cord-253024-b393ea2u	280	2	1987	1987	CD
cord-253024-b393ea2u	280	3	(	(	-LRB-
cord-253024-b393ea2u	280	4	Keck	Keck	NNP
cord-253024-b393ea2u	280	5	et	et	NNP
cord-253024-b393ea2u	280	6	al	al	NNP
cord-253024-b393ea2u	280	7	.	.	.
cord-253024-b393ea2u	281	1	,	,	,
cord-253024-b393ea2u	281	2	,	,	,
cord-253024-b393ea2u	281	3	1988a	1988a	CD
cord-253024-b393ea2u	281	4	Makino	Makino	NNP
cord-253024-b393ea2u	281	5	et	et	NNP
cord-253024-b393ea2u	281	6	al	al	NNP
cord-253024-b393ea2u	281	7	.	.	NNP
cord-253024-b393ea2u	281	8	,	,	,
cord-253024-b393ea2u	282	1	1986b	1986b	CD
cord-253024-b393ea2u	282	2	Makino	Makino	NNP
cord-253024-b393ea2u	282	3	et	et	NNP
cord-253024-b393ea2u	282	4	al	al	NNP
cord-253024-b393ea2u	282	5	.	.	NNP
cord-253024-b393ea2u	282	6	,	,	,
cord-253024-b393ea2u	282	7	,	,	,
cord-253024-b393ea2u	282	8	1987	1987	CD
cord-253024-b393ea2u	282	9	.	.	.
cord-253024-b393ea2u	283	1	Finally	finally	RB
cord-253024-b393ea2u	283	2	,	,	,
cord-253024-b393ea2u	283	3	sequence	sequence	NN
cord-253024-b393ea2u	283	4	analysis	analysis	NN
cord-253024-b393ea2u	283	5	of	of	IN
cord-253024-b393ea2u	283	6	RNA	RNA	NNP
cord-253024-b393ea2u	283	7	recombinant	recombinant	JJ
cord-253024-b393ea2u	283	8	viruses	virus	NNS
cord-253024-b393ea2u	283	9	derived	derive	VBN
cord-253024-b393ea2u	283	10	from	from	IN
cord-253024-b393ea2u	283	11	these	these	DT
cord-253024-b393ea2u	283	12	mutants	mutant	NNS
cord-253024-b393ea2u	283	13	place	place	VBP
cord-253024-b393ea2u	283	14	the	the	DT
cord-253024-b393ea2u	283	15	ts	ts	NNP
cord-253024-b393ea2u	283	16	allele	allele	NN
cord-253024-b393ea2u	283	17	in	in	IN
cord-253024-b393ea2u	283	18	LA7	LA7	NNP
cord-253024-b393ea2u	283	19	within	within	IN
cord-253024-b393ea2u	283	20	the	the	DT
cord-253024-b393ea2u	283	21	5	5	CD
cord-253024-b393ea2u	283	22	'	'	''
cord-253024-b393ea2u	283	23	most	most	JJS
cord-253024-b393ea2u	283	24	1.1	1.1	CD
cord-253024-b393ea2u	283	25	kb	kb	NNS
cord-253024-b393ea2u	283	26	of	of	IN
cord-253024-b393ea2u	283	27	the	the	DT
cord-253024-b393ea2u	283	28	Sl	sl	NN
cord-253024-b393ea2u	283	29	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	283	30	gene	gene	NN
cord-253024-b393ea2u	283	31	(	(	-LRB-
cord-253024-b393ea2u	283	32	Banner	Banner	NNP
cord-253024-b393ea2u	283	33	et	et	NNP
cord-253024-b393ea2u	283	34	a/.	a/.	NNP
cord-253024-b393ea2u	283	35	,	,	,
cord-253024-b393ea2u	283	36	1990	1990	CD
cord-253024-b393ea2u	283	37	)	)	-RRB-
cord-253024-b393ea2u	283	38	.	.	.
cord-253024-b393ea2u	284	1	While	while	IN
cord-253024-b393ea2u	284	2	these	these	DT
cord-253024-b393ea2u	284	3	data	datum	NNS
cord-253024-b393ea2u	284	4	do	do	VBP
cord-253024-b393ea2u	284	5	not	not	RB
cord-253024-b393ea2u	284	6	definitively	definitively	RB
cord-253024-b393ea2u	284	7	prove	prove	VB
cord-253024-b393ea2u	284	8	that	that	IN
cord-253024-b393ea2u	284	9	the	the	DT
cord-253024-b393ea2u	284	10	Group	Group	NNP
cord-253024-b393ea2u	284	11	F	F	NNP
cord-253024-b393ea2u	284	12	mutants	mutant	NNS
cord-253024-b393ea2u	284	13	map	map	VBP
cord-253024-b393ea2u	284	14	in	in	IN
cord-253024-b393ea2u	284	15	the	the	DT
cord-253024-b393ea2u	284	16	S	S	NNP
cord-253024-b393ea2u	284	17	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	284	18	gene	gene	NN
cord-253024-b393ea2u	284	19	sequence	sequence	NN
cord-253024-b393ea2u	284	20	,	,	,
cord-253024-b393ea2u	284	21	these	these	DT
cord-253024-b393ea2u	284	22	data	datum	NNS
cord-253024-b393ea2u	284	23	have	have	VBP
cord-253024-b393ea2u	284	24	strongly	strongly	RB
cord-253024-b393ea2u	284	25	supported	support	VBN
cord-253024-b393ea2u	284	26	the	the	DT
cord-253024-b393ea2u	284	27	localization	localization	NN
cord-253024-b393ea2u	284	28	of	of	IN
cord-253024-b393ea2u	284	29	these	these	DT
cord-253024-b393ea2u	284	30	mutations	mutation	NNS
cord-253024-b393ea2u	284	31	within	within	IN
cord-253024-b393ea2u	284	32	this	this	DT
cord-253024-b393ea2u	284	33	coding	code	VBG
cord-253024-b393ea2u	284	34	region	region	NN
cord-253024-b393ea2u	284	35	.	.	.
cord-253024-b393ea2u	285	1	On	on	IN
cord-253024-b393ea2u	285	2	the	the	DT
cord-253024-b393ea2u	285	3	basis	basis	NN
cord-253024-b393ea2u	285	4	of	of	IN
cord-253024-b393ea2u	285	5	recombination	recombination	NN
cord-253024-b393ea2u	285	6	mapping	mapping	NN
cord-253024-b393ea2u	285	7	data	datum	NNS
cord-253024-b393ea2u	285	8	,	,	,
cord-253024-b393ea2u	285	9	the	the	DT
cord-253024-b393ea2u	285	10	group	group	NN
cord-253024-b393ea2u	285	11	F	F	NNP
cord-253024-b393ea2u	285	12	RNA+	RNA+	NNP
cord-253024-b393ea2u	285	13	mutants	mutant	NNS
cord-253024-b393ea2u	285	14	appear	appear	VBP
cord-253024-b393ea2u	285	15	to	to	TO
cord-253024-b393ea2u	285	16	map	map	VB
cord-253024-b393ea2u	285	17	into	into	IN
cord-253024-b393ea2u	285	18	two	two	CD
cord-253024-b393ea2u	285	19	discrete	discrete	JJ
cord-253024-b393ea2u	285	20	domains	domain	NNS
cord-253024-b393ea2u	285	21	in	in	IN
cord-253024-b393ea2u	285	22	the	the	DT
cord-253024-b393ea2u	285	23	S	S	NNP
cord-253024-b393ea2u	285	24	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	285	25	gene	gene	NN
cord-253024-b393ea2u	285	26	sequence	sequence	NN
cord-253024-b393ea2u	285	27	(	(	-LRB-
cord-253024-b393ea2u	285	28	Fig	Fig	NNP
cord-253024-b393ea2u	285	29	.	.	.
cord-253024-b393ea2u	285	30	4	4	CD
cord-253024-b393ea2u	285	31	)	)	-RRB-
cord-253024-b393ea2u	285	32	.	.	.
cord-253024-b393ea2u	286	1	Data	datum	NNS
cord-253024-b393ea2u	286	2	from	from	IN
cord-253024-b393ea2u	286	3	our	-PRON-	PRP$
cord-253024-b393ea2u	286	4	laboratory	laboratory	NN
cord-253024-b393ea2u	286	5	and	and	CC
cord-253024-b393ea2u	286	6	others	other	NNS
cord-253024-b393ea2u	286	7	suggest	suggest	VBP
cord-253024-b393ea2u	286	8	that	that	IN
cord-253024-b393ea2u	286	9	the	the	DT
cord-253024-b393ea2u	286	10	Fl	Fl	NNP
cord-253024-b393ea2u	286	11	mutants	mutant	NNS
cord-253024-b393ea2u	286	12	probably	probably	RB
cord-253024-b393ea2u	286	13	map	map	VBP
cord-253024-b393ea2u	286	14	within	within	IN
cord-253024-b393ea2u	286	15	the	the	DT
cord-253024-b393ea2u	286	16	Sl	sl	NN
cord-253024-b393ea2u	286	17	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	286	18	while	while	IN
cord-253024-b393ea2u	286	19	the	the	DT
cord-253024-b393ea2u	286	20	F2	F2	NNP
cord-253024-b393ea2u	286	21	mutants	mutant	NNS
cord-253024-b393ea2u	286	22	map	map	VBP
cord-253024-b393ea2u	286	23	within	within	IN
cord-253024-b393ea2u	286	24	the	the	DT
cord-253024-b393ea2u	286	25	52	52	CD
cord-253024-b393ea2u	286	26	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	286	27	sequences	sequence	NNS
cord-253024-b393ea2u	287	1	(	(	-LRB-
cord-253024-b393ea2u	287	2	Banner	Banner	NNP
cord-253024-b393ea2u	287	3	et	et	NNP
cord-253024-b393ea2u	287	4	a/.	a/.	NNP
cord-253024-b393ea2u	287	5	,	,	,
cord-253024-b393ea2u	287	6	1990	1990	CD
cord-253024-b393ea2u	287	7	;	;	:
cord-253024-b393ea2u	287	8	Keck	Keck	NNP
cord-253024-b393ea2u	287	9	et	et	NNP
cord-253024-b393ea2u	287	10	al	al	NNP
cord-253024-b393ea2u	287	11	.	.	.
cord-253024-b393ea2u	288	1	,	,	,
cord-253024-b393ea2u	288	2	1987	1987	CD
cord-253024-b393ea2u	289	1	Keck	Keck	NNP
cord-253024-b393ea2u	289	2	et	et	NNP
cord-253024-b393ea2u	289	3	al	al	NNP
cord-253024-b393ea2u	289	4	.	.	.
cord-253024-b393ea2u	290	1	,	,	,
cord-253024-b393ea2u	290	2	,	,	,
cord-253024-b393ea2u	290	3	1988a	1988a	CD
cord-253024-b393ea2u	290	4	Makino	Makino	NNP
cord-253024-b393ea2u	290	5	et	et	NNP
cord-253024-b393ea2u	290	6	al	al	NNP
cord-253024-b393ea2u	290	7	,	,	,
cord-253024-b393ea2u	290	8	,	,	,
cord-253024-b393ea2u	290	9	1987	1987	CD
cord-253024-b393ea2u	290	10	Makino	Makino	NNP
cord-253024-b393ea2u	290	11	et	et	NNP
cord-253024-b393ea2u	290	12	al	al	NNP
cord-253024-b393ea2u	290	13	,	,	,
cord-253024-b393ea2u	290	14	,	,	,
cord-253024-b393ea2u	290	15	,	,	,
cord-253024-b393ea2u	290	16	1988	1988	CD
cord-253024-b393ea2u	290	17	.	.	.
cord-253024-b393ea2u	291	1	Sequence	sequence	NN
cord-253024-b393ea2u	291	2	analysis	analysis	NN
cord-253024-b393ea2u	291	3	of	of	IN
cord-253024-b393ea2u	291	4	the	the	DT
cord-253024-b393ea2u	291	5	group	group	NN
cord-253024-b393ea2u	291	6	F	F	NNP
cord-253024-b393ea2u	291	7	RNA+	RNA+	NNP
cord-253024-b393ea2u	291	8	mutants	mutant	NNS
cord-253024-b393ea2u	291	9	and	and	CC
cord-253024-b393ea2u	291	10	revertant	revertant	NN
cord-253024-b393ea2u	291	11	viruses	virus	NNS
cord-253024-b393ea2u	291	12	will	will	MD
cord-253024-b393ea2u	291	13	be	be	VB
cord-253024-b393ea2u	291	14	required	require	VBN
cord-253024-b393ea2u	291	15	to	to	TO
cord-253024-b393ea2u	291	16	definitively	definitively	RB
cord-253024-b393ea2u	291	17	map	map	VB
cord-253024-b393ea2u	291	18	the	the	DT
cord-253024-b393ea2u	291	19	location	location	NN
cord-253024-b393ea2u	291	20	of	of	IN
cord-253024-b393ea2u	291	21	these	these	DT
cord-253024-b393ea2u	291	22	alleles	allele	NNS
cord-253024-b393ea2u	291	23	.	.	.
cord-253024-b393ea2u	292	1	However	however	RB
cord-253024-b393ea2u	292	2	,	,	,
cord-253024-b393ea2u	292	3	since	since	IN
cord-253024-b393ea2u	292	4	mutants	mutant	NNS
cord-253024-b393ea2u	292	5	from	from	IN
cord-253024-b393ea2u	292	6	both	both	DT
cord-253024-b393ea2u	292	7	subgroups	subgroup	NNS
cord-253024-b393ea2u	292	8	are	be	VBP
cord-253024-b393ea2u	292	9	incapable	incapable	JJ
cord-253024-b393ea2u	292	10	of	of	IN
cord-253024-b393ea2u	292	11	producing	produce	VBG
cord-253024-b393ea2u	292	12	syncytium	syncytium	NN
cord-253024-b393ea2u	292	13	at	at	IN
cord-253024-b393ea2u	292	14	the	the	DT
cord-253024-b393ea2u	292	15	restrictive	restrictive	JJ
cord-253024-b393ea2u	292	16	temperature	temperature	NN
cord-253024-b393ea2u	292	17	,	,	,
cord-253024-b393ea2u	292	18	these	these	DT
cord-253024-b393ea2u	292	19	data	datum	NNS
cord-253024-b393ea2u	292	20	suggest	suggest	VBP
cord-253024-b393ea2u	292	21	that	that	IN
cord-253024-b393ea2u	292	22	both	both	DT
cord-253024-b393ea2u	292	23	domains	domain	NNS
cord-253024-b393ea2u	292	24	are	be	VBP
cord-253024-b393ea2u	292	25	important	important	JJ
cord-253024-b393ea2u	292	26	in	in	IN
cord-253024-b393ea2u	292	27	eliciting	elicit	VBG
cord-253024-b393ea2u	292	28	cell	cell	NN
cord-253024-b393ea2u	292	29	fusion	fusion	NN
cord-253024-b393ea2u	292	30	.	.	.
cord-253024-b393ea2u	293	1	Currently	currently	RB
cord-253024-b393ea2u	293	2	,	,	,
cord-253024-b393ea2u	293	3	it	-PRON-	PRP
cord-253024-b393ea2u	293	4	is	be	VBZ
cord-253024-b393ea2u	293	5	unclear	unclear	JJ
cord-253024-b393ea2u	293	6	whether	whether	IN
cord-253024-b393ea2u	293	7	the	the	DT
cord-253024-b393ea2u	293	8	alterations	alteration	NNS
cord-253024-b393ea2u	293	9	in	in	IN
cord-253024-b393ea2u	293	10	the	the	DT
cord-253024-b393ea2u	293	11	Fl	Fl	NNP
cord-253024-b393ea2u	293	12	and	and	CC
cord-253024-b393ea2u	293	13	F2	F2	NNP
cord-253024-b393ea2u	293	14	conditional	conditional	JJ
cord-253024-b393ea2u	293	15	lethal	lethal	JJ
cord-253024-b393ea2u	293	16	mutants	mutant	NNS
cord-253024-b393ea2u	293	17	result	result	VBP
cord-253024-b393ea2u	293	18	in	in	IN
cord-253024-b393ea2u	293	19	a	a	DT
cord-253024-b393ea2u	293	20	temperature	temperature	NN
cord-253024-b393ea2u	293	21	-	-	HYPH
cord-253024-b393ea2u	293	22	sensitive	sensitive	JJ
cord-253024-b393ea2u	293	23	fusogenic	fusogenic	JJ
cord-253024-b393ea2u	293	24	domain	domain	NN
cord-253024-b393ea2u	293	25	or	or	CC
cord-253024-b393ea2u	293	26	alter	alter	VB
cord-253024-b393ea2u	293	27	the	the	DT
cord-253024-b393ea2u	293	28	synthesis	synthesis	NN
cord-253024-b393ea2u	293	29	,	,	,
cord-253024-b393ea2u	293	30	transport	transport	NN
cord-253024-b393ea2u	293	31	,	,	,
cord-253024-b393ea2u	293	32	and	and	CC
cord-253024-b393ea2u	293	33	surface	surface	NN
cord-253024-b393ea2u	293	34	expression	expression	NN
cord-253024-b393ea2u	293	35	of	of	IN
cord-253024-b393ea2u	293	36	the	the	DT
cord-253024-b393ea2u	293	37	S	S	NNP
cord-253024-b393ea2u	293	38	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	293	39	gene	gene	NN
cord-253024-b393ea2u	293	40	.	.	.
cord-253024-b393ea2u	294	1	The	the	DT
cord-253024-b393ea2u	294	2	recombination	recombination	NN
cord-253024-b393ea2u	294	3	frequency	frequency	NN
cord-253024-b393ea2u	294	4	for	for	IN
cord-253024-b393ea2u	294	5	the	the	DT
cord-253024-b393ea2u	294	6	entire	entire	JJ
cord-253024-b393ea2u	294	7	MHV	MHV	NNP
cord-253024-b393ea2u	294	8	genome	genome	NN
cord-253024-b393ea2u	294	9	has	have	VBZ
cord-253024-b393ea2u	294	10	been	be	VBN
cord-253024-b393ea2u	294	11	estimated	estimate	VBN
cord-253024-b393ea2u	294	12	to	to	TO
cord-253024-b393ea2u	294	13	approach	approach	VB
cord-253024-b393ea2u	294	14	25	25	CD
cord-253024-b393ea2u	294	15	%	%	NN
cord-253024-b393ea2u	294	16	or	or	CC
cord-253024-b393ea2u	294	17	more	more	JJR
cord-253024-b393ea2u	294	18	.	.	.
cord-253024-b393ea2u	295	1	These	these	DT
cord-253024-b393ea2u	295	2	estimates	estimate	NNS
cord-253024-b393ea2u	295	3	were	be	VBD
cord-253024-b393ea2u	295	4	based	base	VBN
cord-253024-b393ea2u	295	5	on	on	IN
cord-253024-b393ea2u	295	6	the	the	DT
cord-253024-b393ea2u	295	7	assumption	assumption	NN
cord-253024-b393ea2u	295	8	that	that	IN
cord-253024-b393ea2u	295	9	the	the	DT
cord-253024-b393ea2u	295	10	recombination	recombination	NN
cord-253024-b393ea2u	295	11	frequency	frequency	NN
cord-253024-b393ea2u	295	12	was	be	VBD
cord-253024-b393ea2u	295	13	uniform	uniform	JJ
cord-253024-b393ea2u	295	14	and	and	CC
cord-253024-b393ea2u	295	15	approached	approach	VBD
cord-253024-b393ea2u	295	16	10/0	10/0	NNP
cord-253024-b393ea2u	295	17	/	/	SYM
cord-253024-b393ea2u	295	18	l	l	NNP
cord-253024-b393ea2u	295	19	300	300	CD
cord-253024-b393ea2u	295	20	-	-	HYPH
cord-253024-b393ea2u	295	21	1400	1400	CD
cord-253024-b393ea2u	295	22	nucleotides	nucleotide	NNS
cord-253024-b393ea2u	295	23	of	of	IN
cord-253024-b393ea2u	295	24	dsRNA	dsrna	NN
cord-253024-b393ea2u	295	25	throughout	throughout	IN
cord-253024-b393ea2u	295	26	the	the	DT
cord-253024-b393ea2u	295	27	entire	entire	JJ
cord-253024-b393ea2u	295	28	32-kb	32-kb	CD
cord-253024-b393ea2u	295	29	MHV	MHV	NNP
cord-253024-b393ea2u	295	30	genome	genome	NN
cord-253024-b393ea2u	295	31	.	.	.
cord-253024-b393ea2u	296	1	However	however	RB
cord-253024-b393ea2u	296	2	,	,	,
cord-253024-b393ea2u	296	3	recombination	recombination	NN
cord-253024-b393ea2u	296	4	rates	rate	NNS
cord-253024-b393ea2u	296	5	measured	measure	VBN
cord-253024-b393ea2u	296	6	within	within	IN
cord-253024-b393ea2u	296	7	the	the	DT
cord-253024-b393ea2u	296	8	polymerase	polymerase	NN
cord-253024-b393ea2u	296	9	region	region	NN
cord-253024-b393ea2u	296	10	and	and	CC
cord-253024-b393ea2u	296	11	S	S	NNP
cord-253024-b393ea2u	296	12	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	296	13	gene	gene	NN
cord-253024-b393ea2u	296	14	were	be	VBD
cord-253024-b393ea2u	296	15	estimated	estimate	VBN
cord-253024-b393ea2u	296	16	to	to	TO
cord-253024-b393ea2u	296	17	occur	occur	VB
cord-253024-b393ea2u	296	18	at	at	IN
cord-253024-b393ea2u	296	19	frequencies	frequency	NNS
cord-253024-b393ea2u	296	20	of	of	IN
cord-253024-b393ea2u	296	21	about	about	RB
cord-253024-b393ea2u	296	22	1%/l	1%/l	CD
cord-253024-b393ea2u	296	23	800	800	CD
cord-253024-b393ea2u	296	24	-	-	CD
cord-253024-b393ea2u	296	25	2500	2500	CD
cord-253024-b393ea2u	296	26	and	and	CC
cord-253024-b393ea2u	296	27	1%/575	1%/575	CD
cord-253024-b393ea2u	296	28	-	-	SYM
cord-253024-b393ea2u	296	29	667	667	CD
cord-253024-b393ea2u	296	30	bp	bp	NNP
cord-253024-b393ea2u	296	31	of	of	IN
cord-253024-b393ea2u	296	32	dsRNA	dsRNA	NNS
cord-253024-b393ea2u	296	33	,	,	,
cord-253024-b393ea2u	296	34	respectively	respectively	RB
cord-253024-b393ea2u	296	35	(	(	-LRB-
cord-253024-b393ea2u	296	36	Fig	fig	NN
cord-253024-b393ea2u	296	37	.	.	.
cord-253024-b393ea2u	296	38	 	 	_SP
cord-253024-b393ea2u	296	39	5	5	CD
cord-253024-b393ea2u	296	40	)	)	-RRB-
cord-253024-b393ea2u	296	41	.	.	.
cord-253024-b393ea2u	297	1	Recombination	recombination	NN
cord-253024-b393ea2u	297	2	rates	rate	NNS
cord-253024-b393ea2u	297	3	across	across	IN
cord-253024-b393ea2u	297	4	the	the	DT
cord-253024-b393ea2u	297	5	p30/	p30/	XX
cord-253024-b393ea2u	298	1	HE	HE	NNP
cord-253024-b393ea2u	298	2	genes	gene	NNS
cord-253024-b393ea2u	298	3	were	be	VBD
cord-253024-b393ea2u	298	4	estimated	estimate	VBN
cord-253024-b393ea2u	298	5	to	to	TO
cord-253024-b393ea2u	298	6	occur	occur	VB
cord-253024-b393ea2u	298	7	at	at	IN
cord-253024-b393ea2u	298	8	about	about	RB
cord-253024-b393ea2u	298	9	19	19	CD
cord-253024-b393ea2u	298	10	/	/	SYM
cord-253024-b393ea2u	298	11	o	o	NN
cord-253024-b393ea2u	298	12	/	/	SYM
cord-253024-b393ea2u	298	13	l	l	NN
cord-253024-b393ea2u	298	14	100-l	100-l	CD
cord-253024-b393ea2u	298	15	400	400	CD
cord-253024-b393ea2u	298	16	bp	bp	NNP
cord-253024-b393ea2u	298	17	of	of	IN
cord-253024-b393ea2u	298	18	dsRNA	dsrna	NN
cord-253024-b393ea2u	298	19	.	.	.
cord-253024-b393ea2u	299	1	Currently	currently	RB
cord-253024-b393ea2u	299	2	,	,	,
cord-253024-b393ea2u	299	3	our	-PRON-	PRP$
cord-253024-b393ea2u	299	4	best	good	JJS
cord-253024-b393ea2u	299	5	estimate	estimate	NN
cord-253024-b393ea2u	299	6	for	for	IN
cord-253024-b393ea2u	299	7	an	an	DT
cord-253024-b393ea2u	299	8	MHV	MHV	NNP
cord-253024-b393ea2u	299	9	recombination	recombination	NN
cord-253024-b393ea2u	299	10	frequency	frequency	NN
cord-253024-b393ea2u	299	11	was	be	VBD
cord-253024-b393ea2u	299	12	measured	measure	VBN
cord-253024-b393ea2u	299	13	within	within	IN
cord-253024-b393ea2u	299	14	the	the	DT
cord-253024-b393ea2u	299	15	S	S	NNP
cord-253024-b393ea2u	299	16	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	299	17	gene	gene	NN
cord-253024-b393ea2u	299	18	because	because	IN
cord-253024-b393ea2u	299	19	these	these	DT
cord-253024-b393ea2u	299	20	mutations	mutation	NNS
cord-253024-b393ea2u	299	21	must	must	MD
cord-253024-b393ea2u	299	22	have	have	VB
cord-253024-b393ea2u	299	23	resided	reside	VBN
cord-253024-b393ea2u	299	24	within	within	IN
cord-253024-b393ea2u	299	25	a	a	DT
cord-253024-b393ea2u	299	26	4.0-kb	4.0-kb	CD
cord-253024-b393ea2u	299	27	stretch	stretch	NN
cord-253024-b393ea2u	299	28	of	of	IN
cord-253024-b393ea2u	299	29	RNA	RNA	NNP
cord-253024-b393ea2u	299	30	.	.	.
cord-253024-b393ea2u	300	1	Moreover	moreover	RB
cord-253024-b393ea2u	300	2	,	,	,
cord-253024-b393ea2u	300	3	the	the	DT
cord-253024-b393ea2u	300	4	frequency	frequency	NN
cord-253024-b393ea2u	300	5	of	of	IN
cord-253024-b393ea2u	300	6	recombination	recombination	NN
cord-253024-b393ea2u	300	7	was	be	VBD
cord-253024-b393ea2u	300	8	internally	internally	RB
cord-253024-b393ea2u	300	9	consistent	consistent	JJ
cord-253024-b393ea2u	300	10	between	between	IN
cord-253024-b393ea2u	300	11	several	several	JJ
cord-253024-b393ea2u	300	12	independent	independent	JJ
cord-253024-b393ea2u	300	13	crosses	crosse	NNS
cord-253024-b393ea2u	300	14	within	within	IN
cord-253024-b393ea2u	300	15	a	a	DT
cord-253024-b393ea2u	300	16	single	single	JJ
cord-253024-b393ea2u	300	17	complementation	complementation	NN
cord-253024-b393ea2u	300	18	group	group	NN
cord-253024-b393ea2u	300	19	(	(	-LRB-
cord-253024-b393ea2u	300	20	Table	Table	NNP
cord-253024-b393ea2u	300	21	3	3	CD
cord-253024-b393ea2u	300	22	)	)	-RRB-
cord-253024-b393ea2u	300	23	.	.	.
cord-253024-b393ea2u	301	1	While	while	IN
cord-253024-b393ea2u	301	2	definitive	definitive	JJ
cord-253024-b393ea2u	301	3	proof	proof	NN
cord-253024-b393ea2u	301	4	will	will	MD
cord-253024-b393ea2u	301	5	require	require	VB
cord-253024-b393ea2u	301	6	the	the	DT
cord-253024-b393ea2u	301	7	identification	identification	NN
cord-253024-b393ea2u	301	8	of	of	IN
cord-253024-b393ea2u	301	9	the	the	DT
cord-253024-b393ea2u	301	10	exact	exact	JJ
cord-253024-b393ea2u	301	11	location	location	NN
cord-253024-b393ea2u	301	12	of	of	IN
cord-253024-b393ea2u	301	13	the	the	DT
cord-253024-b393ea2u	301	14	ts	ts	NNP
cord-253024-b393ea2u	301	15	alleles	allele	NNS
cord-253024-b393ea2u	301	16	used	use	VBN
cord-253024-b393ea2u	301	17	in	in	IN
cord-253024-b393ea2u	301	18	these	these	DT
cord-253024-b393ea2u	301	19	studies	study	NNS
cord-253024-b393ea2u	301	20	,	,	,
cord-253024-b393ea2u	301	21	these	these	DT
cord-253024-b393ea2u	301	22	data	datum	NNS
cord-253024-b393ea2u	301	23	suggest	suggest	VBP
cord-253024-b393ea2u	301	24	that	that	IN
cord-253024-b393ea2u	301	25	the	the	DT
cord-253024-b393ea2u	301	26	recombination	recombination	NN
cord-253024-b393ea2u	301	27	frequency	frequency	NN
cord-253024-b393ea2u	301	28	varies	vary	VBZ
cord-253024-b393ea2u	301	29	in	in	IN
cord-253024-b393ea2u	301	30	different	different	JJ
cord-253024-b393ea2u	301	31	portions	portion	NNS
cord-253024-b393ea2u	301	32	of	of	IN
cord-253024-b393ea2u	301	33	the	the	DT
cord-253024-b393ea2u	301	34	genome	genome	NN
cord-253024-b393ea2u	301	35	and	and	CC
cord-253024-b393ea2u	301	36	is	be	VBZ
cord-253024-b393ea2u	301	37	roughly	roughly	RB
cord-253024-b393ea2u	301	38	threefold	threefold	RB
cord-253024-b393ea2u	301	39	higher	higher	RBR
cord-253024-b393ea2u	301	40	in	in	IN
cord-253024-b393ea2u	301	41	the	the	DT
cord-253024-b393ea2u	301	42	S	S	NNP
cord-253024-b393ea2u	301	43	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	301	44	gene	gene	NN
cord-253024-b393ea2u	301	45	.	.	.
cord-253024-b393ea2u	302	1	Several	several	JJ
cord-253024-b393ea2u	302	2	explanations	explanation	NNS
cord-253024-b393ea2u	302	3	could	could	MD
cord-253024-b393ea2u	302	4	account	account	VB
cord-253024-b393ea2u	302	5	for	for	IN
cord-253024-b393ea2u	302	6	an	an	DT
cord-253024-b393ea2u	302	7	increase	increase	NN
cord-253024-b393ea2u	302	8	in	in	IN
cord-253024-b393ea2u	302	9	the	the	DT
cord-253024-b393ea2u	302	10	recombination	recombination	NN
cord-253024-b393ea2u	302	11	frequency	frequency	NN
cord-253024-b393ea2u	302	12	within	within	IN
cord-253024-b393ea2u	302	13	the	the	DT
cord-253024-b393ea2u	302	14	S	S	NNP
cord-253024-b393ea2u	302	15	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	302	16	gene	gene	NN
cord-253024-b393ea2u	302	17	.	.	.
cord-253024-b393ea2u	303	1	First	first	RB
cord-253024-b393ea2u	303	2	,	,	,
cord-253024-b393ea2u	303	3	the	the	DT
cord-253024-b393ea2u	303	4	recombination	recombination	NN
cord-253024-b393ea2u	303	5	rate	rate	NN
cord-253024-b393ea2u	303	6	through	through	IN
cord-253024-b393ea2u	303	7	the	the	DT
cord-253024-b393ea2u	303	8	FIG	fig	NN
cord-253024-b393ea2u	303	9	.	.	.
cord-253024-b393ea2u	304	1	6	6	LS
cord-253024-b393ea2u	304	2	.	.	.
cord-253024-b393ea2u	305	1	PCR	PCR	NNP
cord-253024-b393ea2u	305	2	ampliflcatron	ampliflcatron	NNP
cord-253024-b393ea2u	305	3	and	and	CC
cord-253024-b393ea2u	305	4	size	size	NN
cord-253024-b393ea2u	305	5	analysrs	analysr	NNS
cord-253024-b393ea2u	305	6	of	of	IN
cord-253024-b393ea2u	305	7	the	the	DT
cord-253024-b393ea2u	305	8	S	S	NNP
cord-253024-b393ea2u	305	9	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	305	10	gene	gene	NN
cord-253024-b393ea2u	305	11	of	of	IN
cord-253024-b393ea2u	305	12	wildtype	wildtype	NN
cord-253024-b393ea2u	305	13	,	,	,
cord-253024-b393ea2u	305	14	Ts	Ts	NNP
cord-253024-b393ea2u	305	15	,	,	,
cord-253024-b393ea2u	305	16	recombmant	recombmant	JJ
cord-253024-b393ea2u	305	17	,	,	,
cord-253024-b393ea2u	305	18	and	and	CC
cord-253024-b393ea2u	305	19	revertant	revertant	NN
cord-253024-b393ea2u	305	20	viruses	virus	NNS
cord-253024-b393ea2u	305	21	.	.	.
cord-253024-b393ea2u	306	1	Cultures	culture	NNS
cord-253024-b393ea2u	306	2	of	of	IN
cord-253024-b393ea2u	306	3	cells	cell	NNS
cord-253024-b393ea2u	306	4	were	be	VBD
cord-253024-b393ea2u	306	5	infected	infect	VBN
cord-253024-b393ea2u	306	6	with	with	IN
cord-253024-b393ea2u	306	7	different	different	JJ
cord-253024-b393ea2u	306	8	strains	strain	NNS
cord-253024-b393ea2u	306	9	of	of	IN
cord-253024-b393ea2u	306	10	MHV	mhv	NN
cord-253024-b393ea2u	306	11	ts	ts	NN
cord-253024-b393ea2u	306	12	mutants	mutant	NNS
cord-253024-b393ea2u	306	13	,	,	,
cord-253024-b393ea2u	306	14	recombinant	recombinant	JJ
cord-253024-b393ea2u	306	15	viruses	virus	NNS
cord-253024-b393ea2u	306	16	,	,	,
cord-253024-b393ea2u	306	17	or	or	CC
cord-253024-b393ea2u	306	18	revertant	revertant	NN
cord-253024-b393ea2u	306	19	viruses	virus	NNS
cord-253024-b393ea2u	306	20	.	.	.
cord-253024-b393ea2u	307	1	The	the	DT
cord-253024-b393ea2u	307	2	Intercellular	Intercellular	NNP
cord-253024-b393ea2u	307	3	RNA	RNA	NNP
cord-253024-b393ea2u	307	4	was	be	VBD
cord-253024-b393ea2u	307	5	extracted	extract	VBN
cord-253024-b393ea2u	307	6	and	and	CC
cord-253024-b393ea2u	307	7	used	use	VBN
cord-253024-b393ea2u	307	8	as	as	IN
cord-253024-b393ea2u	307	9	template	template	NN
cord-253024-b393ea2u	307	10	for	for	IN
cord-253024-b393ea2u	307	11	reverse	reverse	JJ
cord-253024-b393ea2u	307	12	transcription	transcription	NN
cord-253024-b393ea2u	307	13	and	and	CC
cord-253024-b393ea2u	307	14	30	30	CD
cord-253024-b393ea2u	307	15	rounds	round	NNS
cord-253024-b393ea2u	307	16	of	of	IN
cord-253024-b393ea2u	307	17	PCR	PCR	NNP
cord-253024-b393ea2u	307	18	amplification	amplification	NN
cord-253024-b393ea2u	307	19	usrng	usrng	VBP
cord-253024-b393ea2u	307	20	the	the	DT
cord-253024-b393ea2u	307	21	TAQ	taq	NN
cord-253024-b393ea2u	307	22	polymerase	polymerase	NN
cord-253024-b393ea2u	307	23	and	and	CC
cord-253024-b393ea2u	307	24	different	different	JJ
cord-253024-b393ea2u	307	25	primer	primer	NN
cord-253024-b393ea2u	307	26	parrs	parr	NNS
cord-253024-b393ea2u	307	27	.	.	.
cord-253024-b393ea2u	308	1	(	(	-LRB-
cord-253024-b393ea2u	308	2	A	a	NN
cord-253024-b393ea2u	308	3	)	)	-RRB-
cord-253024-b393ea2u	308	4	PCR	PCR	NNP
cord-253024-b393ea2u	308	5	products	product	NNS
cord-253024-b393ea2u	308	6	spanning	span	VBG
cord-253024-b393ea2u	308	7	the	the	DT
cord-253024-b393ea2u	308	8	hypervariable	hypervariable	JJ
cord-253024-b393ea2u	308	9	region	region	NN
cord-253024-b393ea2u	308	10	of	of	IN
cord-253024-b393ea2u	308	11	MHVA59	MHVA59	NNP
cord-253024-b393ea2u	308	12	,	,	,
cord-253024-b393ea2u	308	13	JHM	JHM	NNP
cord-253024-b393ea2u	308	14	,	,	,
cord-253024-b393ea2u	308	15	and	and	CC
cord-253024-b393ea2u	308	16	MHV-2	mhv-2	NN
cord-253024-b393ea2u	309	1	(	(	-LRB-
cord-253024-b393ea2u	309	2	642	642	CD
cord-253024-b393ea2u	309	3	-	-	HYPH
cord-253024-b393ea2u	309	4	2155	2155	CD
cord-253024-b393ea2u	309	5	)	)	-RRB-
cord-253024-b393ea2u	309	6	(	(	-LRB-
cord-253024-b393ea2u	309	7	lanes	lane	NNS
cord-253024-b393ea2u	309	8	2	2	CD
cord-253024-b393ea2u	309	9	-	-	SYM
cord-253024-b393ea2u	309	10	4	4	CD
cord-253024-b393ea2u	309	11	,	,	,
cord-253024-b393ea2u	309	12	respectively	respectively	RB
cord-253024-b393ea2u	309	13	;	;	:
cord-253024-b393ea2u	309	14	lane	lane	NN
cord-253024-b393ea2u	309	15	5	5	CD
cord-253024-b393ea2u	309	16	,	,	,
cord-253024-b393ea2u	309	17	uninfected	uninfected	JJ
cord-253024-b393ea2u	309	18	control	control	NN
cord-253024-b393ea2u	309	19	)	)	-RRB-
cord-253024-b393ea2u	309	20	;	;	:
cord-253024-b393ea2u	309	21	(	(	-LRB-
cord-253024-b393ea2u	309	22	B	b	NN
cord-253024-b393ea2u	309	23	)	)	-RRB-
cord-253024-b393ea2u	309	24	Southern	southern	JJ
cord-253024-b393ea2u	309	25	blot	blot	NN
cord-253024-b393ea2u	309	26	analysis	analysis	NN
cord-253024-b393ea2u	309	27	of	of	IN
cord-253024-b393ea2u	309	28	the	the	DT
cord-253024-b393ea2u	309	29	PCR	PCR	NNP
cord-253024-b393ea2u	309	30	products	product	NNS
cord-253024-b393ea2u	309	31	shown	show	VBN
cord-253024-b393ea2u	309	32	in	in	IN
cord-253024-b393ea2u	309	33	(	(	-LRB-
cord-253024-b393ea2u	309	34	A	a	NN
cord-253024-b393ea2u	309	35	)	)	-RRB-
cord-253024-b393ea2u	309	36	using	use	VBG
cord-253024-b393ea2u	309	37	an	an	DT
cord-253024-b393ea2u	309	38	Internal	Internal	NNP
cord-253024-b393ea2u	309	39	ollgomer	ollgomer	NN
cord-253024-b393ea2u	309	40	probe	probe	NN
cord-253024-b393ea2u	309	41	;	;	,
cord-253024-b393ea2u	309	42	(	(	-LRB-
cord-253024-b393ea2u	309	43	C	C	NNP
cord-253024-b393ea2u	309	44	)	)	-RRB-
cord-253024-b393ea2u	309	45	PCR	PCR	NNP
cord-253024-b393ea2u	309	46	products	product	NNS
cord-253024-b393ea2u	309	47	spanning	span	VBG
cord-253024-b393ea2u	309	48	the	the	DT
cord-253024-b393ea2u	309	49	hypervariable	hypervariable	JJ
cord-253024-b393ea2u	309	50	region	region	NN
cord-253024-b393ea2u	309	51	In	in	IN
cord-253024-b393ea2u	309	52	the	the	DT
cord-253024-b393ea2u	309	53	S	S	NNP
cord-253024-b393ea2u	309	54	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	309	55	gene	gene	NN
cord-253024-b393ea2u	309	56	of	of	IN
cord-253024-b393ea2u	309	57	MHV	MHV	NNP
cord-253024-b393ea2u	309	58	-	-	HYPH
cord-253024-b393ea2u	309	59	A59	A59	NNP
cord-253024-b393ea2u	309	60	(	(	-LRB-
cord-253024-b393ea2u	309	61	lane	lane	NN
cord-253024-b393ea2u	309	62	2	2	CD
cord-253024-b393ea2u	309	63	)	)	-RRB-
cord-253024-b393ea2u	309	64	and	and	CC
cord-253024-b393ea2u	309	65	RNA	RNA	NNP
cord-253024-b393ea2u	309	66	recombinant	recombinant	JJ
cord-253024-b393ea2u	309	67	viruses	virus	NNS
cord-253024-b393ea2u	309	68	derived	derive	VBN
cord-253024-b393ea2u	309	69	from	from	IN
cord-253024-b393ea2u	309	70	NC1	NC1	NNP
cord-253024-b393ea2u	310	1	7	7	CD
cord-253024-b393ea2u	310	2	x	x	SYM
cord-253024-b393ea2u	310	3	NC1	NC1	NNP
cord-253024-b393ea2u	310	4	6	6	CD
cord-253024-b393ea2u	310	5	[	[	-LRB-
cord-253024-b393ea2u	310	6	lanes	lane	NNS
cord-253024-b393ea2u	310	7	3	3	CD
cord-253024-b393ea2u	310	8	-	-	SYM
cord-253024-b393ea2u	310	9	5	5	CD
cord-253024-b393ea2u	310	10	)	)	-RRB-
cord-253024-b393ea2u	311	1	NC6	NC6	NNP
cord-253024-b393ea2u	311	2	x	x	NNP
cord-253024-b393ea2u	311	3	LA7	LA7	NNP
cord-253024-b393ea2u	311	4	(	(	-LRB-
cord-253024-b393ea2u	311	5	lanes	lane	NNS
cord-253024-b393ea2u	311	6	6	6	CD
cord-253024-b393ea2u	311	7	-	-	SYM
cord-253024-b393ea2u	311	8	8	8	CD
cord-253024-b393ea2u	311	9	)	)	-RRB-
cord-253024-b393ea2u	311	10	and	and	CC
cord-253024-b393ea2u	311	11	NC1	NC1	NNP
cord-253024-b393ea2u	311	12	6	6	CD
cord-253024-b393ea2u	311	13	X	X	NNP
cord-253024-b393ea2u	311	14	LA7	LA7	NNP
cord-253024-b393ea2u	311	15	(	(	-LRB-
cord-253024-b393ea2u	311	16	lanes	lane	NNS
cord-253024-b393ea2u	311	17	1	1	CD
cord-253024-b393ea2u	311	18	O	o	NN
cord-253024-b393ea2u	311	19	-	-	HYPH
cord-253024-b393ea2u	311	20	l	l	NN
cord-253024-b393ea2u	311	21	2	2	CD
cord-253024-b393ea2u	311	22	)	)	-RRB-
cord-253024-b393ea2u	311	23	;	;	:
cord-253024-b393ea2u	311	24	(	(	-LRB-
cord-253024-b393ea2u	311	25	D	D	NNP
cord-253024-b393ea2u	311	26	)	)	-RRB-
cord-253024-b393ea2u	311	27	PCR	PCR	NNP
cord-253024-b393ea2u	311	28	products	product	NNS
cord-253024-b393ea2u	311	29	spanning	span	VBG
cord-253024-b393ea2u	311	30	the	the	DT
cord-253024-b393ea2u	311	31	C	C	NNP
cord-253024-b393ea2u	311	32	(	(	-LRB-
cord-253024-b393ea2u	311	33	lanes	lane	NNS
cord-253024-b393ea2u	311	34	2	2	CD
cord-253024-b393ea2u	311	35	-	-	SYM
cord-253024-b393ea2u	311	36	1	1	CD
cord-253024-b393ea2u	311	37	1	1	CD
cord-253024-b393ea2u	311	38	)	)	-RRB-
cord-253024-b393ea2u	311	39	and	and	CC
cord-253024-b393ea2u	311	40	N	N	NNP
cord-253024-b393ea2u	311	41	(	(	-LRB-
cord-253024-b393ea2u	311	42	lanes	lane	NNS
cord-253024-b393ea2u	311	43	12	12	CD
cord-253024-b393ea2u	311	44	-	-	SYM
cord-253024-b393ea2u	311	45	21	21	CD
cord-253024-b393ea2u	311	46	)	)	-RRB-
cord-253024-b393ea2u	311	47	terminl	terminl	NNS
cord-253024-b393ea2u	311	48	of	of	IN
cord-253024-b393ea2u	311	49	MHV	MHV	NNP
cord-253024-b393ea2u	311	50	-	-	HYPH
cord-253024-b393ea2u	311	51	A59	A59	NNP
cord-253024-b393ea2u	311	52	(	(	-LRB-
cord-253024-b393ea2u	311	53	lanes	lane	NNS
cord-253024-b393ea2u	311	54	2	2	CD
cord-253024-b393ea2u	311	55	,	,	,
cord-253024-b393ea2u	311	56	12	12	CD
cord-253024-b393ea2u	311	57	)	)	-RRB-
cord-253024-b393ea2u	311	58	and	and	CC
cord-253024-b393ea2u	311	59	RNA	RNA	NNP
cord-253024-b393ea2u	311	60	recombinant	recombinant	JJ
cord-253024-b393ea2u	311	61	viruses	virus	NNS
cord-253024-b393ea2u	311	62	derived	derive	VBN
cord-253024-b393ea2u	311	63	from	from	IN
cord-253024-b393ea2u	311	64	NC1	NC1	NNP
cord-253024-b393ea2u	311	65	7	7	NNP
cord-253024-b393ea2u	311	66	X	X	NNP
cord-253024-b393ea2u	311	67	NC1	NC1	NNP
cord-253024-b393ea2u	311	68	6	6	CD
cord-253024-b393ea2u	311	69	(	(	-LRB-
cord-253024-b393ea2u	311	70	lanes	lane	NNS
cord-253024-b393ea2u	311	71	3	3	CD
cord-253024-b393ea2u	311	72	-	-	SYM
cord-253024-b393ea2u	311	73	5	5	CD
cord-253024-b393ea2u	311	74	and	and	CC
cord-253024-b393ea2u	311	75	13-l	13-l	CD
cord-253024-b393ea2u	311	76	5	5	CD
cord-253024-b393ea2u	311	77	)	)	-RRB-
cord-253024-b393ea2u	311	78	.	.	.
cord-253024-b393ea2u	312	1	NC6	NC6	NNP
cord-253024-b393ea2u	312	2	X	X	NNP
cord-253024-b393ea2u	312	3	LA7	LA7	NNP
cord-253024-b393ea2u	312	4	(	(	-LRB-
cord-253024-b393ea2u	312	5	lanes	lane	NNS
cord-253024-b393ea2u	312	6	6	6	CD
cord-253024-b393ea2u	312	7	-	-	SYM
cord-253024-b393ea2u	312	8	8	8	CD
cord-253024-b393ea2u	312	9	and	and	CC
cord-253024-b393ea2u	312	10	16-l	16-l	CD
cord-253024-b393ea2u	312	11	8)	8)	NNP
cord-253024-b393ea2u	312	12	and	and	CC
cord-253024-b393ea2u	312	13	NC1	NC1	NNP
cord-253024-b393ea2u	312	14	6	6	CD
cord-253024-b393ea2u	312	15	X	X	NNP
cord-253024-b393ea2u	312	16	LA7	LA7	NNP
cord-253024-b393ea2u	312	17	(	(	-LRB-
cord-253024-b393ea2u	312	18	lanes	lane	NNS
cord-253024-b393ea2u	312	19	9-l	9-l	CD
cord-253024-b393ea2u	312	20	1	1	CD
cord-253024-b393ea2u	312	21	and	and	CC
cord-253024-b393ea2u	312	22	19921	19921	CD
cord-253024-b393ea2u	312	23	)	)	-RRB-
cord-253024-b393ea2u	312	24	;	;	:
cord-253024-b393ea2u	312	25	(	(	-LRB-
cord-253024-b393ea2u	312	26	E	e	NN
cord-253024-b393ea2u	312	27	)	)	-RRB-
cord-253024-b393ea2u	312	28	PCR	PCR	NNP
cord-253024-b393ea2u	312	29	products	product	NNS
cord-253024-b393ea2u	312	30	spanning	span	VBG
cord-253024-b393ea2u	312	31	the	the	DT
cord-253024-b393ea2u	312	32	hypervariable	hypervariable	JJ
cord-253024-b393ea2u	312	33	region	region	NN
cord-253024-b393ea2u	312	34	of	of	IN
cord-253024-b393ea2u	312	35	NC	NC	NNP
cord-253024-b393ea2u	312	36	17	17	CD
cord-253024-b393ea2u	312	37	(	(	-LRB-
cord-253024-b393ea2u	312	38	lane	lane	NNP
cord-253024-b393ea2u	312	39	2	2	CD
cord-253024-b393ea2u	312	40	)	)	-RRB-
cord-253024-b393ea2u	312	41	and	and	CC
cord-253024-b393ea2u	312	42	four	four	CD
cord-253024-b393ea2u	312	43	different	different	JJ
cord-253024-b393ea2u	312	44	revertant	revertant	NN
cord-253024-b393ea2u	312	45	viruses	virus	NNS
cord-253024-b393ea2u	312	46	(	(	-LRB-
cord-253024-b393ea2u	312	47	lanes	lane	NNS
cord-253024-b393ea2u	312	48	3	3	CD
cord-253024-b393ea2u	312	49	-	-	SYM
cord-253024-b393ea2u	312	50	6	6	CD
cord-253024-b393ea2u	312	51	)	)	-RRB-
cord-253024-b393ea2u	312	52	.	.	.
cord-253024-b393ea2u	313	1	Lane	lane	NN
cord-253024-b393ea2u	313	2	1	1	CD
cord-253024-b393ea2u	313	3	contains	contain	VBZ
cord-253024-b393ea2u	313	4	DNA	dna	NN
cord-253024-b393ea2u	313	5	markers	marker	NNS
cord-253024-b393ea2u	313	6	in	in	IN
cord-253024-b393ea2u	313	7	all	all	DT
cord-253024-b393ea2u	313	8	panels	panel	NNS
cord-253024-b393ea2u	313	9	.	.	.
cord-253024-b393ea2u	314	1	polymerase	polymerase	NNP
cord-253024-b393ea2u	314	2	region	region	NN
cord-253024-b393ea2u	314	3	of	of	IN
cord-253024-b393ea2u	314	4	MHV	MHV	NNP
cord-253024-b393ea2u	314	5	-	-	HYPH
cord-253024-b393ea2u	314	6	A59	A59	NNP
cord-253024-b393ea2u	314	7	may	may	MD
cord-253024-b393ea2u	314	8	be	be	VB
cord-253024-b393ea2u	314	9	grossly	grossly	RB
cord-253024-b393ea2u	314	10	under	under	IN
cord-253024-b393ea2u	314	11	-	-	HYPH
cord-253024-b393ea2u	314	12	LA6	la6	NN
cord-253024-b393ea2u	314	13	)	)	-RRB-
cord-253024-b393ea2u	314	14	was	be	VBD
cord-253024-b393ea2u	314	15	unclear	unclear	JJ
cord-253024-b393ea2u	314	16	.	.	.
cord-253024-b393ea2u	315	1	This	this	DT
cord-253024-b393ea2u	315	2	seems	seem	VBZ
cord-253024-b393ea2u	315	3	unlikely	unlikely	JJ
cord-253024-b393ea2u	315	4	because	because	IN
cord-253024-b393ea2u	315	5	a	a	DT
cord-253024-b393ea2u	315	6	uniestimated	uniestimate	VBN
cord-253024-b393ea2u	315	7	because	because	IN
cord-253024-b393ea2u	315	8	the	the	DT
cord-253024-b393ea2u	315	9	exact	exact	JJ
cord-253024-b393ea2u	315	10	location	location	NN
cord-253024-b393ea2u	315	11	of	of	IN
cord-253024-b393ea2u	315	12	the	the	DT
cord-253024-b393ea2u	315	13	ts	ts	NNP
cord-253024-b393ea2u	315	14	allele	allele	NN
cord-253024-b393ea2u	315	15	in	in	IN
cord-253024-b393ea2u	315	16	form	form	NN
cord-253024-b393ea2u	315	17	recombination	recombination	NN
cord-253024-b393ea2u	315	18	frequency	frequency	NN
cord-253024-b393ea2u	315	19	of	of	IN
cord-253024-b393ea2u	315	20	lo	lo	NNP
cord-253024-b393ea2u	315	21	/	/	SYM
cord-253024-b393ea2u	315	22	o/575	o/575	NN
cord-253024-b393ea2u	315	23	nucleotides	nucleotide	NNS
cord-253024-b393ea2u	315	24	the	the	DT
cord-253024-b393ea2u	315	25	group	group	NN
cord-253024-b393ea2u	315	26	E	e	NN
cord-253024-b393ea2u	315	27	mutant	mutant	NN
cord-253024-b393ea2u	315	28	(	(	-LRB-
cord-253024-b393ea2u	315	29	LA1	LA1	NNP
cord-253024-b393ea2u	315	30	8)	8)	NNP
cord-253024-b393ea2u	315	31	and	and	CC
cord-253024-b393ea2u	315	32	group	group	NN
cord-253024-b393ea2u	315	33	A	a	DT
cord-253024-b393ea2u	315	34	mutants	mutant	NNS
cord-253024-b393ea2u	315	35	(	(	-LRB-
cord-253024-b393ea2u	315	36	LA3/	LA3/	NNP
cord-253024-b393ea2u	315	37	of	of	IN
cord-253024-b393ea2u	315	38	dsRNA	dsrna	NN
cord-253024-b393ea2u	315	39	throughout	throughout	IN
cord-253024-b393ea2u	315	40	the	the	DT
cord-253024-b393ea2u	315	41	genome	genome	NN
cord-253024-b393ea2u	316	1	would	would	MD
cord-253024-b393ea2u	316	2	:	:	:
cord-253024-b393ea2u	316	3	(	(	-LRB-
cord-253024-b393ea2u	316	4	1	1	LS
cord-253024-b393ea2u	316	5	)	)	-RRB-
cord-253024-b393ea2u	316	6	predict	predict	VB
cord-253024-b393ea2u	316	7	the	the	DT
cord-253024-b393ea2u	316	8	recombination	recombination	NN
cord-253024-b393ea2u	316	9	frequency	frequency	NN
cord-253024-b393ea2u	316	10	for	for	IN
cord-253024-b393ea2u	316	11	the	the	DT
cord-253024-b393ea2u	316	12	MI	MI	NNP
cord-253024-b393ea2u	316	13	-	-	HYPH
cord-253024-b393ea2u	316	14	IV	IV	NNP
cord-253024-b393ea2u	316	15	genome	genome	NN
cord-253024-b393ea2u	316	16	to	to	TO
cord-253024-b393ea2u	316	17	approach	approach	VB
cord-253024-b393ea2u	316	18	-56	-56	IN
cord-253024-b393ea2u	316	19	%	%	NN
cord-253024-b393ea2u	316	20	or	or	CC
cord-253024-b393ea2u	316	21	more	more	JJR
cord-253024-b393ea2u	316	22	;	;	:
cord-253024-b393ea2u	316	23	(	(	-LRB-
cord-253024-b393ea2u	316	24	2	2	LS
cord-253024-b393ea2u	316	25	)	)	-RRB-
cord-253024-b393ea2u	316	26	place	place	NN
cord-253024-b393ea2u	317	1	RNA	rna	NN
cord-253024-b393ea2u	317	2	-	-	HYPH
cord-253024-b393ea2u	317	3	complementation	complementation	NN
cord-253024-b393ea2u	317	4	groups	group	NNS
cord-253024-b393ea2u	317	5	A	A	NNP
cord-253024-b393ea2u	317	6	,	,	,
cord-253024-b393ea2u	317	7	B	b	NN
cord-253024-b393ea2u	317	8	,	,	,
cord-253024-b393ea2u	317	9	and	and	CC
cord-253024-b393ea2u	317	10	C	C	NNP
cord-253024-b393ea2u	317	11	in	in	IN
cord-253024-b393ea2u	317	12	ORF	ORF	NNP
cord-253024-b393ea2u	317	13	1	1	CD
cord-253024-b393ea2u	317	14	b	b	NNP
cord-253024-b393ea2u	317	15	and	and	CC
cord-253024-b393ea2u	317	16	RNA	RNA	NNP
cord-253024-b393ea2u	317	17	-	-	HYPH
cord-253024-b393ea2u	317	18	complementation	complementation	NN
cord-253024-b393ea2u	317	19	groups	group	NNS
cord-253024-b393ea2u	317	20	D	d	NN
cord-253024-b393ea2u	317	21	and	and	CC
cord-253024-b393ea2u	317	22	E	e	NN
cord-253024-b393ea2u	317	23	in	in	IN
cord-253024-b393ea2u	317	24	the	the	DT
cord-253024-b393ea2u	317	25	p30	p30	NN
cord-253024-b393ea2u	317	26	/HE	/HE	HYPH
cord-253024-b393ea2u	317	27	nonstructural	nonstructural	JJ
cord-253024-b393ea2u	317	28	proteins	protein	NNS
cord-253024-b393ea2u	317	29	,	,	,
cord-253024-b393ea2u	317	30	and	and	CC
cord-253024-b393ea2u	317	31	(	(	-LRB-
cord-253024-b393ea2u	317	32	3	3	LS
cord-253024-b393ea2u	317	33	)	)	-RRB-
cord-253024-b393ea2u	317	34	suggest	suggest	VB
cord-253024-b393ea2u	317	35	that	that	IN
cord-253024-b393ea2u	317	36	conditional	conditional	JJ
cord-253024-b393ea2u	317	37	lethal	lethal	JJ
cord-253024-b393ea2u	317	38	mutants	mutant	NNS
cord-253024-b393ea2u	317	39	were	be	VBD
cord-253024-b393ea2u	317	40	not	not	RB
cord-253024-b393ea2u	317	41	isolated	isolate	VBN
cord-253024-b393ea2u	317	42	within	within	IN
cord-253024-b393ea2u	317	43	ORFla	ORFla	NNP
cord-253024-b393ea2u	317	44	at	at	IN
cord-253024-b393ea2u	317	45	the	the	DT
cord-253024-b393ea2u	317	46	5'most	5'most	CD
cord-253024-b393ea2u	317	47	three	three	CD
cord-253024-b393ea2u	317	48	-	-	HYPH
cord-253024-b393ea2u	317	49	fifths	fifth	NNS
cord-253024-b393ea2u	317	50	(	(	-LRB-
cord-253024-b393ea2u	317	51	-14	-14	NNP
cord-253024-b393ea2u	317	52	kb	kb	NNP
cord-253024-b393ea2u	317	53	)	)	-RRB-
cord-253024-b393ea2u	317	54	of	of	IN
cord-253024-b393ea2u	317	55	the	the	DT
cord-253024-b393ea2u	317	56	genome	genome	NN
cord-253024-b393ea2u	317	57	.	.	.
cord-253024-b393ea2u	318	1	This	this	DT
cord-253024-b393ea2u	318	2	is	be	VBZ
cord-253024-b393ea2u	318	3	highly	highly	RB
cord-253024-b393ea2u	318	4	unlikely	unlikely	JJ
cord-253024-b393ea2u	318	5	since	since	IN
cord-253024-b393ea2u	318	6	several	several	JJ
cord-253024-b393ea2u	318	7	proteolytic	proteolytic	JJ
cord-253024-b393ea2u	318	8	,	,	,
cord-253024-b393ea2u	318	9	hydrophobic	hydrophobic	JJ
cord-253024-b393ea2u	318	10	membrane	membrane	NN
cord-253024-b393ea2u	318	11	-	-	HYPH
cord-253024-b393ea2u	318	12	anchoring	anchor	VBG
cord-253024-b393ea2u	318	13	and	and	CC
cord-253024-b393ea2u	318	14	cysteine	cysteine	NN
cord-253024-b393ea2u	318	15	-	-	HYPH
cord-253024-b393ea2u	318	16	rich	rich	JJ
cord-253024-b393ea2u	318	17	domains	domain	NNS
cord-253024-b393ea2u	318	18	have	have	VBP
cord-253024-b393ea2u	318	19	been	be	VBN
cord-253024-b393ea2u	318	20	identified	identify	VBN
cord-253024-b393ea2u	318	21	in	in	IN
cord-253024-b393ea2u	318	22	ORF	ORF	NNP
cord-253024-b393ea2u	318	23	1	1	CD
cord-253024-b393ea2u	318	24	a	a	NNP
cord-253024-b393ea2u	318	25	,	,	,
cord-253024-b393ea2u	318	26	which	which	WDT
cord-253024-b393ea2u	318	27	should	should	MD
cord-253024-b393ea2u	318	28	be	be	VB
cord-253024-b393ea2u	318	29	amenable	amenable	JJ
cord-253024-b393ea2u	318	30	to	to	IN
cord-253024-b393ea2u	318	31	mutagenesis	mutagenesis	NN
cord-253024-b393ea2u	318	32	(	(	-LRB-
cord-253024-b393ea2u	318	33	Baker	Baker	NNP
cord-253024-b393ea2u	318	34	et	et	NNP
cord-253024-b393ea2u	318	35	a/.	a/.	NNP
cord-253024-b393ea2u	318	36	,	,	,
cord-253024-b393ea2u	318	37	1989	1989	CD
cord-253024-b393ea2u	318	38	;	;	:
cord-253024-b393ea2u	318	39	Lee	Lee	NNP
cord-253024-b393ea2u	318	40	et	et	NNP
cord-253024-b393ea2u	318	41	a/.	a/.	NNP
cord-253024-b393ea2u	318	42	,	,	,
cord-253024-b393ea2u	318	43	1991	1991	CD
cord-253024-b393ea2u	318	44	)	)	-RRB-
cord-253024-b393ea2u	318	45	.	.	.
cord-253024-b393ea2u	319	1	More	more	RBR
cord-253024-b393ea2u	319	2	importantly	importantly	RB
cord-253024-b393ea2u	319	3	,	,	,
cord-253024-b393ea2u	319	4	Tl	Tl	NNP
cord-253024-b393ea2u	319	5	fingerprint	fingerprint	NN
cord-253024-b393ea2u	319	6	analysis	analysis	NN
cord-253024-b393ea2u	319	7	of	of	IN
cord-253024-b393ea2u	319	8	RNA	RNA	NNP
cord-253024-b393ea2u	319	9	recombinant	recombinant	JJ
cord-253024-b393ea2u	319	10	viruses	virus	NNS
cord-253024-b393ea2u	319	11	derived	derive	VBN
cord-253024-b393ea2u	319	12	from	from	IN
cord-253024-b393ea2u	319	13	ts	ts	JJ
cord-253024-b393ea2u	319	14	mutants	mutant	NNS
cord-253024-b393ea2u	319	15	in	in	IN
cord-253024-b393ea2u	319	16	complementation	complementation	NN
cord-253024-b393ea2u	319	17	groups	group	NNS
cord-253024-b393ea2u	319	18	C	C	NNP
cord-253024-b393ea2u	319	19	,	,	,
cord-253024-b393ea2u	319	20	D	D	NNP
cord-253024-b393ea2u	319	21	,	,	,
cord-253024-b393ea2u	319	22	and	and	CC
cord-253024-b393ea2u	319	23	E	e	LS
cord-253024-b393ea2u	319	24	clearly	clearly	RB
cord-253024-b393ea2u	319	25	place	place	VBP
cord-253024-b393ea2u	319	26	each	each	DT
cord-253024-b393ea2u	319	27	genetic	genetic	JJ
cord-253024-b393ea2u	319	28	function	function	NN
cord-253024-b393ea2u	319	29	in	in	IN
cord-253024-b393ea2u	319	30	the	the	DT
cord-253024-b393ea2u	319	31	polymerase	polymerase	NN
cord-253024-b393ea2u	319	32	gene	gene	NN
cord-253024-b393ea2u	319	33	(	(	-LRB-
cord-253024-b393ea2u	319	34	Keck	Keck	NNP
cord-253024-b393ea2u	319	35	et	et	NNP
cord-253024-b393ea2u	319	36	a/.	a/.	NNP
cord-253024-b393ea2u	319	37	,	,	,
cord-253024-b393ea2u	319	38	1987	1987	CD
cord-253024-b393ea2u	319	39	;	;	:
cord-253024-b393ea2u	319	40	Lai	Lai	NNP
cord-253024-b393ea2u	319	41	et	et	FW
cord-253024-b393ea2u	319	42	al	al	NNP
cord-253024-b393ea2u	319	43	.	.	NNP
cord-253024-b393ea2u	319	44	,	,	,
cord-253024-b393ea2u	319	45	1985	1985	CD
cord-253024-b393ea2u	319	46	)	)	-RRB-
cord-253024-b393ea2u	319	47	.	.	.
cord-253024-b393ea2u	320	1	Since	since	IN
cord-253024-b393ea2u	320	2	RNA	rna	NN
cord-253024-b393ea2u	320	3	-	-	HYPH
cord-253024-b393ea2u	320	4	complementation	complementation	NN
cord-253024-b393ea2u	320	5	groups	group	NNS
cord-253024-b393ea2u	320	6	C	c	NN
cord-253024-b393ea2u	320	7	,	,	,
cord-253024-b393ea2u	320	8	D	D	NNP
cord-253024-b393ea2u	320	9	,	,	,
cord-253024-b393ea2u	320	10	and	and	CC
cord-253024-b393ea2u	320	11	E	E	NNS
cord-253024-b393ea2u	320	12	have	have	VBP
cord-253024-b393ea2u	320	13	also	also	RB
cord-253024-b393ea2u	320	14	been	be	VBN
cord-253024-b393ea2u	320	15	demonstrated	demonstrate	VBN
cord-253024-b393ea2u	320	16	to	to	TO
cord-253024-b393ea2u	320	17	function	function	VB
cord-253024-b393ea2u	320	18	in	in	IN
cord-253024-b393ea2u	320	19	mRNA	mRNA	NNP
cord-253024-b393ea2u	320	20	synthesis	synthesis	NN
cord-253024-b393ea2u	320	21	and	and	CC
cord-253024-b393ea2u	320	22	map	map	NN
cord-253024-b393ea2u	320	23	within	within	IN
cord-253024-b393ea2u	320	24	ORF	ORF	NNP
cord-253024-b393ea2u	320	25	1	1	CD
cord-253024-b393ea2u	320	26	b	b	NNP
cord-253024-b393ea2u	320	27	,	,	,
cord-253024-b393ea2u	320	28	which	which	WDT
cord-253024-b393ea2u	320	29	contains	contain	VBZ
cord-253024-b393ea2u	320	30	polymerase	polymerase	NN
cord-253024-b393ea2u	320	31	,	,	,
cord-253024-b393ea2u	320	32	helicase	helicase	NN
cord-253024-b393ea2u	320	33	,	,	,
cord-253024-b393ea2u	320	34	and	and	CC
cord-253024-b393ea2u	320	35	metal	metal	NN
cord-253024-b393ea2u	320	36	-	-	HYPH
cord-253024-b393ea2u	320	37	binding	bind	VBG
cord-253024-b393ea2u	320	38	sequence	sequence	NN
cord-253024-b393ea2u	320	39	motifs	motifs	NN
cord-253024-b393ea2u	320	40	,	,	,
cord-253024-b393ea2u	320	41	it	-PRON-	PRP
cord-253024-b393ea2u	320	42	is	be	VBZ
cord-253024-b393ea2u	320	43	extremely	extremely	RB
cord-253024-b393ea2u	320	44	unlikely	unlikely	JJ
cord-253024-b393ea2u	320	45	that	that	IN
cord-253024-b393ea2u	320	46	these	these	DT
cord-253024-b393ea2u	320	47	mutants	mutant	NNS
cord-253024-b393ea2u	320	48	map	map	VBP
cord-253024-b393ea2u	320	49	in	in	IN
cord-253024-b393ea2u	320	50	the	the	DT
cord-253024-b393ea2u	320	51	HE	HE	NNP
cord-253024-b393ea2u	320	52	or	or	CC
cord-253024-b393ea2u	320	53	p30	p30	NNP
cord-253024-b393ea2u	320	54	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	320	55	ORFs	ORFs	NNP
cord-253024-b393ea2u	320	56	Bredenbeck	Bredenbeck	NNP
cord-253024-b393ea2u	320	57	et	et	NNP
cord-253024-b393ea2u	320	58	a/.	a/.	NN
cord-253024-b393ea2u	320	59	,	,	,
cord-253024-b393ea2u	320	60	1990	1990	CD
cord-253024-b393ea2u	320	61	;	;	:
cord-253024-b393ea2u	320	62	Lee	Lee	NNP
cord-253024-b393ea2u	320	63	et	et	NNP
cord-253024-b393ea2u	320	64	al	al	NNP
cord-253024-b393ea2u	320	65	.	.	NNP
cord-253024-b393ea2u	320	66	,	,	,
cord-253024-b393ea2u	320	67	1991	1991	CD
cord-253024-b393ea2u	320	68	;	;	:
cord-253024-b393ea2u	321	1	Schaad	Schaad	NNP
cord-253024-b393ea2u	321	2	et	et	NNP
cord-253024-b393ea2u	321	3	al	al	NNP
cord-253024-b393ea2u	321	4	.	.	NNP
cord-253024-b393ea2u	321	5	,	,	,
cord-253024-b393ea2u	321	6	1990	1990	CD
cord-253024-b393ea2u	321	7	)	)	-RRB-
cord-253024-b393ea2u	321	8	.	.	.
cord-253024-b393ea2u	322	1	In	in	IN
cord-253024-b393ea2u	322	2	contrast	contrast	NN
cord-253024-b393ea2u	322	3	,	,	,
cord-253024-b393ea2u	322	4	the	the	DT
cord-253024-b393ea2u	322	5	recombination	recombination	NN
cord-253024-b393ea2u	322	6	rate	rate	NN
cord-253024-b393ea2u	322	7	predicted	predict	VBN
cord-253024-b393ea2u	322	8	through	through	IN
cord-253024-b393ea2u	322	9	the	the	DT
cord-253024-b393ea2u	322	10	polymerase	polymerase	NN
cord-253024-b393ea2u	322	11	region	region	NN
cord-253024-b393ea2u	322	12	was	be	VBD
cord-253024-b393ea2u	322	13	not	not	RB
cord-253024-b393ea2u	322	14	inordinately	inordinately	RB
cord-253024-b393ea2u	322	15	high	high	JJ
cord-253024-b393ea2u	322	16	(	(	-LRB-
cord-253024-b393ea2u	322	17	1%/1800	1%/1800	CD
cord-253024-b393ea2u	322	18	-	-	SYM
cord-253024-b393ea2u	322	19	2500	2500	CD
cord-253024-b393ea2u	322	20	bp	bp	NNP
cord-253024-b393ea2u	322	21	of	of	IN
cord-253024-b393ea2u	322	22	dsRNA	dsRNA	NNP
cord-253024-b393ea2u	322	23	)	)	-RRB-
cord-253024-b393ea2u	322	24	,	,	,
cord-253024-b393ea2u	322	25	but	but	CC
cord-253024-b393ea2u	322	26	rather	rather	RB
cord-253024-b393ea2u	322	27	closely	closely	RB
cord-253024-b393ea2u	322	28	approximated	approximate	VBD
cord-253024-b393ea2u	322	29	the	the	DT
cord-253024-b393ea2u	322	30	recombination	recombination	NN
cord-253024-b393ea2u	322	31	frequencies	frequency	NNS
cord-253024-b393ea2u	322	32	estimated	estimate	VBN
cord-253024-b393ea2u	322	33	to	to	TO
cord-253024-b393ea2u	322	34	occur	occur	VB
cord-253024-b393ea2u	322	35	in	in	IN
cord-253024-b393ea2u	322	36	apthovirus	apthovirus	NNP
cord-253024-b393ea2u	322	37	and	and	CC
cord-253024-b393ea2u	322	38	poliovirus	poliovirus	NN
cord-253024-b393ea2u	322	39	infections	infection	NNS
cord-253024-b393ea2u	322	40	(	(	-LRB-
cord-253024-b393ea2u	322	41	Cooper	Cooper	NNP
cord-253024-b393ea2u	322	42	,	,	,
cord-253024-b393ea2u	322	43	1977	1977	CD
cord-253024-b393ea2u	322	44	;	;	:
cord-253024-b393ea2u	322	45	King	King	NNP
cord-253024-b393ea2u	322	46	,	,	,
cord-253024-b393ea2u	322	47	1987	1987	CD
cord-253024-b393ea2u	322	48	)	)	-RRB-
cord-253024-b393ea2u	322	49	.	.	.
cord-253024-b393ea2u	323	1	Thus	thus	RB
cord-253024-b393ea2u	323	2	,	,	,
cord-253024-b393ea2u	323	3	it	-PRON-	PRP
cord-253024-b393ea2u	323	4	seems	seem	VBZ
cord-253024-b393ea2u	323	5	likely	likely	JJ
cord-253024-b393ea2u	323	6	that	that	IN
cord-253024-b393ea2u	323	7	the	the	DT
cord-253024-b393ea2u	323	8	frequencies	frequency	NNS
cord-253024-b393ea2u	323	9	measured	measure	VBN
cord-253024-b393ea2u	323	10	between	between	IN
cord-253024-b393ea2u	323	11	the	the	DT
cord-253024-b393ea2u	323	12	polymerase	polymerase	NN
cord-253024-b393ea2u	323	13	and	and	CC
cord-253024-b393ea2u	323	14	S	S	NNP
cord-253024-b393ea2u	323	15	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	323	16	genes	gene	NNS
cord-253024-b393ea2u	323	17	actually	actually	RB
cord-253024-b393ea2u	323	18	represent	represent	VBP
cord-253024-b393ea2u	323	19	true	true	JJ
cord-253024-b393ea2u	323	20	differences	difference	NNS
cord-253024-b393ea2u	323	21	in	in	IN
cord-253024-b393ea2u	323	22	the	the	DT
cord-253024-b393ea2u	323	23	recombination	recombination	NN
cord-253024-b393ea2u	323	24	rate	rate	NN
cord-253024-b393ea2u	323	25	.	.	.
cord-253024-b393ea2u	324	1	Since	since	IN
cord-253024-b393ea2u	324	2	the	the	DT
cord-253024-b393ea2u	324	3	enhanced	enhance	VBN
cord-253024-b393ea2u	324	4	recombination	recombination	NN
cord-253024-b393ea2u	324	5	frequencies	frequency	NNS
cord-253024-b393ea2u	324	6	in	in	IN
cord-253024-b393ea2u	324	7	MHV	MHV	NNP
cord-253024-b393ea2u	324	8	were	be	VBD
cord-253024-b393ea2u	324	9	observed	observe	VBN
cord-253024-b393ea2u	324	10	within	within	IN
cord-253024-b393ea2u	324	11	a	a	DT
cord-253024-b393ea2u	324	12	physically	physically	RB
cord-253024-b393ea2u	324	13	smaller	small	JJR
cord-253024-b393ea2u	324	14	(	(	-LRB-
cord-253024-b393ea2u	324	15	4.0-kb	4.0-kb	CD
cord-253024-b393ea2u	324	16	)	)	-RRB-
cord-253024-b393ea2u	324	17	region	region	NN
cord-253024-b393ea2u	324	18	as	as	IN
cord-253024-b393ea2u	324	19	compared	compare	VBN
cord-253024-b393ea2u	324	20	to	to	IN
cord-253024-b393ea2u	324	21	the	the	DT
cord-253024-b393ea2u	324	22	22-kb	22-kb	CD
cord-253024-b393ea2u	324	23	polymerase	polymerase	NN
cord-253024-b393ea2u	324	24	region	region	NN
cord-253024-b393ea2u	324	25	,	,	,
cord-253024-b393ea2u	324	26	it	-PRON-	PRP
cord-253024-b393ea2u	324	27	is	be	VBZ
cord-253024-b393ea2u	324	28	possible	possible	JJ
cord-253024-b393ea2u	324	29	that	that	IN
cord-253024-b393ea2u	324	30	a	a	DT
cord-253024-b393ea2u	324	31	mechanism	mechanism	NN
cord-253024-b393ea2u	324	32	similar	similar	JJ
cord-253024-b393ea2u	324	33	to	to	IN
cord-253024-b393ea2u	324	34	high	high	JJ
cord-253024-b393ea2u	324	35	negative	negative	JJ
cord-253024-b393ea2u	324	36	interference	interference	NN
cord-253024-b393ea2u	324	37	in	in	IN
cord-253024-b393ea2u	324	38	DNA	dna	NN
cord-253024-b393ea2u	324	39	viruses	virus	NNS
cord-253024-b393ea2u	324	40	may	may	MD
cord-253024-b393ea2u	324	41	account	account	VB
cord-253024-b393ea2u	324	42	for	for	IN
cord-253024-b393ea2u	324	43	the	the	DT
cord-253024-b393ea2u	324	44	increased	increase	VBN
cord-253024-b393ea2u	324	45	rates	rate	NNS
cord-253024-b393ea2u	324	46	of	of	IN
cord-253024-b393ea2u	324	47	recombination	recombination	NN
cord-253024-b393ea2u	324	48	(	(	-LRB-
cord-253024-b393ea2u	324	49	Chase	Chase	NNP
cord-253024-b393ea2u	324	50	and	and	CC
cord-253024-b393ea2u	324	51	Doermann	Doermann	NNP
cord-253024-b393ea2u	324	52	,	,	,
cord-253024-b393ea2u	324	53	1958	1958	CD
cord-253024-b393ea2u	324	54	)	)	-RRB-
cord-253024-b393ea2u	324	55	.	.	.
cord-253024-b393ea2u	325	1	This	this	DT
cord-253024-b393ea2u	325	2	seems	seem	VBZ
cord-253024-b393ea2u	325	3	unlikely	unlikely	JJ
cord-253024-b393ea2u	325	4	since	since	IN
cord-253024-b393ea2u	325	5	high	high	JJ
cord-253024-b393ea2u	325	6	negative	negative	JJ
cord-253024-b393ea2u	325	7	interference	interference	NN
cord-253024-b393ea2u	325	8	is	be	VBZ
cord-253024-b393ea2u	325	9	probably	probably	RB
cord-253024-b393ea2u	325	10	mediated	mediate	VBN
cord-253024-b393ea2u	325	11	by	by	IN
cord-253024-b393ea2u	325	12	DNA	dna	NN
cord-253024-b393ea2u	325	13	polymerase	polymerase	NN
cord-253024-b393ea2u	325	14	repair	repair	NN
cord-253024-b393ea2u	325	15	mechanisms	mechanism	NNS
cord-253024-b393ea2u	325	16	,	,	,
cord-253024-b393ea2u	325	17	which	which	WDT
cord-253024-b393ea2u	325	18	have	have	VBP
cord-253024-b393ea2u	325	19	not	not	RB
cord-253024-b393ea2u	325	20	been	be	VBN
cord-253024-b393ea2u	325	21	demonstrated	demonstrate	VBN
cord-253024-b393ea2u	325	22	in	in	IN
cord-253024-b393ea2u	325	23	the	the	DT
cord-253024-b393ea2u	325	24	MHV	MHV	NNP
cord-253024-b393ea2u	325	25	RNA	RNA	NNP
cord-253024-b393ea2u	325	26	polymerase	polymerase	NN
cord-253024-b393ea2u	325	27	(	(	-LRB-
cord-253024-b393ea2u	325	28	Glickman	Glickman	NNP
cord-253024-b393ea2u	325	29	and	and	CC
cord-253024-b393ea2u	325	30	Radman	Radman	NNP
cord-253024-b393ea2u	325	31	,	,	,
cord-253024-b393ea2u	325	32	1980	1980	CD
cord-253024-b393ea2u	325	33	)	)	-RRB-
cord-253024-b393ea2u	325	34	.	.	.
cord-253024-b393ea2u	326	1	The	the	DT
cord-253024-b393ea2u	326	2	increase	increase	NN
cord-253024-b393ea2u	326	3	in	in	IN
cord-253024-b393ea2u	326	4	the	the	DT
cord-253024-b393ea2u	326	5	recombination	recombination	NN
cord-253024-b393ea2u	326	6	rate	rate	NN
cord-253024-b393ea2u	326	7	in	in	IN
cord-253024-b393ea2u	326	8	the	the	DT
cord-253024-b393ea2u	326	9	S	S	NNP
cord-253024-b393ea2u	326	10	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	326	11	gene	gene	NN
cord-253024-b393ea2u	326	12	could	could	MD
cord-253024-b393ea2u	326	13	also	also	RB
cord-253024-b393ea2u	326	14	not	not	RB
cord-253024-b393ea2u	326	15	be	be	VB
cord-253024-b393ea2u	326	16	attributed	attribute	VBN
cord-253024-b393ea2u	326	17	to	to	IN
cord-253024-b393ea2u	326	18	a	a	DT
cord-253024-b393ea2u	326	19	combination	combination	NN
cord-253024-b393ea2u	326	20	of	of	IN
cord-253024-b393ea2u	326	21	true	true	JJ
cord-253024-b393ea2u	326	22	homologous	homologous	JJ
cord-253024-b393ea2u	326	23	recombination	recombination	NN
cord-253024-b393ea2u	326	24	and	and	CC
cord-253024-b393ea2u	326	25	recombination	recombination	NN
cord-253024-b393ea2u	326	26	resulting	result	VBG
cord-253024-b393ea2u	326	27	in	in	IN
cord-253024-b393ea2u	326	28	large	large	JJ
cord-253024-b393ea2u	326	29	deletions	deletion	NNS
cord-253024-b393ea2u	326	30	.	.	.
cord-253024-b393ea2u	327	1	Nonhomologous	Nonhomologous	NNP
cord-253024-b393ea2u	327	2	recombination	recombination	NN
cord-253024-b393ea2u	327	3	probably	probably	RB
cord-253024-b393ea2u	327	4	explains	explain	VBZ
cord-253024-b393ea2u	327	5	the	the	DT
cord-253024-b393ea2u	327	6	appearance	appearance	NN
cord-253024-b393ea2u	327	7	of	of	IN
cord-253024-b393ea2u	327	8	defective	defective	JJ
cord-253024-b393ea2u	327	9	interfering	interfere	VBG
cord-253024-b393ea2u	327	10	RNAs	rna	NNS
cord-253024-b393ea2u	327	11	of	of	IN
cord-253024-b393ea2u	327	12	Sindbis	Sindbis	NNP
cord-253024-b393ea2u	327	13	virus	virus	NN
cord-253024-b393ea2u	327	14	,	,	,
cord-253024-b393ea2u	327	15	vesicular	vesicular	JJ
cord-253024-b393ea2u	327	16	stomatitis	stomatitis	NN
cord-253024-b393ea2u	327	17	virus	virus	NN
cord-253024-b393ea2u	327	18	,	,	,
cord-253024-b393ea2u	327	19	and	and	CC
cord-253024-b393ea2u	327	20	MHV	MHV	NNP
cord-253024-b393ea2u	327	21	(	(	-LRB-
cord-253024-b393ea2u	327	22	Holland	Holland	NNP
cord-253024-b393ea2u	327	23	,	,	,
cord-253024-b393ea2u	327	24	1987	1987	CD
cord-253024-b393ea2u	327	25	;	;	:
cord-253024-b393ea2u	327	26	Makino	Makino	NNP
cord-253024-b393ea2u	327	27	et	et	NNP
cord-253024-b393ea2u	327	28	a/.	a/.	NN
cord-253024-b393ea2u	327	29	,	,	,
cord-253024-b393ea2u	327	30	1988	1988	CD
cord-253024-b393ea2u	327	31	Monroe	Monroe	NNP
cord-253024-b393ea2u	327	32	and	and	CC
cord-253024-b393ea2u	327	33	Schlesinger	Schlesinger	NNP
cord-253024-b393ea2u	327	34	,	,	,
cord-253024-b393ea2u	327	35	1983	1983	CD
cord-253024-b393ea2u	327	36	)	)	-RRB-
cord-253024-b393ea2u	327	37	.	.	.
cord-253024-b393ea2u	328	1	Since	since	IN
cord-253024-b393ea2u	328	2	deletions	deletion	NNS
cord-253024-b393ea2u	328	3	have	have	VBP
cord-253024-b393ea2u	328	4	been	be	VBN
cord-253024-b393ea2u	328	5	demonstrated	demonstrate	VBN
cord-253024-b393ea2u	328	6	in	in	IN
cord-253024-b393ea2u	328	7	the	the	DT
cord-253024-b393ea2u	328	8	MHV	MHV	NNP
cord-253024-b393ea2u	328	9	S	S	NNP
cord-253024-b393ea2u	328	10	and	and	CC
cord-253024-b393ea2u	328	11	HE	HE	NNP
cord-253024-b393ea2u	328	12	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	328	13	genes	gene	NNS
cord-253024-b393ea2u	328	14	as	as	RB
cord-253024-b393ea2u	328	15	well	well	RB
cord-253024-b393ea2u	328	16	as	as	IN
cord-253024-b393ea2u	328	17	in	in	IN
cord-253024-b393ea2u	328	18	the	the	DT
cord-253024-b393ea2u	328	19	nonstructural	nonstructural	JJ
cord-253024-b393ea2u	328	20	genes	gene	NNS
cord-253024-b393ea2u	328	21	encoded	encode	VBN
cord-253024-b393ea2u	328	22	in	in	IN
cord-253024-b393ea2u	328	23	mRNA	mRNA	NNP
cord-253024-b393ea2u	328	24	4	4	CD
cord-253024-b393ea2u	328	25	and	and	CC
cord-253024-b393ea2u	328	26	5	5	CD
cord-253024-b393ea2u	328	27	and	and	CC
cord-253024-b393ea2u	328	28	in	in	IN
cord-253024-b393ea2u	328	29	the	the	DT
cord-253024-b393ea2u	328	30	TGEV	TGEV	NNP
cord-253024-b393ea2u	328	31	S	S	NNP
cord-253024-b393ea2u	328	32	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	328	33	gene	gene	NN
cord-253024-b393ea2u	328	34	and	and	CC
cord-253024-b393ea2u	328	35	other	other	JJ
cord-253024-b393ea2u	328	36	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	328	37	ORFs	ORFs	NNP
cord-253024-b393ea2u	329	1	(	(	-LRB-
cord-253024-b393ea2u	329	2	Ban	Ban	NNP
cord-253024-b393ea2u	329	3	-	-	HYPH
cord-253024-b393ea2u	329	4	ner	ner	NNP
cord-253024-b393ea2u	329	5	el	el	NNP
cord-253024-b393ea2u	329	6	al	al	NNP
cord-253024-b393ea2u	329	7	.	.	.
cord-253024-b393ea2u	330	1	,	,	,
cord-253024-b393ea2u	330	2	1990	1990	CD
cord-253024-b393ea2u	330	3	;	;	:
cord-253024-b393ea2u	330	4	La	La	NNP
cord-253024-b393ea2u	330	5	Monica	Monica	NNP
cord-253024-b393ea2u	330	6	et	et	NNP
cord-253024-b393ea2u	330	7	al	al	NNP
cord-253024-b393ea2u	330	8	.	.	.
cord-253024-b393ea2u	331	1	,	,	,
cord-253024-b393ea2u	331	2	1991	1991	CD
cord-253024-b393ea2u	331	3	;	;	:
cord-253024-b393ea2u	331	4	Luytjes	Luytjes	NNP
cord-253024-b393ea2u	331	5	et	et	NNP
cord-253024-b393ea2u	331	6	al	al	NNP
cord-253024-b393ea2u	331	7	.	.	NNP
cord-253024-b393ea2u	331	8	,	,	,
cord-253024-b393ea2u	331	9	1987	1987	CD
cord-253024-b393ea2u	331	10	;	;	:
cord-253024-b393ea2u	331	11	Parker	Parker	NNP
cord-253024-b393ea2u	331	12	et	et	NNP
cord-253024-b393ea2u	331	13	al	al	NNP
cord-253024-b393ea2u	331	14	.	.	NNP
cord-253024-b393ea2u	331	15	,	,	,
cord-253024-b393ea2u	331	16	1990	1990	CD
cord-253024-b393ea2u	332	1	;	;	:
cord-253024-b393ea2u	332	2	Rasschaert	Rasschaert	NNP
cord-253024-b393ea2u	332	3	et	et	NNP
cord-253024-b393ea2u	332	4	al	al	NNP
cord-253024-b393ea2u	332	5	.	.	NNP
cord-253024-b393ea2u	332	6	,	,	,
cord-253024-b393ea2u	333	1	I	-PRON-	PRP
cord-253024-b393ea2u	333	2	990	990	CD
cord-253024-b393ea2u	333	3	;	;	:
cord-253024-b393ea2u	333	4	Schwartz	Schwartz	NNP
cord-253024-b393ea2u	333	5	et	et	NNP
cord-253024-b393ea2u	333	6	al	al	NNP
cord-253024-b393ea2u	333	7	.	.	NNP
cord-253024-b393ea2u	333	8	,	,	,
cord-253024-b393ea2u	333	9	1990	1990	CD
cord-253024-b393ea2u	333	10	;	;	:
cord-253024-b393ea2u	333	11	Yokomori	Yokomori	NNP
cord-253024-b393ea2u	333	12	and	and	CC
cord-253024-b393ea2u	333	13	Lai	Lai	NNP
cord-253024-b393ea2u	333	14	,	,	,
cord-253024-b393ea2u	333	15	1991	1991	CD
cord-253024-b393ea2u	333	16	)	)	-RRB-
cord-253024-b393ea2u	333	17	,	,	,
cord-253024-b393ea2u	333	18	these	these	DT
cord-253024-b393ea2u	333	19	data	datum	NNS
cord-253024-b393ea2u	333	20	have	have	VBP
cord-253024-b393ea2u	333	21	suggested	suggest	VBN
cord-253024-b393ea2u	333	22	that	that	IN
cord-253024-b393ea2u	333	23	the	the	DT
cord-253024-b393ea2u	333	24	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	333	25	ORFs	ORFs	NNP
cord-253024-b393ea2u	333	26	may	may	MD
cord-253024-b393ea2u	333	27	be	be	VB
cord-253024-b393ea2u	333	28	very	very	RB
cord-253024-b393ea2u	333	29	amenable	amenable	JJ
cord-253024-b393ea2u	333	30	to	to	IN
cord-253024-b393ea2u	333	31	frequent	frequent	JJ
cord-253024-b393ea2u	333	32	deletions	deletion	NNS
cord-253024-b393ea2u	333	33	and	and	CC
cord-253024-b393ea2u	333	34	insertions	insertion	NNS
cord-253024-b393ea2u	333	35	.	.	.
cord-253024-b393ea2u	334	1	Insertions	insertion	NNS
cord-253024-b393ea2u	334	2	in	in	IN
cord-253024-b393ea2u	334	3	the	the	DT
cord-253024-b393ea2u	334	4	MHV	MHV	NNP
cord-253024-b393ea2u	334	5	S	S	NNP
cord-253024-b393ea2u	334	6	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	334	7	gene	gene	NN
cord-253024-b393ea2u	334	8	have	have	VBP
cord-253024-b393ea2u	334	9	also	also	RB
cord-253024-b393ea2u	334	10	been	be	VBN
cord-253024-b393ea2u	334	11	reported	report	VBN
cord-253024-b393ea2u	334	12	previously	previously	RB
cord-253024-b393ea2u	335	1	(	(	-LRB-
cord-253024-b393ea2u	335	2	Taguchi	Taguchi	NNP
cord-253024-b393ea2u	335	3	et	et	NNP
cord-253024-b393ea2u	335	4	al	al	NNP
cord-253024-b393ea2u	335	5	.	.	NNP
cord-253024-b393ea2u	335	6	,	,	,
cord-253024-b393ea2u	335	7	1987	1987	CD
cord-253024-b393ea2u	335	8	)	)	-RRB-
cord-253024-b393ea2u	335	9	.	.	.
cord-253024-b393ea2u	336	1	Analysis	analysis	NN
cord-253024-b393ea2u	336	2	of	of	IN
cord-253024-b393ea2u	336	3	20	20	CD
cord-253024-b393ea2u	336	4	RNA	RNA	NNP
cord-253024-b393ea2u	336	5	recombinant	recombinant	JJ
cord-253024-b393ea2u	336	6	viruses	virus	NNS
cord-253024-b393ea2u	336	7	derived	derive	VBN
cord-253024-b393ea2u	336	8	from	from	IN
cord-253024-b393ea2u	336	9	different	different	JJ
cord-253024-b393ea2u	336	10	crosses	crosse	NNS
cord-253024-b393ea2u	336	11	between	between	IN
cord-253024-b393ea2u	336	12	the	the	DT
cord-253024-b393ea2u	336	13	group	group	NN
cord-253024-b393ea2u	336	14	F	F	NNP
cord-253024-b393ea2u	336	15	mutants	mutant	NNS
cord-253024-b393ea2u	336	16	suggested	suggest	VBD
cord-253024-b393ea2u	336	17	that	that	IN
cord-253024-b393ea2u	336	18	large	large	JJ
cord-253024-b393ea2u	336	19	deletions	deletion	NNS
cord-253024-b393ea2u	336	20	or	or	CC
cord-253024-b393ea2u	336	21	insertions	insertion	NNS
cord-253024-b393ea2u	336	22	were	be	VBD
cord-253024-b393ea2u	336	23	rare	rare	JJ
cord-253024-b393ea2u	336	24	and	and	CC
cord-253024-b393ea2u	336	25	occurred	occur	VBD
cord-253024-b393ea2u	336	26	at	at	IN
cord-253024-b393ea2u	336	27	<	<	XX
cord-253024-b393ea2u	336	28	&	&	CC
cord-253024-b393ea2u	336	29	the	the	DT
cord-253024-b393ea2u	336	30	rate	rate	NN
cord-253024-b393ea2u	336	31	of	of	IN
cord-253024-b393ea2u	336	32	true	true	JJ
cord-253024-b393ea2u	336	33	homologous	homologous	JJ
cord-253024-b393ea2u	336	34	recombination	recombination	NN
cord-253024-b393ea2u	336	35	.	.	.
cord-253024-b393ea2u	337	1	While	while	IN
cord-253024-b393ea2u	337	2	these	these	DT
cord-253024-b393ea2u	337	3	data	datum	NNS
cord-253024-b393ea2u	337	4	could	could	MD
cord-253024-b393ea2u	337	5	not	not	RB
cord-253024-b393ea2u	337	6	conclusively	conclusively	RB
cord-253024-b393ea2u	337	7	rule	rule	VB
cord-253024-b393ea2u	337	8	out	out	RP
cord-253024-b393ea2u	337	9	the	the	DT
cord-253024-b393ea2u	337	10	presence	presence	NN
cord-253024-b393ea2u	337	11	of	of	IN
cord-253024-b393ea2u	337	12	very	very	RB
cord-253024-b393ea2u	337	13	small	small	JJ
cord-253024-b393ea2u	337	14	deletions	deletion	NNS
cord-253024-b393ea2u	337	15	,	,	,
cord-253024-b393ea2u	337	16	this	this	DT
cord-253024-b393ea2u	337	17	seems	seem	VBZ
cord-253024-b393ea2u	337	18	unlikely	unlikely	JJ
cord-253024-b393ea2u	337	19	since	since	IN
cord-253024-b393ea2u	337	20	the	the	DT
cord-253024-b393ea2u	337	21	predicted	predict	VBN
cord-253024-b393ea2u	337	22	distances	distance	NNS
cord-253024-b393ea2u	337	23	between	between	IN
cord-253024-b393ea2u	337	24	the	the	DT
cord-253024-b393ea2u	337	25	ts	ts	NNP
cord-253024-b393ea2u	337	26	alleles	allele	NNS
cord-253024-b393ea2u	337	27	used	use	VBN
cord-253024-b393ea2u	337	28	in	in	IN
cord-253024-b393ea2u	337	29	these	these	DT
cord-253024-b393ea2u	337	30	crosses	crosse	NNS
cord-253024-b393ea2u	337	31	should	should	MD
cord-253024-b393ea2u	337	32	have	have	VB
cord-253024-b393ea2u	337	33	resulted	result	VBN
cord-253024-b393ea2u	337	34	in	in	IN
cord-253024-b393ea2u	337	35	deletions	deletion	NNS
cord-253024-b393ea2u	337	36	ranging	range	VBG
cord-253024-b393ea2u	337	37	from	from	IN
cord-253024-b393ea2u	337	38	-100	-100	NNP
cord-253024-b393ea2u	337	39	bp	bp	NNP
cord-253024-b393ea2u	337	40	to	to	IN
cord-253024-b393ea2u	337	41	>	>	XX
cord-253024-b393ea2u	337	42	1000	1000	CD
cord-253024-b393ea2u	337	43	bp	bp	NN
cord-253024-b393ea2u	337	44	in	in	IN
cord-253024-b393ea2u	337	45	length	length	NN
cord-253024-b393ea2u	337	46	.	.	.
cord-253024-b393ea2u	338	1	It	-PRON-	PRP
cord-253024-b393ea2u	338	2	is	be	VBZ
cord-253024-b393ea2u	338	3	also	also	RB
cord-253024-b393ea2u	338	4	unlikely	unlikely	JJ
cord-253024-b393ea2u	338	5	that	that	IN
cord-253024-b393ea2u	338	6	the	the	DT
cord-253024-b393ea2u	338	7	ts+	ts+	NNP
cord-253024-b393ea2u	338	8	virus	virus	NN
cord-253024-b393ea2u	338	9	isolated	isolate	VBN
cord-253024-b393ea2u	338	10	from	from	IN
cord-253024-b393ea2u	338	11	these	these	DT
cord-253024-b393ea2u	338	12	crosses	crosse	NNS
cord-253024-b393ea2u	338	13	were	be	VBD
cord-253024-b393ea2u	338	14	revertants	revertant	NNS
cord-253024-b393ea2u	338	15	containing	contain	VBG
cord-253024-b393ea2u	338	16	small	small	JJ
cord-253024-b393ea2u	338	17	deletions	deletion	NNS
cord-253024-b393ea2u	338	18	(	(	-LRB-
cord-253024-b393ea2u	338	19	~50	~50	NFP
cord-253024-b393ea2u	338	20	bp	bp	NNP
cord-253024-b393ea2u	338	21	)	)	-RRB-
cord-253024-b393ea2u	338	22	in	in	IN
cord-253024-b393ea2u	338	23	a	a	DT
cord-253024-b393ea2u	338	24	singlets	singlet	NNS
cord-253024-b393ea2u	338	25	parent	parent	NN
cord-253024-b393ea2u	338	26	,	,	,
cord-253024-b393ea2u	338	27	since	since	IN
cord-253024-b393ea2u	338	28	the	the	DT
cord-253024-b393ea2u	338	29	ratio	ratio	NN
cord-253024-b393ea2u	338	30	of	of	IN
cord-253024-b393ea2u	338	31	recombinants	recombinant	NNS
cord-253024-b393ea2u	338	32	:	:	:
cord-253024-b393ea2u	338	33	revertants	revertants	NNP
cord-253024-b393ea2u	338	34	was	be	VBD
cord-253024-b393ea2u	338	35	at	at	IN
cord-253024-b393ea2u	338	36	least	least	JJS
cord-253024-b393ea2u	338	37	9	9	CD
cord-253024-b393ea2u	338	38	:	:	SYM
cord-253024-b393ea2u	338	39	1	1	CD
cord-253024-b393ea2u	338	40	or	or	CC
cord-253024-b393ea2u	338	41	greater	great	JJR
cord-253024-b393ea2u	338	42	in	in	IN
cord-253024-b393ea2u	338	43	each	each	DT
cord-253024-b393ea2u	338	44	of	of	IN
cord-253024-b393ea2u	338	45	the	the	DT
cord-253024-b393ea2u	338	46	crosses	crosse	NNS
cord-253024-b393ea2u	338	47	and	and	CC
cord-253024-b393ea2u	338	48	none	none	NN
cord-253024-b393ea2u	338	49	of	of	IN
cord-253024-b393ea2u	338	50	the	the	DT
cord-253024-b393ea2u	338	51	revertants	revertant	NNS
cord-253024-b393ea2u	338	52	analyzed	analyze	VBN
cord-253024-b393ea2u	338	53	from	from	IN
cord-253024-b393ea2u	338	54	each	each	DT
cord-253024-b393ea2u	338	55	parental	parental	JJ
cord-253024-b393ea2u	338	56	ts	ts	NN
cord-253024-b393ea2u	338	57	mutant	mutant	NN
cord-253024-b393ea2u	338	58	had	have	VBD
cord-253024-b393ea2u	338	59	evidence	evidence	NN
cord-253024-b393ea2u	338	60	of	of	IN
cord-253024-b393ea2u	338	61	deletions	deletion	NNS
cord-253024-b393ea2u	338	62	.	.	.
cord-253024-b393ea2u	339	1	Since	since	IN
cord-253024-b393ea2u	339	2	revertants	revertant	NNS
cord-253024-b393ea2u	339	3	of	of	IN
cord-253024-b393ea2u	339	4	SB	SB	NNP
cord-253024-b393ea2u	339	5	ts	ts	NN
cord-253024-b393ea2u	339	6	mutants	mutant	NNS
cord-253024-b393ea2u	339	7	usually	usually	RB
cord-253024-b393ea2u	339	8	contain	contain	VBP
cord-253024-b393ea2u	339	9	single	single	JJ
cord-253024-b393ea2u	339	10	nucleotide	nucleotide	JJ
cord-253024-b393ea2u	339	11	reversions	reversion	NNS
cord-253024-b393ea2u	339	12	at	at	IN
cord-253024-b393ea2u	339	13	the	the	DT
cord-253024-b393ea2u	339	14	site	site	NN
cord-253024-b393ea2u	339	15	of	of	IN
cord-253024-b393ea2u	339	16	mutation	mutation	NN
cord-253024-b393ea2u	340	1	(	(	-LRB-
cord-253024-b393ea2u	340	2	Hahn	Hahn	NNP
cord-253024-b393ea2u	340	3	et	et	NNP
cord-253024-b393ea2u	340	4	al	al	NNP
cord-253024-b393ea2u	340	5	.	.	NNP
cord-253024-b393ea2u	340	6	,	,	,
cord-253024-b393ea2u	340	7	1989a	1989a	CD
cord-253024-b393ea2u	340	8	,	,	,
cord-253024-b393ea2u	340	9	b	b	LS
cord-253024-b393ea2u	340	10	)	)	-RRB-
cord-253024-b393ea2u	340	11	,	,	,
cord-253024-b393ea2u	340	12	the	the	DT
cord-253024-b393ea2u	340	13	most	most	RBS
cord-253024-b393ea2u	340	14	likely	likely	JJ
cord-253024-b393ea2u	340	15	interpretation	interpretation	NN
cord-253024-b393ea2u	340	16	from	from	IN
cord-253024-b393ea2u	340	17	these	these	DT
cord-253024-b393ea2u	340	18	data	datum	NNS
cord-253024-b393ea2u	340	19	is	be	VBZ
cord-253024-b393ea2u	340	20	that	that	IN
cord-253024-b393ea2u	340	21	the	the	DT
cord-253024-b393ea2u	340	22	increase	increase	NN
cord-253024-b393ea2u	340	23	in	in	IN
cord-253024-b393ea2u	340	24	the	the	DT
cord-253024-b393ea2u	340	25	rate	rate	NN
cord-253024-b393ea2u	340	26	of	of	IN
cord-253024-b393ea2u	340	27	recombination	recombination	NN
cord-253024-b393ea2u	340	28	in	in	IN
cord-253024-b393ea2u	340	29	the	the	DT
cord-253024-b393ea2u	340	30	S	S	NNP
cord-253024-b393ea2u	340	31	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	340	32	gene	gene	NN
cord-253024-b393ea2u	340	33	probably	probably	RB
cord-253024-b393ea2u	340	34	reflects	reflect	VBZ
cord-253024-b393ea2u	340	35	an	an	DT
cord-253024-b393ea2u	340	36	increase	increase	NN
cord-253024-b393ea2u	340	37	in	in	IN
cord-253024-b393ea2u	340	38	the	the	DT
cord-253024-b393ea2u	340	39	rate	rate	NN
cord-253024-b393ea2u	340	40	of	of	IN
cord-253024-b393ea2u	340	41	true	true	JJ
cord-253024-b393ea2u	340	42	homologous	homologous	JJ
cord-253024-b393ea2u	340	43	RNA	RNA	NNP
cord-253024-b393ea2u	340	44	recombination	recombination	NN
cord-253024-b393ea2u	340	45	.	.	.
cord-253024-b393ea2u	341	1	If	if	IN
cord-253024-b393ea2u	341	2	the	the	DT
cord-253024-b393ea2u	341	3	mechanism	mechanism	NN
cord-253024-b393ea2u	341	4	for	for	IN
cord-253024-b393ea2u	341	5	homologous	homologous	JJ
cord-253024-b393ea2u	341	6	recombination	recombination	NN
cord-253024-b393ea2u	341	7	and	and	CC
cord-253024-b393ea2u	341	8	recombinations	recombination	NNS
cord-253024-b393ea2u	341	9	resulting	result	VBG
cord-253024-b393ea2u	341	10	in	in	IN
cord-253024-b393ea2u	341	11	deletions	deletion	NNS
cord-253024-b393ea2u	341	12	are	be	VBP
cord-253024-b393ea2u	341	13	similar	similar	JJ
cord-253024-b393ea2u	341	14	as	as	IN
cord-253024-b393ea2u	341	15	suggested	suggest	VBN
cord-253024-b393ea2u	341	16	by	by	IN
cord-253024-b393ea2u	341	17	Banner	Banner	NNP
cord-253024-b393ea2u	341	18	et	et	FW
cord-253024-b393ea2u	341	19	al	al	NNP
cord-253024-b393ea2u	341	20	.	.	NNP
cord-253024-b393ea2u	341	21	,	,	,
cord-253024-b393ea2u	341	22	1990	1990	CD
cord-253024-b393ea2u	341	23	,	,	,
cord-253024-b393ea2u	341	24	these	these	DT
cord-253024-b393ea2u	341	25	data	datum	NNS
cord-253024-b393ea2u	341	26	suggest	suggest	VBP
cord-253024-b393ea2u	341	27	that	that	IN
cord-253024-b393ea2u	341	28	the	the	DT
cord-253024-b393ea2u	341	29	frequency	frequency	NN
cord-253024-b393ea2u	341	30	of	of	IN
cord-253024-b393ea2u	341	31	these	these	DT
cord-253024-b393ea2u	341	32	two	two	CD
cord-253024-b393ea2u	341	33	recombination	recombination	NN
cord-253024-b393ea2u	341	34	events	event	NNS
cord-253024-b393ea2u	341	35	is	be	VBZ
cord-253024-b393ea2u	341	36	very	very	RB
cord-253024-b393ea2u	341	37	different	different	JJ
cord-253024-b393ea2u	341	38	.	.	.
cord-253024-b393ea2u	342	1	Alternatively	alternatively	RB
cord-253024-b393ea2u	342	2	,	,	,
cord-253024-b393ea2u	342	3	the	the	DT
cord-253024-b393ea2u	342	4	regions	region	NNS
cord-253024-b393ea2u	342	5	flanked	flank	VBN
cord-253024-b393ea2u	342	6	by	by	IN
cord-253024-b393ea2u	342	7	the	the	DT
cord-253024-b393ea2u	342	8	ts	ts	NNP
cord-253024-b393ea2u	342	9	mutations	mutation	NNS
cord-253024-b393ea2u	342	10	used	use	VBN
cord-253024-b393ea2u	342	11	in	in	IN
cord-253024-b393ea2u	342	12	these	these	DT
cord-253024-b393ea2u	342	13	crosses	crosse	NNS
cord-253024-b393ea2u	342	14	may	may	MD
cord-253024-b393ea2u	342	15	be	be	VB
cord-253024-b393ea2u	342	16	critical	critical	JJ
cord-253024-b393ea2u	342	17	for	for	IN
cord-253024-b393ea2u	342	18	S	s	NN
cord-253024-b393ea2u	342	19	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	342	20	gene	gene	NN
cord-253024-b393ea2u	342	21	function	function	NN
cord-253024-b393ea2u	342	22	or	or	CC
cord-253024-b393ea2u	342	23	plaque	plaque	NN
cord-253024-b393ea2u	342	24	formation	formation	NN
cord-253024-b393ea2u	342	25	and	and	CC
cord-253024-b393ea2u	342	26	could	could	MD
cord-253024-b393ea2u	342	27	not	not	RB
cord-253024-b393ea2u	342	28	be	be	VB
cord-253024-b393ea2u	342	29	deleted	delete	VBN
cord-253024-b393ea2u	342	30	.	.	.
cord-253024-b393ea2u	343	1	Two	two	CD
cord-253024-b393ea2u	343	2	possible	possible	JJ
cord-253024-b393ea2u	343	3	mechanisms	mechanism	NNS
cord-253024-b393ea2u	343	4	could	could	MD
cord-253024-b393ea2u	343	5	explain	explain	VB
cord-253024-b393ea2u	343	6	variable	variable	JJ
cord-253024-b393ea2u	343	7	rates	rate	NNS
cord-253024-b393ea2u	343	8	of	of	IN
cord-253024-b393ea2u	343	9	recombination	recombination	NN
cord-253024-b393ea2u	343	10	in	in	IN
cord-253024-b393ea2u	343	11	the	the	DT
cord-253024-b393ea2u	343	12	MHV	MHV	NNP
cord-253024-b393ea2u	343	13	genome	genome	NN
cord-253024-b393ea2u	343	14	.	.	.
cord-253024-b393ea2u	344	1	The	the	DT
cord-253024-b393ea2u	344	2	first	first	JJ
cord-253024-b393ea2u	344	3	possibility	possibility	NN
cord-253024-b393ea2u	344	4	is	be	VBZ
cord-253024-b393ea2u	344	5	a	a	DT
cord-253024-b393ea2u	344	6	preferred	preferred	JJ
cord-253024-b393ea2u	344	7	recombination	recombination	NN
cord-253024-b393ea2u	344	8	site	site	NN
cord-253024-b393ea2u	344	9	in	in	IN
cord-253024-b393ea2u	344	10	the	the	DT
cord-253024-b393ea2u	344	11	MHV	MHV	NNP
cord-253024-b393ea2u	344	12	S	S	NNP
cord-253024-b393ea2u	344	13	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	344	14	gene	gene	NN
cord-253024-b393ea2u	344	15	.	.	.
cord-253024-b393ea2u	345	1	A	a	DT
cord-253024-b393ea2u	345	2	clustering	clustering	NN
cord-253024-b393ea2u	345	3	of	of	IN
cord-253024-b393ea2u	345	4	RNA	RNA	NNP
cord-253024-b393ea2u	345	5	recombination	recombination	NN
cord-253024-b393ea2u	345	6	sites	site	NNS
cord-253024-b393ea2u	345	7	adjacent	adjacent	JJ
cord-253024-b393ea2u	345	8	to	to	IN
cord-253024-b393ea2u	345	9	the	the	DT
cord-253024-b393ea2u	345	10	hypervariable	hypervariable	JJ
cord-253024-b393ea2u	345	11	region	region	NN
cord-253024-b393ea2u	345	12	in	in	IN
cord-253024-b393ea2u	345	13	the	the	DT
cord-253024-b393ea2u	345	14	S	S	NNP
cord-253024-b393ea2u	345	15	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	345	16	region	region	NN
cord-253024-b393ea2u	345	17	suggest	suggest	VBP
cord-253024-b393ea2u	345	18	the	the	DT
cord-253024-b393ea2u	345	19	presence	presence	NN
cord-253024-b393ea2u	345	20	of	of	IN
cord-253024-b393ea2u	345	21	a	a	DT
cord-253024-b393ea2u	345	22	preferred	preferred	JJ
cord-253024-b393ea2u	345	23	site	site	NN
cord-253024-b393ea2u	345	24	of	of	IN
cord-253024-b393ea2u	345	25	recombination	recombination	NN
cord-253024-b393ea2u	345	26	(	(	-LRB-
cord-253024-b393ea2u	345	27	Banner	Banner	NNP
cord-253024-b393ea2u	345	28	eta/.	eta/.	CD
cord-253024-b393ea2u	345	29	,	,	,
cord-253024-b393ea2u	345	30	1990	1990	CD
cord-253024-b393ea2u	345	31	)	)	-RRB-
cord-253024-b393ea2u	345	32	.	.	.
cord-253024-b393ea2u	346	1	However	however	RB
cord-253024-b393ea2u	346	2	,	,	,
cord-253024-b393ea2u	346	3	in	in	IN
cord-253024-b393ea2u	346	4	the	the	DT
cord-253024-b393ea2u	346	5	absence	absence	NN
cord-253024-b393ea2u	346	6	of	of	IN
cord-253024-b393ea2u	346	7	selection	selection	NN
cord-253024-b393ea2u	346	8	,	,	,
cord-253024-b393ea2u	346	9	crossover	crossover	NN
cord-253024-b393ea2u	346	10	sites	site	NNS
cord-253024-b393ea2u	346	11	spanning	span	VBG
cord-253024-b393ea2u	346	12	the	the	DT
cord-253024-b393ea2u	346	13	hypervariable	hypervariable	JJ
cord-253024-b393ea2u	346	14	region	region	NN
cord-253024-b393ea2u	346	15	of	of	IN
cord-253024-b393ea2u	346	16	the	the	DT
cord-253024-b393ea2u	346	17	MHV	MHV	NNP
cord-253024-b393ea2u	346	18	S	S	NNP
cord-253024-b393ea2u	346	19	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	346	20	gene	gene	NN
cord-253024-b393ea2u	346	21	were	be	VBD
cord-253024-b393ea2u	346	22	random	random	JJ
cord-253024-b393ea2u	346	23	,	,	,
cord-253024-b393ea2u	346	24	suggesting	suggest	VBG
cord-253024-b393ea2u	346	25	that	that	IN
cord-253024-b393ea2u	346	26	the	the	DT
cord-253024-b393ea2u	346	27	preferred	preferred	JJ
cord-253024-b393ea2u	346	28	site	site	NN
cord-253024-b393ea2u	346	29	of	of	IN
cord-253024-b393ea2u	346	30	recombination	recombination	NN
cord-253024-b393ea2u	346	31	reflects	reflect	VBZ
cord-253024-b393ea2u	346	32	in	in	IN
cord-253024-b393ea2u	346	33	vitro	vitro	FW
cord-253024-b393ea2u	346	34	selection	selection	NN
cord-253024-b393ea2u	346	35	for	for	IN
cord-253024-b393ea2u	346	36	certain	certain	JJ
cord-253024-b393ea2u	346	37	types	type	NNS
cord-253024-b393ea2u	346	38	of	of	IN
cord-253024-b393ea2u	346	39	recombinants	recombinant	NNS
cord-253024-b393ea2u	346	40	.	.	.
cord-253024-b393ea2u	347	1	Although	although	IN
cord-253024-b393ea2u	347	2	the	the	DT
cord-253024-b393ea2u	347	3	majority	majority	NN
cord-253024-b393ea2u	347	4	of	of	IN
cord-253024-b393ea2u	347	5	data	datum	NNS
cord-253024-b393ea2u	347	6	support	support	VBP
cord-253024-b393ea2u	347	7	non	non	JJ
cord-253024-b393ea2u	347	8	-	-	JJ
cord-253024-b393ea2u	347	9	site	site	JJ
cord-253024-b393ea2u	347	10	-	-	HYPH
cord-253024-b393ea2u	347	11	specific	specific	JJ
cord-253024-b393ea2u	347	12	homologous	homologous	JJ
cord-253024-b393ea2u	347	13	recombination	recombination	NN
cord-253024-b393ea2u	347	14	throughout	throughout	IN
cord-253024-b393ea2u	347	15	the	the	DT
cord-253024-b393ea2u	347	16	entire	entire	JJ
cord-253024-b393ea2u	347	17	poliovirus	poliovirus	NN
cord-253024-b393ea2u	347	18	genome	genome	NN
cord-253024-b393ea2u	347	19	(	(	-LRB-
cord-253024-b393ea2u	347	20	Kirkegaard	Kirkegaard	NNP
cord-253024-b393ea2u	347	21	and	and	CC
cord-253024-b393ea2u	347	22	Baltimore	Baltimore	NNP
cord-253024-b393ea2u	347	23	,	,	,
cord-253024-b393ea2u	347	24	1986	1986	CD
cord-253024-b393ea2u	347	25	;	;	:
cord-253024-b393ea2u	347	26	Sarnow	Sarnow	NNP
cord-253024-b393ea2u	347	27	et	et	NNP
cord-253024-b393ea2u	347	28	al	al	NNP
cord-253024-b393ea2u	347	29	,	,	,
cord-253024-b393ea2u	347	30	,	,	,
cord-253024-b393ea2u	347	31	1990	1990	CD
cord-253024-b393ea2u	347	32	)	)	-RRB-
cord-253024-b393ea2u	348	1	Brian	Brian	NNP
cord-253024-b393ea2u	348	2	,	,	,
cord-253024-b393ea2u	348	3	1989	1989	CD
cord-253024-b393ea2u	348	4	,	,	,
cord-253024-b393ea2u	348	5	and	and	CC
cord-253024-b393ea2u	348	6	Sawickr	Sawickr	NNP
cord-253024-b393ea2u	348	7	and	and	CC
cord-253024-b393ea2u	348	8	Sawicki	Sawicki	NNP
cord-253024-b393ea2u	348	9	,	,	,
cord-253024-b393ea2u	348	10	1990	1990	CD
cord-253024-b393ea2u	348	11	,	,	,
cord-253024-b393ea2u	348	12	have	have	VBP
cord-253024-b393ea2u	348	13	clearly	clearly	RB
cord-253024-b393ea2u	348	14	demonstrated	demonstrate	VBN
cord-253024-b393ea2u	348	15	the	the	DT
cord-253024-b393ea2u	348	16	presence	presence	NN
cord-253024-b393ea2u	348	17	of	of	IN
cord-253024-b393ea2u	348	18	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	348	19	minus	minus	CC
cord-253024-b393ea2u	348	20	strands	strand	NNS
cord-253024-b393ea2u	349	1	In	in	IN
cord-253024-b393ea2u	349	2	MHV	MHV	NNP
cord-253024-b393ea2u	349	3	and	and	CC
cord-253024-b393ea2u	349	4	TGEV	TGEV	NNP
cord-253024-b393ea2u	349	5	infected	infected	JJ
cord-253024-b393ea2u	349	6	cells	cell	NNS
cord-253024-b393ea2u	349	7	.	.	.
cord-253024-b393ea2u	350	1	If	if	IN
cord-253024-b393ea2u	350	2	the	the	DT
cord-253024-b393ea2u	350	3	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	350	4	minus	minus	FW
cord-253024-b393ea2u	350	5	strands	strand	NNS
cord-253024-b393ea2u	350	6	partrcipate	partrcipate	VBP
cord-253024-b393ea2u	350	7	In	in	IN
cord-253024-b393ea2u	350	8	template	template	NN
cord-253024-b393ea2u	350	9	switching	switch	VBG
cord-253024-b393ea2u	350	10	with	with	IN
cord-253024-b393ea2u	350	11	full	full	JJ
cord-253024-b393ea2u	350	12	-	-	HYPH
cord-253024-b393ea2u	350	13	length	length	NN
cord-253024-b393ea2u	350	14	genomrc	genomrc	NN
cord-253024-b393ea2u	350	15	RNAs	rna	NNS
cord-253024-b393ea2u	350	16	,	,	,
cord-253024-b393ea2u	350	17	recombrnation	recombrnation	NN
cord-253024-b393ea2u	350	18	rates	rate	NNS
cord-253024-b393ea2u	350	19	should	should	MD
cord-253024-b393ea2u	350	20	increase	increase	VB
cord-253024-b393ea2u	350	21	from	from	IN
cord-253024-b393ea2u	350	22	the	the	DT
cord-253024-b393ea2u	350	23	5	5	CD
cord-253024-b393ea2u	350	24	'	'	''
cord-253024-b393ea2u	350	25	to	to	IN
cord-253024-b393ea2u	350	26	the	the	DT
cord-253024-b393ea2u	350	27	3	3	CD
cord-253024-b393ea2u	350	28	'	'	NN
cord-253024-b393ea2u	350	29	end	end	NN
cord-253024-b393ea2u	350	30	of	of	IN
cord-253024-b393ea2u	350	31	the	the	DT
cord-253024-b393ea2u	350	32	genome	genome	NN
cord-253024-b393ea2u	350	33	.	.	.
cord-253024-b393ea2u	351	1	Panel	Panel	NNP
cord-253024-b393ea2u	351	2	A	A	NNP
cord-253024-b393ea2u	351	3	demonstrates	demonstrate	VBZ
cord-253024-b393ea2u	351	4	how	how	WRB
cord-253024-b393ea2u	351	5	template	template	NN
cord-253024-b393ea2u	351	6	switching	switching	NN
cord-253024-b393ea2u	351	7	could	could	MD
cord-253024-b393ea2u	351	8	occur	occur	VB
cord-253024-b393ea2u	351	9	during	during	IN
cord-253024-b393ea2u	351	10	negative	negative	JJ
cord-253024-b393ea2u	351	11	-	-	HYPH
cord-253024-b393ea2u	351	12	strand	strand	NN
cord-253024-b393ea2u	351	13	synthesis	synthesis	NN
cord-253024-b393ea2u	351	14	between	between	IN
cord-253024-b393ea2u	351	15	a	a	DT
cord-253024-b393ea2u	351	16	full	full	JJ
cord-253024-b393ea2u	351	17	-	-	HYPH
cord-253024-b393ea2u	351	18	length	length	NN
cord-253024-b393ea2u	351	19	and	and	CC
cord-253024-b393ea2u	351	20	subgenomic	subgenomic	JJ
cord-253024-b393ea2u	351	21	-	-	HYPH
cord-253024-b393ea2u	351	22	length	length	NN
cord-253024-b393ea2u	351	23	plus	plus	CC
cord-253024-b393ea2u	351	24	strand	strand	NN
cord-253024-b393ea2u	351	25	RNA	RNA	NNP
cord-253024-b393ea2u	351	26	and	and	CC
cord-253024-b393ea2u	351	27	result	result	VB
cord-253024-b393ea2u	352	1	In	in	IN
cord-253024-b393ea2u	352	2	a	a	DT
cord-253024-b393ea2u	352	3	full	full	JJ
cord-253024-b393ea2u	352	4	-	-	HYPH
cord-253024-b393ea2u	352	5	length	length	NN
cord-253024-b393ea2u	352	6	negative	negative	JJ
cord-253024-b393ea2u	352	7	-	-	HYPH
cord-253024-b393ea2u	352	8	stranded	strand	VBN
cord-253024-b393ea2u	352	9	RNA	RNA	NNP
cord-253024-b393ea2u	352	10	recombinant	recombinant	JJ
cord-253024-b393ea2u	352	11	molecule	molecule	NN
cord-253024-b393ea2u	352	12	.	.	.
cord-253024-b393ea2u	353	1	Panel	Panel	NNP
cord-253024-b393ea2u	353	2	B	B	NNP
cord-253024-b393ea2u	353	3	demonstrates	demonstrate	VBZ
cord-253024-b393ea2u	353	4	how	how	WRB
cord-253024-b393ea2u	353	5	template	template	NN
cord-253024-b393ea2u	353	6	switching	switch	VBG
cord-253024-b393ea2u	353	7	between	between	IN
cord-253024-b393ea2u	353	8	full	full	JJ
cord-253024-b393ea2u	353	9	-	-	HYPH
cord-253024-b393ea2u	353	10	length	length	NN
cord-253024-b393ea2u	353	11	and	and	CC
cord-253024-b393ea2u	353	12	subgenomic	subgenomic	JJ
cord-253024-b393ea2u	353	13	negative	negative	JJ
cord-253024-b393ea2u	353	14	strands	strand	NNS
cord-253024-b393ea2u	353	15	during	during	IN
cord-253024-b393ea2u	353	16	positrve	positrve	JJ
cord-253024-b393ea2u	353	17	-	-	HYPH
cord-253024-b393ea2u	353	18	strand	strand	NN
cord-253024-b393ea2u	353	19	synthesis	synthesis	NN
cord-253024-b393ea2u	353	20	could	could	MD
cord-253024-b393ea2u	353	21	result	result	VB
cord-253024-b393ea2u	353	22	in	in	IN
cord-253024-b393ea2u	353	23	recombinant	recombinant	JJ
cord-253024-b393ea2u	353	24	genome	genome	NN
cord-253024-b393ea2u	353	25	-	-	HYPH
cord-253024-b393ea2u	353	26	length	length	NN
cord-253024-b393ea2u	353	27	molecules	molecule	NNS
cord-253024-b393ea2u	353	28	,	,	,
cord-253024-b393ea2u	353	29	switching	switch	VBG
cord-253024-b393ea2u	353	30	occur	occur	VBP
cord-253024-b393ea2u	353	31	after	after	IN
cord-253024-b393ea2u	353	32	the	the	DT
cord-253024-b393ea2u	353	33	synthesis	synthesis	NN
cord-253024-b393ea2u	353	34	of	of	IN
cord-253024-b393ea2u	353	35	UU	UU	NNP
cord-253024-b393ea2u	353	36	in	in	IN
cord-253024-b393ea2u	353	37	sites	site	NNS
cord-253024-b393ea2u	353	38	chosen	choose	VBN
cord-253024-b393ea2u	353	39	to	to	TO
cord-253024-b393ea2u	353	40	minimize	minimize	VB
cord-253024-b393ea2u	353	41	the	the	DT
cord-253024-b393ea2u	353	42	adverse	adverse	JJ
cord-253024-b393ea2u	353	43	free	free	JJ
cord-253024-b393ea2u	353	44	energy	energy	NN
cord-253024-b393ea2u	353	45	change	change	NN
cord-253024-b393ea2u	353	46	involved	involve	VBN
cord-253024-b393ea2u	353	47	in	in	IN
cord-253024-b393ea2u	353	48	switching	switch	VBG
cord-253024-b393ea2u	353	49	to	to	IN
cord-253024-b393ea2u	353	50	a	a	DT
cord-253024-b393ea2u	353	51	heterotypic	heterotypic	JJ
cord-253024-b393ea2u	353	52	template	template	NN
cord-253024-b393ea2u	353	53	(	(	-LRB-
cord-253024-b393ea2u	353	54	King	King	NNP
cord-253024-b393ea2u	353	55	,	,	,
cord-253024-b393ea2u	353	56	1988	1988	CD
cord-253024-b393ea2u	353	57	)	)	-RRB-
cord-253024-b393ea2u	353	58	.	.	.
cord-253024-b393ea2u	354	1	In	in	IN
cord-253024-b393ea2u	354	2	addition	addition	NN
cord-253024-b393ea2u	354	3	,	,	,
cord-253024-b393ea2u	354	4	a	a	DT
cord-253024-b393ea2u	354	5	nonrandom	nonrandom	JJ
cord-253024-b393ea2u	354	6	distribution	distribution	NN
cord-253024-b393ea2u	354	7	of	of	IN
cord-253024-b393ea2u	354	8	recombination	recombination	NN
cord-253024-b393ea2u	354	9	sites	site	NNS
cord-253024-b393ea2u	354	10	has	have	VBZ
cord-253024-b393ea2u	354	11	also	also	RB
cord-253024-b393ea2u	354	12	been	be	VBN
cord-253024-b393ea2u	354	13	reported	report	VBN
cord-253024-b393ea2u	354	14	among	among	IN
cord-253024-b393ea2u	354	15	intertypic	intertypic	NNP
cord-253024-b393ea2u	354	16	poliovirus	poliovirus	NN
cord-253024-b393ea2u	354	17	recombinants	recombinant	NNS
cord-253024-b393ea2u	354	18	(	(	-LRB-
cord-253024-b393ea2u	354	19	Tolskaya	Tolskaya	NNP
cord-253024-b393ea2u	354	20	et	et	FW
cord-253024-b393ea2u	354	21	al	al	NNP
cord-253024-b393ea2u	354	22	.	.	NNP
cord-253024-b393ea2u	354	23	,	,	,
cord-253024-b393ea2u	354	24	1987	1987	CD
cord-253024-b393ea2u	354	25	)	)	-RRB-
cord-253024-b393ea2u	354	26	.	.	.
cord-253024-b393ea2u	355	1	If	if	IN
cord-253024-b393ea2u	355	2	MHV	MHV	NNP
cord-253024-b393ea2u	355	3	RNA	RNA	NNP
cord-253024-b393ea2u	355	4	recombination	recombination	NN
cord-253024-b393ea2u	355	5	is	be	VBZ
cord-253024-b393ea2u	355	6	mediated	mediate	VBN
cord-253024-b393ea2u	355	7	by	by	IN
cord-253024-b393ea2u	355	8	freely	freely	RB
cord-253024-b393ea2u	355	9	segregating	segregate	VBG
cord-253024-b393ea2u	355	10	RNA	rna	NN
cord-253024-b393ea2u	355	11	segments	segment	NNS
cord-253024-b393ea2u	355	12	that	that	WDT
cord-253024-b393ea2u	355	13	are	be	VBP
cord-253024-b393ea2u	355	14	generated	generate	VBN
cord-253024-b393ea2u	355	15	by	by	IN
cord-253024-b393ea2u	355	16	transcriptional	transcriptional	NN
cord-253024-b393ea2u	355	17	pausing	pause	VBG
cord-253024-b393ea2u	355	18	during	during	IN
cord-253024-b393ea2u	355	19	RNA	RNA	NNP
cord-253024-b393ea2u	355	20	synthesis	synthesis	NN
cord-253024-b393ea2u	355	21	,	,	,
cord-253024-b393ea2u	355	22	preferred	preferred	JJ
cord-253024-b393ea2u	355	23	sites	site	NNS
cord-253024-b393ea2u	355	24	of	of	IN
cord-253024-b393ea2u	355	25	recombination	recombination	NN
cord-253024-b393ea2u	355	26	may	may	MD
cord-253024-b393ea2u	355	27	also	also	RB
cord-253024-b393ea2u	355	28	exist	exist	VB
cord-253024-b393ea2u	355	29	in	in	IN
cord-253024-b393ea2u	355	30	AU	au	JJ
cord-253024-b393ea2u	355	31	-	-	HYPH
cord-253024-b393ea2u	355	32	rich	rich	JJ
cord-253024-b393ea2u	355	33	regions	region	NNS
cord-253024-b393ea2u	356	1	and/or	and/or	CC
cord-253024-b393ea2u	356	2	in	in	IN
cord-253024-b393ea2u	356	3	regions	region	NNS
cord-253024-b393ea2u	356	4	of	of	IN
cord-253024-b393ea2u	356	5	secondary	secondary	JJ
cord-253024-b393ea2u	356	6	structure	structure	NN
cord-253024-b393ea2u	356	7	in	in	IN
cord-253024-b393ea2u	356	8	the	the	DT
cord-253024-b393ea2u	356	9	MHV	MHV	NNP
cord-253024-b393ea2u	356	10	genome	genome	NN
cord-253024-b393ea2u	357	1	(	(	-LRB-
cord-253024-b393ea2u	357	2	Baric	Baric	NNP
cord-253024-b393ea2u	357	3	et	et	NNP
cord-253024-b393ea2u	357	4	al	al	NNP
cord-253024-b393ea2u	357	5	.	.	NNP
cord-253024-b393ea2u	357	6	,	,	,
cord-253024-b393ea2u	357	7	1987	1987	CD
cord-253024-b393ea2u	357	8	)	)	-RRB-
cord-253024-b393ea2u	357	9	.	.	.
cord-253024-b393ea2u	358	1	However	however	RB
cord-253024-b393ea2u	358	2	,	,	,
cord-253024-b393ea2u	358	3	it	-PRON-	PRP
cord-253024-b393ea2u	358	4	is	be	VBZ
cord-253024-b393ea2u	358	5	difficult	difficult	JJ
cord-253024-b393ea2u	358	6	to	to	TO
cord-253024-b393ea2u	358	7	envision	envision	VB
cord-253024-b393ea2u	358	8	how	how	WRB
cord-253024-b393ea2u	358	9	these	these	DT
cord-253024-b393ea2u	358	10	types	type	NNS
cord-253024-b393ea2u	358	11	of	of	IN
cord-253024-b393ea2u	358	12	preferred	preferred	JJ
cord-253024-b393ea2u	358	13	sites	site	NNS
cord-253024-b393ea2u	358	14	of	of	IN
cord-253024-b393ea2u	358	15	recombination	recombination	NN
cord-253024-b393ea2u	358	16	would	would	MD
cord-253024-b393ea2u	358	17	result	result	VB
cord-253024-b393ea2u	358	18	in	in	IN
cord-253024-b393ea2u	358	19	higher	high	JJR
cord-253024-b393ea2u	358	20	intratypic	intratypic	JJ
cord-253024-b393ea2u	358	21	recombination	recombination	NN
cord-253024-b393ea2u	358	22	frequencies	frequency	NNS
cord-253024-b393ea2u	358	23	between	between	IN
cord-253024-b393ea2u	358	24	MHV	MHV	NNP
cord-253024-b393ea2u	358	25	-	-	HYPH
cord-253024-b393ea2u	358	26	A59	A59	NNP
cord-253024-b393ea2u	358	27	ts	ts	NN
cord-253024-b393ea2u	358	28	mutants	mutant	NNS
cord-253024-b393ea2u	358	29	since	since	IN
cord-253024-b393ea2u	358	30	extensive	extensive	JJ
cord-253024-b393ea2u	358	31	secondary	secondary	JJ
cord-253024-b393ea2u	358	32	structure	structure	NN
cord-253024-b393ea2u	358	33	is	be	VBZ
cord-253024-b393ea2u	358	34	found	find	VBN
cord-253024-b393ea2u	358	35	throughout	throughout	IN
cord-253024-b393ea2u	358	36	the	the	DT
cord-253024-b393ea2u	358	37	MHV	MHV	NNP
cord-253024-b393ea2u	358	38	genome	genome	NN
cord-253024-b393ea2u	358	39	and	and	CC
cord-253024-b393ea2u	358	40	the	the	DT
cord-253024-b393ea2u	358	41	G	g	NN
cord-253024-b393ea2u	359	1	:	:	:
cord-253024-b393ea2u	359	2	C	c	NN
cord-253024-b393ea2u	359	3	:	:	:
cord-253024-b393ea2u	360	1	A	a	DT
cord-253024-b393ea2u	360	2	:	:	:
cord-253024-b393ea2u	361	1	U	U	NNP
cord-253024-b393ea2u	361	2	and	and	CC
cord-253024-b393ea2u	361	3	AA	AA	NNP
cord-253024-b393ea2u	361	4	/	/	SYM
cord-253024-b393ea2u	361	5	UU	UU	NNP
cord-253024-b393ea2u	361	6	dimer	dimer	NN
cord-253024-b393ea2u	361	7	ratios	ratio	NNS
cord-253024-b393ea2u	361	8	are	be	VBP
cord-253024-b393ea2u	361	9	roughly	roughly	RB
cord-253024-b393ea2u	361	10	equivalent	equivalent	JJ
cord-253024-b393ea2u	361	11	in	in	IN
cord-253024-b393ea2u	361	12	the	the	DT
cord-253024-b393ea2u	361	13	polymerase	polymerase	NN
cord-253024-b393ea2u	361	14	and	and	CC
cord-253024-b393ea2u	361	15	S	S	NNP
cord-253024-b393ea2u	361	16	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	361	17	regions	region	NNS
cord-253024-b393ea2u	361	18	(	(	-LRB-
cord-253024-b393ea2u	361	19	Banner	Banner	NNP
cord-253024-b393ea2u	361	20	et	et	FW
cord-253024-b393ea2u	361	21	al	al	NNP
cord-253024-b393ea2u	361	22	.	.	NNP
cord-253024-b393ea2u	361	23	,	,	,
cord-253024-b393ea2u	361	24	199	199	CD
cord-253024-b393ea2u	361	25	1	1	CD
cord-253024-b393ea2u	361	26	;	;	:
cord-253024-b393ea2u	361	27	Baric	Baric	NNP
cord-253024-b393ea2u	361	28	et	et	NNP
cord-253024-b393ea2u	361	29	al	al	NNP
cord-253024-b393ea2u	361	30	.	.	NNP
cord-253024-b393ea2u	361	31	,	,	,
cord-253024-b393ea2u	361	32	1987	1987	CD
cord-253024-b393ea2u	361	33	;	;	.
cord-253024-b393ea2u	362	1	Soe	Soe	NNP
cord-253024-b393ea2u	362	2	et	et	FW
cord-253024-b393ea2u	362	3	al	al	NNP
cord-253024-b393ea2u	362	4	.	.	NNP
cord-253024-b393ea2u	362	5	,	,	,
cord-253024-b393ea2u	362	6	1988	1988	CD
cord-253024-b393ea2u	362	7	;	;	.
cord-253024-b393ea2u	363	1	Fu	Fu	NNP
cord-253024-b393ea2u	363	2	et	et	FW
cord-253024-b393ea2u	363	3	al	al	NNP
cord-253024-b393ea2u	363	4	.	.	.
cord-253024-b393ea2u	364	1	,	,	,
cord-253024-b393ea2u	364	2	unpublished	unpublished	JJ
cord-253024-b393ea2u	364	3	)	)	-RRB-
cord-253024-b393ea2u	364	4	.	.	.
cord-253024-b393ea2u	365	1	A	a	DT
cord-253024-b393ea2u	365	2	more	more	RBR
cord-253024-b393ea2u	365	3	likely	likely	JJ
cord-253024-b393ea2u	365	4	mechanism	mechanism	NN
cord-253024-b393ea2u	365	5	to	to	TO
cord-253024-b393ea2u	365	6	explain	explain	VB
cord-253024-b393ea2u	365	7	the	the	DT
cord-253024-b393ea2u	365	8	increase	increase	NN
cord-253024-b393ea2u	365	9	in	in	IN
cord-253024-b393ea2u	365	10	the	the	DT
cord-253024-b393ea2u	365	11	recombination	recombination	NN
cord-253024-b393ea2u	365	12	rate	rate	NN
cord-253024-b393ea2u	365	13	in	in	IN
cord-253024-b393ea2u	365	14	the	the	DT
cord-253024-b393ea2u	365	15	S	S	NNP
cord-253024-b393ea2u	365	16	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	365	17	gene	gene	NN
cord-253024-b393ea2u	365	18	is	be	VBZ
cord-253024-b393ea2u	365	19	based	base	VBN
cord-253024-b393ea2u	365	20	on	on	IN
cord-253024-b393ea2u	365	21	the	the	DT
cord-253024-b393ea2u	365	22	basic	basic	JJ
cord-253024-b393ea2u	365	23	replication	replication	NN
cord-253024-b393ea2u	365	24	strategy	strategy	NN
cord-253024-b393ea2u	365	25	of	of	IN
cord-253024-b393ea2u	365	26	coronaviruses	coronaviruse	NNS
cord-253024-b393ea2u	365	27	(	(	-LRB-
cord-253024-b393ea2u	365	28	Fig	Fig	NNP
cord-253024-b393ea2u	365	29	.	.	NNP
cord-253024-b393ea2u	365	30	7	7	CD
cord-253024-b393ea2u	365	31	)	)	-RRB-
cord-253024-b393ea2u	365	32	.	.	.
cord-253024-b393ea2u	366	1	Recently	recently	RB
cord-253024-b393ea2u	366	2	,	,	,
cord-253024-b393ea2u	366	3	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	366	4	minus	minus	JJ
cord-253024-b393ea2u	366	5	-	-	HYPH
cord-253024-b393ea2u	366	6	strand	strand	NN
cord-253024-b393ea2u	366	7	and	and	CC
cord-253024-b393ea2u	366	8	replicative	replicative	JJ
cord-253024-b393ea2u	366	9	intermediate	intermediate	JJ
cord-253024-b393ea2u	366	10	RNAs	rna	NNS
cord-253024-b393ea2u	366	11	have	have	VBP
cord-253024-b393ea2u	366	12	been	be	VBN
cord-253024-b393ea2u	366	13	demonstrated	demonstrate	VBN
cord-253024-b393ea2u	366	14	during	during	IN
cord-253024-b393ea2u	366	15	TGEV	TGEV	NNP
cord-253024-b393ea2u	366	16	and	and	CC
cord-253024-b393ea2u	366	17	MHV	MHV	NNP
cord-253024-b393ea2u	366	18	infection	infection	NN
cord-253024-b393ea2u	366	19	(	(	-LRB-
cord-253024-b393ea2u	366	20	Sethna	Sethna	NNP
cord-253024-b393ea2u	366	21	et	et	NNP
cord-253024-b393ea2u	366	22	a/.	a/.	NN
cord-253024-b393ea2u	366	23	,	,	,
cord-253024-b393ea2u	366	24	1989	1989	CD
cord-253024-b393ea2u	366	25	(	(	-LRB-
cord-253024-b393ea2u	366	26	Sethna	Sethna	NNP
cord-253024-b393ea2u	366	27	et	et	NNP
cord-253024-b393ea2u	366	28	a/.	a/.	NNP
cord-253024-b393ea2u	366	29	,	,	,
cord-253024-b393ea2u	366	30	,	,	,
cord-253024-b393ea2u	367	1	1991	1991	CD
cord-253024-b393ea2u	367	2	Sawicki	Sawicki	NNP
cord-253024-b393ea2u	367	3	and	and	CC
cord-253024-b393ea2u	367	4	Sawicki	Sawicki	NNP
cord-253024-b393ea2u	367	5	,	,	,
cord-253024-b393ea2u	367	6	1990	1990	CD
cord-253024-b393ea2u	367	7	)	)	-RRB-
cord-253024-b393ea2u	367	8	.	.	.
cord-253024-b393ea2u	368	1	These	these	DT
cord-253024-b393ea2u	368	2	data	datum	NNS
cord-253024-b393ea2u	368	3	indicate	indicate	VBP
cord-253024-b393ea2u	368	4	that	that	IN
cord-253024-b393ea2u	368	5	the	the	DT
cord-253024-b393ea2u	368	6	amount	amount	NN
cord-253024-b393ea2u	368	7	of	of	IN
cord-253024-b393ea2u	368	8	negative	negative	JJ
cord-253024-b393ea2u	368	9	-	-	HYPH
cord-253024-b393ea2u	368	10	strand	strand	NN
cord-253024-b393ea2u	368	11	template	template	NN
cord-253024-b393ea2u	368	12	RNA	RNA	NNP
cord-253024-b393ea2u	368	13	is	be	VBZ
cord-253024-b393ea2u	368	14	unequal	unequal	JJ
cord-253024-b393ea2u	368	15	and	and	CC
cord-253024-b393ea2u	368	16	increases	increase	NNS
cord-253024-b393ea2u	368	17	from	from	IN
cord-253024-b393ea2u	368	18	the	the	DT
cord-253024-b393ea2u	368	19	5	5	CD
cord-253024-b393ea2u	368	20	'	'	''
cord-253024-b393ea2u	368	21	to	to	IN
cord-253024-b393ea2u	368	22	3	3	CD
cord-253024-b393ea2u	368	23	'	'	''
cord-253024-b393ea2u	368	24	end	end	NN
cord-253024-b393ea2u	368	25	of	of	IN
cord-253024-b393ea2u	368	26	the	the	DT
cord-253024-b393ea2u	368	27	genome	genome	NN
cord-253024-b393ea2u	368	28	.	.	.
cord-253024-b393ea2u	369	1	Because	because	IN
cord-253024-b393ea2u	369	2	of	of	IN
cord-253024-b393ea2u	369	3	the	the	DT
cord-253024-b393ea2u	369	4	availability	availability	NN
cord-253024-b393ea2u	369	5	of	of	IN
cord-253024-b393ea2u	369	6	more	more	RBR
cord-253024-b393ea2u	369	7	negative	negative	JJ
cord-253024-b393ea2u	369	8	strand	strand	NN
cord-253024-b393ea2u	369	9	template	template	NN
cord-253024-b393ea2u	369	10	,	,	,
cord-253024-b393ea2u	369	11	recombination	recombination	NN
cord-253024-b393ea2u	369	12	rates	rate	NNS
cord-253024-b393ea2u	369	13	should	should	MD
cord-253024-b393ea2u	369	14	increase	increase	VB
cord-253024-b393ea2u	369	15	proportionately	proportionately	RB
cord-253024-b393ea2u	369	16	from	from	IN
cord-253024-b393ea2u	369	17	the	the	DT
cord-253024-b393ea2u	369	18	5	5	CD
cord-253024-b393ea2u	369	19	'	'	''
cord-253024-b393ea2u	369	20	to	to	IN
cord-253024-b393ea2u	369	21	3	3	CD
cord-253024-b393ea2u	369	22	'	'	''
cord-253024-b393ea2u	369	23	end	end	NN
cord-253024-b393ea2u	369	24	of	of	IN
cord-253024-b393ea2u	369	25	the	the	DT
cord-253024-b393ea2u	369	26	genome	genome	NN
cord-253024-b393ea2u	369	27	and	and	CC
cord-253024-b393ea2u	369	28	be	be	VB
cord-253024-b393ea2u	369	29	highest	high	JJS
cord-253024-b393ea2u	369	30	in	in	IN
cord-253024-b393ea2u	369	31	the	the	DT
cord-253024-b393ea2u	369	32	N	n	CD
cord-253024-b393ea2u	369	33	gene	gene	NN
cord-253024-b393ea2u	369	34	coding	code	VBG
cord-253024-b393ea2u	369	35	region	region	NN
cord-253024-b393ea2u	369	36	contained	contain	VBN
cord-253024-b393ea2u	369	37	within	within	IN
cord-253024-b393ea2u	369	38	mRNA	mRNA	NNP
cord-253024-b393ea2u	369	39	7	7	CD
cord-253024-b393ea2u	369	40	.	.	.
cord-253024-b393ea2u	370	1	For	for	IN
cord-253024-b393ea2u	370	2	example	example	NN
cord-253024-b393ea2u	370	3	,	,	,
cord-253024-b393ea2u	370	4	recombination	recombination	NN
cord-253024-b393ea2u	370	5	events	event	NNS
cord-253024-b393ea2u	370	6	in	in	IN
cord-253024-b393ea2u	370	7	the	the	DT
cord-253024-b393ea2u	370	8	polymerase	polymerase	NN
cord-253024-b393ea2u	370	9	region	region	NN
cord-253024-b393ea2u	370	10	can	can	MD
cord-253024-b393ea2u	370	11	only	only	RB
cord-253024-b393ea2u	370	12	involve	involve	VB
cord-253024-b393ea2u	370	13	template	template	NN
cord-253024-b393ea2u	370	14	switching	switch	VBG
cord-253024-b393ea2u	370	15	between	between	IN
cord-253024-b393ea2u	370	16	full	full	JJ
cord-253024-b393ea2u	370	17	-	-	HYPH
cord-253024-b393ea2u	370	18	length	length	NN
cord-253024-b393ea2u	370	19	negative	negative	JJ
cord-253024-b393ea2u	370	20	-	-	:
cord-253024-b393ea2u	370	21	or	or	CC
cord-253024-b393ea2u	370	22	positive	positive	JJ
cord-253024-b393ea2u	370	23	-	-	HYPH
cord-253024-b393ea2u	370	24	strand	strand	NN
cord-253024-b393ea2u	370	25	RNA	rna	NN
cord-253024-b393ea2u	370	26	templates	template	NNS
cord-253024-b393ea2u	370	27	.	.	.
cord-253024-b393ea2u	371	1	However	however	RB
cord-253024-b393ea2u	371	2	,	,	,
cord-253024-b393ea2u	371	3	recombination	recombination	NN
cord-253024-b393ea2u	371	4	events	event	NNS
cord-253024-b393ea2u	371	5	in	in	IN
cord-253024-b393ea2u	371	6	the	the	DT
cord-253024-b393ea2u	371	7	S	S	NNP
cord-253024-b393ea2u	371	8	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	371	9	gene	gene	NN
cord-253024-b393ea2u	371	10	or	or	CC
cord-253024-b393ea2u	371	11	other	other	JJ
cord-253024-b393ea2u	371	12	subgenomic	subgenomic	JJ
cord-253024-b393ea2u	371	13	ORFs	ORFs	NNP
cord-253024-b393ea2u	371	14	could	could	MD
cord-253024-b393ea2u	371	15	not	not	RB
cord-253024-b393ea2u	371	16	only	only	RB
cord-253024-b393ea2u	371	17	occur	occur	VB
cord-253024-b393ea2u	371	18	between	between	IN
cord-253024-b393ea2u	371	19	full	full	JJ
cord-253024-b393ea2u	371	20	-	-	HYPH
cord-253024-b393ea2u	371	21	length	length	NN
cord-253024-b393ea2u	371	22	RNA	rna	NN
cord-253024-b393ea2u	371	23	templates	template	NNS
cord-253024-b393ea2u	371	24	but	but	CC
cord-253024-b393ea2u	371	25	also	also	RB
cord-253024-b393ea2u	371	26	involve	involve	VBP
cord-253024-b393ea2u	371	27	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	371	28	mRNA	mRNA	NNP
cord-253024-b393ea2u	371	29	and	and	CC
cord-253024-b393ea2u	371	30	subgenomic	subgenomic	JJ
cord-253024-b393ea2u	371	31	negative	negative	JJ
cord-253024-b393ea2u	371	32	-	-	HYPH
cord-253024-b393ea2u	371	33	strand	strand	NN
cord-253024-b393ea2u	371	34	RNA	RNA	NNP
cord-253024-b393ea2u	371	35	templates	template	NNS
cord-253024-b393ea2u	371	36	as	as	RB
cord-253024-b393ea2u	371	37	well	well	RB
cord-253024-b393ea2u	371	38	.	.	.
cord-253024-b393ea2u	372	1	Several	several	JJ
cord-253024-b393ea2u	372	2	findings	finding	NNS
cord-253024-b393ea2u	372	3	support	support	VBP
cord-253024-b393ea2u	372	4	this	this	DT
cord-253024-b393ea2u	372	5	hypothesis	hypothesis	NN
cord-253024-b393ea2u	372	6	.	.	.
cord-253024-b393ea2u	373	1	First	first	RB
cord-253024-b393ea2u	373	2	,	,	,
cord-253024-b393ea2u	373	3	recombination	recombination	NN
cord-253024-b393ea2u	373	4	frequencies	frequency	NNS
cord-253024-b393ea2u	373	5	between	between	IN
cord-253024-b393ea2u	373	6	the	the	DT
cord-253024-b393ea2u	373	7	polymerase	polymerase	NN
cord-253024-b393ea2u	373	8	gene	gene	NN
cord-253024-b393ea2u	373	9	and	and	CC
cord-253024-b393ea2u	373	10	S	S	NNP
cord-253024-b393ea2u	373	11	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	373	12	genes	gene	NNS
cord-253024-b393ea2u	373	13	of	of	IN
cord-253024-b393ea2u	373	14	MHV	MHV	NNP
cord-253024-b393ea2u	373	15	may	may	MD
cord-253024-b393ea2u	373	16	vary	vary	VB
cord-253024-b393ea2u	373	17	threefold	threefold	RB
cord-253024-b393ea2u	373	18	.	.	.
cord-253024-b393ea2u	374	1	Second	second	RB
cord-253024-b393ea2u	374	2	,	,	,
cord-253024-b393ea2u	374	3	in	in	IN
cord-253024-b393ea2u	374	4	vitro	vitro	FW
cord-253024-b393ea2u	374	5	transcribed	transcribe	VBN
cord-253024-b393ea2u	374	6	subgenomic	subgenomic	JJ
cord-253024-b393ea2u	374	7	-	-	HYPH
cord-253024-b393ea2u	374	8	length	length	NN
cord-253024-b393ea2u	374	9	DI	DI	NNP
cord-253024-b393ea2u	374	10	RNAs	rna	NNS
cord-253024-b393ea2u	374	11	rapidly	rapidly	RB
cord-253024-b393ea2u	374	12	undergo	undergo	VBP
cord-253024-b393ea2u	374	13	RNA	RNA	NNP
cord-253024-b393ea2u	374	14	recombination	recombination	NN
cord-253024-b393ea2u	374	15	with	with	IN
cord-253024-b393ea2u	374	16	fulllength	fulllength	NN
cord-253024-b393ea2u	374	17	(	(	-LRB-
cord-253024-b393ea2u	374	18	or	or	CC
cord-253024-b393ea2u	374	19	subgenomic	subgenomic	JJ
cord-253024-b393ea2u	374	20	)	)	-RRB-
cord-253024-b393ea2u	375	1	RNAs	rna	NNS
cord-253024-b393ea2u	375	2	supporting	support	VBG
cord-253024-b393ea2u	375	3	the	the	DT
cord-253024-b393ea2u	375	4	notion	notion	NN
cord-253024-b393ea2u	375	5	that	that	IN
cord-253024-b393ea2u	375	6	recombination	recombination	NN
cord-253024-b393ea2u	375	7	events	event	NNS
cord-253024-b393ea2u	375	8	can	can	MD
cord-253024-b393ea2u	375	9	occur	occur	VB
cord-253024-b393ea2u	375	10	between	between	IN
cord-253024-b393ea2u	375	11	different	different	JJ
cord-253024-b393ea2u	375	12	-	-	HYPH
cord-253024-b393ea2u	375	13	sized	sized	JJ
cord-253024-b393ea2u	375	14	template	template	NN
cord-253024-b393ea2u	375	15	RNAs	rna	NNS
cord-253024-b393ea2u	375	16	.	.	.
cord-253024-b393ea2u	376	1	Third	third	JJ
cord-253024-b393ea2u	376	2	,	,	,
cord-253024-b393ea2u	376	3	analysis	analysis	NN
cord-253024-b393ea2u	376	4	of	of	IN
cord-253024-b393ea2u	376	5	sepharose	sepharose	NNP
cord-253024-b393ea2u	376	6	2B	2B	NNP
cord-253024-b393ea2u	376	7	-	-	HYPH
cord-253024-b393ea2u	376	8	Cl	Cl	NNP
cord-253024-b393ea2u	376	9	column	column	NN
cord-253024-b393ea2u	376	10	purified	purify	VBN
cord-253024-b393ea2u	376	11	full	full	JJ
cord-253024-b393ea2u	376	12	-	-	HYPH
cord-253024-b393ea2u	376	13	length	length	NN
cord-253024-b393ea2u	376	14	RI	RI	NNP
cord-253024-b393ea2u	376	15	RNA	RNA	NNP
cord-253024-b393ea2u	376	16	has	have	VBZ
cord-253024-b393ea2u	376	17	demonstrated	demonstrate	VBN
cord-253024-b393ea2u	376	18	the	the	DT
cord-253024-b393ea2u	376	19	presence	presence	NN
cord-253024-b393ea2u	376	20	of	of	IN
cord-253024-b393ea2u	376	21	mRNA	mRNA	NNP
cord-253024-b393ea2u	376	22	7	7	CD
cord-253024-b393ea2u	376	23	subgenomic	subgenomic	JJ
cord-253024-b393ea2u	376	24	nascent	nascent	JJ
cord-253024-b393ea2u	376	25	-	-	HYPH
cord-253024-b393ea2u	376	26	plus	plus	CC
cord-253024-b393ea2u	376	27	strands	strand	NNS
cord-253024-b393ea2u	376	28	bound	bind	VBN
cord-253024-b393ea2u	376	29	to	to	IN
cord-253024-b393ea2u	376	30	the	the	DT
cord-253024-b393ea2u	376	31	fulllength	fulllength	JJ
cord-253024-b393ea2u	376	32	negative	negative	JJ
cord-253024-b393ea2u	376	33	-	-	HYPH
cord-253024-b393ea2u	376	34	strand	strand	NN
cord-253024-b393ea2u	376	35	RNA	RNA	NNP
cord-253024-b393ea2u	376	36	(	(	-LRB-
cord-253024-b393ea2u	376	37	Baric	Baric	NNP
cord-253024-b393ea2u	376	38	et	et	NNP
cord-253024-b393ea2u	376	39	al	al	NNP
cord-253024-b393ea2u	376	40	.	.	NNP
cord-253024-b393ea2u	376	41	,	,	,
cord-253024-b393ea2u	376	42	1983	1983	CD
cord-253024-b393ea2u	376	43	)	)	-RRB-
cord-253024-b393ea2u	376	44	.	.	.
cord-253024-b393ea2u	377	1	While	while	IN
cord-253024-b393ea2u	377	2	the	the	DT
cord-253024-b393ea2u	377	3	original	original	JJ
cord-253024-b393ea2u	377	4	interpretation	interpretation	NN
cord-253024-b393ea2u	377	5	of	of	IN
cord-253024-b393ea2u	377	6	these	these	DT
cord-253024-b393ea2u	377	7	data	datum	NNS
cord-253024-b393ea2u	377	8	supported	support	VBD
cord-253024-b393ea2u	377	9	"	"	``
cord-253024-b393ea2u	377	10	leader	leader	NN
cord-253024-b393ea2u	377	11	-	-	HYPH
cord-253024-b393ea2u	377	12	primed	prime	VBN
cord-253024-b393ea2u	377	13	"	"	''
cord-253024-b393ea2u	377	14	transcription	transcription	NN
cord-253024-b393ea2u	377	15	,	,	,
cord-253024-b393ea2u	377	16	an	an	DT
cord-253024-b393ea2u	377	17	alternative	alternative	JJ
cord-253024-b393ea2u	377	18	explanation	explanation	NN
cord-253024-b393ea2u	377	19	for	for	IN
cord-253024-b393ea2u	377	20	these	these	DT
cord-253024-b393ea2u	377	21	findings	finding	NNS
cord-253024-b393ea2u	377	22	is	be	VBZ
cord-253024-b393ea2u	377	23	that	that	IN
cord-253024-b393ea2u	377	24	these	these	DT
cord-253024-b393ea2u	377	25	subgenomic	subgenomic	JJ
cord-253024-b393ea2u	377	26	nascent	nascent	JJ
cord-253024-b393ea2u	377	27	-	-	HYPH
cord-253024-b393ea2u	377	28	plus	plus	CC
cord-253024-b393ea2u	377	29	strands	strand	NNS
cord-253024-b393ea2u	377	30	have	have	VBP
cord-253024-b393ea2u	377	31	disassociated	disassociate	VBN
cord-253024-b393ea2u	377	32	from	from	IN
cord-253024-b393ea2u	377	33	subgenomic	subgenomic	JJ
cord-253024-b393ea2u	377	34	RI	RI	NNP
cord-253024-b393ea2u	377	35	RNA	RNA	NNP
cord-253024-b393ea2u	377	36	template	template	NN
cord-253024-b393ea2u	377	37	and	and	CC
cord-253024-b393ea2u	377	38	recombined	recombine	VBN
cord-253024-b393ea2u	377	39	with	with	IN
cord-253024-b393ea2u	377	40	the	the	DT
cord-253024-b393ea2u	377	41	fulllength	fulllength	JJ
cord-253024-b393ea2u	377	42	negative	negative	JJ
cord-253024-b393ea2u	377	43	-	-	HYPH
cord-253024-b393ea2u	377	44	strand	strand	NN
cord-253024-b393ea2u	377	45	RNA	RNA	NNP
cord-253024-b393ea2u	377	46	.	.	.
cord-253024-b393ea2u	378	1	Finally	finally	RB
cord-253024-b393ea2u	378	2	,	,	,
cord-253024-b393ea2u	378	3	a	a	DT
cord-253024-b393ea2u	378	4	large	large	JJ
cord-253024-b393ea2u	378	5	number	number	NN
cord-253024-b393ea2u	378	6	of	of	IN
cord-253024-b393ea2u	378	7	RNA	RNA	NNP
cord-253024-b393ea2u	378	8	recombinant	recombinant	JJ
cord-253024-b393ea2u	378	9	viruses	virus	NNS
cord-253024-b393ea2u	378	10	selected	select	VBN
cord-253024-b393ea2u	378	11	with	with	IN
cord-253024-b393ea2u	378	12	markers	marker	NNS
cord-253024-b393ea2u	378	13	in	in	IN
cord-253024-b393ea2u	378	14	the	the	DT
cord-253024-b393ea2u	378	15	S	S	NNP
cord-253024-b393ea2u	378	16	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	378	17	gene	gene	NN
cord-253024-b393ea2u	378	18	of	of	IN
cord-253024-b393ea2u	378	19	MHV	MHV	NNP
cord-253024-b393ea2u	378	20	contain	contain	VBP
cord-253024-b393ea2u	378	21	additional	additional	JJ
cord-253024-b393ea2u	378	22	crossover	crossover	NN
cord-253024-b393ea2u	378	23	sites	site	NNS
cord-253024-b393ea2u	378	24	in	in	IN
cord-253024-b393ea2u	378	25	the	the	DT
cord-253024-b393ea2u	378	26	M	M	NNP
cord-253024-b393ea2u	378	27	and	and	CC
cord-253024-b393ea2u	378	28	N	n	CD
cord-253024-b393ea2u	378	29	structural	structural	JJ
cord-253024-b393ea2u	378	30	genes	gene	NNS
cord-253024-b393ea2u	378	31	encoded	encode	VBN
cord-253024-b393ea2u	378	32	at	at	IN
cord-253024-b393ea2u	378	33	the	the	DT
cord-253024-b393ea2u	378	34	3	3	CD
cord-253024-b393ea2u	378	35	'	'	NN
cord-253024-b393ea2u	378	36	end	end	NN
cord-253024-b393ea2u	378	37	of	of	IN
cord-253024-b393ea2u	378	38	the	the	DT
cord-253024-b393ea2u	378	39	genome	genome	NN
cord-253024-b393ea2u	379	1	(	(	-LRB-
cord-253024-b393ea2u	379	2	Keck	Keck	NNP
cord-253024-b393ea2u	379	3	et	et	NNP
cord-253024-b393ea2u	379	4	al	al	NNP
cord-253024-b393ea2u	379	5	.	.	NNP
cord-253024-b393ea2u	379	6	,	,	,
cord-253024-b393ea2u	379	7	1988b	1988b	CD
cord-253024-b393ea2u	379	8	)	)	-RRB-
cord-253024-b393ea2u	379	9	.	.	.
cord-253024-b393ea2u	380	1	These	these	DT
cord-253024-b393ea2u	380	2	findings	finding	NNS
cord-253024-b393ea2u	380	3	are	be	VBP
cord-253024-b393ea2u	380	4	consistent	consistent	JJ
cord-253024-b393ea2u	380	5	with	with	IN
cord-253024-b393ea2u	380	6	the	the	DT
cord-253024-b393ea2u	380	7	idea	idea	NN
cord-253024-b393ea2u	380	8	that	that	IN
cord-253024-b393ea2u	380	9	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	380	10	minus	minus	CC
cord-253024-b393ea2u	380	11	strands	strand	NNS
cord-253024-b393ea2u	380	12	function	function	VBP
cord-253024-b393ea2u	380	13	in	in	IN
cord-253024-b393ea2u	380	14	RNA	RNA	NNP
cord-253024-b393ea2u	380	15	recombination	recombination	NN
cord-253024-b393ea2u	380	16	and	and	CC
cord-253024-b393ea2u	380	17	suggest	suggest	VBP
cord-253024-b393ea2u	380	18	that	that	IN
cord-253024-b393ea2u	380	19	recombination	recombination	NN
cord-253024-b393ea2u	380	20	events	event	NNS
cord-253024-b393ea2u	380	21	may	may	MD
cord-253024-b393ea2u	380	22	increase	increase	VB
cord-253024-b393ea2u	380	23	in	in	IN
cord-253024-b393ea2u	380	24	frequency	frequency	NN
cord-253024-b393ea2u	380	25	toward	toward	IN
cord-253024-b393ea2u	380	26	the	the	DT
cord-253024-b393ea2u	380	27	3	3	CD
cord-253024-b393ea2u	380	28	'	'	NN
cord-253024-b393ea2u	380	29	end	end	NN
cord-253024-b393ea2u	380	30	of	of	IN
cord-253024-b393ea2u	380	31	the	the	DT
cord-253024-b393ea2u	380	32	genome	genome	NN
cord-253024-b393ea2u	380	33	.	.	.
cord-253024-b393ea2u	381	1	High	high	JJ
cord-253024-b393ea2u	381	2	-	-	HYPH
cord-253024-b393ea2u	381	3	frequency	frequency	NN
cord-253024-b393ea2u	381	4	RNA	RNA	NNP
cord-253024-b393ea2u	381	5	recombination	recombination	NN
cord-253024-b393ea2u	381	6	is	be	VBZ
cord-253024-b393ea2u	381	7	a	a	DT
cord-253024-b393ea2u	381	8	unique	unique	JJ
cord-253024-b393ea2u	381	9	property	property	NN
cord-253024-b393ea2u	381	10	associated	associate	VBN
cord-253024-b393ea2u	381	11	with	with	IN
cord-253024-b393ea2u	381	12	MHV	MHV	NNP
cord-253024-b393ea2u	381	13	replication	replication	NN
cord-253024-b393ea2u	381	14	.	.	.
cord-253024-b393ea2u	382	1	Our	-PRON-	PRP$
cord-253024-b393ea2u	382	2	data	datum	NNS
cord-253024-b393ea2u	382	3	suggest	suggest	VBP
cord-253024-b393ea2u	382	4	that	that	IN
cord-253024-b393ea2u	382	5	the	the	DT
cord-253024-b393ea2u	382	6	frequency	frequency	NN
cord-253024-b393ea2u	382	7	of	of	IN
cord-253024-b393ea2u	382	8	RNA	RNA	NNP
cord-253024-b393ea2u	382	9	recombination	recombination	NN
cord-253024-b393ea2u	382	10	is	be	VBZ
cord-253024-b393ea2u	382	11	mediated	mediate	VBN
cord-253024-b393ea2u	382	12	in	in	IN
cord-253024-b393ea2u	382	13	part	part	NN
cord-253024-b393ea2u	382	14	by	by	IN
cord-253024-b393ea2u	382	15	the	the	DT
cord-253024-b393ea2u	382	16	large	large	JJ
cord-253024-b393ea2u	382	17	size	size	NN
cord-253024-b393ea2u	382	18	of	of	IN
cord-253024-b393ea2u	382	19	the	the	DT
cord-253024-b393ea2u	382	20	MHV	MHV	NNP
cord-253024-b393ea2u	382	21	genome	genome	NN
cord-253024-b393ea2u	382	22	(	(	-LRB-
cord-253024-b393ea2u	382	23	32	32	CD
cord-253024-b393ea2u	382	24	kb	kb	NNP
cord-253024-b393ea2u	382	25	)	)	-RRB-
cord-253024-b393ea2u	382	26	and	and	CC
cord-253024-b393ea2u	382	27	its	-PRON-	PRP$
cord-253024-b393ea2u	382	28	novel	novel	JJ
cord-253024-b393ea2u	382	29	mechanism	mechanism	NN
cord-253024-b393ea2u	382	30	for	for	IN
cord-253024-b393ea2u	382	31	RNA	RNA	NNP
cord-253024-b393ea2u	382	32	synthesis	synthesis	NN
cord-253024-b393ea2u	382	33	involving	involve	VBG
cord-253024-b393ea2u	382	34	"	"	``
cord-253024-b393ea2u	382	35	leader	leader	NN
cord-253024-b393ea2u	382	36	primed	prime	VBN
cord-253024-b393ea2u	382	37	"	"	``
cord-253024-b393ea2u	382	38	discontinuous	discontinuous	JJ
cord-253024-b393ea2u	382	39	transcription	transcription	NN
cord-253024-b393ea2u	382	40	and	and	CC
cord-253024-b393ea2u	382	41	mRNA	mrna	NN
cord-253024-b393ea2u	382	42	replication	replication	NN
cord-253024-b393ea2u	382	43	via	via	IN
cord-253024-b393ea2u	382	44	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	382	45	minus	minus	CC
cord-253024-b393ea2u	382	46	strands	strand	NNS
cord-253024-b393ea2u	383	1	(	(	-LRB-
cord-253024-b393ea2u	383	2	Baric	Baric	NNP
cord-253024-b393ea2u	383	3	et	et	NNP
cord-253024-b393ea2u	383	4	al	al	NNP
cord-253024-b393ea2u	383	5	.	.	.
cord-253024-b393ea2u	384	1	,	,	,
cord-253024-b393ea2u	384	2	1983	1983	CD
cord-253024-b393ea2u	384	3	(	(	-LRB-
cord-253024-b393ea2u	384	4	Baric	Baric	NNP
cord-253024-b393ea2u	384	5	et	et	NNP
cord-253024-b393ea2u	384	6	al	al	NNP
cord-253024-b393ea2u	384	7	.	.	.
cord-253024-b393ea2u	385	1	,	,	,
cord-253024-b393ea2u	385	2	,	,	,
cord-253024-b393ea2u	385	3	1987	1987	CD
cord-253024-b393ea2u	385	4	Sethna	Sethna	NNP
cord-253024-b393ea2u	385	5	et	et	NNP
cord-253024-b393ea2u	385	6	a/	a/	NNP
cord-253024-b393ea2u	385	7	,	,	,
cord-253024-b393ea2u	385	8	,	,	,
cord-253024-b393ea2u	385	9	1989	1989	CD
cord-253024-b393ea2u	385	10	Sethna	Sethna	NNP
cord-253024-b393ea2u	385	11	et	et	NNP
cord-253024-b393ea2u	385	12	a/	a/	NNP
cord-253024-b393ea2u	385	13	,	,	,
cord-253024-b393ea2u	385	14	,	,	,
cord-253024-b393ea2u	385	15	,	,	,
cord-253024-b393ea2u	386	1	1991	1991	CD
cord-253024-b393ea2u	386	2	Sawicki	Sawicki	NNP
cord-253024-b393ea2u	386	3	and	and	CC
cord-253024-b393ea2u	386	4	Sawicki	Sawicki	NNP
cord-253024-b393ea2u	386	5	,	,	,
cord-253024-b393ea2u	386	6	1990	1990	CD
cord-253024-b393ea2u	386	7	)	)	-RRB-
cord-253024-b393ea2u	386	8	.	.	.
cord-253024-b393ea2u	387	1	The	the	DT
cord-253024-b393ea2u	387	2	possibility	possibility	NN
cord-253024-b393ea2u	387	3	that	that	IN
cord-253024-b393ea2u	387	4	the	the	DT
cord-253024-b393ea2u	387	5	MHV	MHV	NNP
cord-253024-b393ea2u	387	6	recombination	recombination	NN
cord-253024-b393ea2u	387	7	rate	rate	NN
cord-253024-b393ea2u	387	8	increases	increase	VBZ
cord-253024-b393ea2u	387	9	proportionately	proportionately	RB
cord-253024-b393ea2u	387	10	from	from	IN
cord-253024-b393ea2u	387	11	the	the	DT
cord-253024-b393ea2u	387	12	5	5	CD
cord-253024-b393ea2u	387	13	'	'	''
cord-253024-b393ea2u	387	14	to	to	IN
cord-253024-b393ea2u	387	15	3	3	CD
cord-253024-b393ea2u	387	16	'	'	''
cord-253024-b393ea2u	387	17	ends	end	NNS
cord-253024-b393ea2u	387	18	of	of	IN
cord-253024-b393ea2u	387	19	the	the	DT
cord-253024-b393ea2u	387	20	genome	genome	NN
cord-253024-b393ea2u	387	21	may	may	MD
cord-253024-b393ea2u	387	22	also	also	RB
cord-253024-b393ea2u	387	23	contribute	contribute	VB
cord-253024-b393ea2u	387	24	to	to	IN
cord-253024-b393ea2u	387	25	the	the	DT
cord-253024-b393ea2u	387	26	evolution	evolution	NN
cord-253024-b393ea2u	387	27	,	,	,
cord-253024-b393ea2u	387	28	genetic	genetic	JJ
cord-253024-b393ea2u	387	29	diversity	diversity	NN
cord-253024-b393ea2u	387	30	,	,	,
cord-253024-b393ea2u	387	31	and	and	CC
cord-253024-b393ea2u	387	32	organization	organization	NN
cord-253024-b393ea2u	387	33	of	of	IN
cord-253024-b393ea2u	387	34	coronavirus	coronavirus	NN
cord-253024-b393ea2u	387	35	genomes	genome	NNS
cord-253024-b393ea2u	387	36	.	.	.
cord-253024-b393ea2u	388	1	For	for	IN
cord-253024-b393ea2u	388	2	instance	instance	NN
cord-253024-b393ea2u	388	3	,	,	,
cord-253024-b393ea2u	388	4	the	the	DT
cord-253024-b393ea2u	388	5	gene	gene	NN
cord-253024-b393ea2u	388	6	order	order	NN
cord-253024-b393ea2u	388	7	of	of	IN
cord-253024-b393ea2u	388	8	IBV	IBV	NNP
cord-253024-b393ea2u	388	9	is	be	VBZ
cord-253024-b393ea2u	388	10	different	different	JJ
cord-253024-b393ea2u	388	11	from	from	IN
cord-253024-b393ea2u	388	12	other	other	JJ
cord-253024-b393ea2u	388	13	coronaviruses	coronaviruse	NNS
cord-253024-b393ea2u	388	14	and	and	CC
cord-253024-b393ea2u	388	15	it	-PRON-	PRP
cord-253024-b393ea2u	388	16	contains	contain	VBZ
cord-253024-b393ea2u	388	17	an	an	DT
cord-253024-b393ea2u	388	18	additional	additional	JJ
cord-253024-b393ea2u	388	19	gene	gene	NN
cord-253024-b393ea2u	388	20	between	between	IN
cord-253024-b393ea2u	388	21	the	the	DT
cord-253024-b393ea2u	388	22	M	M	NNP
cord-253024-b393ea2u	388	23	and	and	CC
cord-253024-b393ea2u	388	24	N	n	CD
cord-253024-b393ea2u	388	25	genes	gene	NNS
cord-253024-b393ea2u	388	26	at	at	IN
cord-253024-b393ea2u	388	27	the	the	DT
cord-253024-b393ea2u	388	28	3'end	3'end	CD
cord-253024-b393ea2u	388	29	of	of	IN
cord-253024-b393ea2u	388	30	the	the	DT
cord-253024-b393ea2u	388	31	genome	genome	NN
cord-253024-b393ea2u	388	32	(	(	-LRB-
cord-253024-b393ea2u	388	33	Cavanagh	Cavanagh	NNP
cord-253024-b393ea2u	388	34	eta/.	eta/.	CD
cord-253024-b393ea2u	388	35	,	,	,
cord-253024-b393ea2u	388	36	1991	1991	CD
cord-253024-b393ea2u	388	37	)	)	-RRB-
cord-253024-b393ea2u	388	38	.	.	.
cord-253024-b393ea2u	389	1	TGEV	TGEV	NNP
cord-253024-b393ea2u	389	2	also	also	RB
cord-253024-b393ea2u	389	3	contains	contain	VBZ
cord-253024-b393ea2u	389	4	an	an	DT
cord-253024-b393ea2u	389	5	additional	additional	JJ
cord-253024-b393ea2u	389	6	gene	gene	NN
cord-253024-b393ea2u	389	7	,	,	,
cord-253024-b393ea2u	389	8	designated	designate	VBN
cord-253024-b393ea2u	389	9	X	X	NNP
cord-253024-b393ea2u	389	10	,	,	,
cord-253024-b393ea2u	389	11	which	which	WDT
cord-253024-b393ea2u	389	12	is	be	VBZ
cord-253024-b393ea2u	389	13	located	locate	VBN
cord-253024-b393ea2u	389	14	at	at	IN
cord-253024-b393ea2u	389	15	the	the	DT
cord-253024-b393ea2u	389	16	3'end	3'end	CD
cord-253024-b393ea2u	389	17	of	of	IN
cord-253024-b393ea2u	389	18	the	the	DT
cord-253024-b393ea2u	389	19	genome	genome	NN
cord-253024-b393ea2u	389	20	(	(	-LRB-
cord-253024-b393ea2u	389	21	Kapke	Kapke	NNP
cord-253024-b393ea2u	389	22	and	and	CC
cord-253024-b393ea2u	389	23	Brian	Brian	NNP
cord-253024-b393ea2u	389	24	,	,	,
cord-253024-b393ea2u	389	25	1986	1986	CD
cord-253024-b393ea2u	389	26	)	)	-RRB-
cord-253024-b393ea2u	389	27	.	.	.
cord-253024-b393ea2u	390	1	More	more	RBR
cord-253024-b393ea2u	390	2	remarkably	remarkably	RB
cord-253024-b393ea2u	390	3	,	,	,
cord-253024-b393ea2u	390	4	so	so	RB
cord-253024-b393ea2u	390	5	,	,	,
cord-253024-b393ea2u	390	6	me	-PRON-	PRP
cord-253024-b393ea2u	390	7	coronaviruses	coronaviruse	NNS
cord-253024-b393ea2u	390	8	,	,	,
cord-253024-b393ea2u	390	9	but	but	CC
cord-253024-b393ea2u	390	10	not	not	RB
cord-253024-b393ea2u	390	11	all	all	DT
cord-253024-b393ea2u	390	12	,	,	,
cord-253024-b393ea2u	390	13	contain	contain	VB
cord-253024-b393ea2u	390	14	a	a	DT
cord-253024-b393ea2u	390	15	p30	p30	NNP
cord-253024-b393ea2u	390	16	nonstructural	nonstructural	JJ
cord-253024-b393ea2u	390	17	protein	protein	NN
cord-253024-b393ea2u	390	18	and	and	CC
cord-253024-b393ea2u	390	19	an	an	DT
cord-253024-b393ea2u	390	20	HE	HE	NNP
cord-253024-b393ea2u	390	21	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	390	22	that	that	WDT
cord-253024-b393ea2u	390	23	is	be	VBZ
cord-253024-b393ea2u	390	24	related	relate	VBN
cord-253024-b393ea2u	390	25	to	to	IN
cord-253024-b393ea2u	390	26	the	the	DT
cord-253024-b393ea2u	390	27	influenza	influenza	NN
cord-253024-b393ea2u	390	28	C	c	NN
cord-253024-b393ea2u	390	29	virus	virus	NN
cord-253024-b393ea2u	391	1	HE	he	PRP
cord-253024-b393ea2u	391	2	(	(	-LRB-
cord-253024-b393ea2u	391	3	Luytjes	Luytjes	NNP
cord-253024-b393ea2u	391	4	et	et	NNP
cord-253024-b393ea2u	391	5	al	al	NNP
cord-253024-b393ea2u	391	6	.	.	NNP
cord-253024-b393ea2u	391	7	,	,	,
cord-253024-b393ea2u	391	8	1988	1988	CD
cord-253024-b393ea2u	391	9	)	)	-RRB-
cord-253024-b393ea2u	391	10	.	.	.
cord-253024-b393ea2u	392	1	Coronaviruses	Coronaviruses	NNP
cord-253024-b393ea2u	392	2	and	and	CC
cord-253024-b393ea2u	392	3	toroviruses	toroviruse	NNS
cord-253024-b393ea2u	392	4	may	may	MD
cord-253024-b393ea2u	392	5	also	also	RB
cord-253024-b393ea2u	392	6	have	have	VB
cord-253024-b393ea2u	392	7	diverged	diverge	VBN
cord-253024-b393ea2u	392	8	by	by	IN
cord-253024-b393ea2u	392	9	nonhomologous	nonhomologous	JJ
cord-253024-b393ea2u	392	10	recombination	recombination	NN
cord-253024-b393ea2u	392	11	events	event	NNS
cord-253024-b393ea2u	392	12	in	in	IN
cord-253024-b393ea2u	392	13	their	-PRON-	PRP$
cord-253024-b393ea2u	392	14	polymerase	polymerase	NN
cord-253024-b393ea2u	392	15	and	and	CC
cord-253024-b393ea2u	392	16	envelope	envelope	NN
cord-253024-b393ea2u	392	17	proteins	protein	NNS
cord-253024-b393ea2u	393	1	(	(	-LRB-
cord-253024-b393ea2u	393	2	Snijder	Snijder	NNP
cord-253024-b393ea2u	393	3	et	et	NNP
cord-253024-b393ea2u	393	4	al	al	NNP
cord-253024-b393ea2u	393	5	.	.	NNP
cord-253024-b393ea2u	393	6	,	,	,
cord-253024-b393ea2u	393	7	1991	1991	CD
cord-253024-b393ea2u	393	8	)	)	-RRB-
cord-253024-b393ea2u	393	9	.	.	.
cord-253024-b393ea2u	394	1	These	these	DT
cord-253024-b393ea2u	394	2	findings	finding	NNS
cord-253024-b393ea2u	394	3	are	be	VBP
cord-253024-b393ea2u	394	4	consistent	consistent	JJ
cord-253024-b393ea2u	394	5	with	with	IN
cord-253024-b393ea2u	394	6	the	the	DT
cord-253024-b393ea2u	394	7	fact	fact	NN
cord-253024-b393ea2u	394	8	that	that	IN
cord-253024-b393ea2u	394	9	the	the	DT
cord-253024-b393ea2u	394	10	coronavirus	coronavirus	NN
cord-253024-b393ea2u	394	11	genome	genome	NN
cord-253024-b393ea2u	394	12	has	have	VBZ
cord-253024-b393ea2u	394	13	a	a	DT
cord-253024-b393ea2u	394	14	remarkable	remarkable	JJ
cord-253024-b393ea2u	394	15	ability	ability	NN
cord-253024-b393ea2u	394	16	to	to	TO
cord-253024-b393ea2u	394	17	evolve	evolve	VB
cord-253024-b393ea2u	394	18	by	by	IN
cord-253024-b393ea2u	394	19	homologous	homologous	JJ
cord-253024-b393ea2u	394	20	and	and	CC
cord-253024-b393ea2u	394	21	nonhomologous	nonhomologous	JJ
cord-253024-b393ea2u	394	22	recombination	recombination	NN
cord-253024-b393ea2u	394	23	,	,	,
cord-253024-b393ea2u	394	24	especially	especially	RB
cord-253024-b393ea2u	394	25	in	in	IN
cord-253024-b393ea2u	394	26	the	the	DT
cord-253024-b393ea2u	394	27	genes	gene	NNS
cord-253024-b393ea2u	394	28	encoded	encode	VBN
cord-253024-b393ea2u	394	29	by	by	IN
cord-253024-b393ea2u	394	30	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	394	31	mRNA	mRNA	NNP
cord-253024-b393ea2u	394	32	.	.	.
cord-253024-b393ea2u	395	1	Regeneration	regeneration	NN
cord-253024-b393ea2u	395	2	of	of	IN
cord-253024-b393ea2u	395	3	a	a	DT
cord-253024-b393ea2u	395	4	functional	functional	JJ
cord-253024-b393ea2u	395	5	RNA	RNA	NNP
cord-253024-b393ea2u	395	6	virus	virus	NN
cord-253024-b393ea2u	395	7	genome	genome	NN
cord-253024-b393ea2u	395	8	by	by	IN
cord-253024-b393ea2u	395	9	recombination	recombination	NN
cord-253024-b393ea2u	395	10	between	between	IN
cord-253024-b393ea2u	395	11	deletion	deletion	NN
cord-253024-b393ea2u	395	12	mutants	mutant	NNS
cord-253024-b393ea2u	395	13	and	and	CC
cord-253024-b393ea2u	395	14	requirement	requirement	NN
cord-253024-b393ea2u	395	15	for	for	IN
cord-253024-b393ea2u	395	16	cowpea	cowpea	NNP
cord-253024-b393ea2u	395	17	chlorotic	chlorotic	JJ
cord-253024-b393ea2u	395	18	mottle	mottle	NN
cord-253024-b393ea2u	395	19	virus	virus	NN
cord-253024-b393ea2u	395	20	3a	3a	NNP
cord-253024-b393ea2u	395	21	and	and	CC
cord-253024-b393ea2u	395	22	coat	coat	NN
cord-253024-b393ea2u	395	23	genes	gene	NNS
cord-253024-b393ea2u	395	24	for	for	IN
cord-253024-b393ea2u	395	25	systemic	systemic	JJ
cord-253024-b393ea2u	395	26	infection	infection	NN
cord-253024-b393ea2u	395	27	Identification	identification	NN
cord-253024-b393ea2u	395	28	of	of	IN
cord-253024-b393ea2u	395	29	a	a	DT
cord-253024-b393ea2u	395	30	domain	domain	NN
cord-253024-b393ea2u	395	31	required	require	VBN
cord-253024-b393ea2u	395	32	for	for	IN
cord-253024-b393ea2u	395	33	autoproteolytic	autoproteolytic	JJ
cord-253024-b393ea2u	395	34	cleavage	cleavage	NN
cord-253024-b393ea2u	395	35	of	of	IN
cord-253024-b393ea2u	395	36	murine	murine	JJ
cord-253024-b393ea2u	395	37	coronavirus	coronavirus	NN
cord-253024-b393ea2u	395	38	gene	gene	NN
cord-253024-b393ea2u	395	39	A	a	DT
cord-253024-b393ea2u	395	40	polyprotein	polyprotein	NN
cord-253024-b393ea2u	396	1	An	an	DT
cord-253024-b393ea2u	396	2	in	in	FW
cord-253024-b393ea2u	396	3	vitro	vitro	FW
cord-253024-b393ea2u	396	4	system	system	NN
cord-253024-b393ea2u	396	5	for	for	IN
cord-253024-b393ea2u	396	6	the	the	DT
cord-253024-b393ea2u	396	7	leader	leader	NN
cord-253024-b393ea2u	396	8	-	-	HYPH
cord-253024-b393ea2u	396	9	primed	prime	VBN
cord-253024-b393ea2u	396	10	transcription	transcription	NN
cord-253024-b393ea2u	396	11	of	of	IN
cord-253024-b393ea2u	396	12	coronavirus	coronavirus	NN
cord-253024-b393ea2u	396	13	mRNAs	mrnas	ADD
cord-253024-b393ea2u	396	14	.	.	.
cord-253024-b393ea2u	397	1	f/&Y0	f/&Y0	NNP
cord-253024-b393ea2u	398	1	A	a	DT
cord-253024-b393ea2u	398	2	clustering	clustering	NN
cord-253024-b393ea2u	398	3	of	of	IN
cord-253024-b393ea2u	398	4	RNA	RNA	NNP
cord-253024-b393ea2u	398	5	recombination	recombination	NN
cord-253024-b393ea2u	398	6	sites	site	NNS
cord-253024-b393ea2u	398	7	adjacent	adjacent	JJ
cord-253024-b393ea2u	398	8	to	to	IN
cord-253024-b393ea2u	398	9	a	a	DT
cord-253024-b393ea2u	398	10	hypervariable	hypervariable	JJ
cord-253024-b393ea2u	398	11	region	region	NN
cord-253024-b393ea2u	398	12	of	of	IN
cord-253024-b393ea2u	398	13	the	the	DT
cord-253024-b393ea2u	398	14	peplomer	peplomer	JJ
cord-253024-b393ea2u	398	15	gene	gene	NN
cord-253024-b393ea2u	398	16	of	of	IN
cord-253024-b393ea2u	398	17	murine	murine	JJ
cord-253024-b393ea2u	398	18	coronavirus	coronavirus	NN
cord-253024-b393ea2u	398	19	Random	random	JJ
cord-253024-b393ea2u	398	20	nature	nature	NN
cord-253024-b393ea2u	398	21	of	of	IN
cord-253024-b393ea2u	398	22	coronavirus	coronavirus	NN
cord-253024-b393ea2u	398	23	RNA	RNA	NNP
cord-253024-b393ea2u	398	24	recombination	recombination	NN
cord-253024-b393ea2u	398	25	the	the	DT
cord-253024-b393ea2u	398	26	absence	absence	NN
cord-253024-b393ea2u	398	27	of	of	IN
cord-253024-b393ea2u	398	28	selection	selection	NN
cord-253024-b393ea2u	398	29	pressure	pressure	NN
cord-253024-b393ea2u	398	30	Characterization	characterization	NN
cord-253024-b393ea2u	398	31	of	of	IN
cord-253024-b393ea2u	398	32	replicative	replicative	JJ
cord-253024-b393ea2u	398	33	intermediate	intermediate	JJ
cord-253024-b393ea2u	398	34	RNA	RNA	NNP
cord-253024-b393ea2u	398	35	of	of	IN
cord-253024-b393ea2u	398	36	mouse	mouse	NNP
cord-253024-b393ea2u	398	37	hepatitis	hepatitis	NNP
cord-253024-b393ea2u	398	38	virus	virus	NN
cord-253024-b393ea2u	398	39	:	:	:
cord-253024-b393ea2u	398	40	Presence	presence	NN
cord-253024-b393ea2u	398	41	of	of	IN
cord-253024-b393ea2u	398	42	leader	leader	NN
cord-253024-b393ea2u	398	43	RNA	RNA	NNP
cord-253024-b393ea2u	398	44	sequences	sequence	NNS
cord-253024-b393ea2u	398	45	on	on	IN
cord-253024-b393ea2u	398	46	nascent	nascent	JJ
cord-253024-b393ea2u	398	47	chains	chain	NNS
cord-253024-b393ea2u	398	48	Characterization	characterization	NN
cord-253024-b393ea2u	398	49	of	of	IN
cord-253024-b393ea2u	398	50	leader	leader	NN
cord-253024-b393ea2u	398	51	-	-	HYPH
cord-253024-b393ea2u	398	52	related	relate	VBN
cord-253024-b393ea2u	398	53	small	small	JJ
cord-253024-b393ea2u	398	54	RNAs	rna	NNS
cord-253024-b393ea2u	398	55	in	in	IN
cord-253024-b393ea2u	398	56	coronavirus	coronavirus	NN
cord-253024-b393ea2u	398	57	-	-	HYPH
cord-253024-b393ea2u	398	58	infected	infect	VBN
cord-253024-b393ea2u	398	59	cells	cell	NNS
cord-253024-b393ea2u	398	60	:	:	:
cord-253024-b393ea2u	399	1	Further	further	JJ
cord-253024-b393ea2u	399	2	evidence	evidence	NN
cord-253024-b393ea2u	399	3	for	for	IN
cord-253024-b393ea2u	399	4	leader	leader	NN
cord-253024-b393ea2u	399	5	-	-	HYPH
cord-253024-b393ea2u	399	6	primed	prime	VBN
cord-253024-b393ea2u	399	7	mechanism	mechanism	NN
cord-253024-b393ea2u	399	8	of	of	IN
cord-253024-b393ea2u	399	9	transcription	transcription	NN
cord-253024-b393ea2u	399	10	Analysis	Analysis	NNP
cord-253024-b393ea2u	399	11	of	of	IN
cord-253024-b393ea2u	399	12	intercellular	intercellular	JJ
cord-253024-b393ea2u	399	13	small	small	JJ
cord-253024-b393ea2u	399	14	RNAs	rna	NNS
cord-253024-b393ea2u	399	15	of	of	IN
cord-253024-b393ea2u	399	16	mouse	mouse	NNP
cord-253024-b393ea2u	399	17	hepatitis	hepatitis	NNP
cord-253024-b393ea2u	399	18	virus	virus	NN
cord-253024-b393ea2u	399	19	:	:	:
cord-253024-b393ea2u	399	20	Evidence	evidence	NN
cord-253024-b393ea2u	399	21	for	for	IN
cord-253024-b393ea2u	399	22	discontinuous	discontinuous	JJ
cord-253024-b393ea2u	399	23	transcription	transcription	NN
cord-253024-b393ea2u	399	24	Interactions	interaction	NNS
cord-253024-b393ea2u	399	25	between	between	IN
cord-253024-b393ea2u	399	26	coronavirus	coronavirus	NN
cord-253024-b393ea2u	399	27	nucleocapsid	nucleocapsid	NN
cord-253024-b393ea2u	399	28	protein	protein	NN
cord-253024-b393ea2u	399	29	and	and	CC
cord-253024-b393ea2u	399	30	viral	viral	JJ
cord-253024-b393ea2u	399	31	RNAs	rna	NNS
cord-253024-b393ea2u	399	32	:	:	:
cord-253024-b393ea2u	399	33	Implications	implication	NNS
cord-253024-b393ea2u	399	34	for	for	IN
cord-253024-b393ea2u	399	35	viral	viral	JJ
cord-253024-b393ea2u	399	36	transcription	transcription	NN
cord-253024-b393ea2u	399	37	Establishing	establish	VBG
cord-253024-b393ea2u	399	38	a	a	DT
cord-253024-b393ea2u	399	39	genetic	genetic	JJ
cord-253024-b393ea2u	399	40	recombination	recombination	NN
cord-253024-b393ea2u	399	41	map	map	NN
cord-253024-b393ea2u	399	42	for	for	IN
cord-253024-b393ea2u	399	43	MHV	MHV	NNP
cord-253024-b393ea2u	399	44	-	-	HYPH
cord-253024-b393ea2u	399	45	A59	A59	NNP
cord-253024-b393ea2u	399	46	complementation	complementation	NN
cord-253024-b393ea2u	399	47	groups	group	NNS
cord-253024-b393ea2u	399	48	Nucleotide	nucleotide	JJ
cord-253024-b393ea2u	399	49	sequence	sequence	NN
cord-253024-b393ea2u	399	50	of	of	IN
cord-253024-b393ea2u	399	51	the	the	DT
cord-253024-b393ea2u	399	52	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	399	53	S	s	NN
cord-253024-b393ea2u	399	54	gene	gene	NN
cord-253024-b393ea2u	399	55	of	of	IN
cord-253024-b393ea2u	399	56	bovine	bovine	JJ
cord-253024-b393ea2u	399	57	enteric	enteric	JJ
cord-253024-b393ea2u	399	58	coronavirus	coronavirus	NN
cord-253024-b393ea2u	399	59	and	and	CC
cord-253024-b393ea2u	399	60	comparison	comparison	NN
cord-253024-b393ea2u	399	61	with	with	IN
cord-253024-b393ea2u	399	62	the	the	DT
cord-253024-b393ea2u	399	63	S	S	NNP
cord-253024-b393ea2u	399	64	proteins	protein	NNS
cord-253024-b393ea2u	399	65	of	of	IN
cord-253024-b393ea2u	399	66	two	two	CD
cord-253024-b393ea2u	399	67	mouse	mouse	NN
cord-253024-b393ea2u	399	68	hepatitis	hepatitis	NN
cord-253024-b393ea2u	399	69	virus	virus	NN
cord-253024-b393ea2u	400	1	strains	strain	NNS
cord-253024-b393ea2u	400	2	Characterization	characterization	NN
cord-253024-b393ea2u	400	3	of	of	IN
cord-253024-b393ea2u	400	4	two	two	CD
cord-253024-b393ea2u	400	5	RNA	RNA	NNP
cord-253024-b393ea2u	400	6	polymerase	polymerase	NN
cord-253024-b393ea2u	400	7	activities	activity	NNS
cord-253024-b393ea2u	400	8	induced	induce	VBN
cord-253024-b393ea2u	400	9	by	by	IN
cord-253024-b393ea2u	400	10	mouse	mouse	NNP
cord-253024-b393ea2u	400	11	hepatitis	hepatitis	NNP
cord-253024-b393ea2u	400	12	virus	virus	NN
cord-253024-b393ea2u	401	1	Further	further	JJ
cord-253024-b393ea2u	401	2	characterization	characterization	NN
cord-253024-b393ea2u	401	3	of	of	IN
cord-253024-b393ea2u	401	4	mouse	mouse	NN
cord-253024-b393ea2u	401	5	hepatitis	hepatitis	NNP
cord-253024-b393ea2u	401	6	virus	virus	NN
cord-253024-b393ea2u	401	7	RNA	RNA	NNP
cord-253024-b393ea2u	401	8	-	-	HYPH
cord-253024-b393ea2u	401	9	dependent	dependent	JJ
cord-253024-b393ea2u	401	10	RNA	RNA	NNP
cord-253024-b393ea2u	401	11	polymerases	polymerase	NNS
cord-253024-b393ea2u	402	1	The	the	DT
cord-253024-b393ea2u	402	2	primary	primary	JJ
cord-253024-b393ea2u	402	3	structure	structure	NN
cord-253024-b393ea2u	402	4	and	and	CC
cord-253024-b393ea2u	402	5	expression	expression	NN
cord-253024-b393ea2u	402	6	of	of	IN
cord-253024-b393ea2u	402	7	the	the	DT
cord-253024-b393ea2u	402	8	second	second	JJ
cord-253024-b393ea2u	402	9	open	open	JJ
cord-253024-b393ea2u	402	10	reading	reading	NN
cord-253024-b393ea2u	402	11	frame	frame	NN
cord-253024-b393ea2u	402	12	of	of	IN
cord-253024-b393ea2u	402	13	the	the	DT
cord-253024-b393ea2u	402	14	polymerase	polymerase	NN
cord-253024-b393ea2u	402	15	gene	gene	NN
cord-253024-b393ea2u	402	16	of	of	IN
cord-253024-b393ea2u	402	17	the	the	DT
cord-253024-b393ea2u	402	18	coronavirus	coronavirus	NN
cord-253024-b393ea2u	402	19	MHV	MHV	NNP
cord-253024-b393ea2u	402	20	-	-	HYPH
cord-253024-b393ea2u	402	21	A59	A59	NNP
cord-253024-b393ea2u	402	22	;	;	:
cord-253024-b393ea2u	402	23	a	a	DT
cord-253024-b393ea2u	402	24	highly	highly	RB
cord-253024-b393ea2u	402	25	consetved	consetved	JJ
cord-253024-b393ea2u	402	26	polymerase	polymerase	NN
cord-253024-b393ea2u	402	27	is	be	VBZ
cord-253024-b393ea2u	402	28	expressed	express	VBN
cord-253024-b393ea2u	402	29	by	by	IN
cord-253024-b393ea2u	402	30	an	an	DT
cord-253024-b393ea2u	402	31	efficient	efficient	JJ
cord-253024-b393ea2u	402	32	ribosomal	ribosomal	JJ
cord-253024-b393ea2u	402	33	frameshifting	frameshifte	VBG
cord-253024-b393ea2u	402	34	mechanism	mechanism	NN
cord-253024-b393ea2u	402	35	Genetic	genetic	JJ
cord-253024-b393ea2u	402	36	recombination	recombination	NN
cord-253024-b393ea2u	402	37	between	between	IN
cord-253024-b393ea2u	402	38	RNA	RNA	NNP
cord-253024-b393ea2u	402	39	components	component	NNS
cord-253024-b393ea2u	402	40	of	of	IN
cord-253024-b393ea2u	402	41	a	a	DT
cord-253024-b393ea2u	402	42	multipartite	multipartite	NNP
cord-253024-b393ea2u	402	43	plant	plant	NN
cord-253024-b393ea2u	402	44	virus	virus	NN
cord-253024-b393ea2u	402	45	Molecular	molecular	JJ
cord-253024-b393ea2u	402	46	basis	basis	NN
cord-253024-b393ea2u	402	47	of	of	IN
cord-253024-b393ea2u	402	48	the	the	DT
cord-253024-b393ea2u	402	49	variation	variation	NN
cord-253024-b393ea2u	402	50	exhibited	exhibit	VBN
cord-253024-b393ea2u	402	51	by	by	IN
cord-253024-b393ea2u	402	52	avian	avian	JJ
cord-253024-b393ea2u	402	53	infectious	infectious	JJ
cord-253024-b393ea2u	402	54	bronchitis	bronchitis	NN
cord-253024-b393ea2u	402	55	coronavirus	coronavirus	NN
cord-253024-b393ea2u	402	56	(	(	-LRB-
cord-253024-b393ea2u	402	57	IBV	IBV	NNP
cord-253024-b393ea2u	402	58	)	)	-RRB-
cord-253024-b393ea2u	402	59	High	high	JJ
cord-253024-b393ea2u	402	60	negative	negative	JJ
cord-253024-b393ea2u	402	61	interference	interference	NN
cord-253024-b393ea2u	402	62	over	over	IN
cord-253024-b393ea2u	402	63	short	short	JJ
cord-253024-b393ea2u	402	64	segments	segment	NNS
cord-253024-b393ea2u	402	65	of	of	IN
cord-253024-b393ea2u	402	66	the	the	DT
cord-253024-b393ea2u	402	67	genetic	genetic	JJ
cord-253024-b393ea2u	402	68	structure	structure	NN
cord-253024-b393ea2u	402	69	of	of	IN
cord-253024-b393ea2u	402	70	bacteriophage	bacteriophage	NN
cord-253024-b393ea2u	402	71	T4	T4	NNP
cord-253024-b393ea2u	402	72	Monoclonal	Monoclonal	NNP
cord-253024-b393ea2u	402	73	antibodies	antibody	NNS
cord-253024-b393ea2u	402	74	to	to	IN
cord-253024-b393ea2u	402	75	murine	murine	JJ
cord-253024-b393ea2u	402	76	hepatitis	hepatitis	NN
cord-253024-b393ea2u	402	77	virus-4	virus-4	NNP
cord-253024-b393ea2u	403	1	(	(	-LRB-
cord-253024-b393ea2u	403	2	strain	strain	NN
cord-253024-b393ea2u	403	3	JHM	JHM	NNP
cord-253024-b393ea2u	403	4	)	)	-RRB-
cord-253024-b393ea2u	403	5	define	define	VB
cord-253024-b393ea2u	403	6	the	the	DT
cord-253024-b393ea2u	403	7	viral	viral	JJ
cord-253024-b393ea2u	403	8	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	403	9	responsible	responsible	JJ
cord-253024-b393ea2u	403	10	for	for	IN
cord-253024-b393ea2u	403	11	attachment	attachment	NN
cord-253024-b393ea2u	403	12	and	and	CC
cord-253024-b393ea2u	403	13	cell	cell	NN
cord-253024-b393ea2u	403	14	-	-	HYPH
cord-253024-b393ea2u	403	15	cell	cell	NN
cord-253024-b393ea2u	403	16	fusion	fusion	NN
cord-253024-b393ea2u	404	1	ln	ln	NNP
cord-253024-b393ea2u	404	2	vitro	vitro	FW
cord-253024-b393ea2u	404	3	replication	replication	NN
cord-253024-b393ea2u	404	4	of	of	IN
cord-253024-b393ea2u	404	5	mouse	mouse	NN
cord-253024-b393ea2u	404	6	hepatitis	hepatitis	NN
cord-253024-b393ea2u	404	7	virus	virus	NN
cord-253024-b393ea2u	404	8	strain	strain	VBP
cord-253024-b393ea2u	404	9	A59	A59	NNP
cord-253024-b393ea2u	404	10	A	a	DT
cord-253024-b393ea2u	404	11	genetic	genetic	JJ
cord-253024-b393ea2u	404	12	map	map	NN
cord-253024-b393ea2u	404	13	of	of	IN
cord-253024-b393ea2u	404	14	poliovirus	poliovirus	NN
cord-253024-b393ea2u	404	15	temperature	temperature	NN
cord-253024-b393ea2u	404	16	-	-	HYPH
cord-253024-b393ea2u	404	17	sensitive	sensitive	JJ
cord-253024-b393ea2u	404	18	mutants	mutant	NNS
cord-253024-b393ea2u	404	19	Attempts	attempt	VBZ
cord-253024-b393ea2u	404	20	to	to	TO
cord-253024-b393ea2u	404	21	extend	extend	VB
cord-253024-b393ea2u	404	22	the	the	DT
cord-253024-b393ea2u	404	23	genetic	genetic	JJ
cord-253024-b393ea2u	404	24	map	map	NN
cord-253024-b393ea2u	404	25	of	of	IN
cord-253024-b393ea2u	404	26	poliovirus	poliovirus	NN
cord-253024-b393ea2u	404	27	temperature	temperature	NN
cord-253024-b393ea2u	404	28	-	-	HYPH
cord-253024-b393ea2u	404	29	sensitive	sensitive	JJ
cord-253024-b393ea2u	404	30	mutants	mutant	NNS
cord-253024-b393ea2u	404	31	Genetics	Genetics	NNPS
cord-253024-b393ea2u	404	32	of	of	IN
cord-253024-b393ea2u	404	33	picornaviruses	picornaviruses	NNP
cord-253024-b393ea2u	404	34	Pathogenicity	Pathogenicity	NNP
cord-253024-b393ea2u	404	35	of	of	IN
cord-253024-b393ea2u	404	36	antigenic	antigenic	JJ
cord-253024-b393ea2u	404	37	variants	variant	NNS
cord-253024-b393ea2u	404	38	of	of	IN
cord-253024-b393ea2u	404	39	murine	murine	JJ
cord-253024-b393ea2u	404	40	coronavirus	coronavirus	NN
cord-253024-b393ea2u	404	41	JHM	JHM	NNP
cord-253024-b393ea2u	404	42	selected	select	VBN
cord-253024-b393ea2u	404	43	with	with	IN
cord-253024-b393ea2u	404	44	monoclonal	monoclonal	JJ
cord-253024-b393ea2u	404	45	antibodies	antibody	NNS
cord-253024-b393ea2u	404	46	Proteolytic	proteolytic	JJ
cord-253024-b393ea2u	404	47	cleavage	cleavage	NN
cord-253024-b393ea2u	404	48	of	of	IN
cord-253024-b393ea2u	404	49	the	the	DT
cord-253024-b393ea2u	404	50	E2	E2	NNP
cord-253024-b393ea2u	404	51	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	404	52	of	of	IN
cord-253024-b393ea2u	404	53	murine	murine	JJ
cord-253024-b393ea2u	404	54	coronavirus	coronavirus	NN
cord-253024-b393ea2u	404	55	:	:	:
cord-253024-b393ea2u	404	56	Host	host	NN
cord-253024-b393ea2u	404	57	dependent	dependent	JJ
cord-253024-b393ea2u	404	58	differences	difference	NNS
cord-253024-b393ea2u	404	59	in	in	IN
cord-253024-b393ea2u	404	60	proteolytic	proteolytic	JJ
cord-253024-b393ea2u	404	61	cleavage	cleavage	NN
cord-253024-b393ea2u	404	62	and	and	CC
cord-253024-b393ea2u	404	63	cell	cell	NN
cord-253024-b393ea2u	404	64	fusion	fusion	NN
cord-253024-b393ea2u	404	65	.	.	.
cord-253024-b393ea2u	405	1	1	1	LS
cord-253024-b393ea2u	405	2	.	.	.
cord-253024-b393ea2u	406	1	Viral	Viral	NNP
cord-253024-b393ea2u	406	2	Alteratlon	Alteratlon	NNP
cord-253024-b393ea2u	406	3	of	of	IN
cord-253024-b393ea2u	406	4	the	the	DT
cord-253024-b393ea2u	406	5	pH	pH	NNP
cord-253024-b393ea2u	406	6	dependence	dependence	NN
cord-253024-b393ea2u	406	7	of	of	IN
cord-253024-b393ea2u	406	8	coronavirus	coronavirus	NN
cord-253024-b393ea2u	406	9	-	-	HYPH
cord-253024-b393ea2u	406	10	induced	induce	VBN
cord-253024-b393ea2u	406	11	cell	cell	NN
cord-253024-b393ea2u	406	12	fusion	fusion	NN
cord-253024-b393ea2u	406	13	:	:	:
cord-253024-b393ea2u	407	1	Effect	effect	NN
cord-253024-b393ea2u	407	2	of	of	IN
cord-253024-b393ea2u	407	3	mutations	mutation	NNS
cord-253024-b393ea2u	407	4	in	in	IN
cord-253024-b393ea2u	407	5	the	the	DT
cord-253024-b393ea2u	407	6	spike	spike	NN
cord-253024-b393ea2u	407	7	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	407	8	.	.	.
cord-253024-b393ea2u	408	1	1	1	LS
cord-253024-b393ea2u	408	2	.	.	.
cord-253024-b393ea2u	409	1	Viral	Viral	NNP
cord-253024-b393ea2u	409	2	Escherichia	Escherichia	NNP
cord-253024-b393ea2u	409	3	co	co	NN
cord-253024-b393ea2u	409	4	/	/	SYM
cord-253024-b393ea2u	409	5	i	i	PRP
cord-253024-b393ea2u	409	6	mutator	mutator	NN
cord-253024-b393ea2u	409	7	mutants	mutant	NNS
cord-253024-b393ea2u	409	8	deficient	deficient	JJ
cord-253024-b393ea2u	409	9	in	in	IN
cord-253024-b393ea2u	409	10	methylation	methylation	NN
cord-253024-b393ea2u	409	11	-	-	HYPH
cord-253024-b393ea2u	409	12	Instructed	instruct	VBN
cord-253024-b393ea2u	409	13	DNA	dna	NN
cord-253024-b393ea2u	409	14	mismatch	mismatch	NN
cord-253024-b393ea2u	409	15	correction	correction	NN
cord-253024-b393ea2u	410	1	Western	western	JJ
cord-253024-b393ea2u	410	2	equine	equine	JJ
cord-253024-b393ea2u	410	3	encephalitis	encephalitis	NN
cord-253024-b393ea2u	410	4	virus	virus	NN
cord-253024-b393ea2u	410	5	is	be	VBZ
cord-253024-b393ea2u	410	6	a	a	DT
cord-253024-b393ea2u	410	7	recombinant	recombinant	JJ
cord-253024-b393ea2u	410	8	virus	virus	NN
cord-253024-b393ea2u	410	9	Mapping	Mapping	NNP
cord-253024-b393ea2u	410	10	RNA	rna	NN
cord-253024-b393ea2u	410	11	-	-	HYPH
cord-253024-b393ea2u	410	12	temperature	temperature	NN
cord-253024-b393ea2u	410	13	sensitive	sensitive	JJ
cord-253024-b393ea2u	410	14	mutants	mutant	NNS
cord-253024-b393ea2u	410	15	of	of	IN
cord-253024-b393ea2u	410	16	slndbls	slndbls	JJ
cord-253024-b393ea2u	410	17	virus	virus	NN
cord-253024-b393ea2u	410	18	:	:	:
cord-253024-b393ea2u	410	19	Complementation	Complementation	NNP
cord-253024-b393ea2u	410	20	group	group	NN
cord-253024-b393ea2u	410	21	F	F	NNP
cord-253024-b393ea2u	410	22	mutants	mutant	NNS
cord-253024-b393ea2u	410	23	have	have	VBP
cord-253024-b393ea2u	410	24	lesions	lesion	NNS
cord-253024-b393ea2u	410	25	in	in	IN
cord-253024-b393ea2u	410	26	NSP4	NSP4	NNP
cord-253024-b393ea2u	410	27	Mapping	Mapping	NNP
cord-253024-b393ea2u	410	28	of	of	IN
cord-253024-b393ea2u	410	29	RNA	RNA	NNP
cord-253024-b393ea2u	410	30	-	-	HYPH
cord-253024-b393ea2u	410	31	temperature	temperature	NN
cord-253024-b393ea2u	410	32	sensitive	sensitive	JJ
cord-253024-b393ea2u	410	33	mutants	mutant	NNS
cord-253024-b393ea2u	410	34	of	of	IN
cord-253024-b393ea2u	410	35	Sindbis	Sindbis	NNP
cord-253024-b393ea2u	410	36	virus	virus	NN
cord-253024-b393ea2u	410	37	:	:	:
cord-253024-b393ea2u	410	38	Assignment	assignment	NN
cord-253024-b393ea2u	410	39	of	of	IN
cord-253024-b393ea2u	410	40	complementatlon	complementatlon	NNP
cord-253024-b393ea2u	410	41	groups	group	NNS
cord-253024-b393ea2u	410	42	A	A	NNP
cord-253024-b393ea2u	410	43	,	,	,
cord-253024-b393ea2u	410	44	B	b	NN
cord-253024-b393ea2u	410	45	,	,	,
cord-253024-b393ea2u	410	46	and	and	CC
cord-253024-b393ea2u	410	47	G	g	NN
cord-253024-b393ea2u	410	48	to	to	IN
cord-253024-b393ea2u	410	49	nonstructural	nonstructural	JJ
cord-253024-b393ea2u	410	50	proteins	protein	NNS
cord-253024-b393ea2u	411	1	Defective	defective	JJ
cord-253024-b393ea2u	411	2	lnterfenng	lnterfenng	JJ
cord-253024-b393ea2u	411	3	rhabdoviruses	rhabdovirus	NNS
cord-253024-b393ea2u	411	4	Sequence	Sequence	NNP
cord-253024-b393ea2u	411	5	analysis	analysis	NN
cord-253024-b393ea2u	411	6	of	of	IN
cord-253024-b393ea2u	411	7	the	the	DT
cord-253024-b393ea2u	411	8	parclne	parclne	JJ
cord-253024-b393ea2u	411	9	transmissible	transmissible	JJ
cord-253024-b393ea2u	411	10	gastroenteritis	gastroenteritis	NN
cord-253024-b393ea2u	411	11	coronavirus	coronavirus	NN
cord-253024-b393ea2u	411	12	nucleocapsid	nucleocapsid	NN
cord-253024-b393ea2u	411	13	protein	protein	NN
cord-253024-b393ea2u	411	14	gene	gene	NN
cord-253024-b393ea2u	411	15	Multiple	multiple	JJ
cord-253024-b393ea2u	411	16	recomblnatlon	recomblnatlon	NN
cord-253024-b393ea2u	411	17	sites	site	NNS
cord-253024-b393ea2u	411	18	at	at	IN
cord-253024-b393ea2u	411	19	the	the	DT
cord-253024-b393ea2u	411	20	5	5	CD
cord-253024-b393ea2u	411	21	'	'	NN
cord-253024-b393ea2u	411	22	end	end	NN
cord-253024-b393ea2u	411	23	of	of	IN
cord-253024-b393ea2u	411	24	the	the	DT
cord-253024-b393ea2u	411	25	murine	murine	JJ
cord-253024-b393ea2u	411	26	coronavirus	coronavirus	NN
cord-253024-b393ea2u	411	27	RNA	RNA	NNP
cord-253024-b393ea2u	412	1	In	in	IN
cord-253024-b393ea2u	412	2	viva	viva	NNP
cord-253024-b393ea2u	412	3	RNA	RNA	NNP
cord-253024-b393ea2u	412	4	-	-	HYPH
cord-253024-b393ea2u	412	5	RNA	RNA	NNP
cord-253024-b393ea2u	412	6	recomblnatlon	recomblnatlon	NN
cord-253024-b393ea2u	412	7	of	of	IN
cord-253024-b393ea2u	412	8	coronavlrus	coronavlrus	NNP
cord-253024-b393ea2u	413	1	In	in	IN
cord-253024-b393ea2u	413	2	mouse	mouse	NN
cord-253024-b393ea2u	413	3	brain	brain	NN
cord-253024-b393ea2u	413	4	RNA	RNA	NNP
cord-253024-b393ea2u	413	5	recombination	recombination	NN
cord-253024-b393ea2u	413	6	of	of	IN
cord-253024-b393ea2u	413	7	munne	munne	NNP
cord-253024-b393ea2u	413	8	coronaviruses	coronaviruse	NNS
cord-253024-b393ea2u	413	9	:	:	:
cord-253024-b393ea2u	413	10	Recomblnatlon	Recomblnatlon	NNP
cord-253024-b393ea2u	413	11	between	between	IN
cord-253024-b393ea2u	413	12	fusion	fusion	NN
cord-253024-b393ea2u	413	13	-	-	HYPH
cord-253024-b393ea2u	413	14	positive	positive	JJ
cord-253024-b393ea2u	413	15	mouse	mouse	NN
cord-253024-b393ea2u	413	16	hepatitis	hepatitis	NN
cord-253024-b393ea2u	413	17	virus	virus	NN
cord-253024-b393ea2u	413	18	A59	A59	NNP
cord-253024-b393ea2u	413	19	and	and	CC
cord-253024-b393ea2u	413	20	fusion	fusion	NN
cord-253024-b393ea2u	413	21	-	-	HYPH
cord-253024-b393ea2u	413	22	negatjve	negatjve	JJ
cord-253024-b393ea2u	413	23	mouse	mouse	NN
cord-253024-b393ea2u	413	24	hepatitis	hepatitis	NNP
cord-253024-b393ea2u	413	25	virus	virus	NN
cord-253024-b393ea2u	413	26	2	2	CD
cord-253024-b393ea2u	413	27	Multiple	multiple	JJ
cord-253024-b393ea2u	413	28	sites	site	NNS
cord-253024-b393ea2u	413	29	of	of	IN
cord-253024-b393ea2u	413	30	recombination	recombination	NN
cord-253024-b393ea2u	413	31	within	within	IN
cord-253024-b393ea2u	413	32	the	the	DT
cord-253024-b393ea2u	413	33	RNA	RNA	NNP
cord-253024-b393ea2u	413	34	genome	genome	NN
cord-253024-b393ea2u	413	35	of	of	IN
cord-253024-b393ea2u	413	36	foot	foot	NN
cord-253024-b393ea2u	413	37	-	-	HYPH
cord-253024-b393ea2u	413	38	and	and	CC
cord-253024-b393ea2u	413	39	-	-	HYPH
cord-253024-b393ea2u	413	40	mouth	mouth	NN
cord-253024-b393ea2u	413	41	disease	disease	NN
cord-253024-b393ea2u	413	42	virus	virus	NN
cord-253024-b393ea2u	413	43	Genetic	genetic	JJ
cord-253024-b393ea2u	413	44	recombination	recombination	NN
cord-253024-b393ea2u	413	45	In	in	IN
cord-253024-b393ea2u	413	46	RNA	RNA	NNP
cord-253024-b393ea2u	413	47	viruses	virus	NNS
cord-253024-b393ea2u	414	1	Preferred	preferred	JJ
cord-253024-b393ea2u	414	2	sites	site	NNS
cord-253024-b393ea2u	414	3	of	of	IN
cord-253024-b393ea2u	414	4	recombination	recombination	NN
cord-253024-b393ea2u	414	5	in	in	IN
cord-253024-b393ea2u	414	6	pollovirus	pollovirus	NNP
cord-253024-b393ea2u	414	7	RNA	RNA	NNP
cord-253024-b393ea2u	414	8	:	:	:
cord-253024-b393ea2u	415	1	An	an	DT
cord-253024-b393ea2u	415	2	analysis	analysis	NN
cord-253024-b393ea2u	415	3	of	of	IN
cord-253024-b393ea2u	415	4	40	40	CD
cord-253024-b393ea2u	415	5	intertypic	intertypic	NNP
cord-253024-b393ea2u	415	6	cross	cross	NNP
cord-253024-b393ea2u	415	7	-	-	HYPH
cord-253024-b393ea2u	415	8	over	over	RP
cord-253024-b393ea2u	415	9	sequences	sequence	NNS
cord-253024-b393ea2u	416	1	The	the	DT
cord-253024-b393ea2u	416	2	mechanism	mechanism	NN
cord-253024-b393ea2u	416	3	of	of	IN
cord-253024-b393ea2u	416	4	RNA	RNA	NNP
cord-253024-b393ea2u	416	5	recombination	recombination	NN
cord-253024-b393ea2u	417	1	In	in	IN
cord-253024-b393ea2u	418	1	pollovirus	pollovirus	NNP
cord-253024-b393ea2u	418	2	Temperature	temperature	NN
cord-253024-b393ea2u	418	3	-	-	HYPH
cord-253024-b393ea2u	418	4	sensitive	sensitive	JJ
cord-253024-b393ea2u	418	5	mutants	mutant	NNS
cord-253024-b393ea2u	418	6	of	of	IN
cord-253024-b393ea2u	418	7	mouse	mouse	NN
cord-253024-b393ea2u	418	8	hepatitis	hepatitis	NN
cord-253024-b393ea2u	418	9	virus	virus	NN
cord-253024-b393ea2u	418	10	strain	strain	VBP
cord-253024-b393ea2u	418	11	A59	A59	NNP
cord-253024-b393ea2u	418	12	:	:	:
cord-253024-b393ea2u	419	1	Isolation	isolation	NN
cord-253024-b393ea2u	419	2	,	,	,
cord-253024-b393ea2u	419	3	characterlzatlon	characterlzatlon	NN
cord-253024-b393ea2u	419	4	and	and	CC
cord-253024-b393ea2u	419	5	neuropathogenlc	neuropathogenlc	NN
cord-253024-b393ea2u	419	6	properties	property	NNS
cord-253024-b393ea2u	419	7	Coronavlrus	Coronavlrus	NNP
cord-253024-b393ea2u	419	8	:	:	:
cord-253024-b393ea2u	419	9	Organization	organization	NN
cord-253024-b393ea2u	419	10	,	,	,
cord-253024-b393ea2u	419	11	replication	replication	NN
cord-253024-b393ea2u	419	12	and	and	CC
cord-253024-b393ea2u	419	13	expression	expression	NN
cord-253024-b393ea2u	419	14	of	of	IN
cord-253024-b393ea2u	419	15	genome	genome	NN
cord-253024-b393ea2u	419	16	Recombination	recombination	NN
cord-253024-b393ea2u	419	17	between	between	IN
cord-253024-b393ea2u	419	18	nonsegmented	nonsegmented	JJ
cord-253024-b393ea2u	419	19	RNA	RNA	NNP
cord-253024-b393ea2u	419	20	genomes	genome	NNS
cord-253024-b393ea2u	419	21	of	of	IN
cord-253024-b393ea2u	419	22	murine	murine	JJ
cord-253024-b393ea2u	419	23	coronavirus	coronavirus	NN
cord-253024-b393ea2u	420	1	A	a	DT
cord-253024-b393ea2u	420	2	genetic	genetic	JJ
cord-253024-b393ea2u	420	3	recomblnatlon	recomblnatlon	NN
cord-253024-b393ea2u	420	4	map	map	NN
cord-253024-b393ea2u	420	5	of	of	IN
cord-253024-b393ea2u	420	6	foot	foot	NN
cord-253024-b393ea2u	420	7	-	-	HYPH
cord-253024-b393ea2u	420	8	and	and	CC
cord-253024-b393ea2u	420	9	-	-	HYPH
cord-253024-b393ea2u	420	10	mouth	mouth	NN
cord-253024-b393ea2u	420	11	disease	disease	NN
cord-253024-b393ea2u	420	12	virus	virus	NN
cord-253024-b393ea2u	420	13	.	.	.
cord-253024-b393ea2u	421	1	I.	I.	NNP
cord-253024-b393ea2u	421	2	Gem	Gem	NNP
cord-253024-b393ea2u	421	3	Viral	Viral	NNP
cord-253024-b393ea2u	421	4	Locallzatlon	Locallzatlon	NNP
cord-253024-b393ea2u	421	5	of	of	IN
cord-253024-b393ea2u	421	6	extensive	extensive	JJ
cord-253024-b393ea2u	421	7	deletions	deletion	NNS
cord-253024-b393ea2u	421	8	in	in	IN
cord-253024-b393ea2u	421	9	the	the	DT
cord-253024-b393ea2u	421	10	structural	structural	JJ
cord-253024-b393ea2u	421	11	genes	gene	NNS
cord-253024-b393ea2u	421	12	of	of	IN
cord-253024-b393ea2u	421	13	two	two	CD
cord-253024-b393ea2u	421	14	neurotroplc	neurotroplc	NNP
cord-253024-b393ea2u	421	15	variants	variant	NNS
cord-253024-b393ea2u	421	16	of	of	IN
cord-253024-b393ea2u	421	17	murine	murine	JJ
cord-253024-b393ea2u	421	18	coronavirus	coronavirus	NN
cord-253024-b393ea2u	421	19	JHM	JHM	NNP
cord-253024-b393ea2u	422	1	The	the	DT
cord-253024-b393ea2u	422	2	complete	complete	JJ
cord-253024-b393ea2u	422	3	sequence	sequence	NN
cord-253024-b393ea2u	422	4	(	(	-LRB-
cord-253024-b393ea2u	422	5	22	22	CD
cord-253024-b393ea2u	422	6	kilobases	kilobase	NNS
cord-253024-b393ea2u	422	7	)	)	-RRB-
cord-253024-b393ea2u	422	8	of	of	IN
cord-253024-b393ea2u	422	9	murine	murine	JJ
cord-253024-b393ea2u	422	10	coronavirus	coronavirus	NN
cord-253024-b393ea2u	422	11	gene	gene	NN
cord-253024-b393ea2u	422	12	1	1	CD
cord-253024-b393ea2u	422	13	encoding	encode	VBG
cord-253024-b393ea2u	422	14	the	the	DT
cord-253024-b393ea2u	422	15	putative	putative	JJ
cord-253024-b393ea2u	422	16	proteases	protease	NNS
cord-253024-b393ea2u	422	17	and	and	CC
cord-253024-b393ea2u	422	18	RNA	RNA	NNP
cord-253024-b393ea2u	422	19	polymerase	polymerase	NN
cord-253024-b393ea2u	422	20	Genetic	genetic	JJ
cord-253024-b393ea2u	422	21	analysis	analysis	NN
cord-253024-b393ea2u	422	22	of	of	IN
cord-253024-b393ea2u	422	23	munne	munne	NNP
cord-253024-b393ea2u	422	24	hepatltls	hepatltls	NNP
cord-253024-b393ea2u	422	25	virus	virus	NNP
cord-253024-b393ea2u	422	26	strain	strain	VBP
cord-253024-b393ea2u	422	27	JHM	JHM	NNP
cord-253024-b393ea2u	422	28	Primary	primary	JJ
cord-253024-b393ea2u	422	29	structure	structure	NN
cord-253024-b393ea2u	422	30	of	of	IN
cord-253024-b393ea2u	422	31	the	the	DT
cord-253024-b393ea2u	422	32	glycoproteln	glycoproteln	NN
cord-253024-b393ea2u	422	33	E2	E2	NNP
cord-253024-b393ea2u	422	34	of	of	IN
cord-253024-b393ea2u	422	35	coronavlrus	coronavlrus	NNP
cord-253024-b393ea2u	422	36	MHV	MHV	NNP
cord-253024-b393ea2u	422	37	-	-	HYPH
cord-253024-b393ea2u	422	38	A59	A59	NNP
cord-253024-b393ea2u	422	39	and	and	CC
cord-253024-b393ea2u	422	40	ldentlfication	ldentlfication	NN
cord-253024-b393ea2u	422	41	of	of	IN
cord-253024-b393ea2u	422	42	the	the	DT
cord-253024-b393ea2u	422	43	trypsln	trypsln	NNP
cord-253024-b393ea2u	422	44	cleavage	cleavage	NN
cord-253024-b393ea2u	422	45	site	site	NN
cord-253024-b393ea2u	422	46	Sequence	sequence	NN
cord-253024-b393ea2u	422	47	of	of	IN
cord-253024-b393ea2u	422	48	mouse	mouse	NN
cord-253024-b393ea2u	422	49	hepatitis	hepatitis	NN
cord-253024-b393ea2u	422	50	virus	virus	NN
cord-253024-b393ea2u	422	51	A59	A59	NNP
cord-253024-b393ea2u	422	52	mRNA2	mrna2	NN
cord-253024-b393ea2u	422	53	:	:	:
cord-253024-b393ea2u	423	1	Indications	indication	NNS
cord-253024-b393ea2u	423	2	for	for	IN
cord-253024-b393ea2u	423	3	RNA	rna	NN
cord-253024-b393ea2u	423	4	-	-	HYPH
cord-253024-b393ea2u	423	5	recombination	recombination	NN
cord-253024-b393ea2u	423	6	between	between	IN
cord-253024-b393ea2u	423	7	coronavlruses	coronavlruse	NNS
cord-253024-b393ea2u	423	8	and	and	CC
cord-253024-b393ea2u	423	9	Influenza	Influenza	NNP
cord-253024-b393ea2u	423	10	C	C	NNP
cord-253024-b393ea2u	423	11	virus	virus	NN
cord-253024-b393ea2u	423	12	RNA	RNA	NNP
cord-253024-b393ea2u	423	13	dependent	dependent	JJ
cord-253024-b393ea2u	423	14	RNA	RNA	NNP
cord-253024-b393ea2u	423	15	polymerase	polymerase	NN
cord-253024-b393ea2u	423	16	activity	activity	NN
cord-253024-b393ea2u	423	17	in	in	IN
cord-253024-b393ea2u	423	18	munne	munne	NN
cord-253024-b393ea2u	423	19	coronavirus	coronavirus	NN
cord-253024-b393ea2u	423	20	infected	infect	VBN
cord-253024-b393ea2u	423	21	cells	cell	NNS
cord-253024-b393ea2u	423	22	Leader	leader	NN
cord-253024-b393ea2u	423	23	sequences	sequence	NNS
cord-253024-b393ea2u	423	24	of	of	IN
cord-253024-b393ea2u	423	25	murine	murine	JJ
cord-253024-b393ea2u	423	26	coronavlrus	coronavlrus	NNP
cord-253024-b393ea2u	423	27	mRNAs	mrnas	WDT
cord-253024-b393ea2u	423	28	can	can	MD
cord-253024-b393ea2u	423	29	be	be	VB
cord-253024-b393ea2u	423	30	freely	freely	RB
cord-253024-b393ea2u	423	31	reassorted	reassorte	VBN
cord-253024-b393ea2u	423	32	.	.	.
cord-253024-b393ea2u	424	1	Evidence	evidence	NN
cord-253024-b393ea2u	424	2	for	for	IN
cord-253024-b393ea2u	424	3	the	the	DT
cord-253024-b393ea2u	424	4	role	role	NN
cord-253024-b393ea2u	424	5	of	of	IN
cord-253024-b393ea2u	424	6	free	free	JJ
cord-253024-b393ea2u	424	7	leader	leader	NN
cord-253024-b393ea2u	424	8	RNA	RNA	NNP
cord-253024-b393ea2u	424	9	In	in	IN
cord-253024-b393ea2u	424	10	transcription	transcription	NN
cord-253024-b393ea2u	424	11	High	high	JJ
cord-253024-b393ea2u	424	12	frequency	frequency	NN
cord-253024-b393ea2u	424	13	RNA	RNA	NNP
cord-253024-b393ea2u	424	14	recombination	recombination	NN
cord-253024-b393ea2u	424	15	of	of	IN
cord-253024-b393ea2u	424	16	munne	munne	NNP
cord-253024-b393ea2u	424	17	coronaviruses	coronaviruses	NNP
cord-253024-b393ea2u	424	18	RNA	RNA	NNP
cord-253024-b393ea2u	424	19	recombination	recombination	NN
cord-253024-b393ea2u	424	20	of	of	IN
cord-253024-b393ea2u	424	21	coronaviruses	coronaviruse	NNS
cord-253024-b393ea2u	424	22	;	;	:
cord-253024-b393ea2u	424	23	localization	localization	NN
cord-253024-b393ea2u	424	24	of	of	IN
cord-253024-b393ea2u	424	25	neutralizing	neutralize	VBG
cord-253024-b393ea2u	424	26	epitopes	epitope	NNS
cord-253024-b393ea2u	424	27	and	and	CC
cord-253024-b393ea2u	424	28	neuropathogenlc	neuropathogenlc	RB
cord-253024-b393ea2u	424	29	determinants	determinant	NNS
cord-253024-b393ea2u	424	30	on	on	IN
cord-253024-b393ea2u	424	31	the	the	DT
cord-253024-b393ea2u	424	32	carboxy	carboxy	JJ
cord-253024-b393ea2u	424	33	terminus	terminus	NN
cord-253024-b393ea2u	424	34	of	of	IN
cord-253024-b393ea2u	424	35	peplomers	peplomers	NNP
cord-253024-b393ea2u	424	36	Prlmary	Prlmary	NNP
cord-253024-b393ea2u	424	37	structure	structure	NN
cord-253024-b393ea2u	424	38	and	and	CC
cord-253024-b393ea2u	424	39	translation	translation	NN
cord-253024-b393ea2u	424	40	of	of	IN
cord-253024-b393ea2u	424	41	a	a	DT
cord-253024-b393ea2u	424	42	defective	defective	JJ
cord-253024-b393ea2u	424	43	Interfering	Interfering	NNP
cord-253024-b393ea2u	424	44	RNA	RNA	NNP
cord-253024-b393ea2u	424	45	of	of	IN
cord-253024-b393ea2u	424	46	murlne	murlne	NNP
cord-253024-b393ea2u	424	47	coronavtrus	coronavtrus	NNP
cord-253024-b393ea2u	424	48	High	High	NNP
cord-253024-b393ea2u	424	49	frequency	frequency	NN
cord-253024-b393ea2u	424	50	leader	leader	NN
cord-253024-b393ea2u	424	51	sequence	sequence	NN
cord-253024-b393ea2u	424	52	switching	switch	VBG
cord-253024-b393ea2u	424	53	during	during	IN
cord-253024-b393ea2u	424	54	coronavirus	coronavirus	NN
cord-253024-b393ea2u	424	55	defective	defective	JJ
cord-253024-b393ea2u	424	56	RNA	RNA	NNP
cord-253024-b393ea2u	424	57	replication	replication	NN
cord-253024-b393ea2u	424	58	.	.	.
cord-253024-b393ea2u	425	1	I	-PRON-	PRP
cord-253024-b393ea2u	426	1	Viroi	Viroi	NNP
cord-253024-b393ea2u	426	2	A	a	DT
cord-253024-b393ea2u	426	3	system	system	NN
cord-253024-b393ea2u	426	4	for	for	IN
cord-253024-b393ea2u	426	5	study	study	NN
cord-253024-b393ea2u	426	6	of	of	IN
cord-253024-b393ea2u	426	7	coronavlrus	coronavlrus	NNP
cord-253024-b393ea2u	426	8	mRNA	mRNA	NNP
cord-253024-b393ea2u	426	9	synthesis	synthesis	NNP
cord-253024-b393ea2u	427	1	:	:	:
cord-253024-b393ea2u	427	2	a	a	DT
cord-253024-b393ea2u	427	3	regulated	regulate	VBN
cord-253024-b393ea2u	427	4	,	,	,
cord-253024-b393ea2u	427	5	expressed	express	VBD
cord-253024-b393ea2u	427	6	subgenomlc	subgenomlc	NNP
cord-253024-b393ea2u	427	7	defective	defective	JJ
cord-253024-b393ea2u	427	8	interfering	interfere	VBG
cord-253024-b393ea2u	427	9	RNA	RNA	NNP
cord-253024-b393ea2u	427	10	results	result	NNS
cord-253024-b393ea2u	427	11	from	from	IN
cord-253024-b393ea2u	427	12	lntergenlc	lntergenlc	NNP
cord-253024-b393ea2u	427	13	Insertion	Insertion	NNP
cord-253024-b393ea2u	427	14	Temperature	Temperature	NNP
cord-253024-b393ea2u	427	15	sensitive	sensitive	JJ
cord-253024-b393ea2u	427	16	mutants	mutant	NNS
cord-253024-b393ea2u	427	17	of	of	IN
cord-253024-b393ea2u	427	18	mouse	mouse	NN
cord-253024-b393ea2u	427	19	hepatitis	hepatitis	NNP
cord-253024-b393ea2u	427	20	virus	virus	NN
cord-253024-b393ea2u	427	21	type	type	NN
cord-253024-b393ea2u	427	22	3(MHV-3	3(mhv-3	CD
cord-253024-b393ea2u	427	23	)	)	-RRB-
cord-253024-b393ea2u	428	1	:	:	:
cord-253024-b393ea2u	428	2	Isolation	isolation	NN
cord-253024-b393ea2u	428	3	,	,	,
cord-253024-b393ea2u	428	4	biochemical	biochemical	JJ
cord-253024-b393ea2u	428	5	and	and	CC
cord-253024-b393ea2u	428	6	genetic	genetic	JJ
cord-253024-b393ea2u	428	7	characterization	characterization	NN
cord-253024-b393ea2u	428	8	RNAs	rna	NNS
cord-253024-b393ea2u	428	9	from	from	IN
cord-253024-b393ea2u	428	10	two	two	CD
cord-253024-b393ea2u	428	11	Independently	independently	RB
cord-253024-b393ea2u	428	12	Isolated	isolate	VBN
cord-253024-b393ea2u	428	13	defective	defective	JJ
cord-253024-b393ea2u	428	14	interfering	interfere	VBG
cord-253024-b393ea2u	428	15	parttcles	parttcle	NNS
cord-253024-b393ea2u	428	16	of	of	IN
cord-253024-b393ea2u	428	17	Sinbdis	Sinbdis	NNP
cord-253024-b393ea2u	428	18	virus	virus	NN
cord-253024-b393ea2u	428	19	contain	contain	VBP
cord-253024-b393ea2u	428	20	a	a	DT
cord-253024-b393ea2u	428	21	cellular	cellular	JJ
cord-253024-b393ea2u	428	22	RNA	rna	NN
cord-253024-b393ea2u	428	23	sequence	sequence	NN
cord-253024-b393ea2u	428	24	at	at	IN
cord-253024-b393ea2u	428	25	their	-PRON-	PRP$
cord-253024-b393ea2u	428	26	5'ends	5'ends	CD
cord-253024-b393ea2u	428	27	Molecular	molecular	JJ
cord-253024-b393ea2u	428	28	cloning	cloning	NN
cord-253024-b393ea2u	428	29	of	of	IN
cord-253024-b393ea2u	428	30	the	the	DT
cord-253024-b393ea2u	428	31	gene	gene	NN
cord-253024-b393ea2u	428	32	encodlng	encodlng	NN
cord-253024-b393ea2u	428	33	the	the	DT
cord-253024-b393ea2u	428	34	putative	putative	JJ
cord-253024-b393ea2u	428	35	polymerase	polymerase	NN
cord-253024-b393ea2u	428	36	of	of	IN
cord-253024-b393ea2u	428	37	mouse	mouse	NN
cord-253024-b393ea2u	428	38	hepatltls	hepatltls	NNP
cord-253024-b393ea2u	428	39	coronavirus	coronavirus	NN
cord-253024-b393ea2u	428	40	,	,	,
cord-253024-b393ea2u	428	41	strain	strain	VB
cord-253024-b393ea2u	428	42	A59	A59	NNP
cord-253024-b393ea2u	428	43	Sequence	Sequence	NNP
cord-253024-b393ea2u	428	44	analysis	analysis	NN
cord-253024-b393ea2u	428	45	reveals	reveal	VBZ
cord-253024-b393ea2u	428	46	extensive	extensive	JJ
cord-253024-b393ea2u	428	47	polymorphism	polymorphism	NN
cord-253024-b393ea2u	428	48	and	and	CC
cord-253024-b393ea2u	428	49	evidence	evidence	NN
cord-253024-b393ea2u	428	50	of	of	IN
cord-253024-b393ea2u	428	51	deletions	deletion	NNS
cord-253024-b393ea2u	428	52	wlthin	wlthin	NNP
cord-253024-b393ea2u	428	53	the	the	DT
cord-253024-b393ea2u	428	54	E2	E2	NNP
cord-253024-b393ea2u	428	55	glycoprotetn	glycoprotetn	NNS
cord-253024-b393ea2u	428	56	gene	gene	NN
cord-253024-b393ea2u	428	57	of	of	IN
cord-253024-b393ea2u	428	58	several	several	JJ
cord-253024-b393ea2u	428	59	strains	strain	NNS
cord-253024-b393ea2u	428	60	of	of	IN
cord-253024-b393ea2u	428	61	munne	munne	NNP
cord-253024-b393ea2u	428	62	hepatitis	hepatitis	NNP
cord-253024-b393ea2u	428	63	virus	virus	NN
cord-253024-b393ea2u	428	64	Mroiogy	Mroiogy	NNP
cord-253024-b393ea2u	428	65	Porcine	porcine	JJ
cord-253024-b393ea2u	428	66	respiratory	respiratory	JJ
cord-253024-b393ea2u	428	67	coronavirus	coronavirus	NN
cord-253024-b393ea2u	428	68	differs	differ	VBZ
cord-253024-b393ea2u	428	69	from	from	IN
cord-253024-b393ea2u	428	70	transmissible	transmissible	JJ
cord-253024-b393ea2u	428	71	gastroenteritis	gastroenteritis	NN
cord-253024-b393ea2u	428	72	virus	virus	NN
cord-253024-b393ea2u	428	73	by	by	IN
cord-253024-b393ea2u	428	74	a	a	DT
cord-253024-b393ea2u	428	75	few	few	JJ
cord-253024-b393ea2u	428	76	genomic	genomic	JJ
cord-253024-b393ea2u	428	77	deletions	deletion	NNS
cord-253024-b393ea2u	428	78	Analysis	Analysis	NNP
cord-253024-b393ea2u	428	79	of	of	IN
cord-253024-b393ea2u	428	80	murine	murine	JJ
cord-253024-b393ea2u	428	81	coronavirus	coronavirus	NN
cord-253024-b393ea2u	428	82	surface	surface	NN
cord-253024-b393ea2u	428	83	glycoproteins	glycoprotein	NNS
cord-253024-b393ea2u	428	84	functions	function	NNS
cord-253024-b393ea2u	428	85	by	by	IN
cord-253024-b393ea2u	428	86	using	use	VBG
cord-253024-b393ea2u	428	87	monoclonal	monoclonal	JJ
cord-253024-b393ea2u	428	88	antibodies	antibody	NNS
cord-253024-b393ea2u	428	89	.	.	.
cord-253024-b393ea2u	429	1	1	1	LS
cord-253024-b393ea2u	429	2	.	.	.
cord-253024-b393ea2u	430	1	Viral	Viral	NNP
cord-253024-b393ea2u	430	2	Poliovirus	Poliovirus	NNP
cord-253024-b393ea2u	430	3	genetics	genetics	NN
cord-253024-b393ea2u	430	4	Coronavirus	Coronavirus	NNP
cord-253024-b393ea2u	430	5	transcription	transcription	NN
cord-253024-b393ea2u	430	6	:	:	:
cord-253024-b393ea2u	431	1	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	431	2	mouse	mouse	NNP
cord-253024-b393ea2u	431	3	hepatitis	hepatitis	NNP
cord-253024-b393ea2u	431	4	virus	virus	NN
cord-253024-b393ea2u	431	5	replicative	replicative	JJ
cord-253024-b393ea2u	431	6	intermediates	intermediate	NNS
cord-253024-b393ea2u	431	7	function	function	VBP
cord-253024-b393ea2u	431	8	in	in	IN
cord-253024-b393ea2u	431	9	RNA	RNA	NNP
cord-253024-b393ea2u	431	10	synthesis	synthesis	NN
cord-253024-b393ea2u	431	11	Genetics	genetic	NNS
cord-253024-b393ea2u	431	12	of	of	IN
cord-253024-b393ea2u	431	13	murine	murine	JJ
cord-253024-b393ea2u	431	14	coronavirus	coronavirus	NN
cord-253024-b393ea2u	431	15	transcription	transcription	NN
cord-253024-b393ea2u	431	16	:	:	:
cord-253024-b393ea2u	431	17	Identification	identification	NN
cord-253024-b393ea2u	431	18	of	of	IN
cord-253024-b393ea2u	431	19	a	a	DT
cord-253024-b393ea2u	431	20	cistron	cistron	NN
cord-253024-b393ea2u	431	21	required	require	VBN
cord-253024-b393ea2u	431	22	for	for	IN
cord-253024-b393ea2u	431	23	MHV	MHV	NNP
cord-253024-b393ea2u	431	24	negative	negative	JJ
cord-253024-b393ea2u	431	25	strand	strand	NN
cord-253024-b393ea2u	431	26	synthesis	synthesis	NN
cord-253024-b393ea2u	431	27	Nucleotide	nucleotide	JJ
cord-253024-b393ea2u	431	28	sequence	sequence	NN
cord-253024-b393ea2u	431	29	of	of	IN
cord-253024-b393ea2u	431	30	the	the	DT
cord-253024-b393ea2u	431	31	gene	gene	NN
cord-253024-b393ea2u	431	32	encoding	encode	VBG
cord-253024-b393ea2u	431	33	the	the	DT
cord-253024-b393ea2u	431	34	surface	surface	NN
cord-253024-b393ea2u	431	35	projection	projection	NN
cord-253024-b393ea2u	431	36	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	431	37	of	of	IN
cord-253024-b393ea2u	432	1	coronavirus	coronavirus	NN
cord-253024-b393ea2u	432	2	MHV	MHV	NNP
cord-253024-b393ea2u	432	3	-	-	HYPH
cord-253024-b393ea2u	432	4	JHM	JHM	NNP
cord-253024-b393ea2u	432	5	Murine	Murine	NNP
cord-253024-b393ea2u	433	1	coronavirus	coronavirus	NNP
cord-253024-b393ea2u	433	2	nonstructural	nonstructural	JJ
cord-253024-b393ea2u	433	3	protein	protein	NN
cord-253024-b393ea2u	433	4	ns2	ns2	NNP
cord-253024-b393ea2u	433	5	is	be	VBZ
cord-253024-b393ea2u	433	6	not	not	RB
cord-253024-b393ea2u	433	7	essential	essential	JJ
cord-253024-b393ea2u	433	8	forvirus	forvirus	JJ
cord-253024-b393ea2u	433	9	replication	replication	NN
cord-253024-b393ea2u	433	10	in	in	IN
cord-253024-b393ea2u	433	11	transformed	transform	VBN
cord-253024-b393ea2u	433	12	cells	cell	NNS
cord-253024-b393ea2u	434	1	Coronavirus	Coronavirus	NNP
cord-253024-b393ea2u	434	2	subgenomic	subgenomic	JJ
cord-253024-b393ea2u	434	3	minus	minus	JJ
cord-253024-b393ea2u	434	4	-	-	HYPH
cord-253024-b393ea2u	434	5	strand	strand	NN
cord-253024-b393ea2u	434	6	RNAs	rna	NNS
cord-253024-b393ea2u	434	7	and	and	CC
cord-253024-b393ea2u	434	8	the	the	DT
cord-253024-b393ea2u	434	9	potential	potential	NN
cord-253024-b393ea2u	434	10	for	for	IN
cord-253024-b393ea2u	434	11	mRNA	mRNA	NNP
cord-253024-b393ea2u	434	12	replicons	replicons	NNPS
cord-253024-b393ea2u	434	13	Minus	Minus	NNP
cord-253024-b393ea2u	434	14	-	-	HYPH
cord-253024-b393ea2u	434	15	strand	strand	NN
cord-253024-b393ea2u	434	16	copies	copy	NNS
cord-253024-b393ea2u	434	17	of	of	IN
cord-253024-b393ea2u	434	18	replicating	replicate	VBG
cord-253024-b393ea2u	434	19	coronavirus	coronavirus	NN
cord-253024-b393ea2u	435	1	mRNAs	mrnas	LS
cord-253024-b393ea2u	435	2	contain	contain	VBP
cord-253024-b393ea2u	435	3	antileaders	antileader	NNS
cord-253024-b393ea2u	435	4	Identification	Identification	NNP
cord-253024-b393ea2u	435	5	of	of	IN
cord-253024-b393ea2u	435	6	a	a	DT
cord-253024-b393ea2u	435	7	new	new	JJ
cord-253024-b393ea2u	435	8	transcriptional	transcriptional	JJ
cord-253024-b393ea2u	435	9	initiation	initiation	NN
cord-253024-b393ea2u	435	10	site	site	NN
cord-253024-b393ea2u	435	11	and	and	CC
cord-253024-b393ea2u	435	12	the	the	DT
cord-253024-b393ea2u	435	13	corresponding	corresponding	JJ
cord-253024-b393ea2u	435	14	functional	functional	JJ
cord-253024-b393ea2u	435	15	gene	gene	NN
cord-253024-b393ea2u	435	16	2b	2b	CD
cord-253024-b393ea2u	435	17	in	in	IN
cord-253024-b393ea2u	435	18	the	the	DT
cord-253024-b393ea2u	435	19	murine	murine	JJ
cord-253024-b393ea2u	435	20	coronavirus	coronavirus	NN
cord-253024-b393ea2u	435	21	RNA	RNA	NNP
cord-253024-b393ea2u	435	22	genome	genome	NN
cord-253024-b393ea2u	435	23	Coronavirus	Coronavirus	NNP
cord-253024-b393ea2u	435	24	JHM	JHM	NNP
cord-253024-b393ea2u	435	25	:	:	:
cord-253024-b393ea2u	436	1	Coding	coding	NN
cord-253024-b393ea2u	436	2	assignments	assignment	NNS
cord-253024-b393ea2u	436	3	of	of	IN
cord-253024-b393ea2u	436	4	subgenomic	subgenomic	NNP
cord-253024-b393ea2u	437	1	mRNAs	mRNAs	.
cord-253024-b393ea2u	438	1	Comparison	comparison	NN
cord-253024-b393ea2u	438	2	of	of	IN
cord-253024-b393ea2u	438	3	the	the	DT
cord-253024-b393ea2u	438	4	genome	genome	NN
cord-253024-b393ea2u	438	5	organization	organization	NN
cord-253024-b393ea2u	438	6	of	of	IN
cord-253024-b393ea2u	438	7	Toro	Toro	NNP
cord-253024-b393ea2u	438	8	-	-	HYPH
cord-253024-b393ea2u	438	9	and	and	CC
cord-253024-b393ea2u	438	10	Coronaviruses	Coronaviruses	NNP
cord-253024-b393ea2u	438	11	:	:	:
cord-253024-b393ea2u	439	1	Evidence	evidence	NN
cord-253024-b393ea2u	439	2	for	for	IN
cord-253024-b393ea2u	439	3	two	two	CD
cord-253024-b393ea2u	439	4	nonhomologous	nonhomologous	NNP
cord-253024-b393ea2u	439	5	RNA	RNA	NNP
cord-253024-b393ea2u	439	6	recombination	recombination	NN
cord-253024-b393ea2u	439	7	events	event	NNS
cord-253024-b393ea2u	439	8	during	during	IN
cord-253024-b393ea2u	439	9	Berne	Berne	NNP
cord-253024-b393ea2u	439	10	virus	virus	NN
cord-253024-b393ea2u	439	11	Evolution	evolution	NN
cord-253024-b393ea2u	439	12	Sequence	sequence	NN
cord-253024-b393ea2u	439	13	and	and	CC
cord-253024-b393ea2u	439	14	translation	translation	NN
cord-253024-b393ea2u	439	15	of	of	IN
cord-253024-b393ea2u	439	16	the	the	DT
cord-253024-b393ea2u	439	17	murine	murine	JJ
cord-253024-b393ea2u	439	18	coronavirus	coronavirus	NN
cord-253024-b393ea2u	439	19	5'.end	5'.end	CD
cord-253024-b393ea2u	439	20	genomic	genomic	JJ
cord-253024-b393ea2u	439	21	RNA	RNA	NNP
cord-253024-b393ea2u	439	22	reveals	reveal	VBZ
cord-253024-b393ea2u	439	23	the	the	DT
cord-253024-b393ea2u	439	24	N	n	CD
cord-253024-b393ea2u	439	25	-	-	HYPH
cord-253024-b393ea2u	439	26	terminal	terminal	JJ
cord-253024-b393ea2u	439	27	structure	structure	NN
cord-253024-b393ea2u	439	28	of	of	IN
cord-253024-b393ea2u	439	29	the	the	DT
cord-253024-b393ea2u	439	30	putative	putative	JJ
cord-253024-b393ea2u	439	31	polymerase	polymerase	NN
cord-253024-b393ea2u	439	32	Specific	specific	JJ
cord-253024-b393ea2u	439	33	interaction	interaction	NN
cord-253024-b393ea2u	439	34	between	between	IN
cord-253024-b393ea2u	439	35	the	the	DT
cord-253024-b393ea2u	439	36	coronavirus	coronavirus	NN
cord-253024-b393ea2u	439	37	leader	leader	NN
cord-253024-b393ea2u	439	38	RNA	RNA	NNP
cord-253024-b393ea2u	439	39	and	and	CC
cord-253024-b393ea2u	439	40	nucleocapsid	nucleocapsid	NN
cord-253024-b393ea2u	439	41	protein	protein	NN
cord-253024-b393ea2u	439	42	Phosphoproteins	Phosphoproteins	NNP
cord-253024-b393ea2u	439	43	of	of	IN
cord-253024-b393ea2u	439	44	murine	murine	JJ
cord-253024-b393ea2u	439	45	hepatitis	hepatitis	NN
cord-253024-b393ea2u	439	46	virus	virus	NN
cord-253024-b393ea2u	439	47	Isolation	isolation	NN
cord-253024-b393ea2u	439	48	of	of	IN
cord-253024-b393ea2u	439	49	coronavirus	coronavirus	NN
cord-253024-b393ea2u	439	50	envelope	envelope	NN
cord-253024-b393ea2u	439	51	glycoproteins	glycoprotein	NNS
cord-253024-b393ea2u	439	52	and	and	CC
cord-253024-b393ea2u	439	53	interaction	interaction	NN
cord-253024-b393ea2u	439	54	with	with	IN
cord-253024-b393ea2u	439	55	the	the	DT
cord-253024-b393ea2u	439	56	viral	viral	JJ
cord-253024-b393ea2u	439	57	nucleocapsid	nucleocapsid	NN
cord-253024-b393ea2u	439	58	.	.	.
cord-253024-b393ea2u	440	1	1	1	LS
cord-253024-b393ea2u	440	2	.	.	.
cord-253024-b393ea2u	441	1	Viral	Viral	NNP
cord-253024-b393ea2u	441	2	The	the	DT
cord-253024-b393ea2u	441	3	novel	novel	JJ
cord-253024-b393ea2u	441	4	glycoproteins	glycoprotein	NNS
cord-253024-b393ea2u	441	5	of	of	IN
cord-253024-b393ea2u	441	6	coronaviruses	coronaviruse	NNS
cord-253024-b393ea2u	441	7	Proteolytic	proteolytic	JJ
cord-253024-b393ea2u	441	8	cleavage	cleavage	NN
cord-253024-b393ea2u	441	9	of	of	IN
cord-253024-b393ea2u	441	10	the	the	DT
cord-253024-b393ea2u	441	11	E2	E2	NNP
cord-253024-b393ea2u	441	12	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	441	13	of	of	IN
cord-253024-b393ea2u	441	14	murine	murine	JJ
cord-253024-b393ea2u	441	15	coronaviruses	coronaviruse	NNS
cord-253024-b393ea2u	441	16	:	:	:
cord-253024-b393ea2u	442	1	Activation	activation	NN
cord-253024-b393ea2u	442	2	of	of	IN
cord-253024-b393ea2u	442	3	cell	cell	NN
cord-253024-b393ea2u	442	4	fusing	fusing	NN
cord-253024-b393ea2u	442	5	activity	activity	NN
cord-253024-b393ea2u	442	6	of	of	IN
cord-253024-b393ea2u	442	7	virions	virion	NNS
cord-253024-b393ea2u	442	8	by	by	IN
cord-253024-b393ea2u	442	9	trypsin	trypsin	NN
cord-253024-b393ea2u	442	10	and	and	CC
cord-253024-b393ea2u	442	11	separation	separation	NN
cord-253024-b393ea2u	442	12	of	of	IN
cord-253024-b393ea2u	442	13	two	two	CD
cord-253024-b393ea2u	442	14	different	different	JJ
cord-253024-b393ea2u	442	15	90	90	CD
cord-253024-b393ea2u	442	16	K	k	NN
cord-253024-b393ea2u	442	17	cleavage	cleavage	NN
cord-253024-b393ea2u	442	18	fragments	fragment	NNS
cord-253024-b393ea2u	442	19	Characterization	characterization	NN
cord-253024-b393ea2u	442	20	of	of	IN
cord-253024-b393ea2u	442	21	JHMV	JHMV	NNP
cord-253024-b393ea2u	442	22	variants	variant	NNS
cord-253024-b393ea2u	442	23	isolated	isolate	VBN
cord-253024-b393ea2u	442	24	from	from	IN
cord-253024-b393ea2u	442	25	rat	rat	NN
cord-253024-b393ea2u	442	26	brain	brain	NN
cord-253024-b393ea2u	442	27	and	and	CC
cord-253024-b393ea2u	442	28	cultured	cultured	JJ
cord-253024-b393ea2u	442	29	neural	neural	JJ
cord-253024-b393ea2u	442	30	cells	cell	NNS
cord-253024-b393ea2u	442	31	after	after	IN
cord-253024-b393ea2u	442	32	wild	wild	JJ
cord-253024-b393ea2u	442	33	type	type	NN
cord-253024-b393ea2u	442	34	JHMV	JHMV	NNP
cord-253024-b393ea2u	442	35	infection	infection	NN
cord-253024-b393ea2u	442	36	Studies	Studies	NNPS
cord-253024-b393ea2u	442	37	on	on	IN
cord-253024-b393ea2u	442	38	the	the	DT
cord-253024-b393ea2u	442	39	recombination	recombination	NN
cord-253024-b393ea2u	442	40	between	between	IN
cord-253024-b393ea2u	442	41	RNA	RNA	NNP
cord-253024-b393ea2u	442	42	genomes	genome	NNS
cord-253024-b393ea2u	442	43	of	of	IN
cord-253024-b393ea2u	442	44	poliovirus	poliovirus	NN
cord-253024-b393ea2u	442	45	:	:	:
cord-253024-b393ea2u	442	46	The	the	DT
cord-253024-b393ea2u	442	47	primary	primary	JJ
cord-253024-b393ea2u	442	48	structure	structure	NN
cord-253024-b393ea2u	442	49	and	and	CC
cord-253024-b393ea2u	442	50	nonrandom	nonrandom	JJ
cord-253024-b393ea2u	442	51	distribution	distribution	NN
cord-253024-b393ea2u	442	52	of	of	IN
cord-253024-b393ea2u	442	53	crossover	crossover	NN
cord-253024-b393ea2u	442	54	regions	region	NNS
cord-253024-b393ea2u	442	55	in	in	IN
cord-253024-b393ea2u	442	56	the	the	DT
cord-253024-b393ea2u	442	57	genomes	genome	NNS
cord-253024-b393ea2u	442	58	of	of	IN
cord-253024-b393ea2u	442	59	intertypic	intertypic	JJ
cord-253024-b393ea2u	442	60	poliovirus	poliovirus	NN
cord-253024-b393ea2u	442	61	recombinants	recombinant	NNS
cord-253024-b393ea2u	442	62	Monoclonal	monoclonal	JJ
cord-253024-b393ea2u	442	63	antibodies	antibody	NNS
cord-253024-b393ea2u	442	64	to	to	IN
cord-253024-b393ea2u	442	65	the	the	DT
cord-253024-b393ea2u	442	66	peplomer	peplomer	JJ
cord-253024-b393ea2u	442	67	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	442	68	of	of	IN
cord-253024-b393ea2u	442	69	coronavirus	coronavirus	NN
cord-253024-b393ea2u	442	70	mouse	mouse	NN
cord-253024-b393ea2u	442	71	hepatitis	hepatitis	NN
cord-253024-b393ea2u	442	72	virus	virus	NN
cord-253024-b393ea2u	442	73	identify	identify	VB
cord-253024-b393ea2u	442	74	two	two	CD
cord-253024-b393ea2u	442	75	subunits	subunit	NNS
cord-253024-b393ea2u	442	76	and	and	CC
cord-253024-b393ea2u	442	77	detect	detect	VB
cord-253024-b393ea2u	442	78	a	a	DT
cord-253024-b393ea2u	442	79	conformational	conformational	JJ
cord-253024-b393ea2u	442	80	change	change	NN
cord-253024-b393ea2u	442	81	in	in	IN
cord-253024-b393ea2u	442	82	the	the	DT
cord-253024-b393ea2u	442	83	subunit	subunit	NN
cord-253024-b393ea2u	442	84	released	release	VBN
cord-253024-b393ea2u	442	85	under	under	IN
cord-253024-b393ea2u	442	86	mild	mild	JJ
cord-253024-b393ea2u	442	87	alkaline	alkaline	JJ
cord-253024-b393ea2u	442	88	conditions	condition	NNS
cord-253024-b393ea2u	442	89	Recombination	Recombination	NNP
cord-253024-b393ea2u	442	90	between	between	IN
cord-253024-b393ea2u	442	91	sindbis	sindbis	NNP
cord-253024-b393ea2u	442	92	virus	virus	NN
cord-253024-b393ea2u	442	93	RNAs	RNAs	NNP
cord-253024-b393ea2u	442	94	Biosynthesis	Biosynthesis	NNP
cord-253024-b393ea2u	442	95	,	,	,
cord-253024-b393ea2u	442	96	structure	structure	NN
cord-253024-b393ea2u	442	97	,	,	,
cord-253024-b393ea2u	442	98	and	and	CC
cord-253024-b393ea2u	442	99	biological	biological	JJ
cord-253024-b393ea2u	442	100	activities	activity	NNS
cord-253024-b393ea2u	442	101	of	of	IN
cord-253024-b393ea2u	442	102	envelope	envelope	NN
cord-253024-b393ea2u	442	103	protein	protein	NN
cord-253024-b393ea2u	442	104	gp65	gp65	NNP
cord-253024-b393ea2u	442	105	of	of	IN
cord-253024-b393ea2u	442	106	murine	murine	JJ
cord-253024-b393ea2u	442	107	coronavirus	coronavirus	NN
cord-253024-b393ea2u	442	108	Mouse	Mouse	NNP
cord-253024-b393ea2u	442	109	hepatitis	hepatitis	NN
cord-253024-b393ea2u	442	110	virus	virus	NN
cord-253024-b393ea2u	442	111	S	S	NNP
cord-253024-b393ea2u	442	112	RNA	RNA	NNP
cord-253024-b393ea2u	442	113	sequence	sequence	NN
cord-253024-b393ea2u	442	114	revealed	reveal	VBD
cord-253024-b393ea2u	442	115	that	that	IN
cord-253024-b393ea2u	442	116	nonstructural	nonstructural	JJ
cord-253024-b393ea2u	442	117	proteins	protein	NNS
cord-253024-b393ea2u	442	118	ns4	ns4	JJ
cord-253024-b393ea2u	442	119	and	and	CC
cord-253024-b393ea2u	442	120	ns5a	ns5a	NNP
cord-253024-b393ea2u	442	121	are	be	VBP
cord-253024-b393ea2u	442	122	not	not	RB
cord-253024-b393ea2u	442	123	essential	essential	JJ
cord-253024-b393ea2u	442	124	for	for	IN
cord-253024-b393ea2u	442	125	murine	murine	JJ
cord-253024-b393ea2u	442	126	coronavirus	coronavirus	NN
cord-253024-b393ea2u	442	127	replication	replication	NN
cord-253024-b393ea2u	442	128	.	.	.
cord-253024-b393ea2u	443	1	1	1	LS
cord-253024-b393ea2u	443	2	.	.	.
cord-253024-b393ea2u	444	1	Viral	Viral	NNP
cord-253024-b393ea2u	444	2	The	the	DT
cord-253024-b393ea2u	444	3	S2	S2	NNP
cord-253024-b393ea2u	444	4	subunit	subunit	NN
cord-253024-b393ea2u	444	5	of	of	IN
cord-253024-b393ea2u	444	6	the	the	DT
cord-253024-b393ea2u	444	7	spike	spike	NN
cord-253024-b393ea2u	444	8	glycoprotein	glycoprotein	NN
cord-253024-b393ea2u	444	9	of	of	IN
cord-253024-b393ea2u	444	10	bovine	bovine	JJ
cord-253024-b393ea2u	444	11	coronavirus	coronavirus	NN
cord-253024-b393ea2u	444	12	mediates	mediate	VBZ
cord-253024-b393ea2u	444	13	membrane	membrane	NN
cord-253024-b393ea2u	444	14	fusion	fusion	NN
cord-253024-b393ea2u	444	15	in	in	IN
cord-253024-b393ea2u	444	16	insect	insect	NN
cord-253024-b393ea2u	444	17	cells	cell	NNS
cord-253024-b393ea2u	445	1	We	-PRON-	PRP
cord-253024-b393ea2u	445	2	thank	thank	VBP
cord-253024-b393ea2u	445	3	Sheila	Sheila	NNP
cord-253024-b393ea2u	445	4	Peel	Peel	NNP
cord-253024-b393ea2u	445	5	,	,	,
cord-253024-b393ea2u	445	6	Lorraine	Lorraine	NNP
cord-253024-b393ea2u	445	7	Alexander	Alexander	NNP
cord-253024-b393ea2u	445	8	and	and	CC
cord-253024-b393ea2u	445	9	Mary	Mary	NNP
cord-253024-b393ea2u	445	10	Schaad	Schaad	NNP
cord-253024-b393ea2u	445	11	for	for	IN
cord-253024-b393ea2u	445	12	helpful	helpful	JJ
cord-253024-b393ea2u	445	13	comments	comment	NNS
cord-253024-b393ea2u	445	14	and	and	CC
cord-253024-b393ea2u	445	15	criticisms	criticism	NNS
cord-253024-b393ea2u	445	16	.	.	.
cord-253024-b393ea2u	446	1	This	this	DT
cord-253024-b393ea2u	446	2	work	work	NN
cord-253024-b393ea2u	446	3	was	be	VBD
cord-253024-b393ea2u	446	4	supported	support	VBN
cord-253024-b393ea2u	446	5	by	by	IN
cord-253024-b393ea2u	446	6	American	American	NNP
cord-253024-b393ea2u	446	7	Heart	Heart	NNP
cord-253024-b393ea2u	446	8	Association	Association	NNP
cord-253024-b393ea2u	446	9	Grant	Grant	NNP
cord-253024-b393ea2u	446	10	(	(	-LRB-
cord-253024-b393ea2u	446	11	AHA	AHA	NNP
cord-253024-b393ea2u	446	12	87-l	87-l	CD
cord-253024-b393ea2u	446	13	135	135	CD
cord-253024-b393ea2u	446	14	,	,	,
cord-253024-b393ea2u	446	15	AHA	AHA	NNP
cord-253024-b393ea2u	446	16	90	90	CD
cord-253024-b393ea2u	446	17	-	-	HYPH
cord-253024-b393ea2u	446	18	4635	4635	CD
cord-253024-b393ea2u	446	19	)	)	-RRB-
cord-253024-b393ea2u	446	20	and	and	CC
cord-253024-b393ea2u	446	21	a	a	DT
cord-253024-b393ea2u	446	22	grant	grant	NN
cord-253024-b393ea2u	446	23	from	from	IN
cord-253024-b393ea2u	446	24	the	the	DT
cord-253024-b393ea2u	446	25	National	National	NNP
cord-253024-b393ea2u	446	26	Institutes	Institutes	NNPS
cord-253024-b393ea2u	446	27	of	of	IN
cord-253024-b393ea2u	446	28	Health	Health	NNP
cord-253024-b393ea2u	446	29	(	(	-LRB-
cord-253024-b393ea2u	446	30	A123946	A123946	NNP
cord-253024-b393ea2u	446	31	)	)	-RRB-
cord-253024-b393ea2u	446	32	.	.	.
cord-253024-b393ea2u	447	1	This	this	DT
cord-253024-b393ea2u	447	2	work	work	NN
cord-253024-b393ea2u	447	3	was	be	VBD
cord-253024-b393ea2u	447	4	done	do	VBN
cord-253024-b393ea2u	447	5	during	during	IN
cord-253024-b393ea2u	447	6	the	the	DT
cord-253024-b393ea2u	447	7	tenure	tenure	NN
cord-253024-b393ea2u	447	8	of	of	IN
cord-253024-b393ea2u	447	9	an	an	DT
cord-253024-b393ea2u	447	10	Established	Established	NNP
cord-253024-b393ea2u	447	11	Investigator	Investigator	NNP
cord-253024-b393ea2u	447	12	of	of	IN
cord-253024-b393ea2u	447	13	the	the	DT
cord-253024-b393ea2u	447	14	American	American	NNP
cord-253024-b393ea2u	447	15	Heart	Heart	NNP
cord-253024-b393ea2u	447	16	Association	Association	NNP
cord-253024-b393ea2u	447	17	(	(	-LRB-
cord-253024-b393ea2u	447	18	AHA	AHA	NNP
cord-253024-b393ea2u	447	19	89	89	CD
cord-253024-b393ea2u	447	20	-	-	HYPH
cord-253024-b393ea2u	447	21	0913	0913	CD
cord-253024-b393ea2u	447	22	)	)	-RRB-
cord-253024-b393ea2u	448	1	(	(	-LRB-
cord-253024-b393ea2u	448	2	R.S.B.	R.S.B.	NNP
cord-253024-b393ea2u	448	3	)	)	-RRB-
cord-253024-b393ea2u	448	4	.	.	.